MX2022007905A - Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos. - Google Patents

Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos.

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Publication number
MX2022007905A
MX2022007905A MX2022007905A MX2022007905A MX2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A MX 2022007905 A MX2022007905 A MX 2022007905A
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MX
Mexico
Prior art keywords
temperature
cells
subject
sensitive
methods
Prior art date
Application number
MX2022007905A
Other languages
English (en)
Inventor
Minoru S H Ko
Original Assignee
Elixirgen Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elixirgen Therapeutics Inc filed Critical Elixirgen Therapeutics Inc
Publication of MX2022007905A publication Critical patent/MX2022007905A/es

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  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pulmonology (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)

Abstract

La presente descripción se refiere a métodos para activar transitoriamente agentes sensibles a la temperatura en una o más células, por ejemplo, al poner en contacto una o más células con un agente sensible a la temperatura y al incubar transitoriamente las células a una temperatura permisiva para inducir en las células una actividad del agente sensible a la temperatura. Adicionalmente, la presente descripción se refiere a métodos para poner en contacto una o más células en un sujeto con un agente sensible a la temperatura y luego reducir la temperatura corporal central del sujeto a una temperatura permisiva para inducir una actividad del agente sensible a la temperatura en las células. La descripción también se refiere a métodos para poner en contacto una o más células en un sujeto con un agente sensible a la temperatura, manteniendo la temperatura corporal superficial del sujeto a una temperatura permisiva para inducir en las células una actividad del agente sensible a la temperatura. Se describen adicionalmente métodos para tratar a un sujeto con un agente terapéutico sensible a la temperatura.
MX2022007905A 2019-12-31 2020-12-30 Administracion transitoria basada en temperatura de acidos nucleicos y proteinas a celulas y tejidos. MX2022007905A (es)

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Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220129567A (ko) 2019-12-31 2022-09-23 엘릭서젠 쎄라퓨틱스, 인크. 핵산 및 단백질의 세포 및 조직으로의 온도-기반 일시적 전달
CN115867658A (zh) * 2020-04-17 2023-03-28 Vlp治疗公司 冠状病毒疫苗
GB202307565D0 (en) 2020-04-22 2023-07-05 BioNTech SE Coronavirus vaccine
IL309038A (en) * 2021-06-17 2024-02-01 Elixirgen Therapeutics Inc Temperature-controlled and self-replicating RNA constructs for viral diseases
EP4395817A1 (en) * 2021-09-02 2024-07-10 Elixirgen Therapeutics, Inc. Temperature-controllable, rna immunotherapeutic for cancer
WO2023150285A1 (en) 2022-02-04 2023-08-10 Elixirgen Therapeutics, Inc. Cell therapy protocol systems and methods
US11878055B1 (en) 2022-06-26 2024-01-23 BioNTech SE Coronavirus vaccine
WO2024112821A1 (en) * 2022-11-22 2024-05-30 Elixirgen Therapeutics, Inc. Antigens for cancer immunotherapy

