KR960706558A - 골 발육 촉진성 단백질의 돌연변이체(mutants of bone morphogenetic proteins) - Google Patents

골 발육 촉진성 단백질의 돌연변이체(mutants of bone morphogenetic proteins)

Info

Publication number
KR960706558A
KR960706558A KR1019960703011A KR19960703011A KR960706558A KR 960706558 A KR960706558 A KR 960706558A KR 1019960703011 A KR1019960703011 A KR 1019960703011A KR 19960703011 A KR19960703011 A KR 19960703011A KR 960706558 A KR960706558 A KR 960706558A
Authority
KR
South Korea
Prior art keywords
bmp
amino acid
mutant
seq
substituted
Prior art date
Application number
KR1019960703011A
Other languages
English (en)
Other versions
KR100361058B1 (ko
Inventor
엠. 울프만 네일
멕코이 존
Original Assignee
데스로이져 토마스 제이.
제네틱스 인스티튜트, 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 데스로이져 토마스 제이., 제네틱스 인스티튜트, 인코포레이티드 filed Critical 데스로이져 토마스 제이.
Publication of KR960706558A publication Critical patent/KR960706558A/ko
Application granted granted Critical
Publication of KR100361058B1 publication Critical patent/KR100361058B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Rheumatology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

골 발육 촉진성 단백질(BMP)의 돌연변이 형태를 코오딩하는 DNA 분자가 밝혀졌다. -BMP의 돌연변이 형태는 박테리아에 의해 생성될 수 있으며, 생물학적으로 활성이 있는 BMP의 단일이합체 또는 이형이합체를 생성하기 위해 재폴딩 될 수 있다. 또한, 이러한 돌연변이체 BMP를 제조하는 방법이 밝혀졌다.

Description

골 발육 촉진성 단백질의 돌연변이체(MUTANTS OF BONE MORPHOGENETIC PROTEINS)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 BMP-2, 4, 5, 6, 7 및 8의 서열을 비교한 도면이다.

Claims (17)

  1. 319 내지 321의 3 누클레오티드가 아스파르트산을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 3에 나타난 바와 같은 BMP-4를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
  2. 166 내지 168의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 5에 나타난 바와 같은 BMP-5를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
  3. 166 내지 168의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 7에 나타난 바와 같은 BMP-6를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
  4. 187 내지 189의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 9에 나타난 바와 같은 BMP-7를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
  5. 187 내지 189의 3 누클레오티드가 히스티딘을 코오딩하는 3 누클레오티드로 치환된 것을 제외하고는, 서열 ID 번호 : 11에 나타난 바와 같은 BMP-8를 코오딩하는 누클레오티드 서열로 이루어지는 것을 특징으로 하는 재조합 DNA 분자.
  6. 아미노산 107 잔기인 글루탐산이 아스파트산으로 치환된 서열 ID 번호 : 4의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-4의 정제 조성물.
  7. 아미노산 56 잔기인 알라닌이 히스티딘으로 치환된 서열 ID 번호 : 6의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-5의 정제 조성물.
  8. 아미노산 56 잔기인 알라닌이 히스티딘으로 치환된 서열 ID 번호 : 8의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-6의 정제 조성물.
  9. 아미노산 63 잔기인 알라닌이 히스티딘으로 치환된 서열 ID 번호 : 10의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-7의 정제 조성물.
  10. 아미노산 63 잔기인 세린이 히스티딘으로 치환된 서열 ID 번호 : 12의 아미노산 서열로 이루어지는 것을 특징으로 하는 돌연변이체 BMP-8의 정제 조성물.
  11. 제6항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
  12. 제7항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
  13. 제8항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
  14. 제9항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
  15. 제10항에 따른 하나 이상의 돌연변이체 BMP로 이루어지는 것을 특징으로 하는 BMP 이형 이합체의 정제 조성물.
  16. 증진된 재폴딩 특성을 갖는 골 발육 촉진성 단백질(BMP)의 돌연변이체를 수득하는 방법으로 : a) 재폴딩이 잘되는 것으로 밝혀진 BMP의 아미노산 서열(BMP＋)과 재폴딩이 잘되지 않는 BMP의 아미노산 서열(BMP-)의 비교; b) (a) 단계에서의 비교를 통해 상호 관계가 있는 아미노산 위치에서의 차이를 결정; c) 변형 BMP- 단백질을 형성하기 위해, BMP＋의 상호 관계가 있는 아미노산과 차이가 있는 아미노산이 BMP＋의 상호 관계가 있는 아미노산으로 치환되도록 BMP-의 아미노산 서열 변경; d) 재폴딩 능력에 대한 BMP- 단백질의 테스트로 이루어지는 것을 특징으로 하는 방법.
  17. 제16항의 방법으로 생성된 증진된 재폴딩 특성을 갖는 골 발육 촉진성 단백질(BMP)의 돌연변이체.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019960703011A 1993-12-07 1994-11-15 골발육촉진성단백질의돌연변이체 KR100361058B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/163877 1993-12-07
US08/163,877 1993-12-07
US08/163,877 US5399677A (en) 1993-12-07 1993-12-07 Mutants of bone morphogenetic proteins

