LU85526A1 - Reactif pour la determination du nombre des thrombocytes et des leucocytes - Google Patents
Reactif pour la determination du nombre des thrombocytes et des leucocytes Download PDFInfo
- Publication number
- LU85526A1 LU85526A1 LU85526A LU85526A LU85526A1 LU 85526 A1 LU85526 A1 LU 85526A1 LU 85526 A LU85526 A LU 85526A LU 85526 A LU85526 A LU 85526A LU 85526 A1 LU85526 A1 LU 85526A1
- Authority
- LU
- Luxembourg
- Prior art keywords
- parts
- weight
- thrombocytes
- leukocytes
- solution
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 16
- 210000001772 blood platelet Anatomy 0.000 claims description 42
- 210000000265 leukocyte Anatomy 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 25
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 229950003937 tolonium Drugs 0.000 claims description 7
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000001016 thiazine dye Substances 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 3
- 239000011707 mineral Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000001045 blue dye Substances 0.000 claims description 2
- 238000005342 ion exchange Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 description 31
- 210000004369 blood Anatomy 0.000 description 15
- 239000008280 blood Substances 0.000 description 15
- 239000000975 dye Substances 0.000 description 10
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 229960001484 edetic acid Drugs 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 239000003146 anticoagulant agent Substances 0.000 description 4
- 229940127219 anticoagulant drug Drugs 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 description 2
- 239000006103 coloring component Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- ZXJXZNDDNMQXFV-UHFFFAOYSA-M crystal violet Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1[C+](C=1C=CC(=CC=1)N(C)C)C1=CC=C(N(C)C)C=C1 ZXJXZNDDNMQXFV-UHFFFAOYSA-M 0.000 description 2
- 229960000587 glutaral Drugs 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 2
- 230000014508 negative regulation of coagulation Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- PVPBBTJXIKFICP-UHFFFAOYSA-N (7-aminophenothiazin-3-ylidene)azanium;chloride Chemical compound [Cl-].C1=CC(=[NH2+])C=C2SC3=CC(N)=CC=C3N=C21 PVPBBTJXIKFICP-UHFFFAOYSA-N 0.000 description 1
- AGIJRRREJXSQJR-UHFFFAOYSA-N 2h-thiazine Chemical compound N1SC=CC=C1 AGIJRRREJXSQJR-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- ZHAFUINZIZIXFC-UHFFFAOYSA-N [9-(dimethylamino)-10-methylbenzo[a]phenoxazin-5-ylidene]azanium;chloride Chemical compound [Cl-].O1C2=CC(=[NH2+])C3=CC=CC=C3C2=NC2=C1C=C(N(C)C)C(C)=C2 ZHAFUINZIZIXFC-UHFFFAOYSA-N 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 229960003920 cocaine Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960001235 gentian violet Drugs 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002489 hematologic effect Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000012886 linear function Methods 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229940053973 novocaine Drugs 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- MFDFERRIHVXMIY-UHFFFAOYSA-N procaine Chemical compound CCN(CC)CCOC(=O)C1=CC=C(N)C=C1 MFDFERRIHVXMIY-UHFFFAOYSA-N 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Ecology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU306083 | 1983-09-02 | ||
| HU833060A HU186309B (en) | 1983-09-02 | 1983-09-02 | Reagent for determining the thrombacyta and leucocyte number |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LU85526A1 true LU85526A1 (fr) | 1986-03-11 |
