FR2315251A1 - Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276) - Google Patents

Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Info

Publication number
FR2315251A1
FR2315251A1 FR7520273A FR7520273A FR2315251A1 FR 2315251 A1 FR2315251 A1 FR 2315251A1 FR 7520273 A FR7520273 A FR 7520273A FR 7520273 A FR7520273 A FR 7520273A FR 2315251 A1 FR2315251 A1 FR 2315251A1
Authority
FR
France
Prior art keywords
basophils
counting
acid
reagent
metachromatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7520273A
Other languages
French (fr)
Other versions
FR2315251B1 (en
Inventor
Jacques Benveniste
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Priority to FR7520273A priority Critical patent/FR2315251A1/en
Priority to CH787076A priority patent/CH613523A5/en
Priority to SE7607282A priority patent/SE437079B/en
Priority to NL7607012A priority patent/NL171840C/en
Priority to CA255,681A priority patent/CA1087966A/en
Priority to DK289076A priority patent/DK289076A/en
Priority to DE19762628468 priority patent/DE2628468C2/en
Priority to BE168354A priority patent/BE843452A/en
Priority to JP51076303A priority patent/JPS5819063B2/en
Priority to IT2481876A priority patent/IT1063845B/en
Priority to GB2676876A priority patent/GB1560729A/en
Publication of FR2315251A1 publication Critical patent/FR2315251A1/en
Application granted granted Critical
Publication of FR2315251B1 publication Critical patent/FR2315251B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Ecology (AREA)
  • Physiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Novel reagent for counting basophils and opt. other leukocytes in a biological medium (esp. a blood sample) consists of a hypotonic aq. soln. contg. a metachromatic reagent (pref. toluidine blue), a fixative for leukocytes and basophils, and an acid capable of contributing to the disruption of erythrocytes in blood samples. The acid is present in such an amt. that the final pH of the reagent is 3-5. The process comprises directly mixing the sample, pref. in a single step, with the above reagent. Visualisation and counting of basophils is used in a variety of diagnostic procedures, pref. for the diagnosis of anaphylactic sensitivities, esp. allergies, involving incubating several fractions of a blood sample with 1 agent (esp. an allergen) having a degranulating effect on basophils.
FR7520273A 1975-06-27 1975-06-27 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276) Granted FR2315251A1 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
FR7520273A FR2315251A1 (en) 1975-06-27 1975-06-27 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)
CH787076A CH613523A5 (en) 1975-06-27 1976-06-21 Method for displaying basophils
SE7607282A SE437079B (en) 1975-06-27 1976-06-24 SET TO VISUALIZE BASOFILES, REAGENTS FOR VISUALIZING BASOFILES, AND USE OF THE SET AND REAGENTS
CA255,681A CA1087966A (en) 1975-06-27 1976-06-25 Process and a metachromatic composition for effecting a count of leucocytes, and more particularly of basophiles
DK289076A DK289076A (en) 1975-06-27 1976-06-25 PROCEDURE AND COLOR CHANGE FOR LISTING LEUCOCYTS ISER BASOPHILE BLOOD BODIES
DE19762628468 DE2628468C2 (en) 1975-06-27 1976-06-25 Method for staining basophils and reagent for carrying out this method
NL7607012A NL171840C (en) 1975-06-27 1976-06-25 METHOD FOR PREPARING A REAGENT FOR COUNTING BASOFIELS.
BE168354A BE843452A (en) 1975-06-27 1976-06-25 METHOD AND METACHROMATIC COMPOSITION FOR NUMBERING LEUCOCYTES, MORE PARTICULARLY BASOPHILS
JP51076303A JPS5819063B2 (en) 1975-06-27 1976-06-28 Reagent for counting leukocytes, especially basophil cells, and calculation method using the reagent
IT2481876A IT1063845B (en) 1975-06-27 1976-06-28 PROCEDURE FOR CALCULATING THE NUMBER OF LEUKOCYTES AND IN PARTICULAR BLOOD GASOPHILES NOT REACTIVE TO ALLOW THIS CALCULATION AND CONSEQUENTLY THE DIAGNOSIS INVITATION OF THE CAUSES OF ALLERGIC AND SIMILAR AFFECTIONS
GB2676876A GB1560729A (en) 1975-06-27 1976-06-28 Counting of leucocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7520273A FR2315251A1 (en) 1975-06-27 1975-06-27 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Publications (2)

Publication Number Publication Date
FR2315251A1 true FR2315251A1 (en) 1977-01-21
FR2315251B1 FR2315251B1 (en) 1981-06-19

Family

ID=9157192

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7520273A Granted FR2315251A1 (en) 1975-06-27 1975-06-27 Basophils visualisation and counting - using hypotonic reagent solutions contg. a metachromatic agent, a fixative, and an acid (BE271276)

Country Status (3)

Country Link
BE (1) BE843452A (en)
FR (1) FR2315251A1 (en)
IT (1) IT1063845B (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0022698A1 (en) * 1979-06-28 1981-01-21 Institut Pasteur Reagent for detecting parasitoses and allergies, detection process using this reagent and test kit therefor
FR2467401A1 (en) * 1979-10-12 1981-04-17 Weck Alain De Measuring de-granulation of basophiles by flow cytometry - after staining leucocytes with Alcian blue, useful in diagnosing allergies
FR2478317A1 (en) * 1980-03-12 1981-09-18 Kass Lawrence METHOD AND COMPOSITION FOR DETERMINING INDIVIDUAL CATEGORIES OF LEUKOCYTES BY DIFFERENTIAL SORPTION OF AT LEAST ONE METACHROMATIC DYE
FR2501865A2 (en) * 1981-03-11 1982-09-17 Kass Lawrence METHOD FOR THE ANALYSIS, BY DIFFERENTIAL SORPTION OF A METACHROMATIC COLOR, OF VARIOUS BLOOD LEUKOCYTES
FR2530028A2 (en) * 1982-07-09 1984-01-13 Pasteur Institut NOVEL REAGENT FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES, PROCESS FOR THE PREPARATION OF THIS NEW REAGENT AND METHOD FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES USING SAID REAGENT

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0022698A1 (en) * 1979-06-28 1981-01-21 Institut Pasteur Reagent for detecting parasitoses and allergies, detection process using this reagent and test kit therefor
US4640897A (en) * 1979-06-28 1987-02-03 Institut Pasteur Immunoanalysis of basophil-containing blood fraction for diagnosing parasitoses and allergies
FR2467401A1 (en) * 1979-10-12 1981-04-17 Weck Alain De Measuring de-granulation of basophiles by flow cytometry - after staining leucocytes with Alcian blue, useful in diagnosing allergies
FR2478317A1 (en) * 1980-03-12 1981-09-18 Kass Lawrence METHOD AND COMPOSITION FOR DETERMINING INDIVIDUAL CATEGORIES OF LEUKOCYTES BY DIFFERENTIAL SORPTION OF AT LEAST ONE METACHROMATIC DYE
FR2501865A2 (en) * 1981-03-11 1982-09-17 Kass Lawrence METHOD FOR THE ANALYSIS, BY DIFFERENTIAL SORPTION OF A METACHROMATIC COLOR, OF VARIOUS BLOOD LEUKOCYTES
FR2530028A2 (en) * 1982-07-09 1984-01-13 Pasteur Institut NOVEL REAGENT FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES, PROCESS FOR THE PREPARATION OF THIS NEW REAGENT AND METHOD FOR THE DIAGNOSIS OF PARASITOSES AND ALLERGIES USING SAID REAGENT
EP0099812A1 (en) * 1982-07-09 1984-02-01 Institut Pasteur Reagent for the diagnosis of parasitosis and allergies, method for the preparation of this reagent and method of diagnosing parasitosis and allergies using this reagent
WO1984000420A1 (en) * 1982-07-09 1984-02-02 Pasteur Institut New reagent for the diagnostic of parasitosis and allergia, process for the preparation of this new reagent and process for the diagnostic of parasitosis and allergia by means of said reagent

Also Published As

Publication number Publication date
BE843452A (en) 1976-12-27
IT1063845B (en) 1985-02-18
FR2315251B1 (en) 1981-06-19

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