ES2019727A6 - Un procedimiento para la preparacion de celulas de mamifero liofilizadas. - Google Patents

Un procedimiento para la preparacion de celulas de mamifero liofilizadas.

Info

Publication number
ES2019727A6
ES2019727A6 ES8903520A ES8903520A ES2019727A6 ES 2019727 A6 ES2019727 A6 ES 2019727A6 ES 8903520 A ES8903520 A ES 8903520A ES 8903520 A ES8903520 A ES 8903520A ES 2019727 A6 ES2019727 A6 ES 2019727A6
Authority
ES
Spain
Prior art keywords
cells
mammalian cells
lyophilizing
making same
retain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8903520A
Other languages
English (en)
Inventor
Robert H Raynor
Jr Steven Healy
Kenneth Kortright
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Corp
Original Assignee
Coulter Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Corp filed Critical Coulter Corp
Publication of ES2019727A6 publication Critical patent/ES2019727A6/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04Preserving or maintaining viable microorganisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666Particle count or volume standard or control [e.g., platelet count standards, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

UN METODO DE LIOFILIZACION DE CELULAS DE MAMIFERO PARA PRODUCIR CELULAS HUMANAS, LINEAS DE CELULAS DE HIBRIDOMA, CELULAS DE TEJIDOS Y CELULAS DE CONTROL, PRESERVADAS, PARA INMUNOENSAYOS Y OTRAS MEDIDAS HEMATOLOGICAS. ANTES DE CONGELAR Y LIOFILIZAR, EL SEDIMENTO DE CELULAS DE MAMIFERO PREPARADO SE SUSPENDE EN UNA SOLUCION DE TREHALOSA EN UN FLUIDO ISOTONICO PREPARADO A UNA CONCENTRACION OPTIMA ESPECIFICA Y SE INCUBA A LA TEMPERATURA AMBIENTE DURANTE EL PERIODO DE TIEMPO INDICADO.
ES8903520A 1988-10-20 1989-10-19 Un procedimiento para la preparacion de celulas de mamifero liofilizadas. Expired - Fee Related ES2019727A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/260,260 US5059518A (en) 1988-10-20 1988-10-20 Stabilized lyophilized mammalian cells and method of making same

Publications (1)

Publication Number Publication Date
ES2019727A6 true ES2019727A6 (es) 1991-07-01

Family

ID=22988452

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8903520A Expired - Fee Related ES2019727A6 (es) 1988-10-20 1989-10-19 Un procedimiento para la preparacion de celulas de mamifero liofilizadas.

Country Status (15)

