MX2022007905A - Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos. - Google Patents

Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos.

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Publication number
MX2022007905A
MX2022007905A MX2022007905A MX2022007905A MX2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A
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MX
Mexico
Prior art keywords
temperature
cells
subject
sensitive
methods
Prior art date
Application number
MX2022007905A
Other languages
English (en)
Spanish (es)
Inventor
Minoru S H Ko
Original Assignee
Elixirgen Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elixirgen Therapeutics Inc filed Critical Elixirgen Therapeutics Inc
Publication of MX2022007905A publication Critical patent/MX2022007905A/es

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Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016341309A1 (en) 2015-10-22 2018-06-07 Modernatx, Inc. Cancer vaccines
MX2022007905A (es) 2019-12-31 2022-08-25 Elixirgen Therapeutics Inc Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos.
TWI891771B (zh) 2020-04-17 2025-08-01 美商Ｖｌｐ醫療股份有限公司 冠狀病毒疫苗
IL297419B2 (en) 2020-04-22 2025-02-01 BioNTech SE Coronavirus vaccine
KR20240023600A (ko) * 2021-06-17 2024-02-22 엘릭서젠 쎄라퓨틱스, 인크. 바이러스 질환을 위한 온도-제어가능한, 자기-복제 rna 백신
WO2023034881A1 (en) * 2021-09-02 2023-03-09 Elixirgen Therapeutics, Inc. Temperature-controllable, rna immunotherapeutic for cancer
US12186387B2 (en) 2021-11-29 2025-01-07 BioNTech SE Coronavirus vaccine
GB2631601A (en) * 2022-02-04 2025-01-08 Elixirgen Llc Cell therapy protocol systems and methods
WO2024002985A1 (en) 2022-06-26 2024-01-04 BioNTech SE Coronavirus vaccine
EP4622664A1 (en) 2022-11-22 2025-10-01 Elixirgen Therapeutics, Inc. Antigens for cancer immunotherapy

