MX2015004649A - Metodos de modificacion de celulas huesped. - Google Patents

Metodos de modificacion de celulas huesped.

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Publication number
MX2015004649A
MX2015004649A MX2015004649A MX2015004649A MX2015004649A MX 2015004649 A MX2015004649 A MX 2015004649A MX 2015004649 A MX2015004649 A MX 2015004649A MX 2015004649 A MX2015004649 A MX 2015004649A MX 2015004649 A MX2015004649 A MX 2015004649A
Authority
MX
Mexico
Prior art keywords
methods
host cell
cell modification
host cells
nucleic acid
Prior art date
Application number
MX2015004649A
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English (en)
Other versions
MX365435B (es
Inventor
Michael Wacker
Michael Kowarik
Fabiana Fernandez
Original Assignee
Glycovaxyn Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycovaxyn Ag filed Critical Glycovaxyn Ag
Publication of MX2015004649A publication Critical patent/MX2015004649A/es
Publication of MX365435B publication Critical patent/MX365435B/es

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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/40—Systems of functionally co-operating vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a métodos novedosos de inserción de secuencias de ácido nucleico en células huésped. En la presente también se describen células huésped genéticamente estables que comprenden secuencias de ácido nucleico insertadas, y métodos para utilizar tales células huésped en la generación de proteínas.
MX2015004649A 2012-10-12 2013-10-11 Metodos de modificacion de celulas huesped. MX365435B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261713281P 2012-10-12 2012-10-12
PCT/EP2013/071328 WO2014057109A1 (en) 2012-10-12 2013-10-11 Methods of host cell modification

Publications (2)

Publication Number Publication Date
MX2015004649A true MX2015004649A (es) 2016-01-12
MX365435B MX365435B (es) 2019-06-03

Family

ID=49326691

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015004649A MX365435B (es) 2012-10-12 2013-10-11 Metodos de modificacion de celulas huesped.

Country Status (16)

Country Link
US (2) US20150267207A1 (es)
EP (2) EP2906681B1 (es)
JP (1) JP6329554B2 (es)
KR (1) KR20150068998A (es)
CN (2) CN104837984A (es)
AU (1) AU2013328615B2 (es)
BR (1) BR112015008052A2 (es)
CA (1) CA2887133C (es)
EA (1) EA201590488A1 (es)
ES (2) ES2905107T3 (es)
IL (1) IL238024A0 (es)
MX (1) MX365435B (es)
SG (2) SG11201502638YA (es)
TR (1) TR201904022T4 (es)
WO (1) WO2014057109A1 (es)
ZA (1) ZA201502433B (es)

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CA2889767C (en) * 2012-11-07 2021-09-21 Glycovaxyn Ag Production of recombinant vaccine in e. coli by enzymatic conjugation
US9849169B2 (en) 2013-01-17 2017-12-26 Arsanis Biosciences Gmbh MDR E. coli specific antibody
KR20160021075A (ko) * 2013-03-14 2016-02-24 글리코비아, 인크. 올리고사카라이드 조성물, 당단백질, 및 원핵생물에서의 이들의 제조 방법
SG11201602546RA (en) 2013-10-11 2016-04-28 Glycovaxyn Ag Methods of host cell modification
SG11201606889PA (en) 2014-02-24 2016-09-29 Glycovaxyn Ag Novel polysaccharide and uses thereof
ES2939470T3 (es) * 2014-04-17 2023-04-24 Glaxosmithkline Biologicals Sa Células hospedadoras modificadas y usos de las mismas
CN107429241A (zh) * 2014-08-14 2017-12-01 北京百奥赛图基因生物技术有限公司 Dna敲入系统
JP6764872B2 (ja) 2015-02-26 2020-10-07 ヴァックスニューモ エルエルシーＶａｘｎｅｗｍｏ Ｌｌｃ アシネトバクター（Ａｃｉｎｅｔｏｂａｃｔｅｒ）Ｏ−オリゴサッカリルトランスフェラーゼおよびその使用
TWI715617B (zh) 2015-08-24 2021-01-11 比利時商葛蘭素史密斯克藍生物品公司 對抗腸道外病原性大腸桿菌之免疫保護之方法及組合物
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CA3134045A1 (en) 2019-03-18 2020-09-24 Janssen Pharmaceuticals, Inc. Methods of producing bioconjugates of e. coli o-antigen polysaccharides, compositions thereof, and methods of use thereof
PE20212265A1 (es) 2019-03-18 2021-11-30 Janssen Pharmaceuticals Inc Bioconjugados de antigenos-polisacaridos de e. coli, metodos de produccion y metodos de utilizacion de los mismos
EP3770269A1 (en) 2019-07-23 2021-01-27 GlaxoSmithKline Biologicals S.A. Quantification of bioconjugate glycosylation
WO2021255684A1 (en) * 2020-06-18 2021-12-23 Glaxosmithkline Biologicals Sa Shigella-tetravalent (shigella4v) bioconjugate
CN116209753A (zh) 2020-06-25 2023-06-02 葛兰素史克生物有限公司 肺炎克雷伯氏菌o-抗原疫苗
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Also Published As

Publication number Publication date
JP6329554B2 (ja) 2018-05-23
EP3527663B1 (en) 2021-12-01
AU2013328615A1 (en) 2015-04-30
WO2014057109A1 (en) 2014-04-17
BR112015008052A2 (pt) 2017-11-28
EA201590488A1 (ru) 2015-09-30
CN104837984A (zh) 2015-08-12
CN110423716A (zh) 2019-11-08
ES2720040T3 (es) 2019-07-17
US20150267207A1 (en) 2015-09-24
KR20150068998A (ko) 2015-06-22
US20190218561A1 (en) 2019-07-18
ES2905107T3 (es) 2022-04-07
EP2906681B1 (en) 2019-01-09
CA2887133C (en) 2022-05-03
EP2906681A1 (en) 2015-08-19
MX365435B (es) 2019-06-03
SG11201502638YA (en) 2015-05-28
CA2887133A1 (en) 2014-04-17
IL238024A0 (en) 2015-05-31
JP2015533497A (ja) 2015-11-26
EP3527663A1 (en) 2019-08-21
TR201904022T4 (tr) 2019-04-22
ZA201502433B (en) 2017-11-29
SG10201800777PA (en) 2018-02-27
AU2013328615B2 (en) 2017-04-20

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