MX2014000920A - Metodo para evaluar la integridad de la pared celular bacteriana. - Google Patents

Metodo para evaluar la integridad de la pared celular bacteriana.

Info

Publication number
MX2014000920A
MX2014000920A MX2014000920A MX2014000920A MX2014000920A MX 2014000920 A MX2014000920 A MX 2014000920A MX 2014000920 A MX2014000920 A MX 2014000920A MX 2014000920 A MX2014000920 A MX 2014000920A MX 2014000920 A MX2014000920 A MX 2014000920A
Authority
MX
Mexico
Prior art keywords
cell wall
bacterial cell
antibiotic
bacteria
wall integrity
Prior art date
Application number
MX2014000920A
Other languages
English (en)
Other versions
MX357457B (es
Inventor
Berenguer Jaime Gosálvez
García José Luis Fernández
Arévalo Germán Bou
Novas María Tamayo
Brandariz Rebeca Santis
Original Assignee
Univ Autónoma De Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Autónoma De Madrid filed Critical Univ Autónoma De Madrid
Publication of MX2014000920A publication Critical patent/MX2014000920A/es
Publication of MX357457B publication Critical patent/MX357457B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se relaciona con un método para evaluar la integridad de la pared celular de las bacterias presentes en un cultivo en presencia de un antibiótico que actúa a nivel de la pared celular bacteriana, que desde un punto vista práctico, permite determinar de forma rápida si una bacteria es sensible o resistente a un antibiótico que actúa a nivel de la pared celular bacteriana. Asimismo, la presente invención también se relaciona con una solución de lisis de aplicación en el método anterior, que afecta de forma específica a las bacterias que presentan la pared celular dañada por la acción de un antibiótico que actúa a nivel de la pared celular bacteriana, permitiendo distinguir las bacterias sensibles de las resistentes a dicho antibiótico.
MX2014000920A 2011-07-26 2012-07-26 Metodo para evaluar la integridad de la pared celular bacteriana. MX357457B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES201131276A ES2396820B1 (es) 2011-07-26 2011-07-26 Método para evaluar la integridad de la pared celular bacteriana.
PCT/ES2012/070575 WO2013014324A1 (es) 2011-07-26 2012-07-26 Método para evaluar la integridad de la pared celular bacteriana

Publications (2)

Publication Number Publication Date
MX2014000920A true MX2014000920A (es) 2014-05-12
MX357457B MX357457B (es) 2018-07-11

Family

ID=47600556

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000920A MX357457B (es) 2011-07-26 2012-07-26 Metodo para evaluar la integridad de la pared celular bacteriana.

Country Status (9)

Country Link
US (1) US9976170B2 (es)
EP (2) EP4050108A1 (es)
CN (1) CN103946389A (es)
BR (1) BR112014001871B1 (es)
CA (1) CA2842865C (es)
ES (2) ES2396820B1 (es)
HK (1) HK1197271A1 (es)
MX (1) MX357457B (es)
WO (1) WO2013014324A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2821499B1 (en) 2013-07-04 2017-02-15 ABM Technologies, LLC Method for the rapid determination of susceptibility or resistance of bacteria to antibiotics
WO2015048696A2 (en) 2013-09-30 2015-04-02 Advandx, Inc. Antimicrobial compound susceptibility test
US9597319B2 (en) * 2014-04-23 2017-03-21 Case Western Reserve University Compositions and methods of inhibiting metallo-β-lactamases
ES2710679T3 (es) 2015-01-21 2019-04-26 Abm Tech Llc Procedimiento para la determinación rápida de la susceptibilidad bacteriana a los antibióticos que inhiben la síntesis de las proteínas
CN107656056B (zh) * 2017-08-29 2019-05-28 山东师范大学 一种基于细菌增长对细菌快速镜检的方法
GB201801022D0 (en) * 2018-01-22 2018-03-07 Q Linea Ab Single dye concentration determination
FR3103197A1 (fr) * 2019-11-15 2021-05-21 bioMérieux Determination par spectrometrie de masse de la sensibilite ou de la resistance de bacteries a un antibiotique
CN111458313A (zh) * 2020-04-07 2020-07-28 上海交通大学医学院附属仁济医院 基于荧光d型氨基酸代谢标记的抗菌药敏试验检测方法
CN113188876B (zh) * 2021-04-29 2022-10-25 西北农林科技大学 一种枣根尖染色体压片的制备方法
CN113106082B (zh) * 2021-05-27 2022-11-04 云南师范大学 动物粪便宏基因组来源的丙氨酸消旋酶及其制备和应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2128737B (en) * 1982-10-07 1986-02-05 Beecham Group Plc Antibiotic susceptibility test material
DE3327839A1 (de) 1983-08-02 1985-02-14 Merck Patent Gmbh, 6100 Darmstadt Verfahren und mittel zur empfindlichkeitspruefung von bakterien
JPH0787800B2 (ja) 1991-05-06 1995-09-27 バクスター、ダイアグノスチックス、インコーポレイテッド 迅速誘導可能β―ラクタマーゼスクリーン試験
JP3636849B2 (ja) * 1996-11-14 2005-04-06 キッコーマン株式会社 微生物の薬剤感受性試験法、同試験用キット、微生物の最少発育阻止濃度測定法並びに同測定用キット
GB0013384D0 (en) 2000-06-01 2000-07-26 Plant Bioscience Ltd Screening system
ATE431425T1 (de) * 2000-09-28 2009-05-15 Nakane Diagnostics Inc Schnelltest der antibiotika-empfindlichkeit
SE0104102D0 (sv) * 2001-12-05 2001-12-05 Astrazeneca Ab New assay
US7993827B2 (en) * 2004-01-26 2011-08-09 Universidad Autonoma De Madrid Procedure for the determination of fragmentation of DNA in animal sperm
US20110151455A1 (en) * 2006-06-23 2011-06-23 University Of South Florida Fish-ribosyn for antibiotic susceptibility testing
ES2329637B1 (es) * 2006-11-10 2010-09-22 Universidad Autonoma De Madrid Procedimiento para la determinacion de la fragmentacion del adn en microorganismos.
US20080176320A1 (en) * 2007-01-16 2008-07-24 Applera Corporation Selective Lysis of Sperm Cells
KR20090058221A (ko) * 2007-12-04 2009-06-09 삼성전자주식회사 시료 중의 세포 집단으로부터 죽은 세포를 선택적으로파쇄하는 방법
EP2333105A1 (en) * 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells

Also Published As

Publication number Publication date
EP2738262A1 (en) 2014-06-04
EP4050108A1 (en) 2022-08-31
WO2013014324A1 (es) 2013-01-31
BR112014001871B1 (pt) 2021-03-30
CA2842865A1 (en) 2013-01-31
EP2738262A4 (en) 2015-04-08
ES2908261T3 (es) 2022-04-28
ES2396820B1 (es) 2014-01-31
MX357457B (es) 2018-07-11
US20140206573A1 (en) 2014-07-24
HK1197271A1 (en) 2015-01-09
US9976170B2 (en) 2018-05-22
CN103946389A (zh) 2014-07-23
EP2738262B1 (en) 2022-02-09
BR112014001871A2 (pt) 2017-02-21
CA2842865C (en) 2020-03-10
ES2396820A1 (es) 2013-02-27

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