MX9605357A - Variantes de subtilisina bpn' que tiene absorcion reducida e hidrolisis incrementada. - Google Patents

Variantes de subtilisina bpn' que tiene absorcion reducida e hidrolisis incrementada.

Info

Publication number
MX9605357A
MX9605357A MX9605357A MX9605357A MX9605357A MX 9605357 A MX9605357 A MX 9605357A MX 9605357 A MX9605357 A MX 9605357A MX 9605357 A MX9605357 A MX 9605357A MX 9605357 A MX9605357 A MX 9605357A
Authority
MX
Mexico
Prior art keywords
subtilisin bpn
variants
bpn
loop region
wild
Prior art date
Application number
MX9605357A
Other languages
English (en)
Inventor
Philip Frederick Brode Iii
Bobby Lee Barnett
Donn Nelton Rubingh
Chanchal Kumar Ghosh
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Publication of MX9605357A publication Critical patent/MX9605357A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invencion se refiere a variantes de subtilisina BPN' que tiene una secuencia de aminoácidos modificada de secuencia de aminoácidos tipo silvestre, la secuencia de aminoácidos tipo silvestre comprendiendo una primera region de lazo, una segunda region de lazo, una segunda region de lazo, una tercera region de lazo, una cuarta region de lazo y una quinta region de lazo; en donde la secuencia de aminoácidos modificada comprende diferentes aminoácidos de los que ocurren en la subtilisina BPN' de tipo silvestre (es decir, substitucion) en posiciones específicamente identificadas en una o más de las regiones de lazo por lo que la variante de BPN' tiene adsorcion reducida a, e hidrolisis incrementada de, un substrato insoluble en comparacion con la subtilisina BPN' de tipo silvestre; la presente invencion también se refiere a los genes que codifican dichas variantes de subtilisina BPN'; la presente invencion también se refiere a composiciones que comprenden dichas variantes de subtilisina BPN' para limpiar una variedad de superficies.
MX9605357A 1994-05-02 1995-03-16 Variantes de subtilisina bpn' que tiene absorcion reducida e hidrolisis incrementada. MX9605357A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US23793994A 1994-05-02 1994-05-02
US28746194A 1994-08-11 1994-08-11
US08/394,011 US6436690B1 (en) 1993-09-15 1995-03-03 BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
PCT/US1995/003176 WO1995030010A1 (en) 1994-05-02 1995-03-16 Subtilisin bpn' variants having decreased adsorption and increased hydrolysis

Publications (1)

Publication Number Publication Date
MX9605357A true MX9605357A (es) 1997-12-31

Family

ID=27399035

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9605357A MX9605357A (es) 1994-05-02 1995-03-16 Variantes de subtilisina bpn' que tiene absorcion reducida e hidrolisis incrementada.

Country Status (13)