Country Link
US (1) US5059518A (es)
EP (1) EP0444159B1 (es)
JP (1) JP2912400B2 (es)
CN (1) CN1038691C (es)
AT (1) ATE199625T1 (es)
AU (1) AU633029B2 (es)
CA (1) CA1335362C (es)
DE (1) DE68929286T2 (es)
ES (1) ES2019727A6 (es)
IE (1) IE62544B1 (es)
IL (1) IL92057A0 (es)
MX (1) MX166481B (es)
NO (1) NO301596B1 (es)
WO (1) WO1990004329A1 (es)
ZA (1) ZA897924B (es)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL90188A0 (en) * 1988-05-18 1989-12-15 Cryopharm Corp Process and medium for the lyophilization of erythrocytes
US5759774A (en) * 1988-05-18 1998-06-02 Cobe Laboratories, Inc. Method of detecting circulating antibody types using dried or lyophilized cells
US5958670A (en) * 1988-05-18 1999-09-28 Cobe Laboratories, Inc. Method of freezing cells and cell-like materials
US5045446A (en) * 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
IE76319B1 (en) * 1990-07-23 1997-10-22 Becton Dickinson Co Preservation of cells as controls or standards in cellular analysis
CA2075928A1 (en) * 1991-01-11 1992-07-12 Roger W. Hackett Method of detecting circulating antibody types using dried or lyophilized cells or cell-like material
US5242792A (en) * 1991-02-25 1993-09-07 The United States Of America As Represented By The Secretary Of The Navy Method for the preservation of red blood cells by lyophilization using glycerol or inositol with disaccharides
US5460797A (en) * 1991-05-08 1995-10-24 Streck Laboratories, Inc. Method for fixing tissues and cells for analysis using oxazolidine compounds
US5459073A (en) * 1991-05-08 1995-10-17 Streck Laboratories, Inc. Method and composition for preserving antigens and process for utilizing cytological material produced by same
US5849517A (en) * 1991-05-08 1998-12-15 Streck Laboratories, Inc. Method and composition for preserving antigens and nucleic acids and process for utilizing cytological material produced by same
JPH05142122A (ja) * 1991-11-15 1993-06-08 Olympus Optical Co Ltd 固相細胞の乾燥固定方法
CA2128527A1 (en) * 1992-01-21 1993-07-22 Raymond P. Goodrich, Jr. Method of freezing cells and cell-like materials
PT642301E (pt) * 1992-05-29 2003-06-30 Univ East Carolina Plaquetas de sangue humano fixas secas farmaceuticamente aceitaveis
US5763204A (en) * 1992-09-14 1998-06-09 Coulter Corporation Preparation of preserved, non-infectious control cell for use in the identification of a disease through blood testing
US5342754A (en) * 1992-09-14 1994-08-30 Coulter Corporation Preserved, non-infectious control cell for use in the identification of a disease through blood testing
BE1006379A3 (fr) * 1992-11-26 1994-08-09 Raymond Gilles Procede de conservation de cellules vivantes, d'ensembles de cellules et/ou de derives de ces cellules par lyophilisation.
US6005100A (en) * 1992-12-02 1999-12-21 Kabushiki Kaisha Hayashibara Seitbutsu Kagaku Kenkyujo Trehalose composition for prolonging product shelf life
JP3168550B2 (ja) * 1992-12-02 2001-05-21 株式会社林原生物化学研究所 脱水剤およびそれを用いる含水物の脱水方法並びにその方法で得られる脱水物品
WO1994016314A1 (en) * 1993-01-08 1994-07-21 Coulter Corporation Method of using preserved control cells in the calibration of fluorescent and light scatter measurements
US6068874A (en) * 1993-02-16 2000-05-30 Dehydration Technologies, Inc. Process of dehydrating biological products
US5409826A (en) * 1993-06-08 1995-04-25 Coulter Corporation Preserved, non-infectious control cells prepared by the modulation or modification of normal cells
WO1995011313A1 (en) * 1993-10-18 1995-04-27 Amoco Corporation Control compositions for determination of molecular cytogenetic abnormalities with dna probes
CN1188171C (zh) * 1994-06-02 2005-02-09 廓德伦特控股剑桥有限公司 防止各种特质在再水化或熔化时聚集的方法以及由此获得的组合物