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5217879A (en) 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
ATE340254T1 (de) 1995-04-06 2006-10-15 Miltenyi Biotec Inc Verfahren zur freisetzung von magnetischen teilchen, die an zelloberflächen gebunden sind
US6458560B1 (en) 1996-04-05 2002-10-01 Chiron Corporation Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
US6451592B1 (en) 1996-04-05 2002-09-17 Chiron Corporation Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
CA2325564A1 (en) 1998-03-27 1999-10-07 Cytos Biotechnology Ag Inducible alphaviral gene expression system
JP4422903B2 (ja) 1998-07-31 2010-03-03 株式会社癌免疫研究所 癌抑制遺伝子ｗｔ１の産物に基づく癌抗原
US8647864B2 (en) 1999-04-14 2014-02-11 Novartis Ag Compositions and methods for generating an immune response utilizing alphavirus-based vector systems
CA2373300C (en) 1999-04-14 2012-02-21 Chiron Corporation Compositions and methods for generating an immune response utilizing alphavirus-based vector systems
DE60035589T2 (de) 1999-05-18 2008-04-17 Dnavec Research Inc., Tsukuba Viraler paramyxoviridae vektor mit einem defekten hüllprotein-gen
US20030232324A1 (en) 2001-05-31 2003-12-18 Chiron Corporation Chimeric alphavirus replicon particles
EP1399183B1 (en) 2001-05-31 2010-06-30 Novartis Vaccines and Diagnostics, Inc. Chimeric alphavirus replicon particles
DE60233061D1 (de) 2001-09-06 2009-09-03 Alphavax Inc Alphavirus replikon-vektorsysteme
US7244434B2 (en) 2002-08-13 2007-07-17 Intervet International B.V. Replicons of pestviruses that do not express C and or E1 protein and infectious viral particles containing same, that can be used in vaccines
WO2004027037A2 (en) * 2002-09-18 2004-04-01 The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services RECOVERY OF RECOMBINANT HUMAN PARAINFLUENZA VIRUS TYPE 2 (HPIV2) FROM cDNA AND USE OF RECOMBINANT HPIV2 IN IMMUNOGENIC COMPOSITIONS AND AS VECTORS TO ELICIT IMMUNE RESPONSES AGAINST PIV AND OTHER HUMAN PATHOGENS
WO2004055161A2 (en) 2002-12-12 2004-07-01 The Board Of Regents Of The University Of Texas System Large scale production of packaged alphavirus replicons
US7078218B2 (en) 2002-12-13 2006-07-18 Alphavax, Inc. Alphavirus particles and methods for preparation
CA2518546C (en) 2003-03-20 2012-11-13 Alphavax, Inc. Improved alphavirus replicons and helper constructs
US20050282279A1 (en) * 2003-05-29 2005-12-22 Hwu Paul L Expression vector encoding coronavirus-like particle
BRPI0411099A (pt) 2003-06-05 2006-07-18 Wyeth Corp composições imunogênicas que compreendem os vetores de replicon do vìrus da encefalite eqüina venezuelana e antìgenos de proteìnas de paramixovìrus
ATE420965T1 (de) 2004-05-18 2009-01-15 Alphavax Inc Von tc-83 abgeleitete alphavirus-vektoren, partikel und verfahren
US7332322B2 (en) 2004-09-14 2008-02-19 Ilya Frolov Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
US7998733B2 (en) * 2004-10-05 2011-08-16 Merial Limited Chimeric vectors
JP4478788B2 (ja) 2005-05-27 2010-06-09 独立行政法人産業技術総合研究所 センダイウイルス温度感受性株由来のウイルスベクター
ES2735531T3 (es) 2005-08-23 2019-12-19 Univ Pennsylvania ARN que contiene nucleósidos modificados y métodos de uso del mismo
EP1951281B1 (en) 2005-10-17 2015-04-15 Sloan Kettering Institute For Cancer Research Wt1 hla class ii-binding peptides and compositions and methods comprising same
JP5538729B2 (ja) 2006-02-27 2014-07-02 ザ ボード オブ リージェンツ オブ ザ ユニバーシティー オブ テキサス システム 偽感染性フラビウイルスおよびそれらの使用
DK2099485T3 (en) 2006-11-03 2018-05-22 Alphavax Inc Alphavirus and alphavirus replica particle formulations and associated methods
MX352947B (es) 2007-02-27 2017-12-14 Int Inst Cancer Immunology Inc Metodo para activacion de celula t auxiliar y composicion para usarse en el metodo.
US8748591B2 (en) 2007-04-17 2014-06-10 The Board Of Regents Of The University Of Texas System Chimeric sindbis-western equine encephalitis virus and uses thereof
EP2143794A1 (en) * 2007-04-27 2010-01-13 Kyushu University, National University Corporation Viral vector for gene therapy
US9234181B2 (en) * 2007-11-26 2016-01-12 Novartis Ag RNA expression cassette and cells for making alphavirus particles
ES2679127T3 (es) 2007-12-05 2018-08-22 International Institute Of Cancer Immunology, Inc. Composición vacunal contra el cáncer
CA2910235C (en) 2008-01-24 2021-07-06 Scott C. Weaver Attenuated recombinant alphaviruses incapable of replicating in mosquitoes and uses thereof
EP2130912A1 (en) 2008-06-04 2009-12-09 Institut für Viruskrankeiten und Immunprophylaxe Pestivirus replicons providing an RNA-based viral vector system
EP3075850B1 (en) 2008-07-16 2019-02-06 ID Pharma Co., Ltd. Method for production of reprogrammed cell using chromosomally unintegrated virus vector
CA2766907A1 (en) 2009-07-06 2011-01-13 Novartis Ag Self replicating rna molecules and uses thereof
US9770463B2 (en) 2010-07-06 2017-09-26 Glaxosmithkline Biologicals Sa Delivery of RNA to different cell types
TR201903651T4 (tr) 2010-10-11 2019-04-22 Glaxosmithkline Biologicals Sa Antijen uygulama platformları.
WO2012106403A2 (en) 2011-02-01 2012-08-09 Uab Research Foundation Methods and compositions for pseudoinfectious alphaviruses
ES2702318T3 (es) * 2011-07-06 2019-02-28 Glaxosmithkline Biologicals Sa Emulsiones catiónicas de aceite en agua
US20150104413A1 (en) 2012-01-13 2015-04-16 Memorial Sloan Kettering Cancer Center Immunogenic wt-1 peptides and methods of use thereof
CN104508131B (zh) 2012-05-21 2018-11-23 加利福尼亚大学董事会 通过合成的自我复制的RNA形成人iPS细胞
US8961995B2 (en) 2012-09-20 2015-02-24 Uab Research Foundation Methods and compositions for alphavirus replicons
WO2014089513A1 (en) 2012-12-06 2014-06-12 Synthetic Genomics, Inc. Autonomous replication sequences and episomal dna molecules
US10815273B2 (en) 2013-01-15 2020-10-27 Memorial Sloan Kettering Cancer Center Immunogenic WT-1 peptides and methods of use thereof
HUE052541T2 (hu) 2013-01-15 2021-05-28 Memorial Sloan Kettering Cancer Center Immunogén WT-1 peptidek és eljárások azok alkalmazására
EP2961828A4 (en) 2013-02-28 2016-08-03 Harvard College COMPOSITIONS AND METHOD FOR MOBILIZING STEM CELLS
SG11201507334VA (en) 2013-03-15 2015-10-29 Elixirgen Llc Methods of using zscan4 for rejuvenating human cells
WO2014200910A2 (en) 2013-06-10 2014-12-18 Iogenetics, Llc Bioinformatic processes for determination of peptide binding
US10975358B2 (en) * 2013-09-24 2021-04-13 Id Pharma Co., Ltd. Method for improving efficiency of inducing pluripotent stem cell
EA039060B1 (ru) * 2013-12-20 2021-11-29 Бёрингер Ингельхайм Ветмедика Гмбх Вариант вируса ppcc, способ его получения с помощью молекулы нуклеиновой кислоты, конструкции днк или клетки и его применение для лечения и профилактики ррсс