Publications (2)

Publication Number Publication Date
KR960706558A true KR960706558A (ko) 1996-12-09
KR100361058B1 KR100361058B1 (ko) 2003-04-10

Family

ID=22591964

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019960703011A KR100361058B1 (ko) 1993-12-07 1994-11-15 골발육촉진성단백질의돌연변이체

Country Status (16)

Country Link
US (3) US5399677A (ko)
EP (1) EP0733108B1 (ko)
JP (1) JP3704350B2 (ko)
KR (1) KR100361058B1 (ko)
AT (1) ATE200515T1 (ko)
AU (1) AU682238B2 (ko)
CA (1) CA2176943C (ko)
DE (1) DE69427093T2 (ko)
DK (1) DK0733108T3 (ko)
ES (1) ES2157313T3 (ko)
FI (1) FI118428B (ko)
GR (1) GR3035769T3 (ko)
NO (1) NO317800B1 (ko)
OA (1) OA10294A (ko)
PT (1) PT733108E (ko)
WO (1) WO1995016034A1 (ko)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150328A (en) * 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
US20080139474A1 (en) * 1991-11-04 2008-06-12 David Israel Recombinant bone morphogenetic protein heterodimers, compositions and methods of use
KR100259827B1 (ko) * 1991-11-04 2000-06-15 브루스 엠. 에이센, 토마스 제이 데스로저 재조합 골형태 형성 단백질 헤테로다이머, 그 조성물 및 사용방법
US6291206B1 (en) * 1993-09-17 2001-09-18 Genetics Institute, Inc. BMP receptor proteins
EP0733109B9 (en) * 1993-12-07 2006-07-05 Genetics Institute, LLC Bmp-12, bmp-13 and tendon-inducing compositions thereof
US5942496A (en) 1994-02-18 1999-08-24 The Regent Of The University Of Michigan Methods and compositions for multiple gene transfer into bone cells
US6551618B2 (en) * 1994-03-15 2003-04-22 University Of Birmingham Compositions and methods for delivery of agents for neuronal regeneration and survival
PL186518B1 (pl) 1995-04-19 2004-01-30 Bioph Biotech Entw Pharm Gmbh Białko, kompozycja farmaceutyczna i sposób wytwarzania białka
US6040431A (en) 1995-06-07 2000-03-21 Stryker Corporation Single chain analogs of the TGF-β superfamily (morphons)
US6048964A (en) * 1995-12-12 2000-04-11 Stryker Corporation Compositions and therapeutic methods using morphogenic proteins and stimulatory factors
US7026292B1 (en) 1995-12-12 2006-04-11 Stryker Corporation Compositions and therapeutic methods using morphogenic proteins and stimulatory factors
US5869041A (en) * 1996-01-12 1999-02-09 The Miriam Hospital Delivery of bioactive compounds to an organism
US7811793B2 (en) * 1996-12-25 2010-10-12 Biopharma Gesellschaft Zur Biotechnologischen Entwicklung Von Pharmaka Mbh Process for preparing purified active monomer of bone-derived factor
US20040019185A1 (en) * 1996-12-25 2004-01-29 Hidetoshi Andou Process for preparing purified active monomer of bone-derived factor
ZA9711580B (en) * 1996-12-25 1999-09-23 Hoechst Marion Roussel Ltd Process for the production of purified dimeric bone morphogenetic factors.
US20020052026A1 (en) * 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6391311B1 (en) 1998-03-17 2002-05-21 Genentech, Inc. Polypeptides having homology to vascular endothelial cell growth factor and bone morphogenetic protein 1
NZ525914A (en) 1998-03-10 2004-03-26 Genentech Inc Novel polypeptides and nucleic acids encoding the same
CA2322792C (en) 1998-03-17 2010-09-14 Genentech, Inc. Polypeptides homologous to vegf and bmp1
US6677432B1 (en) 1998-10-07 2004-01-13 Stryker Corporation Mutations of the C-terminal portion of TGF-β superfamily proteins
CA2657302A1 (en) * 1998-10-07 2000-04-13 Hermann Oppermann Modified tgf-.beta. superfamily proteins
US6846906B1 (en) 1998-10-07 2005-01-25 Stryker Corporation Modified proteins of the TGF-β superfamily, including morphogenic proteins
US6727224B1 (en) * 1999-02-01 2004-04-27 Genetics Institute, Llc. Methods and compositions for healing and repair of articular cartilage