US5477891A (en) * 1994-07-08 1995-12-26 Benesi; Steve C. Woven filter fabric
US5759864A (en) * 1995-06-23 1998-06-02 Cedars Sinai Medical Center Methods for reducing background binding in antibody preparations
US5690963A (en) * 1995-06-30 1997-11-25 The United States Of America As Represented By The Secretary Of The Navy Freeze dried red blood cells
US5736313A (en) * 1995-10-20 1998-04-07 The United States Of America As Represented By The Secretary Of The Navy Method of lyophilizing platelets by incubation with high carbohydrate concentrations and supercooling prior to freezing
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
EP0921814A4 (en) 1996-03-29 2001-10-04 Life Technologies Inc Process for increasing the viability and transformation efficiency of bacteria during storage at low temperatures
US6268012B1 (en) 1996-06-07 2001-07-31 Dtl S.A. Dried product and a drying process
US5968831A (en) * 1996-10-29 1999-10-19 Coulter International Corp. Cell control used to confirm enzymatic activity
DK0967862T3 (da) 1997-02-07 2003-05-12 Elan Drug Delivery Ltd Fremgangsmåde og præparater til fremstilling af tørrede, lagringsstabile blodplader
ES2241120T3 (es) 1997-02-12 2005-10-16 Invitrogen Corporation Metodos para liofilizar celulas competentes.
EP0868916A3 (en) * 1997-03-04 2004-09-15 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Reduction inhibitory agent for active-oxygen eliminating activity
US6071745A (en) * 1997-06-27 2000-06-06 Bio-Rad Laboratories Method and formulation for lyophilizing cultured human cells to preserve RNA and DNA contained in cells for use in molecular biology experiments
US7282350B2 (en) 1998-02-12 2007-10-16 Immunivest Corporation Labeled cell sets for use as functional controls in rare cell detection assays
US20010018192A1 (en) 1998-02-12 2001-08-30 Terstappen Leon W.M.M. Labeled cells for use as an internal functional control in rare cell detection assays
US20020102208A1 (en) * 1999-03-01 2002-08-01 Paul Chinn Radiolabeling kit and binding assay
MY133346A (en) * 1999-03-01 2007-11-30 Biogen Inc Kit for radiolabeling ligands with yttrium-90
CA2397362A1 (en) * 2000-02-10 2001-08-16 The Regents Of The University Of California Therapeutic platelets and methods
US6770478B2 (en) 2000-02-10 2004-08-03 The Regents Of The University Of California Erythrocytic cells and method for preserving cells
US20020076445A1 (en) * 2000-02-10 2002-06-20 Crowe John H. Eukaryotic cells and method for preserving cells
EP1428008B1 (en) * 2001-09-06 2012-01-18 Core Dynamics Limited Improved method for freezing viable cells
AU2002331847A1 (en) * 2001-09-14 2003-04-01 Invitrogen Corporation Composition for stabilizing biological materials
US7659111B2 (en) * 2002-06-27 2010-02-09 Core Dynamics Limited Method for freezing viable cells
KR100476790B1 (ko) * 2002-09-13 2005-03-16 주식회사 제넨메드 세포 저장 용액과 이를 이용한 동물 세포의 동결 및 저장방법
FR2852329A1 (fr) * 2003-03-14 2004-09-17 Univ Aix Marseille Ii Cellules lyophilisees et procede de lyophilisation permettant l'obtention desdites cellules
ATE494777T1 (de) * 2003-10-09 2011-01-15 Core Dynamics Ltd Verfahren zum tiefgefrieren, auftauen und transplantieren von lebensfähigem knorpel
US20050287513A1 (en) * 2003-10-30 2005-12-29 Davis Ashley S Method for preserving intracellular molecular detail
EP1711053A2 (en) * 2004-02-02 2006-10-18 I.M.T. Interface Multigrad Technology Ltd. Biological material and methods and solutions for preservation thereof
WO2005072790A1 (en) * 2004-02-02 2005-08-11 I.M.T. Interface Multigrad Technology Ltd. Device for directional cooling of biological matter
JP5096148B2 (ja) * 2004-06-07 2012-12-12 コア・ダイナミクス・リミテッド 生物学的試料の殺菌方法
