NO301596B1 - Fremgangsmåte for fremstilling av lyofiliserte pattedyrceller - Google Patents
Fremgangsmåte for fremstilling av lyofiliserte pattedyrceller Download PDFInfo
- Publication number
- NO301596B1 NO301596B1 NO911533A NO911533A NO301596B1 NO 301596 B1 NO301596 B1 NO 301596B1 NO 911533 A NO911533 A NO 911533A NO 911533 A NO911533 A NO 911533A NO 301596 B1 NO301596 B1 NO 301596B1
- Authority
- NO
- Norway
- Prior art keywords
- cells
- cell
- lyophilized
- trehalose
- cell pellet
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 58
- 210000004962 mammalian cell Anatomy 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title description 14
- 210000004027 cell Anatomy 0.000 claims abstract description 142
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 28
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 28
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 28
- 239000008188 pellet Substances 0.000 claims abstract description 15
- 239000012530 fluid Substances 0.000 claims abstract description 11
- 238000007710 freezing Methods 0.000 claims abstract description 11
- 230000008014 freezing Effects 0.000 claims abstract description 11
- 238000004108 freeze drying Methods 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 11
- 239000006285 cell suspension Substances 0.000 claims description 10
- 210000000170 cell membrane Anatomy 0.000 claims description 9
- 108010088751 Albumins Proteins 0.000 claims description 8
- 102000009027 Albumins Human genes 0.000 claims description 8
- 239000000644 isotonic solution Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000011065 in-situ storage Methods 0.000 claims description 3
- 230000002829 reductive effect Effects 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 abstract description 9
- 238000003860 storage Methods 0.000 abstract description 6
- 230000002489 hematologic effect Effects 0.000 abstract description 4
- 210000004408 hybridoma Anatomy 0.000 abstract description 3
- 238000003018 immunoassay Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 16
- 239000002502 liposome Substances 0.000 description 16
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 14
- 238000003556 assay Methods 0.000 description 13
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
- 210000003719 b-lymphocyte Anatomy 0.000 description 12
- 239000012528 membrane Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 238000000149 argon plasma sintering Methods 0.000 description 9
- 238000010186 staining Methods 0.000 description 9
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 238000000684 flow cytometry Methods 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 150000002632 lipids Chemical class 0.000 description 6
- 241000283707 Capra Species 0.000 description 5
- 210000000601 blood cell Anatomy 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 230000009257 reactivity Effects 0.000 description 5
- 210000002421 cell wall Anatomy 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- 230000002335 preservative effect Effects 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 108010052285 Membrane Proteins Proteins 0.000 description 3
- 102000018697 Membrane Proteins Human genes 0.000 description 3
- 230000022131 cell cycle Effects 0.000 description 3
- 239000002577 cryoprotective agent Substances 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 239000004005 microsphere Substances 0.000 description 3
- 210000004976 peripheral blood cell Anatomy 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000002458 cell surface marker Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000007822 cytometric assay Methods 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 239000000592 Artificial Cell Substances 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000012911 assay medium Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000006369 cell cycle progression Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000010324 immunological assay Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000007814 microscopic assay Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 229940021222 peritoneal dialysis isotonic solution Drugs 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 108060006184 phycobiliprotein Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- -1 trehalose Chemical class 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Dentistry (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/260,260 US5059518A (en) | 1988-10-20 | 1988-10-20 | Stabilized lyophilized mammalian cells and method of making same |
