GB1499846A - Method for analysis of endotoxin-precipitated limulus lysate - Google Patents

Method for analysis of endotoxin-precipitated limulus lysate

Info

Publication number
GB1499846A
GB1499846A GB1650975A GB1650975A GB1499846A GB 1499846 A GB1499846 A GB 1499846A GB 1650975 A GB1650975 A GB 1650975A GB 1650975 A GB1650975 A GB 1650975A GB 1499846 A GB1499846 A GB 1499846A
Authority
GB
United Kingdom
Prior art keywords
pyrogen
precipitated
limulus
protein
amount
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1650975A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter International Inc
Original Assignee
Baxter Travenol Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Travenol Laboratories Inc filed Critical Baxter Travenol Laboratories Inc
Publication of GB1499846A publication Critical patent/GB1499846A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1499846 Testing for pyrogens BAXTER TRAVENOL LABORATORIES Inc 22 April 1975 [1 May 1974] 16509/75 Heading G1B A material is tested for an unknown pyrogen by (A) calibrating a purified solution of portions of Limulus lysate, which portions precipitate limulus protein in the presence of a pyrogen by:- (i) Making serially diluted dispersions of pyrogen (a plurality containing 0À39 ng/ml or less) (ii) Adding a given amount of limulus lysate solution to each dispersion. (iii) Measuring, photometrically, the amount of limulus protein precipitated, an abrupt increase in the amount of said protein indicating pyrogen. (B) Adding to said material said given amount of fresh limulus lysate solution, under conditions similar to those for A (ii) and measuring photometrically the precipitated protein, an amount equal to or greater than that of the abrupt increase in A (iii) indicating the presence of pyrogen. The precipitated pyrogen may be removed from its supernate for quantitative analysis. In step A (ii) the reaction mixture may be incubated, e.g. at 37‹C for 60 minutes.
GB1650975A 1974-05-01 1975-04-22 Method for analysis of endotoxin-precipitated limulus lysate Expired GB1499846A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US46599074A 1974-05-01 1974-05-01

Publications (1)

Publication Number Publication Date
GB1499846A true GB1499846A (en) 1978-02-01

Family

ID=23849996

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1650975A Expired GB1499846A (en) 1974-05-01 1975-04-22 Method for analysis of endotoxin-precipitated limulus lysate

Country Status (6)

Country Link
JP (1) JPS50147989A (en)
AU (1) AU497873B2 (en)
CA (1) CA1040081A (en)
DE (1) DE2517860C3 (en)
FR (1) FR2280083A1 (en)
GB (1) GB1499846A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4221865A (en) 1978-10-06 1980-09-09 Baxter Travenol Laboratories, Inc. Method for determining endotoxin concentration
US4221866A (en) 1978-10-06 1980-09-09 Baxter Travenol Laboratories, Inc. Method for determining endotoxin concentration
US4376819A (en) 1979-09-14 1983-03-15 Baxter Travenol Laboratories, Inc. Biological extracts and method for making same

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3944391A (en) * 1974-12-27 1976-03-16 Preventive Systems, Inc. In vitro process for detecting endotoxin in a biological fluid
DE68916714T2 (en) * 1988-07-05 1995-01-12 Wako Pure Chem Ind Ltd Procedure for the determination of endotoxins.

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4221865A (en) 1978-10-06 1980-09-09 Baxter Travenol Laboratories, Inc. Method for determining endotoxin concentration
US4221866A (en) 1978-10-06 1980-09-09 Baxter Travenol Laboratories, Inc. Method for determining endotoxin concentration
US4376819A (en) 1979-09-14 1983-03-15 Baxter Travenol Laboratories, Inc. Biological extracts and method for making same

Also Published As

Publication number Publication date
DE2517860A1 (en) 1975-11-13
AU8069175A (en) 1976-11-04
JPS50147989A (en) 1975-11-27
DE2517860C3 (en) 1980-01-03
FR2280083B1 (en) 1982-02-05
CA1040081A (en) 1978-10-10
FR2280083A1 (en) 1976-02-20
AU497873B2 (en) 1979-01-18
DE2517860B2 (en) 1979-04-19

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Legal Events

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PCNP Patent ceased through non-payment of renewal fee