US8037696B2 (en) * 2004-08-12 2011-10-18 Core Dynamics Limited Method and apparatus for freezing or thawing of a biological material
EP1850661A2 (en) * 2005-02-22 2007-11-07 Interface Multigrad Technology (IMT) Ltd. Preserved viable cartilage, method for its preservation, and system and devices used therefor
US20070015134A1 (en) * 2005-07-15 2007-01-18 Beckman Coulter, Inc. Quantitative stabilized cell reference control products and methods
EP1909565A2 (en) * 2005-08-03 2008-04-16 Interface Multigrad Technology (IMT) Ltd. Somatic cells for use in cell therapy
US20090202978A1 (en) * 2008-02-13 2009-08-13 Ginadi Shaham Method and apparatus for freezing of a biological material
JP6043934B2 (ja) 2008-10-31 2016-12-14 国立大学法人名古屋大学 抗原特異的細胞傷害性t細胞の調製キット
ES2648139T3 (es) 2010-02-19 2017-12-28 Cadila Pharmaceuticals Ltd. Una composición farmacéutica de células muertas con inmunogenicidad sustancialmente retenida
CN104082277B (zh) * 2014-07-25 2016-04-13 成都清科生物科技有限公司 一种人外周血单个核细胞的冻存保护剂及保存方法
US20170332623A1 (en) * 2016-03-28 2017-11-23 Cook General Biotechnology Llc Viable cell compositions, and methods related to same
CN106821938B (zh) * 2017-03-21 2020-03-24 北京海康殷氏生物科技有限责任公司 一种人间充质干细胞冻干粉的制备方法
JP7110360B2 (ja) 2017-10-09 2022-08-01 テルモ ビーシーティー バイオテクノロジーズ,エルエルシー 凍結乾燥方法
CA3121200A1 (en) 2018-11-30 2020-06-04 Cellphire, Inc. Platelets as delivery agents
US11767511B2 (en) 2018-11-30 2023-09-26 Cellphire, Inc. Platelets as delivery agents
JP7495426B2 (ja) 2019-03-14 2024-06-04 テルモ ビーシーティー バイオテクノロジーズ,エルエルシー 凍結乾燥容器用充填治具、システム及び使用方法
SG11202112054WA (en) 2019-05-03 2021-11-29 Cellphire Inc Materials and methods for producing blood products
CA3150936A1 (en) 2019-08-16 2021-02-25 Cellphire, Inc. Thrombosomes as an antiplatelet agent reversal agent
CN112970739A (zh) * 2019-12-18 2021-06-18 陕西光子动力航天科技有限公司 一种细胞或干细胞冻存制备方法
CA3170198A1 (en) 2020-02-04 2021-08-12 Cellphire, Inc Methods of treating acquired hemophilia with anti-fibrinolytic loaded platelets
CN111351930A (zh) * 2020-02-20 2020-06-30 泛肽生物科技(浙江)有限公司 一种活性人淋巴细胞表面抗原质控品及其制备方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2025718C3 (de) * 1967-11-13 1979-06-13 Abbott Laboratories, Chicago, Ill. (V.St.A.) Verfahren zum Stabilisieren von lyophillsierten Erythrozyten
DE2551208C3 (de) * 1975-11-14 1981-05-27 Behringwerke Ag, 3550 Marburg Verfahren zur Herstellung einer stabilen Erythrozyten-Präparation
JPS5612317A (en) * 1979-07-10 1981-02-06 Kenji Sato Retention of fresh tissue transplantable by injection
JPS577419A (en) * 1980-06-17 1982-01-14 Toshiba Kagaku Kogyo Kk Erythrocyte storing solution
JPS58131913A (ja) * 1982-01-29 1983-08-06 Green Cross Corp:The 赤血球凍結乾燥物およびその製造方法
CA1202903A (en) * 1982-04-29 1986-04-08 Edgar E. Ribi Stable composition and preparation thereof
JPS59109862A (ja) * 1982-12-15 1984-06-25 Takeda Chem Ind Ltd 凍結乾燥感作血球の製造法
GB2187191B (en) * 1984-01-30 1989-11-01 Quadrant Bioresources Ltd Protection of proteins and the like
US4649106A (en) * 1984-06-01 1987-03-10 Dana-Farber Cancer Institute, Inc. Distinguishing subsets of human cells
WO1986003938A1 (en) * 1985-01-11 1986-07-17 The Regents Of The University Of California Method for preserving liposomes
US4891319A (en) * 1985-07-09 1990-01-02 Quadrant Bioresources Limited Protection of proteins and the like
GB8604983D0 (en) * 1986-02-28 1986-04-09 Biocompatibles Ltd Protein preservation
FR2625221B1 (fr) * 1987-12-24 1990-06-15 Sanofi Sa Protoplastes stabilises et procede pour leur obtention
US5045446A (en) * 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
US4874690A (en) * 1988-08-26 1989-10-17 Cryopharm Corporation Lyophilization of red blood cells

Also Published As

Publication number Publication date
JP2912400B2 (ja) 1999-06-28
CA1335362C (en) 1995-04-25
NO301596B1 (no) 1997-11-17
CN1041974A (zh) 1990-05-09
NO911533L (no) 1991-05-27
EP0444159B1 (en) 2001-03-14
ATE199625T1 (de) 2001-03-15
JPH04501112A (ja) 1992-02-27
IE62544B1 (en) 1995-02-08
DE68929286T2 (de) 2001-10-31
AU633029B2 (en) 1993-01-21
WO1990004329A1 (en) 1990-05-03
EP0444159A1 (en) 1991-09-04
AU4411889A (en) 1990-05-14
MX166481B (es) 1993-01-12
NO911533D0 (no) 1991-04-18
CN1038691C (zh) 1998-06-10
US5059518A (en) 1991-10-22
IL92057A0 (en) 1990-07-12
DE68929286D1 (de) 2001-04-19
EP0444159A4 (en) 1992-05-06
IE893341L (en) 1990-04-20
ZA897924B (en) 1991-06-26

Similar Documents

Publication Publication Date Title
ES2019727A6 (es) Un procedimiento para la preparacion de celulas de mamifero liofilizadas.
Somero et al. Biochemical adaptation to the environment
FR2559780B1 (fr) Systemes biocompatibles implantables a base de collagene permettant la conservation et/ou la culture cellulaires et/ou la liberation controlee de principes actifs
Lin et al. Na+, K+-ATPase enzyme units in skeletal muscle from lean and obese mice
NOVALES et al. Melanin-Dispersing Effect of Adenosine 3 ″, 5 ″-Monophosphate on Amphibian Melanophores
Dakshinamurti et al. Requirement for biotin and the function of biotin in cells in culture
EP0284409A3 (en) Active specific immune suppression
ES2008404A6 (es) Procedimiento para congelar, almacenar y descongelar tejido rico en colageno tal como valvulas de corazon.
WO2000006704A3 (en) Hematopoietic cell composition for use in transplantation
IL92703A0 (en) Cell culture medium and its use in culturing mammalian cells
SE8500621D0 (sv) Forbettrad proteinframstellning med anvendning av hypertoniska medier
Waldron et al. Evidence for a high proportion of inactive ribosomes in slow-growing yeast cells
DK363088A (da) Dobbeltstrenget rna-korrektion af afvigende metaboliske baner, der staar i forbindelse med ukontrollerede tumorcelle- og virusvaekstcykler
JPS5739797A (en) Preparation of human chorionic gonadotropin
ES8800757A1 (es) Procedimiento para preparar un reactivo para valorar por hemaglutinacion anticuerpos dirigidos contra toxinas bacterianas
DE3272969D1 (en) Anaerobic method for preserving whole blood, tissue and components containing living mammalian cells
Avissar Induction of nitrate assimilation in the cyanobacterium Anabaena variabilis
VOTTA et al. The mechanism of onset of the stationary phase in Euglena gracilis grown with 10 mm succinate: Intracellular pH values
EP0249772A3 (en) Method and composition for plant tissue and cell culture
Sugarman et al. [14C]-Pantothenate Accumulation by Isolated Adipocytes from Adult Rats of Different Ages
Lane Use of Carbon-14-labelled Arginine to measure Catabolic Rates of Proteins and the Recycling of Amino-acids
SU1193168A1 (ru) Способ получени культуры клеток эпидермиса человека
Saxén In vitro model-systems for chemical teratogenesis.
Weiner Biotin uptake, utilization and efflux in normal and biotin deficiency states.
Wilkinson Jr et al. Effect of feeding dried egg plant (Solanum melonga L.) on plasma cholesterol.

Legal Events

Date Code Title Description
SA6 Expiration date (snapshot 920101)

Free format text: 2009-10-19

FD1A Patent lapsed

Effective date: 20061020