Country Link
US (1) US6436690B1 (es)
EP (1) EP0758388A1 (es)
JP (1) JPH09511652A (es)
CN (1) CN1151761A (es)
AU (1) AU2159195A (es)
BR (1) BR9507597A (es)
CA (1) CA2189426C (es)
CZ (1) CZ321696A3 (es)
HU (1) HUT75559A (es)
IL (1) IL113048A0 (es)
MX (1) MX9605357A (es)
PE (1) PE27897A1 (es)
WO (1) WO1995030010A1 (es)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE19535082A1 (de) 1995-09-21 1997-03-27 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
DE19615776A1 (de) 1996-04-20 1997-10-23 Henkel Kgaa Löslichkeitsverbessertes Enzymgranulat
DE19636035A1 (de) 1996-09-05 1998-03-12 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
BR9712473B1 (pt) 1996-11-04 2009-08-11 variantes de subtilase e composições.
WO1998020116A1 (en) * 1996-11-04 1998-05-14 Novo Nordisk A/S Subtilase variants and compositions
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US6284246B1 (en) 1997-07-30 2001-09-04 The Procter & Gamble Co. Modified polypeptides with high activity and reduced allergenicity
AU9061798A (en) 1997-08-29 1999-03-22 Novo Nordisk A/S Protease variants and compositions
WO1999011768A1 (en) 1997-08-29 1999-03-11 Novo Nordisk A/S Protease variants and compositions
EP0913458B1 (en) * 1997-10-22 2004-06-16 The Procter & Gamble Company Liquid hard-surface cleaning compositions
ES2368718T3 (es) 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
ATE298791T1 (de) 1997-11-21 2005-07-15 Novozymes As Protease-varianten und zusammensetzungen
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
CA2325568A1 (en) 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
JP2002526434A (ja) * 1998-09-22 2002-08-20 ザ、プロクター、エンド、ギャンブル、カンパニー 水不溶性基質と結合したサブチリシン酵素を含有するパーソナルケア組成物
US6831053B1 (en) 1998-10-23 2004-12-14 The Procter & Gamble Company Bleaching compositions comprising multiply-substituted protease variants
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
DE19857687A1 (de) 1998-12-15 2000-06-21 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Waschmittel
DE69943346D1 (de) 1998-12-18 2011-05-19 Novozymes As Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion
AU772993B2 (en) * 1998-12-18 2004-05-13 Novozymes A/S Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region
EP1183343B2 (en) 1999-05-20 2013-11-27 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
EP1183338B2 (en) † 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 97 and 98
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
EP1183342B2 (en) † 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
ATE407201T1 (de) 1999-05-20 2008-09-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
EP1183339B2 (en) 1999-05-20 2013-03-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
ATE408679T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CA2379729A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Protease conjugates having sterically protected clip sites
AU6085800A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid substitutions in defined epitope regions
BR0012693A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante, de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
EP1212397A1 (en) * 1999-09-09 2002-06-12 The Procter & Gamble Company A detergent composition containing a protease
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
WO2001083761A1 (en) * 2000-04-28 2001-11-08 Novozymes A/S Laccase mutants
US6803222B2 (en) * 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
US7153820B2 (en) 2001-08-13 2006-12-26 Ecolab Inc. Solid detergent composition and method for solidifying a detergent composition
DE10153792A1 (de) * 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
US20030157645A1 (en) * 2001-12-21 2003-08-21 Direvo Bio Tech Ag. Subtilisin variants with improved characteristics
WO2003062381A2 (en) * 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
EP2287320B1 (en) * 2002-01-16 2014-10-01 Danisco US Inc. Multiply-substituted protease variants
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
TWI319007B (en) * 2002-11-06 2010-01-01 Novozymes As Subtilase variants
DE10260903A1 (de) * 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
CA2526341C (en) 2003-05-07 2013-02-19 Novozymes A/S Variant subtilisin enzymes (subtilases)
JP4880469B2 (ja) 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ 洗剤中で改良された安定性を有するプロテアーゼ
EP1700904A1 (en) 2005-03-11 2006-09-13 Unilever N.V. Liquid detergent composition
EP1700907A1 (en) 2005-03-11 2006-09-13 Unilever N.V. Liquid bleaching composition
US20070161531A1 (en) * 2005-07-08 2007-07-12 Novozymes A/S Subtilase variants
WO2007122175A1 (en) 2006-04-20 2007-11-01 Novozymes A/S Savinase variants having an improved wash performance on egg stains
US8093200B2 (en) 2007-02-15 2012-01-10 Ecolab Usa Inc. Fast dissolving solid detergent
BRPI0810863A2 (pt) 2007-04-30 2014-10-07 Danisco Us Inc Genencor Div Uso de hidrolisados de proteína para estabilizar formulações de detergente de metaloprotease.
EP2677023B1 (en) 2007-10-18 2018-09-05 Ecolab Inc. Pressed, waxy, solid cleaning compositions and methods of making them
CN104130993B (zh) * 2008-06-06 2018-10-23 丹尼斯科美国公司 包括微生物蛋白酶变体的组合物和方法
CA2743123A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
MX338884B (es) * 2009-12-09 2016-05-04 Danisco Us Inc Composiciones y metodos que comprenden variantes de proteasa.
CN101824467B (zh) * 2009-12-29 2012-07-18 广州益善生物技术有限公司 Cyp2d6基因突变检测液相芯片及检测方法
DK3095861T3 (da) * 2010-05-06 2019-09-23 Procter & Gamble Forbrugerprodukter med proteasevarianter
AU2012260576B2 (en) 2011-05-20 2015-07-09 Ecolab Usa Inc. Acid formulations for use in a system for warewashing
BR112013028007A2 (pt) 2011-05-20 2017-01-10 Ecolab Usa Inc detergentes não-fosfato e ácidos não-fosfóricos em um sistema alternado alcalinidade/acidez para a lavagem de louça
AU2012277729B2 (en) * 2011-06-30 2016-12-08 Novozymes A/S Method for screening alpha-amylases
ES2750872T3 (es) 2011-12-13 2020-03-27 Ecolab Usa Inc Método de lavado de vajilla
CN107267489A (zh) 2011-12-20 2017-10-20 诺维信公司 枯草杆菌酶变体和编码它们的多核苷酸
EP2607468A1 (en) 2011-12-20 2013-06-26 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
WO2013120948A1 (en) * 2012-02-17 2013-08-22 Novozymes A/S Subtilisin variants and polynucleotides encoding same
US9745543B2 (en) 2012-09-10 2017-08-29 Ecolab Usa Inc. Stable liquid manual dishwashing compositions containing enzymes
ES2743376T3 (es) 2013-09-09 2020-02-18 Ecolab Usa Inc Eliminación sinérgica de manchas mediante una nueva combinación de agentes quelantes
CA2921811A1 (en) 2013-09-09 2015-03-12 Ecolab Usa Inc. Synergistic stain removal through chelator combination
CN105683350B (zh) 2013-10-24 2019-03-05 艺康美国股份有限公司 用于从表面上除去污物的组合物和方法
DE102016204814A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen
DE102016204815A1 (de) * 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Proteasen mit verbesserte Enzymstabilität in Waschmittel
US20210009979A1 (en) 2018-03-23 2021-01-14 Novozymes A/S Subtilase variants and compositions comprising same
WO2021022045A1 (en) 2019-07-31 2021-02-04 Ecolab Usa Inc. Personal protective equipment free delimer compositions

