MX362523B - Direccion transmitida por nucleasa con vectores de direccion grandes. - Google Patents
Direccion transmitida por nucleasa con vectores de direccion grandes.Info
- Publication number
- MX362523B MX362523B MX2014012994A MX2014012994A MX362523B MX 362523 B MX362523 B MX 362523B MX 2014012994 A MX2014012994 A MX 2014012994A MX 2014012994 A MX2014012994 A MX 2014012994A MX 362523 B MX362523 B MX 362523B
- Authority
- MX
- Mexico
- Prior art keywords
- targeting
- nuclease
- genomic locus
- target genomic
- mediated
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Abstract
La presente invención se refiere a composiciones y métodos para elaborar una o más modificaciones genéticas dirigidas en un locus genómico objetivo empleando recombinación homóloga facilitada por el rompimiento de hebra simple o doble en o cerca del locus genómico objetivo. También se proporcionan composiciones y métodos para promover la eficiencia de recombinación homóloga entre un LTVEC y un locus genómico objetivo en células procarióticas o eucarióticas o utilizando nucleasas diseñadas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261638267P | 2012-04-25 | 2012-04-25 | |
PCT/US2013/038165 WO2013163394A1 (en) | 2012-04-25 | 2013-04-25 | Nuclease-mediated targeting with large targeting vectors |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2014012994A MX2014012994A (es) | 2015-02-04 |
MX362523B true MX362523B (es) | 2019-01-22 |
Family
ID=48289700
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2014012994A MX362523B (es) | 2012-04-25 | 2013-04-25 | Direccion transmitida por nucleasa con vectores de direccion grandes. |
Country Status (20)
Country | Link |
---|---|
US (2) | US9834786B2 (es) |
EP (1) | EP2847335B1 (es) |
JP (1) | JP6275120B2 (es) |
KR (1) | KR101904508B1 (es) |
CN (2) | CN104364380B (es) |
AU (1) | AU2013251558B2 (es) |
BR (1) | BR112014026294B1 (es) |
CA (1) | CA2869016C (es) |
CY (1) | CY1120572T1 (es) |
DK (1) | DK2847335T3 (es) |
ES (1) | ES2683071T3 (es) |
HK (1) | HK1207396A1 (es) |
IL (1) | IL234905B (es) |
IN (1) | IN2014DN09261A (es) |
MX (1) | MX362523B (es) |
PL (1) | PL2847335T3 (es) |
PT (1) | PT2847335T (es) |
RU (1) | RU2645475C2 (es) |
SG (2) | SG10201702445TA (es) |
WO (1) | WO2013163394A1 (es) |
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