MX354845B - Aparato y proceso de purificación de proteínas. - Google Patents

Aparato y proceso de purificación de proteínas.

Info

Publication number
MX354845B
MX354845B MX2012013411A MX2012013411A MX354845B MX 354845 B MX354845 B MX 354845B MX 2012013411 A MX2012013411 A MX 2012013411A MX 2012013411 A MX2012013411 A MX 2012013411A MX 354845 B MX354845 B MX 354845B
Authority
MX
Mexico
Prior art keywords
chromatography resin
mixed
depth filter
capture
protein
Prior art date
Application number
MX2012013411A
Other languages
English (en)
Other versions
MX2012013411A (es
Inventor
K Hickman Robert
Wang Chen
O Lundell Edwin
D Hegedus Roy
Original Assignee
Abbvie Inc Star
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbvie Inc Star filed Critical Abbvie Inc Star
Publication of MX2012013411A publication Critical patent/MX2012013411A/es
Publication of MX354845B publication Critical patent/MX354845B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a un método para purificar una proteína terapéutica, que comprende los pasos de: a. proporcionar una muestra que contiene la proteína; b. procesar la muestra a través de un primer paso de cromatografía por afinidad que comprende una resina de cromatografía de captura por afinidad contenida dentro de una columna de captura para proporcionar una primer eluato que comprende la proteína; c. después de que la muestra se procesa a través de la resina de cromatografía de captura por afinidad, procesar el primer eluato a través de un filtro profundo para proporcionar un eluato filtrado que comprende la proteína, en el cual el filtro profundo tiene tamaños de poro de 0.01 µm a 8 µm; y d. después de que el primer eluato se procesa a través del filtro profundo, procesar el eluato filtrado a través de un segundo paso de cromatografía que comprende una resina de cromatografía de modo mezclado contenida dentro de una columna de modo mezclado para proporcionar un segundo eluato que comprende la proteína, en donde procesar a través del primer y segundo pasos de cromatografía son los únicos pasos de cromatografía del método.
MX2012013411A 2010-05-18 2011-04-13 Aparato y proceso de purificación de proteínas. MX354845B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34563410P 2010-05-18 2010-05-18
PCT/US2011/032279 WO2011146179A2 (en) 2010-05-18 2011-04-13 Apparatus and process of purification of proteins

Publications (2)

Publication Number Publication Date
MX2012013411A MX2012013411A (es) 2013-06-28
MX354845B true MX354845B (es) 2018-03-22

Family

ID=44305048

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012013411A MX354845B (es) 2010-05-18 2011-04-13 Aparato y proceso de purificación de proteínas.

Country Status (16)

Country Link
US (2) US20110301342A1 (es)
EP (1) EP2571892A2 (es)
JP (2) JP6039550B2 (es)
KR (1) KR20130086540A (es)
CN (1) CN103038247A (es)
AU (1) AU2011256727B2 (es)
BR (1) BR112012030530A2 (es)
CA (1) CA2799502A1 (es)
IL (1) IL223073B (es)
MX (1) MX354845B (es)
NZ (1) NZ603616A (es)
RU (1) RU2012154652A (es)
SG (2) SG185601A1 (es)
TW (1) TW201204742A (es)
WO (1) WO2011146179A2 (es)
ZA (1) ZA201209048B (es)

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JP6268167B2 (ja) * 2012-05-31 2018-01-24 エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ タンパク質製剤中の凝集物含量を低減するために混合型多官能表面を使用する方法
CN103159824B (zh) * 2013-02-06 2015-11-25 中国科学院生物物理研究所 一种全封闭管路化的蛋白质纯化系统及其在无菌无热原蛋白质药物制备上的应用
AU2014223583B2 (en) * 2013-02-26 2019-03-28 Merck Millipore Ltd. Mixed-mode chromatography membranes
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014145744A1 (en) * 2013-03-15 2014-09-18 Alder Biopharmaceuticals, Inc. Antibody purification and purity monitoring
EP3057616B1 (en) 2013-10-16 2020-03-11 Outlook Therapeutics, Inc. Buffer formulations for enhanced antibody stability
US20160272673A1 (en) * 2013-11-07 2016-09-22 Abbvie Inc. Isolation and purification of dvd-igs
EP3099799B1 (en) * 2014-01-28 2019-05-15 Dice Molecules SV. LLC Arrays of monoliths with attached recognition compounds
JP6783767B2 (ja) * 2014-12-22 2020-11-11 アレクシオン ファーマシューティカルズ インコーポレイテッドＡｌｅｘｉｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 組換えタンパク質を精製する方法
EP3247718B1 (en) 2015-01-21 2021-09-01 Outlook Therapeutics, Inc. Modulation of charge variants in a monoclonal antibody composition
CN106317166A (zh) * 2015-07-02 2017-01-11 北京中原领先科技有限公司 高效过滤层析混合机制分离蛋白装置
JP2017025025A (ja) * 2015-07-23 2017-02-02 旭化成メディカル株式会社 タンパク質の精製方法
EP3334747B1 (en) 2015-08-13 2023-09-27 Amgen Inc. Charged depth filtration of antigen-binding proteins
JP7032046B2 (ja) * 2016-01-22 2022-03-08 旭化成メディカル株式会社 生理活性物質の連続的な定流速精製方法
CN109563161A (zh) 2016-02-03 2019-04-02 安口生物公司 用于提高抗体稳定性的缓冲制剂
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CN108059650A (zh) * 2018-01-05 2018-05-22 上海药明生物技术有限公司 低pH病毒灭活工艺中的纯化方法
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
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KR102337683B1 (ko) * 2018-09-21 2021-12-13 주식회사 녹십자 고효율 항-tfpi 항체 조성물
CN109369776B (zh) * 2018-11-09 2020-12-25 杭州奕安济世生物药业有限公司 一种蛋白纯化系统及方法
US11504716B2 (en) * 2020-06-05 2022-11-22 Pall Corporation Multiwell device and method of use
CN112979745A (zh) * 2021-04-29 2021-06-18 江西天佳生物工程股份有限公司 一种固相萃取提纯抗菌肽的方法
CN113926434A (zh) * 2021-11-03 2022-01-14 上海江夏血液技术有限公司 一种蛋白吸附膜材料及其制备方法和应用

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Also Published As

Publication number Publication date
IL223073A0 (en) 2013-02-03
CA2799502A1 (en) 2011-11-24
EP2571892A2 (en) 2013-03-27
AU2011256727A1 (en) 2012-12-06
US20110301342A1 (en) 2011-12-08
NZ603616A (en) 2015-02-27
WO2011146179A3 (en) 2012-01-19
IL223073B (en) 2018-01-31
CN103038247A (zh) 2013-04-10
SG185601A1 (en) 2012-12-28
AU2011256727B2 (en) 2015-08-13
TW201204742A (en) 2012-02-01
KR20130086540A (ko) 2013-08-02
RU2012154652A (ru) 2014-06-27
SG10201503899SA (en) 2015-06-29
JP6039550B2 (ja) 2016-12-07
JP2013531623A (ja) 2013-08-08
BR112012030530A2 (pt) 2022-12-20
MX2012013411A (es) 2013-06-28
JP2017002049A (ja) 2017-01-05
US20150065696A1 (en) 2015-03-05
WO2011146179A2 (en) 2011-11-24
ZA201209048B (en) 2013-08-28

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