MX357457B - Metodo para evaluar la integridad de la pared celular bacteriana. - Google Patents

Metodo para evaluar la integridad de la pared celular bacteriana.

Info

Publication number
MX357457B
MX357457B MX2014000920A MX2014000920A MX357457B MX 357457 B MX357457 B MX 357457B MX 2014000920 A MX2014000920 A MX 2014000920A MX 2014000920 A MX2014000920 A MX 2014000920A MX 357457 B MX357457 B MX 357457B
Authority
MX
Mexico
Prior art keywords
cell wall
bacterial cell
antibiotic
bacteria
wall integrity
Prior art date
Application number
MX2014000920A
Other languages
English (en)
Other versions
MX2014000920A (es
Inventor
Berenguer Jaime Gosálvez
García José Luis Fernández
Arévalo Germán Bou
Novas María Tamayo
Santiso Brandariz Rebeca
Original Assignee
Univ Madrid Autonoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Madrid Autonoma filed Critical Univ Madrid Autonoma
Publication of MX2014000920A publication Critical patent/MX2014000920A/es
Publication of MX357457B publication Critical patent/MX357457B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para evaluar la integridad de la pared celular de las bacterias presentes en un cultivo en presencia de un antibiótico que actúa a nivel de la pared celular bacteriana, que desde un punto vista práctico, permite determinar de forma rápida si una bacteria es sensible o resistente a un antibiótico que actúa a nivel de la pared celular bacteriana. Asimismo, la presente invención también se relaciona con una solución de lisis de aplicación en el método anterior, que afecta de forma específica a las bacterias que presentan la pared celular dañada por la acción de un antibiótico que actúa a nivel de la pared celular bacteriana, permitiendo distinguir las bacterias sensibles de las resistentes a dicho antibiótico.
MX2014000920A 2011-07-26 2012-07-26 Metodo para evaluar la integridad de la pared celular bacteriana. MX357457B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES201131276A ES2396820B1 (es) 2011-07-26 2011-07-26 Método para evaluar la integridad de la pared celular bacteriana.
PCT/ES2012/070575 WO2013014324A1 (es) 2011-07-26 2012-07-26 Método para evaluar la integridad de la pared celular bacteriana

Publications (2)

Publication Number Publication Date
MX2014000920A MX2014000920A (es) 2014-05-12
MX357457B true MX357457B (es) 2018-07-11

Family

ID=47600556

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000920A MX357457B (es) 2011-07-26 2012-07-26 Metodo para evaluar la integridad de la pared celular bacteriana.

Country Status (9)

Country Link
US (1) US9976170B2 (es)
EP (2) EP2738262B1 (es)
CN (1) CN103946389A (es)
BR (1) BR112014001871B1 (es)
CA (1) CA2842865C (es)
ES (2) ES2396820B1 (es)
HK (1) HK1197271A1 (es)
MX (1) MX357457B (es)
WO (1) WO2013014324A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
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EP2821499B1 (en) * 2013-07-04 2017-02-15 ABM Technologies, LLC Method for the rapid determination of susceptibility or resistance of bacteria to antibiotics
EP3052642A2 (en) 2013-09-30 2016-08-10 AdvanDx, Inc. Antimicrobial compound susceptibility test
US9597319B2 (en) * 2014-04-23 2017-03-21 Case Western Reserve University Compositions and methods of inhibiting metallo-β-lactamases
ES2710679T3 (es) 2015-01-21 2019-04-26 Abm Tech Llc Procedimiento para la determinación rápida de la susceptibilidad bacteriana a los antibióticos que inhiben la síntesis de las proteínas
CN107656056B (zh) * 2017-08-29 2019-05-28 山东师范大学 一种基于细菌增长对细菌快速镜检的方法
GB201716883D0 (en) 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
GB201801022D0 (en) * 2018-01-22 2018-03-07 Q Linea Ab Single dye concentration determination
CA3139767C (en) * 2019-05-31 2024-10-15 Ian Andrew LEWIS METABOMIC CHARACTERIZATION OF MICROORGANISMS
FR3103197B1 (fr) 2019-11-15 2024-08-30 Biomerieux Sa Determination par spectrometrie de masse de la sensibilite ou de la resistance de bacteries a un antibiotique
CN111458313A (zh) * 2020-04-07 2020-07-28 上海交通大学医学院附属仁济医院 基于荧光d型氨基酸代谢标记的抗菌药敏试验检测方法
CN113188876B (zh) * 2021-04-29 2022-10-25 西北农林科技大学 一种枣根尖染色体压片的制备方法
CN113106082B (zh) * 2021-05-27 2022-11-04 云南师范大学 动物粪便宏基因组来源的丙氨酸消旋酶及其制备和应用
CN118222733B (zh) * 2024-04-07 2025-06-27 杭州迪安医学检验中心有限公司 结核分枝杆菌的pcr检测组合物及其舌拭子检测试剂盒