| PCT/US1989/004522 WO1990004329A1 (en) | 1988-10-20 | 1989-10-11 | Stabilized lyophilized mammalian cells and method of making same |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO911533D0 NO911533D0 (no) | 1991-04-18 |
| NO911533L NO911533L (no) | 1991-05-27 |
| NO301596B1 true NO301596B1 (no) | 1997-11-17 |
Family
ID=22988452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO911533A NO301596B1 (no) | 1988-10-20 | 1991-04-18 | Fremgangsmåte for fremstilling av lyofiliserte pattedyrceller |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US5059518A (es) |
| EP (1) | EP0444159B1 (es) |
| JP (1) | JP2912400B2 (es) |
| CN (1) | CN1038691C (es) |
| AT (1) | ATE199625T1 (es) |
| AU (1) | AU633029B2 (es) |
| CA (1) | CA1335362C (es) |
| DE (1) | DE68929286T2 (es) |
| ES (1) | ES2019727A6 (es) |
| IE (1) | IE62544B1 (es) |
| IL (1) | IL92057A0 (es) |
| MX (1) | MX166481B (es) |
| NO (1) | NO301596B1 (es) |
| WO (1) | WO1990004329A1 (es) |
| ZA (1) | ZA897924B (es) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5759774A (en) * | 1988-05-18 | 1998-06-02 | Cobe Laboratories, Inc. | Method of detecting circulating antibody types using dried or lyophilized cells |
| US5958670A (en) * | 1988-05-18 | 1999-09-28 | Cobe Laboratories, Inc. | Method of freezing cells and cell-like materials |
| IL90188A0 (en) * | 1988-05-18 | 1989-12-15 | Cryopharm Corp | Process and medium for the lyophilization of erythrocytes |
| US5045446A (en) * | 1988-08-26 | 1991-09-03 | Cryopharm Corporation | Lyophilization of cells |
| IE76319B1 (en) * | 1990-07-23 | 1997-10-22 | Becton Dickinson Co | Preservation of cells as controls or standards in cellular analysis |
| EP0522134A1 (en) * | 1991-01-11 | 1993-01-13 | COBE Laboratories, Inc. | Method of detecting circulating antibody types using dried or lyophilized cells or cell-like material |
| US5242792A (en) * | 1991-02-25 | 1993-09-07 | The United States Of America As Represented By The Secretary Of The Navy | Method for the preservation of red blood cells by lyophilization using glycerol or inositol with disaccharides |
| US5459073A (en) * | 1991-05-08 | 1995-10-17 | Streck Laboratories, Inc. | Method and composition for preserving antigens and process for utilizing cytological material produced by same |
| US5849517A (en) * | 1991-05-08 | 1998-12-15 | Streck Laboratories, Inc. | Method and composition for preserving antigens and nucleic acids and process for utilizing cytological material produced by same |
| US5460797A (en) * | 1991-05-08 | 1995-10-24 | Streck Laboratories, Inc. | Method for fixing tissues and cells for analysis using oxazolidine compounds |
| JPH05142122A (ja) * | 1991-11-15 | 1993-06-08 | Olympus Optical Co Ltd | 固相細胞の乾燥固定方法 |
| JPH07507443A (ja) * | 1992-01-21 | 1995-08-24 | コーベ ラボラトリーズ インコーポレイテッド | 細胞および細胞様物質を凍結する方法 |
| EP0642301B1 (en) * | 1992-05-29 | 2003-03-12 | The University Of North Carolina At Chapel Hill | Pharmaceutically acceptable fixed-dried human blood platelets |
| US5342754A (en) * | 1992-09-14 | 1994-08-30 | Coulter Corporation | Preserved, non-infectious control cell for use in the identification of a disease through blood testing |
| US5763204A (en) * | 1992-09-14 | 1998-06-09 | Coulter Corporation | Preparation of preserved, non-infectious control cell for use in the identification of a disease through blood testing |
| BE1006379A3 (fr) * | 1992-11-26 | 1994-08-09 | Raymond Gilles | Procede de conservation de cellules vivantes, d'ensembles de cellules et/ou de derives de ces cellules par lyophilisation. |
| US6005100A (en) * | 1992-12-02 | 1999-12-21 | Kabushiki Kaisha Hayashibara Seitbutsu Kagaku Kenkyujo | Trehalose composition for prolonging product shelf life |
