MX384887B - Ensamblaje de adn mediado por nucleasa. - Google Patents
Ensamblaje de adn mediado por nucleasa.Info
- Publication number
- MX384887B MX384887B MX2021000857A MX2021000857A MX384887B MX 384887 B MX384887 B MX 384887B MX 2021000857 A MX2021000857 A MX 2021000857A MX 2021000857 A MX2021000857 A MX 2021000857A MX 384887 B MX384887 B MX 384887B
- Authority
- MX
- Mexico
- Prior art keywords
- sequences
- create
- overlapping
- complementary
- nuclease
- Prior art date
Links
- 230000000295 complement effect Effects 0.000 abstract 3
- 101710163270 Nuclease Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 230000005782 double-strand break Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462015809P | 2014-06-23 | 2014-06-23 | |
| US201462016400P | 2014-06-24 | 2014-06-24 | |
| US201462036983P | 2014-08-13 | 2014-08-13 | |
| PCT/US2015/037199 WO2015200334A1 (en) | 2014-06-23 | 2015-06-23 | Nuclease-mediated dna assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2021000857A MX2021000857A (es) | 2021-07-28 |
| MX384887B true MX384887B (es) | 2025-03-14 |
Family
ID=53525285
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016016905A MX379237B (es) | 2014-06-23 | 2015-06-23 | Ensamblaje de adn mediado por nucleasa. |
| MX2021000857A MX384887B (es) | 2014-06-23 | 2015-06-23 | Ensamblaje de adn mediado por nucleasa. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016016905A MX379237B (es) | 2014-06-23 | 2015-06-23 | Ensamblaje de adn mediado por nucleasa. |
Country Status (25)
| Country | Link |
|---|---|
| US (6) | US9738897B2 (enExample) |
| EP (3) | EP3155099B1 (enExample) |
| JP (4) | JP6336140B2 (enExample) |
| KR (2) | KR101822902B1 (enExample) |
| CN (2) | CN112029787B (enExample) |
| AU (3) | AU2015280120B2 (enExample) |
| BR (1) | BR112016030145A8 (enExample) |
| CA (1) | CA2953499C (enExample) |
| CY (2) | CY1120520T1 (enExample) |
| DK (2) | DK3354732T3 (enExample) |
| ES (2) | ES2781323T3 (enExample) |
| HR (1) | HRP20200523T1 (enExample) |
| HU (1) | HUE049405T2 (enExample) |
| IL (1) | IL249612B (enExample) |
| LT (1) | LT3354732T (enExample) |
| MX (2) | MX379237B (enExample) |
| NZ (1) | NZ765591A (enExample) |
| PL (2) | PL3155099T3 (enExample) |
| PT (2) | PT3155099T (enExample) |
| RS (1) | RS60366B1 (enExample) |
| RU (1) | RU2707911C2 (enExample) |
| SG (2) | SG10201803444YA (enExample) |
| SI (1) | SI3354732T1 (enExample) |
| SM (1) | SMT202000174T1 (enExample) |
| WO (1) | WO2015200334A1 (enExample) |
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| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
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| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
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