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
GB2128737B (en) * 1982-10-07 1986-02-05 Beecham Group Plc Antibiotic susceptibility test material
DE3327839A1 (de) 1983-08-02 1985-02-14 Merck Patent Gmbh, 6100 Darmstadt Verfahren und mittel zur empfindlichkeitspruefung von bakterien
JPH0787800B2 (ja) 1991-05-06 1995-09-27 バクスター、ダイアグノスチックス、インコーポレイテッド 迅速誘導可能β―ラクタマーゼスクリーン試験
JP3636849B2 (ja) * 1996-11-14 2005-04-06 キッコーマン株式会社 微生物の薬剤感受性試験法、同試験用キット、微生物の最少発育阻止濃度測定法並びに同測定用キット
GB0013384D0 (en) 2000-06-01 2000-07-26 Plant Bioscience Ltd Screening system
WO2002055015A2 (en) * 2000-09-28 2002-07-18 Essential Therapeutics, Inc. Rapid antibiotic susceptibility test
SE0104102D0 (sv) * 2001-12-05 2001-12-05 Astrazeneca Ab New assay
KR20070090073A (ko) * 2004-01-26 2007-09-05 유니버시다드 오토노마 데 마드리드 동물 정자에서 ｄｎａ의 단편화 결정을 위한 절차
US20110151455A1 (en) * 2006-06-23 2011-06-23 University Of South Florida Fish-ribosyn for antibiotic susceptibility testing
ES2329637B1 (es) * 2006-11-10 2010-09-22 Universidad Autonoma De Madrid Procedimiento para la determinacion de la fragmentacion del adn en microorganismos.
WO2008089280A2 (en) * 2007-01-16 2008-07-24 Applied Biosystems, Llc Selective lysis of sperm cells
KR20090058221A (ko) * 2007-12-04 2009-06-09 삼성전자주식회사 시료 중의 세포 집단으로부터 죽은 세포를 선택적으로파쇄하는 방법
EP2333105A1 (en) * 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells

Also Published As

Publication number Publication date
EP4050108A1 (en) 2022-08-31
CN103946389A (zh) 2014-07-23
EP2738262A4 (en) 2015-04-08
ES2396820A1 (es) 2013-02-27
CA2842865C (en) 2020-03-10
BR112014001871A2 (pt) 2017-02-21
BR112014001871B1 (pt) 2021-03-30
WO2013014324A1 (es) 2013-01-31
US9976170B2 (en) 2018-05-22
EP2738262B1 (en) 2022-02-09
MX2014000920A (es) 2014-05-12
US20140206573A1 (en) 2014-07-24
EP2738262A1 (en) 2014-06-04
ES2908261T3 (es) 2022-04-28
HK1197271A1 (en) 2015-01-09
ES2396820B1 (es) 2014-01-31
CA2842865A1 (en) 2013-01-31

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