BR0014518A - Método e ácidos nucléicos para a detecção de microorganismos pertinentes à fermentação - Google Patents

Método e ácidos nucléicos para a detecção de microorganismos pertinentes à fermentação

Info

Publication number
BR0014518A
BR0014518A BR0014518-1A BR0014518A BR0014518A BR 0014518 A BR0014518 A BR 0014518A BR 0014518 A BR0014518 A BR 0014518A BR 0014518 A BR0014518 A BR 0014518A
Authority
BR
Brazil
Prior art keywords
nucleic acid
detection
brevis
fermentation
nucleic acids
Prior art date
Application number
BR0014518-1A
Other languages
English (en)
Inventor
Markus Fandke
Alexander Gasch
Kornelia Berghof
Original Assignee
Biotecon Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotecon Diagnostics Gmbh filed Critical Biotecon Diagnostics Gmbh
Publication of BR0014518A publication Critical patent/BR0014518A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Patente de Invenção: "MéTODO E áCIDOS NUCLéICOS PARA A DETECçãO DE MICROORGANISMOS PERTINENTES A FERMENTAçãO". A invenção refere-se à um método para a detecção de microorganismo pertinente à fermentação, assim como à ácidos nucléicos e combinações destes os quais podem ser empregados neste método. A invenção também refere-se ao emprego dos ácidos nucléicos de acordo com a invenção ou combinação para detecção e/ou para a identificação e /ou caracterização de diferentes gêneros ou espécies de microorganismos pertinentes à fermentação. Em conseq³ência, o problema a ser resolvido pela presente invenção era fornecer um método e recursos que tornem possível um teste rápido de cerveja e materiais brutos de fermentação por contaminação com microorganismos, o teste sendo requerido para detectar a faixa total de possíveis microorganismos de contaminação de cerveja. Este problema é resolVido de acordo com a invenção por um método que compreende as seguintes etapas: (a) trazer a amostra em contato com uma combinação de pelo menos duas primeiras moléculas de ácido nucléico (iniciadores), que hibridizam com uma região de um ácido nucléico microbiano conservado em microorganismos pertinentes à fermentação; (b) amplificação do ácido nucléico microbiano ou uma porção deste para produzir pelo menos um fragmento de amplificação; (c) trazer os fragmentos de amplificação obtido na etapa (b) em contato com pelo menos uma segunda molécula de ácido nucléico (sonda) que especificamente hibridiza com pelo menos um fragmento de amplificação o qual compreende uma seq³ência do ácido nucléico microbiano específico para todos os microorganismos pertinentes à fermentação ou para uma ou diversas famílias, gêneros ou espécies de microorganismos pertinentes à fermentação; e (d) detectar pelo menos um ácido nucléico híbrido que consista em um fragmento de amplificação e uma segunda molécula de ácido nucléico introduzida na etapa (c). Além disso são fornecidos ácidos nucléicos que podem ser empregados no método de acordo com a invenção.
BR0014518-1A 1999-09-24 2000-09-08 Método e ácidos nucléicos para a detecção de microorganismos pertinentes à fermentação BR0014518A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19945964A DE19945964A1 (de) 1999-09-24 1999-09-24 Verfahren und Nukleinsäuren zum Nachweis von brauereirelevanten Mikroorganismen
PCT/EP2000/008808 WO2001023605A2 (de) 1999-09-24 2000-09-08 Verfahren und nukleinsäuren zum nachweis von brauereirelevanten mikroorganismen

Publications (1)

Publication Number Publication Date
BR0014518A true BR0014518A (pt) 2002-06-11

Family

ID=7923267

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014518-1A BR0014518A (pt) 1999-09-24 2000-09-08 Método e ácidos nucléicos para a detecção de microorganismos pertinentes à fermentação

Country Status (11)

Country Link
US (1) US7183085B1 (pt)
EP (1) EP1214450B1 (pt)
JP (1) JP2003510091A (pt)
CN (1) CN100569952C (pt)
AT (1) ATE335849T1 (pt)
AU (1) AU781702C (pt)
BR (1) BR0014518A (pt)
CA (1) CA2385652C (pt)
DE (2) DE19945964A1 (pt)
MX (1) MXPA02003134A (pt)
WO (1) WO2001023605A2 (pt)

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DE10129410A1 (de) * 2001-06-19 2003-01-02 Vermicon Ag Verfahren zum spezifischen Schnellnachweis bierschädlicher Bakterien
KR101151493B1 (ko) * 2004-02-25 2012-05-30 산토리 홀딩스 가부시키가이샤 세균 검출 기구, 세균 검출 방법 및 세균 검출 키트
US9303281B2 (en) 2012-07-23 2016-04-05 Pall Corporation Compositions for detecting foodstuff spoilage microorganisms
CN103397092B (zh) * 2013-07-30 2015-02-04 青岛啤酒股份有限公司 一种啤酒污染菌的快速筛查方法
CN103361439B (zh) * 2013-07-30 2014-11-26 青岛啤酒股份有限公司 一种集成检测9种啤酒污染菌的方法
CN105603081B (zh) * 2016-01-29 2019-12-06 北京工商大学 一种非诊断目的的肠道微生物定性与定量的检测方法
CN105740650B (zh) * 2016-03-02 2019-04-05 广西作物遗传改良生物技术重点开放实验室 一种快速准确鉴定高通量基因组数据污染源的方法
KR101919190B1 (ko) * 2016-04-27 2018-11-15 하이트진로 주식회사 미생물의 ２ 단계 분석 방법
CN113077845A (zh) * 2021-04-13 2021-07-06 中国科学院大气物理研究所 一种大气气溶胶微生物群落组成的分析方法

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Also Published As

Publication number Publication date
EP1214450B1 (de) 2006-08-09
CA2385652A1 (en) 2001-04-05
WO2001023605A3 (de) 2002-02-21
CN100569952C (zh) 2009-12-16
AU7285900A (en) 2001-04-30
CN1376206A (zh) 2002-10-23
DE19945964A1 (de) 2001-04-05
MXPA02003134A (es) 2003-08-20
JP2003510091A (ja) 2003-03-18
CA2385652C (en) 2011-11-15
AU781702B2 (en) 2005-06-09
ATE335849T1 (de) 2006-09-15
US7183085B1 (en) 2007-02-27
DE50013309D1 (de) 2006-09-21
AU781702C (en) 2006-04-06
EP1214450A2 (de) 2002-06-19
WO2001023605A2 (de) 2001-04-05

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Legal Events

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O C/C 13, 24, 25 E 10 (IX) DA LPI.

B09T Decision of refusal: decision cancelled [chapter 9.2.1 patent gazette]

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B09B Patent application refused [chapter 9.2 patent gazette]

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B09B Patent application refused [chapter 9.2 patent gazette]

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