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US5801038A (en) * 1984-05-29 1998-09-01 Genencor International Inc. Modified subtilisins having amino acid alterations
US5182204A (en) * 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US5371008A (en) * 1984-05-29 1994-12-06 Genencor International, Inc. Substrate assisted catalysis
US5346823A (en) * 1984-05-29 1994-09-13 Genencor, Inc. Subtilisin modifications to enhance oxidative stability
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5244791A (en) * 1984-05-29 1993-09-14 Genecor International, Inc. Methods of ester hydrolysis
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5371190A (en) * 1984-05-29 1994-12-06 Genencor International, Inc. Substrate assisted catalysis
JP2599946B2 (ja) 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド ズブチリシン類似体
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
EP0260299A4 (en) 1986-02-12 1988-11-24 Genex Corp MUTAGENESIS AND SCREENING PROCESS AND PRODUCT OBTAINED.
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5013657A (en) 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US5240632A (en) * 1986-03-26 1993-08-31 Amway Corporation Machine dishwasher water spot control composition
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
AU620026B2 (en) * 1987-02-27 1992-02-13 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
JP3155984B2 (ja) * 1987-04-06 2001-04-16 ノヴォザイムズ・アクティーゼルスカブ タンパク質の安定化のための金属イオン結合部位における静電的相互作用の操作
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
AU595096B2 (en) * 1988-02-10 1990-03-22 Astra Pharmaceuticals Pty Ltd Plastic cartridge and syringe
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
PT89702B (pt) 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5116741A (en) 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5246849A (en) * 1988-04-12 1993-09-21 Enzon, Inc. Thermally stable serine proteases
US5118623A (en) * 1988-05-27 1992-06-02 Solvay Enzymes, Inc. Bleach stable enzymes
JPH03505976A (ja) 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
JPH04500384A (ja) 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
DK316989D0 (da) 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
BR9006827A (pt) 1989-06-26 1991-08-06 Unilever Nv Composicoes detergentes enzimaticas
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DE4009534A1 (de) * 1990-03-24 1991-09-26 Henkel Kgaa Fluessiges handreinigungsmittel
DK97190D0 (da) * 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
JP3362847B2 (ja) * 1990-08-09 2003-01-07 ジェネンテック インコーポレイテッド ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
DK0563169T3 (da) 1990-12-21 1998-05-18 Novo Nordisk As Enzymmutanter med lille variation i molekylær ladning i et pH område
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
EP0525610A3 (en) * 1991-07-27 1993-03-24 Solvay Enzymes Gmbh & Co. Kg Process for increasing the stability of enzymes and stabilized enzymes
US5275945A (en) * 1991-10-08 1994-01-04 Vista Chemical Company Alkaline proteases stable in heavy-duty detergent liquids
AU682314B2 (en) 1992-07-17 1997-10-02 Genencor International, Inc. High alkaline serine proteases
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
DE69434873T2 (de) 1993-09-15 2007-09-06 The Procter & Gamble Company, Cincinnati Subtilisin bpn'-varianten mit verminderter adsorption und erhöhter hydrolyse
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
EP0723580B1 (en) * 1993-10-14 2003-07-16 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
JP2888985B2 (ja) * 1993-10-14 1999-05-10 ザ、プロクター、エンド、ギャンブル、カンパニー プロテアーゼ含有クリーニング組成物
ES2364774T3 (es) * 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues

