MX2021000605A - Producción de glucósidos de esteviol a través de biotransformación de células enteras. - Google Patents

Producción de glucósidos de esteviol a través de biotransformación de células enteras.

Info

Publication number
MX2021000605A
MX2021000605A MX2021000605A MX2021000605A MX2021000605A MX 2021000605 A MX2021000605 A MX 2021000605A MX 2021000605 A MX2021000605 A MX 2021000605A MX 2021000605 A MX2021000605 A MX 2021000605A MX 2021000605 A MX2021000605 A MX 2021000605A
Authority
MX
Mexico
Prior art keywords
intermediates
production
whole cell
steviol glycosides
glycosylation
Prior art date
Application number
MX2021000605A
Other languages
English (en)
Spanish (es)
Inventor
Ajikumar Parayil Kumaran
Aaron Love
Christine Nicole S Santos
Jason Eric Donald
Mary Elizabeth Fowler
Ryan N Philippe
Christopher Scott Frei
Original Assignee
Manus Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manus Bio Inc filed Critical Manus Bio Inc
Publication of MX2021000605A publication Critical patent/MX2021000605A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01022—UDP-glucose 6-dehydrogenase (1.1.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02009—Uracil phosphoribosyltransferase (2.4.2.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04022—UMP kinase (2.7.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07024—Glucose-1-phosphate thymidylyltransferase (2.7.7.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07027—Glucose-1-phosphate adenylyltransferase (2.7.7.27), i.e. ADP-glucose pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/0301—Glucose-1-phosphatase (3.1.3.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02006—Beta-phosphoglucomutase (5.4.2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07012—UDP-glucose--hexose-1-phosphate uridylyltransferase (2.7.7.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01045—UDP-sugar diphosphatase (3.6.1.45)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03002—UDP-glucose 4-epimerase (5.1.3.2), i.e. UDP-galactose 4-epimerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
MX2021000605A 2018-07-16 2019-07-16 Producción de glucósidos de esteviol a través de biotransformación de células enteras. MX2021000605A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862698617P 2018-07-16 2018-07-16
PCT/US2019/041957 WO2020018506A2 (en) 2018-07-16 2019-07-16 Production of steviol glycosides through whole cell biotransformation

Publications (1)

Publication Number Publication Date
MX2021000605A true MX2021000605A (es) 2021-06-23

Family

ID=69164862

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021000605A MX2021000605A (es) 2018-07-16 2019-07-16 Producción de glucósidos de esteviol a través de biotransformación de células enteras.

Country Status (14)

Country Link
US (2) US11230724B2 (https=)
EP (2) EP3824093B1 (https=)
JP (1) JP7305087B2 (https=)
KR (1) KR102532079B1 (https=)
CN (1) CN112867799B (https=)
AU (1) AU2019304965B2 (https=)
BR (1) BR112021000876A2 (https=)
CA (1) CA3106633A1 (https=)
ES (1) ES3013059T3 (https=)
IL (1) IL280151A (https=)
MX (1) MX2021000605A (https=)
MY (1) MY204735A (https=)
SG (1) SG11202100450TA (https=)
WO (1) WO2020018506A2 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111868252B (zh) * 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
CN109402152B (zh) * 2018-11-09 2021-08-13 沈阳农业大学 一种表达制备udp-葡萄糖-4-差向异构酶的方法
CN109295087B (zh) * 2018-11-09 2021-08-24 沈阳农业大学 一种表达制备udp-葡萄糖-己糖-1-磷酸尿苷酰转移酶的方法
WO2020264179A1 (en) 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
CN110699373B (zh) * 2019-10-16 2023-05-26 中国药科大学 尿苷二磷酸葡萄糖高产菌株及其应用
CN111518825B (zh) * 2020-04-30 2022-10-11 浙江工业大学 一种多基因组合表达制备蛹虫草多糖的方法
US20230174993A1 (en) * 2020-05-13 2023-06-08 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
WO2022002918A1 (en) * 2020-07-03 2022-01-06 C-Lecta Gmbh One-pot cell-free glycosylation process
CA3200689A1 (en) * 2020-11-24 2022-06-02 Manus Bio Inc. Glycoside product biosynthesis and recovery
US20250320470A1 (en) * 2020-12-23 2025-10-16 Samyang Corporation Glycosyltransferase and steviol glucoside preparation method using same
JP7720917B2 (ja) * 2021-01-27 2025-08-08 コリア アドバンスト インスティテュート オブ サイエンス アンド テクノロジー Ｃ－グリコシルトランスフェラーゼ変異体およびその用途
WO2023000243A1 (zh) * 2021-07-22 2023-01-26 深圳先进技术研究院 重组酵母生物转化生产葡萄糖的方法
WO2023121426A1 (ko) * 2021-12-24 2023-06-29 주식회사 삼양사 레바우디오사이드의 생산
CN114540396A (zh) * 2022-02-24 2022-05-27 天津大学 希瓦氏菌株中葡萄糖代谢通路的构建方法
CN114561310B (zh) * 2022-03-17 2022-12-02 江南大学 一种生产甜茶苷的酿酒酵母及其应用
WO2024121721A1 (en) * 2022-12-05 2024-06-13 Tate & Lyle Solutions Usa Llc Methods for obtaining steviol glycosides
WO2024146325A1 (zh) * 2023-01-06 2024-07-11 中国人民解放军军事科学院军事医学研究院 用于生产抗原多糖-载体蛋白生物偶联产物的工程菌及其应用
CN115975902B (zh) * 2023-01-31 2025-07-01 深圳中科欣扬生物科技有限公司 一种全细胞催化合成阿魏酸葡萄糖苷的基因工程菌及其应用
CN118240855A (zh) * 2024-03-22 2024-06-25 百开盛(上海)生物科技有限公司 Udpg生产菌株及其构建方法和应用
CN118374427B (zh) * 2024-05-07 2025-01-24 山东三元生物科技股份有限公司 一株催化甜菊糖粗糖生成莱鲍迪甙d的重组菌及其应用
WO2025238040A1 (en) * 2024-05-16 2025-11-20 Evonik Operations Gmbh A novel glucolipid