| JP3168550B2 (ja) * | 1992-12-02 | 2001-05-21 | 株式会社林原生物化学研究所 | 脱水剤およびそれを用いる含水物の脱水方法並びにその方法で得られる脱水物品 |
| AU6024294A (en) * | 1993-01-08 | 1994-08-15 | Coulter Corporation | Method of using preserved control cells in the calibration of fluorescent and light scatter measurements |
| US6068874A (en) * | 1993-02-16 | 2000-05-30 | Dehydration Technologies, Inc. | Process of dehydrating biological products |
| US5409826A (en) * | 1993-06-08 | 1995-04-25 | Coulter Corporation | Preserved, non-infectious control cells prepared by the modulation or modification of normal cells |
| WO1995011313A1 (en) * | 1993-10-18 | 1995-04-27 | Amoco Corporation | Control compositions for determination of molecular cytogenetic abnormalities with dna probes |
| JP4142095B2 (ja) | 1994-06-02 | 2008-08-27 | クアドラント・ドラツグ・デリバリー・リミテツド | 各種物質の再水和または融解時の凝集を防止する方法及び該方法により得られる組成物 |
| US5477891A (en) * | 1994-07-08 | 1995-12-26 | Benesi; Steve C. | Woven filter fabric |
| US5759864A (en) * | 1995-06-23 | 1998-06-02 | Cedars Sinai Medical Center | Methods for reducing background binding in antibody preparations |
| US5690963A (en) * | 1995-06-30 | 1997-11-25 | The United States Of America As Represented By The Secretary Of The Navy | Freeze dried red blood cells |
| US5736313A (en) * | 1995-10-20 | 1998-04-07 | The United States Of America As Represented By The Secretary Of The Navy | Method of lyophilizing platelets by incubation with high carbohydrate concentrations and supercooling prior to freezing |
| US6274369B1 (en) * | 1996-02-02 | 2001-08-14 | Invitrogen Corporation | Method capable of increasing competency of bacterial cell transformation |
| AU2590997A (en) | 1996-03-29 | 1997-10-22 | Life Technologies, Inc. | Method for increasing viability and transformation efficiency of bacteria during storage at low temperatures |
| US6268012B1 (en) | 1996-06-07 | 2001-07-31 | Dtl S.A. | Dried product and a drying process |
| US5968831A (en) * | 1996-10-29 | 1999-10-19 | Coulter International Corp. | Cell control used to confirm enzymatic activity |
| US6221575B1 (en) | 1997-02-07 | 2001-04-24 | Quadrant Holdings Cambridge Ltd. | Methods for producing dried storage-stable platelets and compositions obtained thereby |
| DE69829976T2 (de) | 1997-02-12 | 2006-04-27 | Invitrogen Corp., Carlsbad | Verfahren zur herstellung von zellen |
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- 1989-10-11 JP JP1510932A patent/JP2912400B2/ja not_active Expired - Lifetime
- 1989-10-11 WO PCT/US1989/004522 patent/WO1990004329A1/en not_active Ceased
- 1989-10-11 AT AT90906036T patent/ATE199625T1/de not_active IP Right Cessation
- 1989-10-11 EP EP90906036A patent/EP0444159B1/en not_active Expired - Lifetime
- 1989-10-11 AU AU44118/89A patent/AU633029B2/en not_active Ceased
- 1989-10-11 DE DE68929286T patent/DE68929286T2/de not_active Expired - Lifetime
- 1989-10-17 IE IE334189A patent/IE62544B1/en not_active IP Right Cessation
- 1989-10-19 ES ES8903520A patent/ES2019727A6/es not_active Expired - Fee Related
- 1989-10-19 ZA ZA897924A patent/ZA897924B/xx unknown
- 1989-10-20 CN CN89107981A patent/CN1038691C/zh not_active Expired - Fee Related
- 1989-10-20 IL IL92057A patent/IL92057A0/xx not_active IP Right Cessation
- 1989-10-20 MX MX018040A patent/MX166481B/es unknown
-
1991
- 1991-04-18 NO NO911533A patent/NO301596B1/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO911533D0 (no) | 1991-04-18 |
| CN1041974A (zh) | 1990-05-09 |
| WO1990004329A1 (en) | 1990-05-03 |
| ATE199625T1 (de) | 2001-03-15 |
| ES2019727A6 (es) | 1991-07-01 |
| JP2912400B2 (ja) | 1999-06-28 |
| DE68929286T2 (de) | 2001-10-31 |
| AU633029B2 (en) | 1993-01-21 |
| ZA897924B (en) | 1991-06-26 |
| AU4411889A (en) | 1990-05-14 |
| EP0444159A1 (en) | 1991-09-04 |
| CN1038691C (zh) | 1998-06-10 |
| CA1335362C (en) | 1995-04-25 |
| JPH04501112A (ja) | 1992-02-27 |
| DE68929286D1 (de) | 2001-04-19 |
| EP0444159A4 (en) | 1992-05-06 |
| IL92057A0 (en) | 1990-07-12 |
| MX166481B (es) | 1993-01-12 |
| IE62544B1 (en) | 1995-02-08 |
| US5059518A (en) | 1991-10-22 |
| NO911533L (no) | 1991-05-27 |
| EP0444159B1 (en) | 2001-03-14 |
| IE893341L (en) | 1990-04-20 |
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