MXPA02008485A - Procedimiento para producir peptidos usando un sistema de transcripcion/traduccion in vitro. - Google Patents

Procedimiento para producir peptidos usando un sistema de transcripcion/traduccion in vitro.

Info

Publication number
MXPA02008485A
MXPA02008485A MXPA02008485A MXPA02008485A MXPA02008485A MX PA02008485 A MXPA02008485 A MX PA02008485A MX PA02008485 A MXPA02008485 A MX PA02008485A MX PA02008485 A MXPA02008485 A MX PA02008485A MX PA02008485 A MXPA02008485 A MX PA02008485A
Authority
MX
Mexico
Prior art keywords
reaction system
vitro
transcription
peptide
translating
Prior art date
Application number
MXPA02008485A
Other languages
English (en)
Inventor
Akio Inoue
Original Assignee
Post Genome Inst Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Post Genome Inst Co Ltd filed Critical Post Genome Inst Co Ltd
Publication of MXPA02008485A publication Critical patent/MXPA02008485A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se pretende construir un sistema de reaccion por medio del cual un peptido producido en un sistema de sintesis de peptido in vitro pueda ser aislado eficientemente en una alta pureza del sistema de reaccion y, al mismo tiempo, resolver el problema de consumo de energia en el sistema de reaccion; un procedimiento para producir un peptido o un derivado de peptido usando un sistema de reaccion para transcribir un ADN en un ARN y despues traducir el ARN producido, o un sistema de reaccion para traducir un ARN in vitro, caracterizado porque algunos o todos los componentes de proteina que constituyen el sistema de reaccion de transcripcion/traduccion estan marcados con una sustancia de un par de sustancias que se adhieren entre si, y la otra sustancia se usa como un adsorbente para capturar despues de la traduccion dichos componentes de proteina marcados.
MXPA02008485A 2000-12-28 2001-12-06 Procedimiento para producir peptidos usando un sistema de transcripcion/traduccion in vitro. MXPA02008485A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2000401417 2000-12-28
JP2001006910 2001-01-15
JP2001227094 2001-07-27
JP2001294795A JP4061043B2 (ja) 2000-12-28 2001-09-26 ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法
PCT/JP2001/010682 WO2002053582A2 (en) 2000-12-28 2001-12-06 Process for producing peptides by using in vitrotranscription/translation system

Publications (1)

Publication Number Publication Date
MXPA02008485A true MXPA02008485A (es) 2004-05-17

Family

ID=27481947

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02008485A MXPA02008485A (es) 2000-12-28 2001-12-06 Procedimiento para producir peptidos usando un sistema de transcripcion/traduccion in vitro.

Country Status (17)