DE19906096A1 (de) 1999-02-13 2000-08-17 Walter Sebald Protein mit einem Heparin-bindenden Epitop
DE19944626A1 (de) * 1999-09-17 2001-03-22 Knoell Hans Forschung Ev Verfahren zur Herstellung von dimeren, biologisch aktiven knochenmorphogenetischen Proteinen
ATE271886T1 (de) 1999-10-15 2004-08-15 Inst Genetics Llc Hyaluronsäure enthaltende zusammensetzungen zur abgabe osteogener proteine
US7740841B1 (en) 2000-01-28 2010-06-22 Sunnybrook Health Science Center Therapeutic method for reducing angiogenesis
US20030104977A1 (en) * 2000-03-31 2003-06-05 Ugo Ripamonti Methods for inducing angiogenesis using morphogenic proteins and stimulatory factors
DE10026713A1 (de) * 2000-05-30 2001-12-06 Walter Sebald Mutein einer Kette eines Proteins aus der Superfamilie des Wachstumsfaktors TGF-beta
US20030082233A1 (en) * 2000-12-01 2003-05-01 Lyons Karen M. Method and composition for modulating bone growth
ES2305246T3 (es) * 2001-06-01 2008-11-01 Wyeth Composiciones para la administracion sistemica de secuencias que codifican proteinas oseas morfogeneticas.
TWI267378B (en) * 2001-06-08 2006-12-01 Wyeth Corp Calcium phosphate delivery vehicles for osteoinductive proteins
FI117667B (fi) * 2001-07-05 2007-01-15 Univ Zuerich Farmaseuttinen koostumus, joka soveltuu käytettäväksi ortopediassa ja hammaslääketieteessä
US20050287135A1 (en) * 2002-05-17 2005-12-29 Wyeth Injectable solid hyaluronic acid carriers for delivery of osteogenic proteins
US20040023322A1 (en) * 2002-08-01 2004-02-05 Goodheart Clyde R. Method of producing non-recombinant BMP-2 and use thereof
WO2005005459A1 (ja) * 2003-07-09 2005-01-20 National Institute Of Advanced Industrial Science And Technology タンパク質巻き戻し材料
EP1772154A3 (en) * 2003-09-12 2011-09-07 Wyeth LLC Injectable calcium phosphate pastes for delivery of osteogenic proteins
EP2441474B1 (en) 2003-10-17 2015-08-05 Joslin Diabetes Center, Inc. Methods and compositions for modulating adipocyte function
US20070066525A1 (en) * 2004-02-04 2007-03-22 Lee John C Compositions and therapeutic methods using morphogenic proteins
EP1753458A4 (en) * 2004-05-19 2009-07-22 Wyeth Corp MODULATION OF IMMUNOGLOBULIN PRODUCTION AND ATOPIC DISORDERS
EP1773394A2 (en) * 2004-08-05 2007-04-18 Wyeth a Corporation of the State of Delaware Antagonizing interleukin-21 receptor activity
PE20061391A1 (es) * 2005-02-14 2007-01-09 Wyeth Corp Anticuerpos para interleucina-17f y otros antagonistas de la senalizacion de il-17f
BRPI0608210A2 (pt) * 2005-02-14 2010-11-09 Wyeth Corp métodos de diagnóstico de um distúrbio relacionado com il-17f em um indivìduo e de seleção de compostos capazes de inibirem a ligação de il-17f em il-17r, uso de um antagonista de il-17f, composição farmacêutica, e, adjuvante de vacina
CN101309698A (zh) * 2005-03-30 2008-11-19 惠氏公司 通过施用bmp来刺激毛发生长的方法
GT200600148A (es) 2005-04-14 2006-11-22 Metodos para el tratamiento y la prevencion de fibrosis
EP1885750B1 (en) 2005-05-27 2011-08-17 BBS - Bioactive Bone Substitutes Oy Bone morphogenetic protein 4 and osteogenic devices and pharmaceutical products containing thereof
EP1885749B1 (en) 2005-05-27 2017-08-23 BBS-Bioactive Bone Substitutes Oy Bone morphogenetic protein 3 and osteogenic devices and pharmaceutical products containing thereof
WO2006125868A1 (en) 2005-05-27 2006-11-30 Bbs-Bioactive Bone Substitutes Oy Bone morphogenetic proteins containing a heparin binding site and osteogenic devices and pharmaceutical products containing thereof
US20100150885A1 (en) 2005-06-01 2010-06-17 Joslin Diabetes Center, Inc. Methods and compositions for inducing brown adipogenesis
GB0604966D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Medicaments and proteins
GB0604938D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Proteins, nucleic acids and medicaments
GB0604964D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Protein folding
EP2024495A2 (en) * 2006-05-25 2009-02-18 Wyeth Expression of the cysteine protease legumain in vascular and inflammatory diseases
CA2668771C (en) * 2006-11-08 2016-03-15 Wyeth Rationally designed media for cell culture
ES2447516T3 (es) 2006-12-21 2014-03-12 Stryker Corporation Formulaciones de liberación sostenida que comprenden cristales BMP-7
TW200902064A (en) * 2007-03-28 2009-01-16 Wyeth Corp Methods and compositions for modulating IL-17F/IL-17A biological activity
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
EP2019117A1 (en) * 2007-07-27 2009-01-28 BIOPHARM GESELLSCHAFT ZUR BIOTECHNOLOGISCHEN ENTWICKLUNG VON PHARMAKA mbH Optimized purification process of recombinant growth factor protein
EP2188371B1 (en) * 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
US8058407B2 (en) * 2008-10-31 2011-11-15 Wyeth Llc Purification of acidic proteins using ceramic hydroxyapatite chromatography
WO2010093925A2 (en) 2009-02-12 2010-08-19 Stryker Corporation PERIPHERAL ADMINISTRATION OF PROTEINS INCLUDING TGF-β SUPERFAMILY MEMBERS FOR TREATMENT OF SYSTEMIC DISORDERS AND DISEASE
AU2010213591B2 (en) 2009-02-12 2013-11-21 Stryker Corporation Compositions and methods for minimally-invasive systemic delivery of proteins including TGF-beta superfamily members
US20120148539A1 (en) 2009-03-24 2012-06-14 Moulay Hicham Alaoui-Ismaili Methods and Compositions for Tissue Engineering
US20110224138A1 (en) 2009-09-09 2011-09-15 Julie Krop Methods for treating pain induced by injuries and diseases of an articular joint
US20110224410A1 (en) 2009-09-17 2011-09-15 Hile David Buffers for Controlling the pH of Bone Morphogenetic Proteins
AU2010341565A1 (en) 2009-12-22 2012-07-12 Stryker Corporation BMP-7 variants with reduced immunogenicity
WO2017209523A1 (en) * 2016-05-31 2017-12-07 Mogam Institute For Biomedical Research Composition comprising an ab6 family designer ligand of tgf-beta superfamily for treating bone and cartilage disease and use thereof
EP3464337A4 (en) 2016-05-31 2019-12-18 Mogam Institute for Biomedical Research AB6 FAMILY DESIGNER LIGANDS OF THE TGF-BETA SUPERFAMILY
KR20190005239A (ko) * 2016-05-31 2019-01-15 재단법인 목암생명과학연구소 Tgf-베타 슈퍼패밀리의 ab6 패밀리 디자이너 리간드를 포함하는 간질환 치료용 조성물 및 그의 이용
CN115335393A (zh) * 2020-03-24 2022-11-11 博奥泰克尼公司 使用TGFβ敲除细胞系及由其产生的组合物的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR79124B (ko) * 1982-12-22 1984-10-02 Genentech Inc
ZA874681B (en) * 1986-07-01 1988-04-27 Genetics Inst Novel osteoinductive factors
US5106748A (en) * 1986-07-01 1992-04-21 Genetics Institute, Inc. Dna sequences encoding 5 proteins
US5013649A (en) * 1986-07-01 1991-05-07 Genetics Institute, Inc. DNA sequences encoding osteoinductive products
US5187076A (en) * 1986-07-01 1993-02-16 Genetics Institute, Inc. DNA sequences encoding BMP-6 proteins
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
ES2168091T3 (es) * 1990-05-16 2002-06-01 Genetics Inst Proteinas de induccion osea y cartilaginosa.
AU660019B2 (en) * 1991-03-11 1995-06-08 Stryker Corporation Protein-induced morphogenesis
ATE175441T1 (de) * 1991-06-25 1999-01-15 Genetics Inst Bmp-9 zusammensetzungen
KR100259827B1 (ko) * 1991-11-04 2000-06-15 브루스 엠. 에이센, 토마스 제이 데스로저 재조합 골형태 형성 단백질 헤테로다이머, 그 조성물 및 사용방법

