BR112021000876A2 - Produção de glicosídeos de esteviol através da biotransformação com células inteiras - Google Patents

Produção de glicosídeos de esteviol através da biotransformação com células inteiras Download PDF

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BR112021000876A2
BR112021000876A2 BR112021000876-4A BR112021000876A BR112021000876A2 BR 112021000876 A2 BR112021000876 A2 BR 112021000876A2 BR 112021000876 A BR112021000876 A BR 112021000876A BR 112021000876 A2 BR112021000876 A2 BR 112021000876A2
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fact
microbial cell
coli
glucose
glycosylation
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BR112021000876-4A
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English (en)
Portuguese (pt)
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Ajikumar Parayil KUMARAN
Christine Nicole S. Santos
Jason Eric DONALD
Mary Elizabeth FOWLER
Ryan N. PHILIPPE
Christopher Scott FREI
Aaron Love
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Manus Bio, Inc.
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BR112021000876-4A 2018-07-16 2019-07-16 Produção de glicosídeos de esteviol através da biotransformação com células inteiras BR112021000876A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862698617P 2018-07-16 2018-07-16
US62/698,617 2018-07-16
PCT/US2019/041957 WO2020018506A2 (en) 2018-07-16 2019-07-16 Production of steviol glycosides through whole cell biotransformation

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BR112021000876A2 true BR112021000876A2 (pt) 2021-04-13

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US (2) US11230724B2 (https=)
EP (2) EP3824093B1 (https=)
JP (1) JP7305087B2 (https=)
KR (1) KR102532079B1 (https=)
CN (1) CN112867799B (https=)
AU (1) AU2019304965B2 (https=)
BR (1) BR112021000876A2 (https=)
CA (1) CA3106633A1 (https=)
ES (1) ES3013059T3 (https=)
IL (1) IL280151A (https=)
MX (1) MX2021000605A (https=)
MY (1) MY204735A (https=)
SG (1) SG11202100450TA (https=)
WO (1) WO2020018506A2 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111868252B (zh) * 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
CN109402152B (zh) * 2018-11-09 2021-08-13 沈阳农业大学 一种表达制备udp-葡萄糖-4-差向异构酶的方法
CN109295087B (zh) * 2018-11-09 2021-08-24 沈阳农业大学 一种表达制备udp-葡萄糖-己糖-1-磷酸尿苷酰转移酶的方法
WO2020264179A1 (en) 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
CN110699373B (zh) * 2019-10-16 2023-05-26 中国药科大学 尿苷二磷酸葡萄糖高产菌株及其应用
CN111518825B (zh) * 2020-04-30 2022-10-11 浙江工业大学 一种多基因组合表达制备蛹虫草多糖的方法
US20230174993A1 (en) * 2020-05-13 2023-06-08 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
WO2022002918A1 (en) * 2020-07-03 2022-01-06 C-Lecta Gmbh One-pot cell-free glycosylation process
CA3200689A1 (en) * 2020-11-24 2022-06-02 Manus Bio Inc. Glycoside product biosynthesis and recovery
US20250320470A1 (en) * 2020-12-23 2025-10-16 Samyang Corporation Glycosyltransferase and steviol glucoside preparation method using same
JP7720917B2 (ja) * 2021-01-27 2025-08-08 コリア アドバンスト インスティテュート オブ サイエンス アンド テクノロジー C-グリコシルトランスフェラーゼ変異体およびその用途
WO2023000243A1 (zh) * 2021-07-22 2023-01-26 深圳先进技术研究院 重组酵母生物转化生产葡萄糖的方法
WO2023121426A1 (ko) * 2021-12-24 2023-06-29 주식회사 삼양사 레바우디오사이드의 생산
CN114540396A (zh) * 2022-02-24 2022-05-27 天津大学 希瓦氏菌株中葡萄糖代谢通路的构建方法
CN114561310B (zh) * 2022-03-17 2022-12-02 江南大学 一种生产甜茶苷的酿酒酵母及其应用
WO2024121721A1 (en) * 2022-12-05 2024-06-13 Tate & Lyle Solutions Usa Llc Methods for obtaining steviol glycosides
WO2024146325A1 (zh) * 2023-01-06 2024-07-11 中国人民解放军军事科学院军事医学研究院 用于生产抗原多糖-载体蛋白生物偶联产物的工程菌及其应用
CN115975902B (zh) * 2023-01-31 2025-07-01 深圳中科欣扬生物科技有限公司 一种全细胞催化合成阿魏酸葡萄糖苷的基因工程菌及其应用
CN118240855A (zh) * 2024-03-22 2024-06-25 百开盛(上海)生物科技有限公司 Udpg生产菌株及其构建方法和应用
CN118374427B (zh) * 2024-05-07 2025-01-24 山东三元生物科技股份有限公司 一株催化甜菊糖粗糖生成莱鲍迪甙d的重组菌及其应用
WO2025238040A1 (en) * 2024-05-16 2025-11-20 Evonik Operations Gmbh A novel glucolipid

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586214B1 (en) * 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
CN105671108A (zh) * 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
CN108396044A (zh) * 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
BR112014028819A2 (pt) * 2012-05-22 2017-07-25 Purecircle Sdn Bhd glicosídeos de esteviol de pureza elevada
CN103397064B (zh) * 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
EP3215629B1 (en) 2014-11-05 2024-08-07 Manus Bio Inc. Microbial production of steviol glycosides
US20180057850A1 (en) * 2015-03-23 2018-03-01 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
KR101669041B1 (ko) * 2015-03-31 2016-10-25 아주대학교산학협력단 스테비오사이드 생산능을 가지는 재조합 미생물 및 이를 이용한 스테비오사이드의 생산 방법
US20190169664A1 (en) 2016-05-06 2019-06-06 University Of Georgia Research Foundation, Inc. Microbial platform for production of glycosylated compounds
CA3175070A1 (en) * 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
WO2020264179A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme

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