Family Cites Families (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5217879A (en) 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
WO1996031776A1 (en) 1995-04-06 1996-10-10 Miltenyi Bioteh, Inc. Multiparameter cell separation using releasable colloidal magnetic particles
US6451592B1 (en) 1996-04-05 2002-09-17 Chiron Corporation Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
US6458560B1 (en) 1996-04-05 2002-10-01 Chiron Corporation Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
JP2002509729A (ja) * 1998-03-27 2002-04-02 サイトス バイオテクノロジー アーゲー 誘導性のアルファウイルス遺伝子発現系
AU4932199A (en) 1998-07-31 2000-02-21 Haruo Sugiyama Cancer antigens based on tumor suppressor gene wt1 product
US8647864B2 (en) 1999-04-14 2014-02-11 Novartis Ag Compositions and methods for generating an immune response utilizing alphavirus-based vector systems
DE60044125D1 (de) 1999-04-14 2010-05-20 Novartis Vaccines & Diagnostic Zusammensetzungen und verfahren zur auslösung einer immunantwort auf basis alphavirus-vektoren-systeme
ES2288473T3 (es) 1999-05-18 2008-01-16 Dnavec Research Inc. Vector de virus deficiente en el gen de la envoltura de paramyxoviridae.
JP4608210B2 (ja) 2001-05-31 2011-01-12 ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド キメラアルファウイルスレプリコン粒子
US20030232324A1 (en) 2001-05-31 2003-12-18 Chiron Corporation Chimeric alphavirus replicon particles
ES2330202T5 (es) 2001-09-06 2014-01-20 Alphavax, Inc. Sistemas vectores basados en replicones de alfavirus
MXPA05001644A (es) 2002-08-13 2005-04-19 Akzo Nobel Nv Replicones de pestivirus que no expresan proteina c y/o e1, y particulas virales infecciosas que los contienen y pueden usarse en vacunas.
CA2499404A1 (en) * 2002-09-18 2004-04-01 The United States Of America As Represented By The Secretary Of The Department Of Health & Human Services Recovery of recombinant human parainfluenza virus type 2 (hpiv2) from cdna and use of recombinant hpiv2 in immunogenic compositions and as vectors to elicit immune responses against piv and other human pathogens
WO2004055161A2 (en) 2002-12-12 2004-07-01 The Board Of Regents Of The University Of Texas System Large scale production of packaged alphavirus replicons
US7078218B2 (en) 2002-12-13 2006-07-18 Alphavax, Inc. Alphavirus particles and methods for preparation
US7442381B2 (en) 2003-03-20 2008-10-28 Alphavax, Inc. Alphavirus replicons and helper constructs
US20050282279A1 (en) * 2003-05-29 2005-12-22 Hwu Paul L Expression vector encoding coronavirus-like particle
WO2004108937A1 (en) * 2003-06-04 2004-12-16 Bioleaders Corporation Cell surface expression vector of sars virus antigen and microorganisms transformed thereby
KR20060017635A (ko) 2003-06-05 2006-02-24 와이어쓰 홀딩스 코포레이션 베네쥬엘라 말 뇌염 바이러스 레플리콘 벡터 및파라믹소바이러스 단백질 항원을 포함하는 면역원성 조성물
CN1989250B (zh) 2004-05-18 2013-11-20 阿尔法瓦克斯公司 Tc-83衍生的甲病毒属载体、颗粒和方法
US7332322B2 (en) 2004-09-14 2008-02-19 Ilya Frolov Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
US7998733B2 (en) * 2004-10-05 2011-08-16 Merial Limited Chimeric vectors
JP4478788B2 (ja) 2005-05-27 2010-06-09 独立行政法人産業技術総合研究所 センダイウイルス温度感受性株由来のウイルスベクター
PL2578685T3 (pl) 2005-08-23 2020-01-31 The Trustees Of The University Of Pennsylvania Rna zawierający zmodyfikowane nukleozydy i sposoby jego zastosowania
EP1951281B1 (en) 2005-10-17 2015-04-15 Sloan Kettering Institute For Cancer Research Wt1 hla class ii-binding peptides and compositions and methods comprising same
KR101499750B1 (ko) 2006-02-27 2015-03-06 더 보드 오브 리전츠 오브 더 유니버시티 오브 텍사스 시스템 가감염성 플라비바이러스 및 이들의 용도
DK2099485T3 (en) 2006-11-03 2018-05-22 Alphavax Inc Alphavirus and alphavirus replica particle formulations and associated methods
BRPI0808084A2 (pt) 2007-02-27 2014-07-22 Int Inst Cancer Immunology Inc Método para ativação de células t auxiliadoras e composição para uso no método.
US8748591B2 (en) 2007-04-17 2014-06-10 The Board Of Regents Of The University Of Texas System Chimeric sindbis-western equine encephalitis virus and uses thereof
WO2008136438A1 (ja) * 2007-04-27 2008-11-13 Kyushu University, National University Corporation 遺伝子治療用ウイルスベクター
AU2008338803B2 (en) * 2007-11-26 2015-02-05 Glaxosmithkline Biologicals S.A. Methods of generating alphavirus particles
CA2940962C (en) 2007-12-05 2018-07-24 International Institute Of Cancer Immunology, Inc. Cancer vaccine composition
KR101668849B1 (ko) 2008-01-24 2016-10-24 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 모기에서 복제 불가능한 약독화된 재조합 알파바이러스 및 그의 용도
EP2130912A1 (en) 2008-06-04 2009-12-09 Institut für Viruskrankeiten und Immunprophylaxe Pestivirus replicons providing an RNA-based viral vector system
WO2010008054A1 (ja) 2008-07-16 2010-01-21 ディナベック株式会社 染色体非組み込み型ウイルスベクターを用いてリプログラムされた細胞を製造する方法
CA2766907A1 (en) 2009-07-06 2011-01-13 Novartis Ag Self replicating rna molecules and uses thereof
US9770463B2 (en) 2010-07-06 2017-09-26 Glaxosmithkline Biologicals Sa Delivery of RNA to different cell types
WO2012051211A2 (en) 2010-10-11 2012-04-19 Novartis Ag Antigen delivery platforms
WO2012106403A2 (en) 2011-02-01 2012-08-09 Uab Research Foundation Methods and compositions for pseudoinfectious alphaviruses
MX350258B (es) * 2011-07-06 2017-08-31 Novartis Ag Emulsiones cationicas de aceite en agua.
