FR2346717A2 - Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276) - Google Patents

Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Info

Publication number
FR2346717A2
FR2346717A2 FR7609146A FR7609146A FR2346717A2 FR 2346717 A2 FR2346717 A2 FR 2346717A2 FR 7609146 A FR7609146 A FR 7609146A FR 7609146 A FR7609146 A FR 7609146A FR 2346717 A2 FR2346717 A2 FR 2346717A2
Authority
FR
France
Prior art keywords
basophils
counting
acid
reagent
metachromatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7609146A
Other languages
French (fr)
Other versions
FR2346717B2 (en
Inventor
J Benveniste
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Priority to FR7609146A priority Critical patent/FR2346717A2/en
Priority to CH787076A priority patent/CH613523A5/en
Priority to SE7607282A priority patent/SE437079B/en
Priority to DK289076A priority patent/DK289076A/en
Priority to CA255,681A priority patent/CA1087966A/en
Priority to NL7607012A priority patent/NL171840C/en
Priority to DE19762628468 priority patent/DE2628468C2/en
Priority to GB2676876A priority patent/GB1560729A/en
Priority to JP51076303A priority patent/JPS5819063B2/en
Priority to ES454579A priority patent/ES454579A1/en
Publication of FR2346717A2 publication Critical patent/FR2346717A2/en
Application granted granted Critical
Publication of FR2346717B2 publication Critical patent/FR2346717B2/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Ecology (AREA)
  • Physiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Novel reagent for counting basophils and opt. other leukocytes in a biological medium (esp. a blood sample) consists of a hypotonic aq. soln. contg. a metachromatic reagent (pref. toluidine blue), a fixative for leukocytes and basophils, and an acid capable of contributing to the disruption of erythrocytes in blood samples. The acid is present in such an amt. that the final pH of the reagent is 3-5. The process comprises directly mixing the sample, pref. in a single step, with the above reagent. Visualisation and counting of basophils is used in a variety of diagnostic procedures, pref. for the diagnosis of anaphylactic sensitivities, esp. allergies, involving incubating several fractions of a blood sample with 1 agent (esp. an allergen) having a degranulating effect on basophils.
FR7609146A 1975-06-27 1976-03-30 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276) Granted FR2346717A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR7609146A FR2346717A2 (en) 1976-03-30 1976-03-30 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)
CH787076A CH613523A5 (en) 1975-06-27 1976-06-21 Method for displaying basophils
SE7607282A SE437079B (en) 1975-06-27 1976-06-24 SET TO VISUALIZE BASOFILES, REAGENTS FOR VISUALIZING BASOFILES, AND USE OF THE SET AND REAGENTS
CA255,681A CA1087966A (en) 1975-06-27 1976-06-25 Process and a metachromatic composition for effecting a count of leucocytes, and more particularly of basophiles
DK289076A DK289076A (en) 1975-06-27 1976-06-25 PROCEDURE AND COLOR CHANGE FOR LISTING LEUCOCYTS ISER BASOPHILE BLOOD BODIES
NL7607012A NL171840C (en) 1975-06-27 1976-06-25 METHOD FOR PREPARING A REAGENT FOR COUNTING BASOFIELS.
DE19762628468 DE2628468C2 (en) 1975-06-27 1976-06-25 Method for staining basophils and reagent for carrying out this method
GB2676876A GB1560729A (en) 1975-06-27 1976-06-28 Counting of leucocytes
JP51076303A JPS5819063B2 (en) 1975-06-27 1976-06-28 Reagent for counting leukocytes, especially basophil cells, and calculation method using the reagent
ES454579A ES454579A1 (en) 1976-03-30 1976-12-24 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7609146A FR2346717A2 (en) 1976-03-30 1976-03-30 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Publications (2)

Publication Number Publication Date
FR2346717A2 true FR2346717A2 (en) 1977-10-28
FR2346717B2 FR2346717B2 (en) 1982-02-19

Family

ID=9171123

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7609146A Granted FR2346717A2 (en) 1975-06-27 1976-03-30 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Country Status (2)

