SE7607282L - METHODS AND METACHROMIC COMPOSITION FOR DETERMINATION OF LEUCOCYTS, SPECIAL BASOFILES - Google Patents

METHODS AND METACHROMIC COMPOSITION FOR DETERMINATION OF LEUCOCYTS, SPECIAL BASOFILES

Info

Publication number
SE7607282L
SE7607282L SE7607282A SE7607282A SE7607282L SE 7607282 L SE7607282 L SE 7607282L SE 7607282 A SE7607282 A SE 7607282A SE 7607282 A SE7607282 A SE 7607282A SE 7607282 L SE7607282 L SE 7607282L
Authority
SE
Sweden
Prior art keywords
leucocyts
basofiles
metachromic
determination
special
Prior art date
Application number
SE7607282A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE437079B (en
Inventor
J Benveniste
Original Assignee
Inst Nat Sante Rech Med
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR7520273A external-priority patent/FR2315251A1/en
Priority claimed from FR7609146A external-priority patent/FR2346717A2/en
Application filed by Inst Nat Sante Rech Med filed Critical Inst Nat Sante Rech Med
Publication of SE7607282L publication Critical patent/SE7607282L/en
Publication of SE437079B publication Critical patent/SE437079B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Ecology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a process for counting in vitro leucocytes, more particularly non degranulated basophils contained in a blood sample which comprises contact of the blood sample to be tested with a reagent containing a metachromate agent, a fixative fox leucocytes and basophils, and an acid present in such proportions that the final pH of the sample/reagent mixture is between about 3 and 5. Said process can be used for diagnosing in vitro anaphylactic sensitivity more particularly allergies.
SE7607282A 1975-06-27 1976-06-24 SET TO VISUALIZE BASOFILES, REAGENTS FOR VISUALIZING BASOFILES, AND USE OF THE SET AND REAGENTS SE437079B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR7520273A FR2315251A1 (en) 1975-06-27 1975-06-27 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)
FR7609146A FR2346717A2 (en) 1976-03-30 1976-03-30 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Publications (2)

Publication Number Publication Date
SE7607282L true SE7607282L (en) 1976-12-28
SE437079B SE437079B (en) 1985-02-04

Family

ID=26218951

Family Applications (1)

Application Number Title Priority Date Filing Date
SE7607282A SE437079B (en) 1975-06-27 1976-06-24 SET TO VISUALIZE BASOFILES, REAGENTS FOR VISUALIZING BASOFILES, AND USE OF THE SET AND REAGENTS

Country Status (8)

Country Link
JP (1) JPS5819063B2 (en)
CA (1) CA1087966A (en)
CH (1) CH613523A5 (en)
DE (1) DE2628468C2 (en)
DK (1) DK289076A (en)
GB (1) GB1560729A (en)
NL (1) NL171840C (en)
SE (1) SE437079B (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3071693D1 (en) * 1979-06-28 1986-09-18 Pasteur Institut Reagent for detecting parasitoses and allergies, detection process using this reagent and test kit therefor
US4321251A (en) * 1979-12-19 1982-03-23 The United States Of America As Represented By The Department Of Health And Human Services Detection of malignant lesions of the oral cavity utilizing toluidine blue rinse
US4555396A (en) * 1982-12-22 1985-11-26 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
SE461660B (en) * 1983-04-19 1990-03-12 Bio Instructa Labkonsult PROCEDURE FOR ANALYSIS OF RECEPTOR ACTIVITIES ON CELLS
HU186309B (en) * 1983-09-02 1985-07-29 Reanal Finomvegyszergyar Reagent for determining the thrombacyta and leucocyte number
EP0177352A1 (en) * 1984-10-04 1986-04-09 The State Of Victoria Improvements in or relating to enzyme-linked immunosorbent assay
JPS62135752U (en) * 1986-02-20 1987-08-26
JPH0448708Y2 (en) * 1986-04-10 1992-11-17
CA1309327C (en) * 1986-09-10 1992-10-27 Tomoyuki Kuroda Reagent and method for classifying leukocytes by flow cytometry
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
IL85532A (en) * 1987-03-13 1992-03-29 Coulter Electronics Method and lytic reagent system for isolation,identification and/or analysis of leukocytes from whole blood samples
US5155044A (en) * 1987-03-13 1992-10-13 Coulter Electronics, Inc. Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
PT106082A (en) * 2012-01-04 2013-07-04 Fernando Jorge Neves Ferreira FIXING COMPOSITION FOR CYTOLOGY, CELL FITTING METHOD AND ITS APPLICATIONS
US20240036066A1 (en) * 2020-12-22 2024-02-01 Radiometer Medical Aps Method for monitoring and adjusting reagent for hematology

Also Published As

Publication number Publication date
NL171840B (en) 1982-12-16
NL7607012A (en) 1976-12-29
CH613523A5 (en) 1979-09-28
JPS5224589A (en) 1977-02-24
DK289076A (en) 1976-12-28
NL171840C (en) 1983-05-16
DE2628468A1 (en) 1976-12-30
SE437079B (en) 1985-02-04
GB1560729A (en) 1980-02-06
DE2628468C2 (en) 1982-05-06
JPS5819063B2 (en) 1983-04-15
CA1087966A (en) 1980-10-21

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