AU2013207669C1 (en) 2012-01-13 2018-05-31 Memorial Sloan Kettering Cancer Center Immunogenic WT-1 peptides and methods of use thereof
PL2852671T3 (pl) 2012-05-21 2019-06-28 The Regents Of The University Of California Wytwarzanie ludzkich komórek ips z użyciem syntetycznego samoreplikującego się rna
US8961995B2 (en) 2012-09-20 2015-02-24 Uab Research Foundation Methods and compositions for alphavirus replicons
US9447422B2 (en) 2012-12-06 2016-09-20 Synthetic Genomics, Inc. Autonomous replication sequences and episomal DNA molecules
RS61173B1 (sr) 2013-01-15 2021-01-29 Memorial Sloan Kettering Cancer Center Imunogeni wt-1 peptidi i postupci za njihovu upotrebu
US10815273B2 (en) 2013-01-15 2020-10-27 Memorial Sloan Kettering Cancer Center Immunogenic WT-1 peptides and methods of use thereof
KR102503695B1 (ko) 2013-02-28 2023-02-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 줄기세포를 동원하기 위한 방법 및 조성물
EP3782628B1 (en) 2013-03-15 2024-08-07 Elixirgen Therapeutics, Inc. Methods of using zscan4 for rejuvenating human cells
WO2014200910A2 (en) 2013-06-10 2014-12-18 Iogenetics, Llc Bioinformatic processes for determination of peptide binding
US10975358B2 (en) * 2013-09-24 2021-04-13 Id Pharma Co., Ltd. Method for improving efficiency of inducing pluripotent stem cell
EA039060B1 (ru) * 2013-12-20 2021-11-29 Бёрингер Ингельхайм Ветмедика Гмбх Вариант вируса ppcc, способ его получения с помощью молекулы нуклеиновой кислоты, конструкции днк или клетки и его применение для лечения и профилактики ррсс
US10266834B2 (en) 2014-02-13 2019-04-23 Synthetic Genomics, Inc. Recombinant RNA particles and methods of producing proteins
US10174317B2 (en) 2014-02-13 2019-01-08 Synthetic Genomics, Inc. Recombinant RNA particles and methods of use
US20180195085A1 (en) 2015-02-02 2018-07-12 Id Pharma Co., Ltd. Improved negative-strand rna viral vector
EP3061826A1 (en) 2015-02-27 2016-08-31 Novartis AG Flavivirus replicons
IL294183B2 (en) * 2015-05-20 2023-10-01 Dana Farber Cancer Inst Inc shared neoantigens
US10695385B2 (en) 2015-06-25 2020-06-30 National University Corporation Kobe University Oral cancer vaccine
WO2017015457A1 (en) 2015-07-21 2017-01-26 Modernatx, Inc. Ebola vaccine
EP3334755B1 (en) 2015-08-10 2020-04-08 Cellivery Therapeutics, Inc. Improved cell-permeable reprogramming factor (icp-rf) recombinant protein and use thereof
US10166281B2 (en) 2015-09-04 2019-01-01 Vlp Therapeutics, Llc Method and composition for modulating immune response
US12252700B2 (en) 2015-11-13 2025-03-18 Id Pharma Co., Ltd. Paramyxovirus vector
US10801041B2 (en) 2015-11-18 2020-10-13 Orbis Health Solutions, Llc T7 alpha viral vector system
SMT202200370T1 (it) 2015-11-20 2022-11-18 Memorial Sloan Kettering Cancer Center Composizione per il trattamento del cancro
GB201603374D0 (en) 2016-02-26 2016-04-13 Ucl Business Plc Packaging cell
WO2017162266A1 (en) 2016-03-21 2017-09-28 Biontech Rna Pharmaceuticals Gmbh Rna replicon for versatile and efficient gene expression
WO2017162265A1 (en) 2016-03-21 2017-09-28 Biontech Rna Pharmaceuticals Gmbh Trans-replicating rna
JP7250520B2 (ja) 2016-04-13 2023-04-03 ヤンセン ファーマシューティカルズ，インコーポレーテッド 組換えアルテリウイルスレプリコン系およびその使用
BE1024796B9 (fr) 2016-06-02 2019-01-08 Glaxosmithkline Biologicals Sa Constructions antigeniques du virus zika
US20190216956A1 (en) * 2016-07-27 2019-07-18 The Trustees Of Columbia University In The City Of New York Compositions for radiotherapy and uses thereof
KR102718353B1 (ko) 2016-10-17 2024-10-15 얀센 파마슈티칼즈, 인코포레이티드 재조합 바이러스 레플리콘 시스템 및 그의 용도
US10487105B2 (en) 2016-10-19 2019-11-26 Arcturus Therapeutics, Inc. Trinucleotide MRNA cap analogs
US10960070B2 (en) * 2016-10-25 2021-03-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Prefusion coronavirus spike proteins and their use
CA3045650A1 (en) 2016-12-05 2018-06-14 Janssen Pharmaceuticals, Inc. Compositions and methods for enhancing gene expression
WO2018115527A2 (en) 2016-12-23 2018-06-28 Curevac Ag Mers coronavirus vaccine
WO2018161092A1 (en) 2017-03-03 2018-09-07 New York University Induction and enhancement of antitumor immunity involving virus vectors expressing multiple epitopes of tumor associated antigens and immune checkpoint inhibitors or proteins
EP3595676A4 (en) * 2017-03-17 2021-05-05 Modernatx, Inc. RNA VACCINES AGAINST ZOONOSES
ES3039647T3 (en) 2017-05-31 2025-10-23 Arcturus Therapeutics Inc Synthesis and structure of high potency rna therapeutics
SG11202005792RA (en) 2017-12-20 2020-07-29 Vlp Therapeutics Llc Alphavirus replicon particle
JP7494117B2 (ja) 2018-01-19 2024-06-03 ヤンセン ファーマシューティカルズ，インコーポレーテッド 組換えレプリコン系を使用する免疫応答の誘導および増強
CA3101454A1 (en) 2018-05-30 2019-12-05 Translate Bio, Inc. Messenger rna vaccines and uses thereof
WO2021119545A1 (en) 2019-12-11 2021-06-17 Gritstone Bio, Inc. Durable vaccination
MX2022007905A (es) 2019-12-31 2022-08-25 Elixirgen Therapeutics Inc Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos.
KR20220128367A (ko) 2019-12-31 2022-09-20 엘릭서젠 쎄라퓨틱스, 인크. Zscan4 핵산 및 단백질의 세포 및 조직으로의 온도-기반 일시적 전달
EP4142786A4 (en) 2020-04-27 2024-06-19 Ohio State Innovation Foundation LIVE ATTENUATED MEASLES VIRUS VACCINE AGAINST SARS-COV-2
WO2021255270A1 (en) 2020-06-19 2021-12-23 Ziphius Vaccines Self-amplifying sars-cov-2 rna vaccine

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