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586214B1 (en) * 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
CN105671108A (zh) * 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
CN108396044A (zh) * 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
BR112014028819A2 (pt) * 2012-05-22 2017-07-25 Purecircle Sdn Bhd glicosídeos de esteviol de pureza elevada
CN103397064B (zh) * 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
EP3215629B1 (en) 2014-11-05 2024-08-07 Manus Bio Inc. Microbial production of steviol glycosides
US20180057850A1 (en) * 2015-03-23 2018-03-01 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
KR101669041B1 (ko) * 2015-03-31 2016-10-25 아주대학교산학협력단 스테비오사이드 생산능을 가지는 재조합 미생물 및 이를 이용한 스테비오사이드의 생산 방법
US20190169664A1 (en) 2016-05-06 2019-06-06 University Of Georgia Research Foundation, Inc. Microbial platform for production of glycosylated compounds
CA3175070A1 (en) * 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
WO2020264179A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme

Also Published As

Publication number Publication date
US20220162658A1 (en) 2022-05-26
EP4509609A3 (en) 2025-07-23
CA3106633A1 (en) 2020-01-23
JP2021530228A (ja) 2021-11-11
IL280151A (en) 2021-03-01
CN112867799B (zh) 2024-09-24
US20200087692A1 (en) 2020-03-19
AU2019304965B2 (en) 2025-04-24
EP3824093A2 (en) 2021-05-26
JP7305087B2 (ja) 2023-07-10
KR20210066790A (ko) 2021-06-07
EP3824093B1 (en) 2024-11-20
SG11202100450TA (en) 2021-02-25
EP3824093A4 (en) 2022-06-01
KR102532079B1 (ko) 2023-05-12
US11230724B2 (en) 2022-01-25
EP4509609A2 (en) 2025-02-19
BR112021000876A2 (pt) 2021-04-13
US12012626B2 (en) 2024-06-18
AU2019304965A1 (en) 2021-02-11
WO2020018506A3 (en) 2020-03-12
WO2020018506A2 (en) 2020-01-23
CN112867799A (zh) 2021-05-28
MY204735A (en) 2024-09-11
ES3013059T3 (en) 2025-04-10

Similar Documents

Publication Publication Date Title
MX2021000605A (es) Producción de glucósidos de esteviol a través de biotransformación de células enteras.
MX2022003824A (es) Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MY194280A (en) Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
BR112018008864A2 (pt) meios e métodos de fermentação para a produção de polissacarídeos em uma cultura de células bacterianas
WO2013022881A8 (en) Compositions and methods for the biosynthesis of vanillin or vanillin beta-d-glucoside
MX373909B (es) Produccion recombinante de esteviol glucosidos.
WO2008142036A8 (en) Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-ko
AU2012294452A8 (en) Recombinant production of steviol glycosides
MX2016014506A (es) Edulcorante no calorico.
WO2016071505A9 (en) Microbial production of the flavonoids garbanzol, resokaempferol and fisetin
WO2011134919A3 (en) Cho cells cultivation process 0
BR112021024657A2 (pt) Célula de bacillus não esporulada, uso da referida célula, oligossacarídeo fucosilado, bem como o método para a produção e uso do mesmo e composição nutricional
MX2009011109A (es) Glicosilación tipo mamífera en plantas para la expresión de glicosil-transferasas no mamíferas.
MX2021004099A (es) Microbioreactor de flujo continuo.
MX2016010236A (es) Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante.
PH12018502655A1 (en) Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate
AU2018274709A1 (en) Methods for increasing grain productivity
MX2021005948A (es) Biosintesis de compuestos en levadura.
WO2019217493A3 (en) Cell populations and gene expression associated with in vitro beta cell differentiation
WO2018114839A3 (en) Production of manool
NZ749715A (en) Leuconostoc mesenteroides cjlm119 strain producing reduced amount of gas, and kimchi production method using same
MY188848A (en) Yeast propagation simultanesous with saccharification
MX2020001537A (es) Kaureno oxidasa de pisum sativum para la produccion de alta eficiencia de rebaudiosidos.
MX2019004630A (es) Metodo para preparar rebaudiosido j usando el metodo enzimatico.
AU2016427120A8 (en) Method for preparing rebaudioside C using enzymatic method