Family
ID=10962336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LU85526A LU85526A1 (fr) | 1983-09-02 | 1984-08-31 | Reactif pour la determination du nombre des thrombocytes et des leucocytes |
Country Status (14)
| Country | Link |
|---|---|
| AT (1) | AT390844B (cs) |
| BE (1) | BE900439A (cs) |
| CH (1) | CH665030A5 (cs) |
| CS (1) | CS274409B2 (cs) |
| DD (1) | DD232557A5 (cs) |
| DE (1) | DE3432351A1 (cs) |
| FI (1) | FI77938C (cs) |
| FR (1) | FR2551551B1 (cs) |
| HU (1) | HU186309B (cs) |
| IN (1) | IN162894B (cs) |
| LU (1) | LU85526A1 (cs) |
| NL (1) | NL8402670A (cs) |
| PL (1) | PL142043B1 (cs) |
| SE (1) | SE455235B (cs) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2685482B1 (fr) * | 1991-12-24 | 1994-07-29 | Melet Francois | Procede de numeration des hematies ou des thrombocytes et dispositif de mise en óoeuvre. |
| ES2634715T3 (es) * | 2011-12-30 | 2017-09-28 | Abbott Point Of Care, Inc | Método y aparato para la identificación automatizada de plaquetas en una muestra de sangre completa a partir de imágenes de microscopio |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3916205A (en) * | 1973-05-31 | 1975-10-28 | Block Engineering | Differential counting of leukocytes and other cells |
| CH613523A5 (en) * | 1975-06-27 | 1979-09-28 | Inst Nat Sante Rech Med | Method for displaying basophils |
| US4290769A (en) * | 1980-10-14 | 1981-09-22 | Miles Laboratories, Inc. | Stabilized Romanowsky stain solution |
| US4392864A (en) * | 1982-02-01 | 1983-07-12 | Miles Laboratories, Inc. | Stabilized Romanowsky stain solution |
-
1983
- 1983-09-02 HU HU833060A patent/HU186309B/hu not_active IP Right Cessation
-
1984
- 1984-08-27 CH CH4076/84A patent/CH665030A5/de not_active IP Right Cessation
- 1984-08-28 BE BE1/11080A patent/BE900439A/fr not_active IP Right Cessation
- 1984-08-30 FR FR8413421A patent/FR2551551B1/fr not_active Expired
- 1984-08-31 AT AT0279984A patent/AT390844B/de not_active IP Right Cessation
- 1984-08-31 FI FI843429A patent/FI77938C/fi not_active IP Right Cessation
- 1984-08-31 SE SE8404355A patent/SE455235B/sv not_active IP Right Cessation
- 1984-08-31 NL NL8402670A patent/NL8402670A/nl not_active Application Discontinuation
- 1984-08-31 PL PL1984249427A patent/PL142043B1/pl unknown
- 1984-08-31 DD DD84266855A patent/DD232557A5/de not_active IP Right Cessation
- 1984-08-31 LU LU85526A patent/LU85526A1/fr unknown
- 1984-08-31 CS CS658184A patent/CS274409B2/cs unknown
- 1984-09-03 DE DE19843432351 patent/DE3432351A1/de not_active Withdrawn
-
1985
- 1985-02-18 IN IN135/DEL/85A patent/IN162894B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS274409B2 (en) | 1991-04-11 |
| ATA279984A (de) | 1989-12-15 |
| FI843429A0 (fi) | 1984-08-31 |
| FR2551551A1 (fr) | 1985-03-08 |
| CS658184A2 (en) | 1990-09-12 |
| IN162894B (cs) | 1988-07-16 |
| PL249427A1 (en) | 1985-07-30 |
| FI77938C (fi) | 1989-05-10 |
| SE8404355L (sv) | 1985-03-03 |
| SE455235B (sv) | 1988-06-27 |
| FI843429L (fi) | 1985-03-03 |
| FR2551551B1 (fr) | 1987-04-24 |
| DE3432351A1 (de) | 1985-03-21 |
| HU186309B (en) | 1985-07-29 |
| DD232557A5 (de) | 1986-01-29 |
| AT390844B (de) | 1990-07-10 |
| PL142043B1 (en) | 1987-09-30 |
| FI77938B (fi) | 1989-01-31 |
| BE900439A (fr) | 1985-02-28 |
| SE8404355D0 (sv) | 1984-08-31 |
| NL8402670A (nl) | 1985-04-01 |
| CH665030A5 (de) | 1988-04-15 |
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