MX2022014399A - Ensayo basado en crispr para la deteccion de patogenos en muestras. - Google Patents

Ensayo basado en crispr para la deteccion de patogenos en muestras.

Info

Publication number
MX2022014399A
MX2022014399A MX2022014399A MX2022014399A MX2022014399A MX 2022014399 A MX2022014399 A MX 2022014399A MX 2022014399 A MX2022014399 A MX 2022014399A MX 2022014399 A MX2022014399 A MX 2022014399A MX 2022014399 A MX2022014399 A MX 2022014399A
Authority
MX
Mexico
Prior art keywords
crispr
samples
based assay
sample
guide rna
Prior art date
Application number
MX2022014399A
Other languages
English (en)
Inventor
Ye Tony Hu
Bo Ning
Zhen Huang
Original Assignee
The Administrators Of The Tulane Educational Fund
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Administrators Of The Tulane Educational Fund filed Critical The Administrators Of The Tulane Educational Fund
Publication of MX2022014399A publication Critical patent/MX2022014399A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención describe un método para detectar la presencia de patógenos, incluido el SARS-CoV-2, en una muestra. El método usa proteínas efectoras de CRISPR junto con un ARN guía y una molécula indicadora. Las moléculas de ARN de la muestra primero se extraen opcionalmente y se transcriben inversamente, seguidas de la amplificación, de tal manera que cuando el ARN guía se hibrida con un fragmento de nucleótido objetivo en el ADN amplificado, la proteína efectora de CRISPR escinde la molécula indicadora, lo que da como resultado una señal detectable.
MX2022014399A 2020-05-20 2021-05-20 Ensayo basado en crispr para la deteccion de patogenos en muestras. MX2022014399A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063027530P 2020-05-20 2020-05-20
PCT/US2021/033497 WO2021236987A2 (en) 2020-05-20 2021-05-20 Crispr-based assay for detecting pathogens in samples

Publications (1)

Publication Number Publication Date
MX2022014399A true MX2022014399A (es) 2023-01-04

Family

ID=78707694

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022014399A MX2022014399A (es) 2020-05-20 2021-05-20 Ensayo basado en crispr para la deteccion de patogenos en muestras.

Country Status (7)

Country Link
US (1) US20230183817A1 (es)
EP (1) EP4153309A2 (es)
JP (1) JP2023527145A (es)
CN (1) CN116438299A (es)
BR (1) BR112022023512A2 (es)
MX (1) MX2022014399A (es)
WO (1) WO2021236987A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114395636B (zh) * 2022-02-11 2024-04-23 南方医科大学 一种基于RPA-CRISPR/Cas12a的人型支原体检测系统及其应用
CN114717363A (zh) * 2022-06-09 2022-07-08 中国科学院深圳先进技术研究院 一种病原突变体的即时核酸检测方法及检测试剂盒
KR20240020320A (ko) * 2022-08-04 2024-02-15 한국생명공학연구원 SARS-CoV-2 RdRp 변이 육안 검출법
CN115807130A (zh) * 2022-11-16 2023-03-17 武汉大学 猴痘病毒快速检测方法
CN115651807B (zh) * 2022-11-18 2023-05-09 北京理工大学 核酸检测芯片和核酸检测方法
CN116479175A (zh) * 2023-03-13 2023-07-25 广州国家实验室 用于检测人副流感病毒核酸的检测系统、试剂盒及方法
CN117467804B (zh) * 2023-12-26 2024-04-02 丹娜(天津)生物科技股份有限公司 一种用于呼吸道合胞病毒核酸快速检测的引物及其应用
CN117604168A (zh) * 2024-01-19 2024-02-27 南京鸿明生物科技有限公司 一种免扩增的rna病毒基因组滴度测量方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070083945A1 (en) * 2000-03-10 2007-04-12 Byrum Joseph R Nucleic acid molecules and other molecules associated with plants
US7339051B2 (en) * 2003-04-28 2008-03-04 Isis Pharmaceuticals, Inc. Compositions and methods for the treatment of severe acute respiratory syndrome (SARS)
WO2017025984A1 (en) * 2015-08-07 2017-02-16 Council Of Scientific And Industrial Research Smartphone integrated real - time molecular diagnostic device
IL291971A (en) * 2016-03-04 2022-06-01 Editas Medicine Inc Methods related to crispr-cpf1, compositions and components for cancer immunotherapy

Also Published As

Publication number Publication date
BR112022023512A2 (pt) 2022-12-20
CN116438299A (zh) 2023-07-14
EP4153309A2 (en) 2023-03-29
WO2021236987A3 (en) 2021-12-23
US20230183817A1 (en) 2023-06-15
WO2021236987A2 (en) 2021-11-25
JP2023527145A (ja) 2023-06-27

Similar Documents

Publication Publication Date Title
MX2022014399A (es) Ensayo basado en crispr para la deteccion de patogenos en muestras.
CN110684823A (zh) 一种基于试纸条的Cas12a酶的微生物快速诊断技术
AU7125401A (en) Materials and methods for detection of nucleic acids
US10465236B2 (en) Nucleic acid detection method and kit
CA2692633A1 (en) Method for the simultaneous detection of multiple nucleic acid sequences in a sample
CN107988340B (zh) 一种快速检测绵羊肺炎支原体的pcr扩增引物及其应用
MX2022009614A (es) Ensayo a base de crispr para la deteccion de tb en fluidos corporales.
CN110923314B (zh) 一组检测SNP位点rs9263726的引物、crRNA序列及其应用
RU2013153505A (ru) Способы, системы и композиции для детекции микробной днк при помощи пцр
US20210139968A1 (en) Rna amplification method, rna detection method and assay kit
US8188257B1 (en) Direct quantification of ribosome inactivating protein
JP2020533974A5 (es)
Metcalf et al. Evaluation of commercial kits for extraction of DNA and RNA from Clostridium difficile
BR0014518A (pt) Método e ácidos nucléicos para a detecção de microorganismos pertinentes à fermentação
CN104946637B (zh) 一种向日葵黄萎病的两种轮枝病菌的多重dpo‑pcr检测试剂盒及其应用
CN110564822B (zh) 基于LAMP技术的转基因玉米Bt176相关基因检测方法及试剂盒
WO2010124281A3 (en) Selective detection of bordetella species
Rodriguez et al. Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA
CN109266768A (zh) 一种用于鉴别近缘微生物的核苷酸片段的筛选方法
WO2012048339A3 (en) Selective detection of neisseria meningitidis
CN108796090A (zh) 一种检测食品中羊源成分的试剂盒以及检测方法
CN115896322A (zh) 一种基于重组酶聚合酶扩增方法检测稻曲病菌的引物、探针和试剂盒
Shi et al. Isothermal amplification detection of nucleic acids by a double-nicked beacon
JP4788942B2 (ja) 特定生物種検出のためのデオキシリボザイム
CN108103153B (zh) 一种小麦白粉菌的lamp检测引物组合及其lamp检测试剂盒和lamp方法