SG11202100450TA - Production of steviol glycosides through whole cell biotransformation - Google Patents

Production of steviol glycosides through whole cell biotransformation

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Publication number
SG11202100450TA
SG11202100450TA SG11202100450TA SG11202100450TA SG11202100450TA SG 11202100450T A SG11202100450T A SG 11202100450TA SG 11202100450T A SG11202100450T A SG 11202100450TA SG 11202100450T A SG11202100450T A SG 11202100450TA SG 11202100450T A SG11202100450T A SG 11202100450TA
Authority
SG
Singapore
Prior art keywords
production
whole cell
steviol glycosides
cell biotransformation
biotransformation
Prior art date
Application number
SG11202100450TA
Inventor
Ajikumar Kumaran
Christine Santos
Jason Donald
Mary Fowler
Ryan Philippe
Christopher Frei
Aaron Love
Original Assignee
Manus Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manus Bio Inc filed Critical Manus Bio Inc
Publication of SG11202100450TA publication Critical patent/SG11202100450TA/en

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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01022—UDP-glucose 6-dehydrogenase (1.1.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02009—Uracil phosphoribosyltransferase (2.4.2.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04022—UMP kinase (2.7.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07024—Glucose-1-phosphate thymidylyltransferase (2.7.7.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07027—Glucose-1-phosphate adenylyltransferase (2.7.7.27), i.e. ADP-glucose pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/0301—Glucose-1-phosphatase (3.1.3.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01045—UDP-sugar diphosphatase (3.6.1.45)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)
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    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02006—Beta-phosphoglucomutase (5.4.2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202100450TA 2018-07-16 2019-07-16 Production of steviol glycosides through whole cell biotransformation SG11202100450TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862698617P 2018-07-16 2018-07-16
PCT/US2019/041957 WO2020018506A2 (en) 2018-07-16 2019-07-16 Production of steviol glycosides through whole cell biotransformation

Publications (1)

Publication Number Publication Date
SG11202100450TA true SG11202100450TA (en) 2021-02-25

Family

ID=69164862

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202100450TA SG11202100450TA (en) 2018-07-16 2019-07-16 Production of steviol glycosides through whole cell biotransformation

Country Status (11)

Country Link
US (2) US11230724B2 (en)
EP (1) EP3824093A4 (en)
JP (1) JP7305087B2 (en)
KR (1) KR102532079B1 (en)
AU (1) AU2019304965A1 (en)
BR (1) BR112021000876A2 (en)
CA (1) CA3106633A1 (en)
IL (1) IL280151A (en)
MX (1) MX2021000605A (en)
SG (1) SG11202100450TA (en)
WO (1) WO2020018506A2 (en)

Families Citing this family (11)

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Publication number Priority date Publication date Assignee Title
WO2019178116A1 (en) * 2018-03-12 2019-09-19 Conagen Inc. Biosynthetic production of steviol glycosides rebaudioside j and rebaudioside n
JP7305087B2 (en) * 2018-07-16 2023-07-10 マナス バイオ インコーポレイテッド Production of steviol glycosides by whole-cell biotransformation
CN109295087B (en) * 2018-11-09 2021-08-24 沈阳农业大学 Method for expression preparation of UDP-glucose-hexose-1-phosphate uridyltransferase
CN109402152B (en) * 2018-11-09 2021-08-13 沈阳农业大学 Method for expression preparation of UDP-glucose-4-epimerase
JP2022530706A (en) * 2019-06-25 2022-06-30 マナス バイオ インコーポレイテッド Uridine diphosphate-dependent glycosyltransferase
CA3177183A1 (en) * 2020-05-13 2021-11-18 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
JP2024500432A (en) * 2020-12-23 2024-01-09 サムヤン コーポレイション Glycosyltransferase and method for producing steviol glycoside using the same
WO2023000243A1 (en) * 2021-07-22 2023-01-26 深圳先进技术研究院 Method for producing glucose by recombinant yeast biotransformation
KR20230098493A (en) * 2021-12-24 2023-07-04 주식회사 삼양사 Rebaudioside production
CN114540396A (en) * 2022-02-24 2022-05-27 天津大学 Construction method of glucose metabolic pathway in Shewanella strain
WO2024121721A1 (en) * 2022-12-05 2024-06-13 Tate & Lyle Solutions Usa Llc Methods for obtaining steviol glycosides

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US6586214B1 (en) * 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
BR122021005283B1 (en) * 2010-06-02 2022-02-22 Evolva, Inc RECOMBINANT HOST COMPRISING RECOMBINANT GENES FOR PRODUCTION OF STEVIOL OR STEVIOL GLYCOSIDE, METHOD FOR PRODUCING STEVIOL, STEVIOL GLYCOSIDE OR STEVIOL GLYCOSIDE COMPOSITION AND METHOD FOR SYNTHESISTING STEVIOL OR STEVIOL GLYCOSIDE
EP3009508B1 (en) * 2011-08-08 2020-11-25 Evolva SA Recombinant production of steviol glycosides
MX352678B (en) 2012-05-22 2017-12-04 Purecircle Sdn Bhd High-purity steviol glycosides.
CN103397064B (en) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 Method for preparing rebaudioside M through enzyme method
US10463062B2 (en) * 2014-11-05 2019-11-05 Manus Bio Inc. Microbial production of steviol glycosides
CN107429238A (en) * 2015-03-23 2017-12-01 帝斯曼知识产权资产管理有限公司 UDP glycosyl transferases from tomato
KR101669041B1 (en) 2015-03-31 2016-10-25 아주대학교산학협력단 Recombinant microoganisms for producing stevioside and method for stevioside using the same
US20190169664A1 (en) * 2016-05-06 2019-06-06 University Of Georgia Research Foundation, Inc. Microbial platform for production of glycosylated compounds
CA3050310A1 (en) * 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
JP7305087B2 (en) * 2018-07-16 2023-07-10 マナス バイオ インコーポレイテッド Production of steviol glycosides by whole-cell biotransformation
JP2022530706A (en) * 2019-06-25 2022-06-30 マナス バイオ インコーポレイテッド Uridine diphosphate-dependent glycosyltransferase

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EP3824093A4 (en) 2022-06-01
WO2020018506A2 (en) 2020-01-23
CN112867799A (en) 2021-05-28
AU2019304965A1 (en) 2021-02-11
CA3106633A1 (en) 2020-01-23
BR112021000876A2 (en) 2021-04-13
IL280151A (en) 2021-03-01
US11230724B2 (en) 2022-01-25
US12012626B2 (en) 2024-06-18
JP2021530228A (en) 2021-11-11
KR102532079B1 (en) 2023-05-12
EP3824093A2 (en) 2021-05-26
US20200087692A1 (en) 2020-03-19
JP7305087B2 (en) 2023-07-10
WO2020018506A3 (en) 2020-03-12
MX2021000605A (en) 2021-06-23
US20220162658A1 (en) 2022-05-26
KR20210066790A (en) 2021-06-07

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