CH613523A5 - Method for displaying basophils - Google Patents
Method for displaying basophilsInfo
- Publication number
- CH613523A5 CH613523A5 CH787076A CH787076A CH613523A5 CH 613523 A5 CH613523 A5 CH 613523A5 CH 787076 A CH787076 A CH 787076A CH 787076 A CH787076 A CH 787076A CH 613523 A5 CH613523 A5 CH 613523A5
- Authority
- CH
- Switzerland
- Prior art keywords
- basophils
- displaying
- metachromatic
- composition
- allergies
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 210000003651 basophil Anatomy 0.000 title abstract 2
- 230000003458 metachromatic effect Effects 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 206010020751 Hypersensitivity Diseases 0.000 abstract 1
- 230000007815 allergy Effects 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 229950003937 tolonium Drugs 0.000 abstract 1
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Ecology (AREA)
- Physiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a method and a metachromatic composition for counting leucocytes, more particularly basophils. The metachromatic composition used in the method comprises a water/alcohol solution of a toluidine blue acidified so as to exhibit a pH of approximately 3 to 5. Application: diagnosis of allergies. <IMAGE>
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7520273A FR2315251A1 (en) | 1975-06-27 | 1975-06-27 | Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276) |
FR7609146A FR2346717A2 (en) | 1976-03-30 | 1976-03-30 | Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276) |
Publications (1)
Publication Number | Publication Date |
---|---|
CH613523A5 true CH613523A5 (en) | 1979-09-28 |
Family
ID=26218951
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CH787076A CH613523A5 (en) | 1975-06-27 | 1976-06-21 | Method for displaying basophils |
Country Status (8)
Country | Link |
---|---|
JP (1) | JPS5819063B2 (en) |
CA (1) | CA1087966A (en) |
CH (1) | CH613523A5 (en) |
DE (1) | DE2628468C2 (en) |
DK (1) | DK289076A (en) |
GB (1) | GB1560729A (en) |
NL (1) | NL171840C (en) |
SE (1) | SE437079B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0177352A1 (en) * | 1984-10-04 | 1986-04-09 | The State Of Victoria | Improvements in or relating to enzyme-linked immunosorbent assay |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0022698B1 (en) * | 1979-06-28 | 1986-08-13 | Institut Pasteur | Reagent for detecting parasitoses and allergies, detection process using this reagent and test kit therefor |
US4321251A (en) * | 1979-12-19 | 1982-03-23 | The United States Of America As Represented By The Department Of Health And Human Services | Detection of malignant lesions of the oral cavity utilizing toluidine blue rinse |
US4840784A (en) * | 1982-12-22 | 1989-06-20 | Eastman Kodak Company | Use of pyrylium and thiapyrylium compounds as biological stains |
US4555396A (en) * | 1982-12-22 | 1985-11-26 | Eastman Kodak Company | Use of pyrylium and thiapyrylium compounds as biological stains |
SE461660B (en) * | 1983-04-19 | 1990-03-12 | Bio Instructa Labkonsult | PROCEDURE FOR ANALYSIS OF RECEPTOR ACTIVITIES ON CELLS |
HU186309B (en) * | 1983-09-02 | 1985-07-29 | Reanal Finomvegyszergyar | Reagent for determining the thrombacyta and leucocyte number |
JPS62135752U (en) * | 1986-02-20 | 1987-08-26 | ||
JPH0448708Y2 (en) * | 1986-04-10 | 1992-11-17 | ||
CA1309327C (en) * | 1986-09-10 | 1992-10-27 | Tomoyuki Kuroda | Reagent and method for classifying leukocytes by flow cytometry |
US5179026A (en) * | 1986-11-27 | 1993-01-12 | Toa Medical Electronics Co., Ltd. | Method of classifying leukocytes by flow cytometry and reagents used in the method |
US5039613A (en) * | 1986-11-27 | 1991-08-13 | Toa Medical Electronics Co., Ltd. | Reagents used in a method of classifying leukocytes by flow cytometry |
US5155044A (en) * | 1987-03-13 | 1992-10-13 | Coulter Electronics, Inc. | Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples |
IL85532A (en) * | 1987-03-13 | 1992-03-29 | Coulter Electronics | Method and lytic reagent system for isolation,identification and/or analysis of leukocytes from whole blood samples |
PT106082A (en) * | 2012-01-04 | 2013-07-04 | Fernando Jorge Neves Ferreira | FIXING COMPOSITION FOR CYTOLOGY, CELL FITTING METHOD AND ITS APPLICATIONS |
US20240036066A1 (en) * | 2020-12-22 | 2024-02-01 | Radiometer Medical Aps | Method for monitoring and adjusting reagent for hematology |
-
1976
- 1976-06-21 CH CH787076A patent/CH613523A5/en not_active IP Right Cessation
- 1976-06-24 SE SE7607282A patent/SE437079B/en not_active IP Right Cessation
- 1976-06-25 CA CA255,681A patent/CA1087966A/en not_active Expired
- 1976-06-25 DK DK289076A patent/DK289076A/en not_active Application Discontinuation
- 1976-06-25 DE DE19762628468 patent/DE2628468C2/en not_active Expired
- 1976-06-25 NL NL7607012A patent/NL171840C/en not_active IP Right Cessation
- 1976-06-28 JP JP51076303A patent/JPS5819063B2/en not_active Expired
- 1976-06-28 GB GB2676876A patent/GB1560729A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0177352A1 (en) * | 1984-10-04 | 1986-04-09 | The State Of Victoria | Improvements in or relating to enzyme-linked immunosorbent assay |
Also Published As
Publication number | Publication date |
---|---|
CA1087966A (en) | 1980-10-21 |
GB1560729A (en) | 1980-02-06 |
NL171840C (en) | 1983-05-16 |
DE2628468C2 (en) | 1982-05-06 |
JPS5224589A (en) | 1977-02-24 |
JPS5819063B2 (en) | 1983-04-15 |
SE7607282L (en) | 1976-12-28 |
DE2628468A1 (en) | 1976-12-30 |
SE437079B (en) | 1985-02-04 |
DK289076A (en) | 1976-12-28 |
NL7607012A (en) | 1976-12-29 |
NL171840B (en) | 1982-12-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PL | Patent ceased |