GB1499846A - Method for analysis of endotoxin-precipitated limulus lysate - Google Patents
Method for analysis of endotoxin-precipitated limulus lysateInfo
- Publication number
- GB1499846A GB1499846A GB1650975A GB1650975A GB1499846A GB 1499846 A GB1499846 A GB 1499846A GB 1650975 A GB1650975 A GB 1650975A GB 1650975 A GB1650975 A GB 1650975A GB 1499846 A GB1499846 A GB 1499846A
- Authority
- GB
- United Kingdom
- Prior art keywords
- pyrogen
- precipitated
- limulus
- protein
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1499846 Testing for pyrogens BAXTER TRAVENOL LABORATORIES Inc 22 April 1975 [1 May 1974] 16509/75 Heading G1B A material is tested for an unknown pyrogen by (A) calibrating a purified solution of portions of Limulus lysate, which portions precipitate limulus protein in the presence of a pyrogen by:- (i) Making serially diluted dispersions of pyrogen (a plurality containing 0À39 ng/ml or less) (ii) Adding a given amount of limulus lysate solution to each dispersion. (iii) Measuring, photometrically, the amount of limulus protein precipitated, an abrupt increase in the amount of said protein indicating pyrogen. (B) Adding to said material said given amount of fresh limulus lysate solution, under conditions similar to those for A (ii) and measuring photometrically the precipitated protein, an amount equal to or greater than that of the abrupt increase in A (iii) indicating the presence of pyrogen. The precipitated pyrogen may be removed from its supernate for quantitative analysis. In step A (ii) the reaction mixture may be incubated, e.g. at 37‹C for 60 minutes.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US46599074A | 1974-05-01 | 1974-05-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1499846A true GB1499846A (en) | 1978-02-01 |
Family
ID=23849996
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1650975A Expired GB1499846A (en) | 1974-05-01 | 1975-04-22 | Method for analysis of endotoxin-precipitated limulus lysate |
Country Status (6)
Country | Link |
---|---|
JP (1) | JPS50147989A (en) |
AU (1) | AU497873B2 (en) |
CA (1) | CA1040081A (en) |
DE (1) | DE2517860C3 (en) |
FR (1) | FR2280083A1 (en) |
GB (1) | GB1499846A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4221866A (en) | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
US4221865A (en) | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
US4376819A (en) | 1979-09-14 | 1983-03-15 | Baxter Travenol Laboratories, Inc. | Biological extracts and method for making same |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3944391A (en) * | 1974-12-27 | 1976-03-16 | Preventive Systems, Inc. | In vitro process for detecting endotoxin in a biological fluid |
DE68916714T2 (en) * | 1988-07-05 | 1995-01-12 | Wako Pure Chem Ind Ltd | Procedure for the determination of endotoxins. |
-
1975
- 1975-04-11 CA CA224,426A patent/CA1040081A/en not_active Expired
- 1975-04-22 GB GB1650975A patent/GB1499846A/en not_active Expired
- 1975-04-22 DE DE19752517860 patent/DE2517860C3/en not_active Expired
- 1975-04-28 JP JP5124975A patent/JPS50147989A/ja active Pending
- 1975-04-28 FR FR7513235A patent/FR2280083A1/en active Granted
- 1975-04-30 AU AU80691/75A patent/AU497873B2/en not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4221866A (en) | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
US4221865A (en) | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
US4376819A (en) | 1979-09-14 | 1983-03-15 | Baxter Travenol Laboratories, Inc. | Biological extracts and method for making same |
Also Published As
Publication number | Publication date |
---|---|
FR2280083B1 (en) | 1982-02-05 |
DE2517860C3 (en) | 1980-01-03 |
CA1040081A (en) | 1978-10-10 |
AU8069175A (en) | 1976-11-04 |
AU497873B2 (en) | 1979-01-18 |
FR2280083A1 (en) | 1976-02-20 |
DE2517860B2 (en) | 1979-04-19 |
DE2517860A1 (en) | 1975-11-13 |
JPS50147989A (en) | 1975-11-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |