KR960706558A - 골 발육 촉진성 단백질의 돌연변이체(mutants of bone morphogenetic proteins) - Google Patents
골 발육 촉진성 단백질의 돌연변이체(mutants of bone morphogenetic proteins)Info
- Publication number
- KR960706558A KR960706558A KR1019960703011A KR19960703011A KR960706558A KR 960706558 A KR960706558 A KR 960706558A KR 1019960703011 A KR1019960703011 A KR 1019960703011A KR 19960703011 A KR19960703011 A KR 19960703011A KR 960706558 A KR960706558 A KR 960706558A
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- Prior art keywords
- bmp
- amino acid
- mutant
- seq
- substituted
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
골 발육 촉진성 단백질(BMP)의 돌연변이 형태를 코오딩하는 DNA 분자가 밝혀졌다. -BMP의 돌연변이 형태는 박테리아에 의해 생성될 수 있으며, 생물학적으로 활성이 있는 BMP의 단일이합체 또는 이형이합체를 생성하기 위해 재폴딩 될 수 있다. 또한, 이러한 돌연변이체 BMP를 제조하는 방법이 밝혀졌다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 BMP-2, 4, 5, 6, 7 및 8의 서열을 비교한 도면이다.
Claims (17)
- 319 내지 321의 3 누클레오티드가 아스파르트산을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 3에 나타난 바와 같은 BMP-4를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
- 166 내지 168의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 5에 나타난 바와 같은 BMP-5를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
- 166 내지 168의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 7에 나타난 바와 같은 BMP-6를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
- 187 내지 189의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 9에 나타난 바와 같은 BMP-7를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
- 187 내지 189의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 11에 나타난 바와 같은 BMP-8를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
- 아미노산 107 잔기인 글루탐산이 아스파트산으로 치환된 서열 ID 번호 : 4의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-4의 정제 조성물.
- 아미노산 56 잔기인 알라닌이 히스티딘으로 치환된 서열 ID 번호 : 6의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-5의 정제 조성물.
- 아미노산 56 잔기인 알라닌이 히스티딘으로 치환된 서열 ID 번호 : 8의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-6의 정제 조성물.
- 아미노산 63 잔기인 알라닌이 히스티딘으로 치환된 서열 ID 번호 : 10의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-7의 정제 조성물.
- 아미노산 63 잔기인 세린이 히스티딘으로 치환된 서열 ID 번호 : 12의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-8의 정제 조성물.
- 제6항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
- 제7항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
- 제8항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
- 제9항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
- 제10항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
- 증진된 재폴딩 특성을 갖는 골 발육 촉진성 단백질(BMP)의 돌연변이체를 수득하는 방법으로 : a) 재폴딩이 잘되는 것으로 밝혀진 BMP의 아미노산 서열(BMP+)과 재폴딩이 잘되지 않는 BMP의 아미노산 서열(BMP-)의 비교; b) (a) 단계에서의 비교를 통해 상호 관계가 있는 아미노산 위치에서의 차이를 결정; c) 변형 BMP- 단백질을 형성하기 위해, BMP+의 상호 관계가 있는 아미노산과 차이가 있는 아미노산이 BMP+의 상호 관계가 있는 아미노산으로 치환되도록 BMP-의 아미노산 서열 변경; d) 재폴딩 능력에 대한 BMP- 단백질의 테스트로 이루어지는 것을 특징으로 하는 방법.
- 제16항의 방법으로 생성된 증진된 재폴딩 특성을 갖는 골 발육 촉진성 단백질(BMP)의 돌연변이체.※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/163877 | 1993-12-07 | ||
US08/163,877 | 1993-12-07 | ||
US08/163,877 US5399677A (en) | 1993-12-07 | 1993-12-07 | Mutants of bone morphogenetic proteins |
Publications (2)
Publication Number | Publication Date |
---|---|
KR960706558A true KR960706558A (ko) | 1996-12-09 |
KR100361058B1 KR100361058B1 (ko) | 2003-04-10 |
Family
ID=22591964
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019960703011A KR100361058B1 (ko) | 1993-12-07 | 1994-11-15 | 골발육촉진성단백질의돌연변이체 |
Country Status (16)
Country | Link |
---|---|
US (3) | US5399677A (ko) |
EP (1) | EP0733108B1 (ko) |
JP (1) | JP3704350B2 (ko) |
KR (1) | KR100361058B1 (ko) |
AT (1) | ATE200515T1 (ko) |
AU (1) | AU682238B2 (ko) |
CA (1) | CA2176943C (ko) |
DE (1) | DE69427093T2 (ko) |
DK (1) | DK0733108T3 (ko) |
ES (1) | ES2157313T3 (ko) |
FI (1) | FI118428B (ko) |
GR (1) | GR3035769T3 (ko) |
NO (1) | NO317800B1 (ko) |
OA (1) | OA10294A (ko) |
PT (1) | PT733108E (ko) |
WO (1) | WO1995016034A1 (ko) |
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KR20190005239A (ko) * | 2016-05-31 | 2019-01-15 | 재단법인 목암생명과학연구소 | Tgf-베타 슈퍼패밀리의 ab6 패밀리 디자이너 리간드를 포함하는 간질환 치료용 조성물 및 그의 이용 |
CN115335393A (zh) * | 2020-03-24 | 2022-11-11 | 博奥泰克尼公司 | 使用TGFβ敲除细胞系及由其产生的组合物的方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
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GR79124B (ko) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
ZA874681B (en) * | 1986-07-01 | 1988-04-27 | Genetics Inst | Novel osteoinductive factors |
US5106748A (en) * | 1986-07-01 | 1992-04-21 | Genetics Institute, Inc. | Dna sequences encoding 5 proteins |
US5013649A (en) * | 1986-07-01 | 1991-05-07 | Genetics Institute, Inc. | DNA sequences encoding osteoinductive products |
US5187076A (en) * | 1986-07-01 | 1993-02-16 | Genetics Institute, Inc. | DNA sequences encoding BMP-6 proteins |
GB8927546D0 (en) * | 1989-12-06 | 1990-02-07 | Ciba Geigy | Process for the production of biologically active tgf-beta |
ES2168091T3 (es) * | 1990-05-16 | 2002-06-01 | Genetics Inst | Proteinas de induccion osea y cartilaginosa. |
AU660019B2 (en) * | 1991-03-11 | 1995-06-08 | Stryker Corporation | Protein-induced morphogenesis |
ATE175441T1 (de) * | 1991-06-25 | 1999-01-15 | Genetics Inst | Bmp-9 zusammensetzungen |
KR100259827B1 (ko) * | 1991-11-04 | 2000-06-15 | 브루스 엠. 에이센, 토마스 제이 데스로저 | 재조합 골형태 형성 단백질 헤테로다이머, 그 조성물 및 사용방법 |
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1993
- 1993-12-07 US US08/163,877 patent/US5399677A/en not_active Expired - Lifetime
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1994
- 1994-11-15 DE DE69427093T patent/DE69427093T2/de not_active Expired - Lifetime
- 1994-11-15 ES ES95903116T patent/ES2157313T3/es not_active Expired - Lifetime
- 1994-11-15 CA CA002176943A patent/CA2176943C/en not_active Expired - Lifetime
- 1994-11-15 PT PT95903116T patent/PT733108E/pt unknown
- 1994-11-15 WO PCT/US1994/013181 patent/WO1995016034A1/en active IP Right Grant
- 1994-11-15 AU AU12093/95A patent/AU682238B2/en not_active Ceased
- 1994-11-15 KR KR1019960703011A patent/KR100361058B1/ko not_active IP Right Cessation
- 1994-11-15 DK DK95903116T patent/DK0733108T3/da active
- 1994-11-15 JP JP51619695A patent/JP3704350B2/ja not_active Expired - Fee Related
- 1994-11-15 AT AT95903116T patent/ATE200515T1/de not_active IP Right Cessation
- 1994-11-15 EP EP95903116A patent/EP0733108B1/en not_active Expired - Lifetime
- 1994-12-21 US US08/360,914 patent/US5756308A/en not_active Expired - Lifetime
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1996
- 1996-06-04 NO NO19962303A patent/NO317800B1/no unknown
- 1996-06-06 FI FI962349A patent/FI118428B/fi active IP Right Grant
- 1996-06-07 OA OA60839A patent/OA10294A/en unknown
- 1996-10-31 US US08/741,589 patent/US5804416A/en not_active Expired - Lifetime
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Also Published As
Publication number | Publication date |
---|---|
KR100361058B1 (ko) | 2003-04-10 |
US5756308A (en) | 1998-05-26 |
NO317800B1 (no) | 2004-12-13 |
AU682238B2 (en) | 1997-09-25 |
PT733108E (pt) | 2001-08-30 |
JPH09506259A (ja) | 1997-06-24 |
WO1995016034A1 (en) | 1995-06-15 |
JP3704350B2 (ja) | 2005-10-12 |
DK0733108T3 (da) | 2001-06-18 |
US5804416A (en) | 1998-09-08 |
CA2176943A1 (en) | 1995-06-15 |
FI962349A (fi) | 1996-07-16 |
FI962349A0 (fi) | 1996-06-06 |
EP0733108A1 (en) | 1996-09-25 |
DE69427093T2 (de) | 2001-11-15 |
GR3035769T3 (en) | 2001-07-31 |
DE69427093D1 (de) | 2001-05-17 |
OA10294A (en) | 1997-10-07 |
EP0733108B1 (en) | 2001-04-11 |
FI118428B (fi) | 2007-11-15 |
NO962303L (no) | 1996-06-04 |
AU1209395A (en) | 1995-06-27 |
CA2176943C (en) | 2007-04-03 |
US5399677A (en) | 1995-03-21 |
ATE200515T1 (de) | 2001-04-15 |
NO962303D0 (no) | 1996-06-04 |
ES2157313T3 (es) | 2001-08-16 |
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