Country Link
US (1) US7118883B2 (es)
EP (1) EP1319074B1 (es)
JP (1) JP4061043B2 (es)
KR (1) KR20030001371A (es)
AT (1) ATE296885T1 (es)
AU (1) AU2002221071A1 (es)
CA (1) CA2400735C (es)
DE (2) DE1319074T1 (es)
ES (1) ES2193896T3 (es)
HU (1) HUP0301723A2 (es)
IL (1) IL150892A0 (es)
IS (1) IS6607A (es)
MX (1) MXPA02008485A (es)
NO (1) NO20024082L (es)
PL (1) PL366364A1 (es)
TR (1) TR200301277T3 (es)
WO (1) WO2002053582A2 (es)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977150B2 (en) * 2001-01-25 2005-12-20 Anthony C. Forster Process and compositions for peptide, protein and peptidomimetic synthesis
DE10137792A1 (de) * 2001-08-06 2003-02-27 Erdmann Volker Verfahren zur Genexpression
WO2004111203A1 (ja) * 2003-06-10 2004-12-23 Shimadzu Corporation 哺乳動物培養細胞抽出液およびその調製方法、ならびに該抽出液を用いた無細胞系タンパク質合成方法
US20040265921A1 (en) * 2003-06-30 2004-12-30 National University Of Singapore Intein-mediated attachment of ligands to proteins for immobilization onto a support
DE10336705A1 (de) * 2003-08-06 2005-03-10 Rina Netzwerk Rna Technologien Verfahren zur Herstellung eines Lysats zur zellfreien Proteinbiosynthese
US7459290B1 (en) 2004-03-30 2008-12-02 Iowa State University Research Foundation, Inc. Methods of using functional 30S subunits
KR20070015216A (ko) * 2004-04-30 2007-02-01 가부시키가이샤 포스트 게놈 겐큐쇼 디술피드 가교 형성형 시험관내 단백질 합성 방법
WO2008005529A2 (en) 2006-07-07 2008-01-10 The Trustees Columbia University In The City Of New York Cell-mediated directed evolution
JP4931135B2 (ja) * 2007-05-02 2012-05-16 国立大学法人 東京大学 リボソームディスプレイ
JP2011515066A (ja) * 2007-10-30 2011-05-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高濃度の糖類および酢酸塩を含む培地において改善されたエタノール生産能を有するザイモモナス（ｚｙｍｏｍｏｎａｓ）
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
CA2794522C (en) 2010-04-05 2019-11-26 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2012074029A1 (ja) 2010-12-01 2012-06-07 田辺三菱製薬株式会社 無細胞翻訳系でFabを提示しうるポリヌクレオチド構築物ならびにそれを用いたFabの製造方法およびスクリーニング方法
JP5948673B2 (ja) 2011-03-10 2016-07-06 ジーンフロンティア株式会社 リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法
EP2694709B1 (en) 2011-04-08 2016-09-14 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US11193154B2 (en) 2011-11-04 2021-12-07 Cornell University Prokaryote-based cell-free system for the synthesis of glycoproteins
TWI640541B (zh) 2011-12-28 2018-11-11 中外製藥股份有限公司 Peptide compound having an annular portion and pharmaceutical composition thereof
SG11201500724QA (en) * 2012-08-16 2015-02-27 Synthetic Genomics Inc Digital to biological converter
EP2927312B1 (en) * 2012-11-30 2022-10-19 Bioneer Corporation Apparatus for automatically preparing cell-free proteins and method for preparing proteins using the same
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
EP2972366B1 (en) 2013-03-15 2020-06-17 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
WO2015070037A2 (en) 2013-11-08 2015-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
AU2020328511A1 (en) 2019-08-09 2022-03-03 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
CN116249785A (zh) 2020-06-02 2023-06-09 10X基因组学有限公司 用于抗原-受体的空间转录组学
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US264147A (en) * 1882-09-12 Attachment fob sewing machines
DE152483T1 (de) 1983-08-22 1986-02-27 Kyowa Hakko Kogyo Co., Ltd., Tokio/Tokyo Verfahren zur herstellung von peptiden.
US4710464A (en) 1984-09-27 1987-12-01 Eli Lilly And Company Transcription terminators
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JP2584613B2 (ja) 1985-03-30 1997-02-26 バリベ、マール 組換えｄｎａ技術によってｄｎａ、ｒｎａ、ペプタイド、ポリペプタイド、または蛋白質を取得するための方法
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
SU1441787A1 (ru) 1987-04-29 1990-09-23 Институт Белка Ан Ссср Способ получени пептидов и белков в бесклеточной системе трансл ции
EP0446299A4 (en) 1988-11-18 1992-05-13 The Regents Of The University Of California Method for site-specifically incorporating unnatural amino acids into proteins
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
EP0494955B1 (en) 1989-10-05 1998-07-15 Optein, Inc. Cell-free synthesis and isolation of novel genes and polypeptides
JPH04200390A (ja) 1990-11-30 1992-07-21 Shigeyuki Yokoyama 無細胞ポリペプチド合成系によるポリペプチドの製造方法
US5492817A (en) * 1993-11-09 1996-02-20 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
SE9604108D0 (sv) 1996-11-08 1996-11-08 Maans Ehrenberg A method for the labelling of polypeptides and novel labelled polypeptides
US6306628B1 (en) * 1999-08-25 2001-10-23 Ambergen, Incorporated Methods for the detection, analysis and isolation of Nascent proteins
US6977150B2 (en) 2001-01-25 2005-12-20 Anthony C. Forster Process and compositions for peptide, protein and peptidomimetic synthesis

Also Published As

Publication number Publication date
WO2002053582A2 (en) 2002-07-11
ES2193896T1 (es) 2003-11-16
KR20030001371A (ko) 2003-01-06
JP4061043B2 (ja) 2008-03-12
AU2002221071A1 (en) 2002-07-16
NO20024082D0 (no) 2002-08-27
EP1319074A2 (en) 2003-06-18
EP1319074B1 (en) 2005-06-01
IS6607A (is) 2002-10-31
CA2400735C (en) 2008-03-18
US7118883B2 (en) 2006-10-10
HUP0301723A2 (hu) 2003-08-28
ATE296885T1 (de) 2005-06-15
PL366364A1 (en) 2005-01-24
US20020123101A1 (en) 2002-09-05
TR200301277T3 (tr) 2003-11-21
WO2002053582A3 (en) 2003-03-20
ES2193896T3 (es) 2005-10-16
NO20024082L (no) 2002-08-27
DE60111247T2 (de) 2006-05-04
CA2400735A1 (en) 2002-07-11
DE60111247D1 (de) 2005-07-07
JP2003102495A (ja) 2003-04-08
IL150892A0 (en) 2003-02-12
DE1319074T1 (de) 2003-11-27

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