WO2015123592A2 (en) 2014-02-13 2015-08-20 Synthetic Genomics, Inc. Recombinant rna particles and methods of use
US10266834B2 (en) 2014-02-13 2019-04-23 Synthetic Genomics, Inc. Recombinant RNA particles and methods of producing proteins
WO2016125364A1 (ja) 2015-02-02 2016-08-11 株式会社Ｉｄファーマ 改良されたマイナス鎖ｒｎａウイルスベクター
EP3061826A1 (en) 2015-02-27 2016-08-31 Novartis AG Flavivirus replicons
CN116196401A (zh) * 2015-05-20 2023-06-02 博德研究所 共有的新抗原
JP6770269B2 (ja) 2015-06-25 2020-10-14 国立大学法人神戸大学 経口腫瘍ワクチン
WO2017026776A1 (en) 2015-08-10 2017-02-16 Cellivery Therapeutics, Inc. Improved cell-permeable reprogramming factor (icp-rf) recombinant protein and use thereof
US10166281B2 (en) 2015-09-04 2019-01-01 Vlp Therapeutics, Llc Method and composition for modulating immune response
WO2017082174A1 (ja) 2015-11-13 2017-05-18 株式会社Ｉｄファーマ 改良されたパラミクソウイルスベクター
SG11201804197RA (en) 2015-11-18 2018-06-28 Orbis Health Solutions Llc T7 alpha viral vector system
WO2017087857A1 (en) 2015-11-20 2017-05-26 Memorial Sloan Kettering Cancer Center Methods and compositions for treating cancer
GB201603374D0 (en) 2016-02-26 2016-04-13 Ucl Business Plc Packaging cell
WO2017162266A1 (en) 2016-03-21 2017-09-28 Biontech Rna Pharmaceuticals Gmbh Rna replicon for versatile and efficient gene expression
WO2017162265A1 (en) 2016-03-21 2017-09-28 Biontech Rna Pharmaceuticals Gmbh Trans-replicating rna
CA3020426A1 (en) 2016-04-13 2017-10-19 Synthetic Genomics, Inc. Recombinant arterivirus replicon systems and uses thereof
BE1024796B9 (fr) 2016-06-02 2019-01-08 Glaxosmithkline Biologicals Sa Constructions antigeniques du virus zika
US20190216956A1 (en) * 2016-07-27 2019-07-18 The Trustees Of Columbia University In The City Of New York Compositions for radiotherapy and uses thereof
EP3526332B1 (en) 2016-10-17 2024-06-26 Janssen Pharmaceuticals, Inc. Recombinant virus replicon systems and uses thereof
EP3529255A1 (en) 2016-10-19 2019-08-28 Arcturus Therapeutics, Inc. Trinucleotide mrna cap analogs
WO2018081318A1 (en) * 2016-10-25 2018-05-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Prefusion coronavirus spike proteins and their use
EP3548625B1 (en) 2016-12-05 2024-06-26 Janssen Pharmaceuticals, Inc. Compositions and methods for enhancing gene expression
US20200030432A1 (en) * 2017-03-17 2020-01-30 Modernatx, Inc. Zoonotic disease rna vaccines
EP3630985A4 (en) 2017-05-31 2021-06-09 Arcturus Therapeutics, Inc. SYNTHESIS AND STRUCTURE OF HIGH POWER RNA-BASED THERAPEUTIC AGENTS
BR112020012190A8 (pt) 2017-12-20 2023-04-11 Vlp Therapeutics Llc Partícula de replicon de alfavírus
EP3740245A4 (en) 2018-01-19 2022-01-05 Janssen Pharmaceuticals, Inc. INDUCTION AND ENHANCEMENT OF IMMUNE RESPONSES USING RECOMBINATION REPLICON SYSTEMS
AU2019277361A1 (en) 2018-05-30 2020-12-17 Translate Bio, Inc. Messenger RNA vaccines and uses thereof
WO2021119545A1 (en) 2019-12-11 2021-06-17 Gritstone Bio, Inc. Durable vaccination
KR20220128367A (ko) 2019-12-31 2022-09-20 엘릭서젠 쎄라퓨틱스, 인크. Zscan4 핵산 및 단백질의 세포 및 조직으로의 온도-기반 일시적 전달
KR20220129567A (ko) 2019-12-31 2022-09-23 엘릭서젠 쎄라퓨틱스, 인크. 핵산 및 단백질의 세포 및 조직으로의 온도-기반 일시적 전달

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