Country Link
ES (1) ES454579A1 (en)
FR (1) FR2346717A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2467401A1 (en) * 1979-10-12 1981-04-17 Weck Alain De Measuring de-granulation of basophiles by flow cytometry - after staining leucocytes with Alcian blue, useful in diagnosing allergies
FR2530028A2 (en) * 1982-07-09 1984-01-13 Pasteur Institut NOVEL REAGENT FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES, PROCESS FOR THE PREPARATION OF THIS NEW REAGENT AND METHOD FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES USING SAID REAGENT

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2467401A1 (en) * 1979-10-12 1981-04-17 Weck Alain De Measuring de-granulation of basophiles by flow cytometry - after staining leucocytes with Alcian blue, useful in diagnosing allergies
FR2530028A2 (en) * 1982-07-09 1984-01-13 Pasteur Institut NOVEL REAGENT FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES, PROCESS FOR THE PREPARATION OF THIS NEW REAGENT AND METHOD FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES USING SAID REAGENT
EP0099812A1 (en) * 1982-07-09 1984-02-01 Institut Pasteur Reagent for the diagnosis of parasitosis and allergies, method for the preparation of this reagent and method of diagnosing parasitosis and allergies using this reagent
WO1984000420A1 (en) * 1982-07-09 1984-02-02 Pasteur Institut New reagent for the diagnostic of parasitosis and allergia, process for the preparation of this new reagent and process for the diagnostic of parasitosis and allergia by means of said reagent

Also Published As

Publication number Publication date
FR2346717B2 (en) 1982-02-19
ES454579A1 (en) 1978-05-01

Similar Documents

Publication Publication Date Title
TW369599B (en) A reagent strip and a method for measuring glucose concentration in a sample of whole blood
ATE350665T1 (en) HOMOGENEOUS TEST SYSTEM FOR DETERMINING AN ANALYTE
ATE120543T1 (en) DEVICE AND METHOD FOR ACCURATE, QUICK AND EASY PERFORMANCE OF A PROTHROMBIN TEST.
DE3485653D1 (en) METHOD AND DEVICE FOR MEASURING THE DEGREE OF REACTION BETWEEN ANTIGEN AND CELL LEUCOCYTE ANTIBODIES.
IE40749B1 (en) Test system device with identification code
IE44238L (en) Device for diagnosis of pregnancy
SE7607282L (en) METHODS AND METACHROMIC COMPOSITION FOR DETERMINATION OF LEUCOCYTS, SPECIAL BASOFILES
CA2235316A1 (en) A method for detecting antipolymer antibodies and a diagnostic test kit for use in aiding the diagnosis of silicone related diseases (srd)
FR2315251A1 (en) Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)
US2999052A (en) Composition for colorimetric test for serum enzymes
FR2346717A2 (en) Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)
US3682586A (en) Process for the determination of creatinine body fluids
Seller et al. Polyacrylamide gel electrophoresis of amniotic fluid cholinesterases: a good prenatal test for neural tube defects
EP0303229A3 (en) Method of high sensitivity immunoassay
US3785929A (en) Diagnostic composition for the detection of nitrite
Edrissian et al. Application of enzyme-linked immunosorbent assay (ELISA) to identification of Anopheles mosquito bloodmeals
EP0325723A3 (en) Method for the determination of blood clotting factors and reagent for use therein
ATE15546T1 (en) HAEMOLYTIC PROCEDURE FOR THE KINETIC DETERMINATION OF ANTISTREPTOLYSIN-O ANTIBODIES IN BLOOD OR SERUM SAMPLES USING OXIDIZED STREPTOLYSIN-O.
GB1508133A (en) Biological analysis
FR2319903A1 (en) Automatic chemical and clinical analysis partic. for blood tests - and diagnostic application in bacteriology, rheumatology, virology, allergies etc.
SU1175442A1 (en) Method of diagnosis of brain hypoxia of the newborns
Mischler et al. Immunological identification of human seminal stains
SU1105189A1 (en) Method of diagnosis of atopy
GB1219555A (en) Colorimetric reagent
IT1257967B (en) PROCEDURE AND DEVICE FOR PARTICLE AGGLUTINATION TEST