Also Published As

Publication number Publication date
WO1995030010A1 (en) 1995-11-09
CZ321696A3 (en) 1997-05-14
HU9603031D0 (en) 1997-01-28
CA2189426A1 (en) 1995-11-09
JPH09511652A (ja) 1997-11-25
AU2159195A (en) 1995-11-29
BR9507597A (pt) 1997-10-07
PE27897A1 (es) 1997-08-13
US6436690B1 (en) 2002-08-20
CA2189426C (en) 2002-09-17
CN1151761A (zh) 1997-06-11
IL113048A0 (en) 1995-06-29
EP0758388A1 (en) 1997-02-19
HUT75559A (en) 1997-05-28

Similar Documents

Publication Publication Date Title
MX9605357A (es) Variantes de subtilisina bpn' que tiene absorcion reducida e hidrolisis incrementada.
MX9605358A (es) Variantes de subtilicina 309 con absorcion disminuida e hidrolisis aumentada.
MX9706851A (es) Variantes de subtilisina carlseberg que tienen adsorcion reducida e hidrolisis incrementada.
MX9605359A (es) Composiciones para la limpieza de telas que contienen variantes de subtilicina bpn.
MX9706841A (es) Variantes de proteinasa k que tienen adsorcion reducida e hidrolisis incrementada.
HU9600667D0 (en) Subtilisin bpn' variants with decreased adsorption and increased hydrolysis
MX9706847A (es) Variantes de subtilisina dy que tienen adsorcion reducida e hidrolisis incrementada.
AU6424394A (en) Protease variants
HU9600960D0 (en) Protease-containing cleaning compositions
DE69432954D1 (de) Proteasen enthaltende bleichmittelzusammensetzungen
DK0728148T3 (da) Forbedrede immunogene sammensætninger mod humant gastrin 17
PH30705A (en) Detergent composition comprising an alkyl sulphateand a subtilisin variant.
PT975761E (pt) Interferao beta humano recombinante com solubilidade melhorada
WO1999005291A3 (en) Aggrecan degrading metallo proteases
MX9706846A (es) Variantes de termitasa que tienen adsorcion reducida e hidrolisis incrementada.
WO2000001831A3 (en) Proteases fused with variants of streptomyces subtilisin inhibitor
WO2000001826A3 (en) Stabilized variants of streptomyces subtilisin inhibitor
AU7655994A (en) Cloning of a member of the serine-threonine-kinase family
EP0945512A3 (en) Novel serine proteases
EP1158052A4 (en) BETA-PRIMEVEROSIDASDE GENE
TH20907A (th) Bpn' แวเรียนท์ที่มีการดูดซับที่ลดลงและเพิ่มการไฮโดรไลซิสโดยไลซิสโดยที่มีการแทนที่บริเวณลูปหนึ่งแห่งหรือมากกว่า