Also Published As

Publication number Publication date
KR100361058B1 (ko) 2003-04-10
US5756308A (en) 1998-05-26
NO317800B1 (no) 2004-12-13
AU682238B2 (en) 1997-09-25
PT733108E (pt) 2001-08-30
JPH09506259A (ja) 1997-06-24
WO1995016034A1 (en) 1995-06-15
JP3704350B2 (ja) 2005-10-12
DK0733108T3 (da) 2001-06-18
US5804416A (en) 1998-09-08
CA2176943A1 (en) 1995-06-15
FI962349A (fi) 1996-07-16
FI962349A0 (fi) 1996-06-06
EP0733108A1 (en) 1996-09-25
DE69427093T2 (de) 2001-11-15
GR3035769T3 (en) 2001-07-31
DE69427093D1 (de) 2001-05-17
OA10294A (en) 1997-10-07
EP0733108B1 (en) 2001-04-11
FI118428B (fi) 2007-11-15
NO962303L (no) 1996-06-04
AU1209395A (en) 1995-06-27
CA2176943C (en) 2007-04-03
US5399677A (en) 1995-03-21
ATE200515T1 (de) 2001-04-15
NO962303D0 (no) 1996-06-04
ES2157313T3 (es) 2001-08-16

Similar Documents

Publication Publication Date Title
KR960706558A (ko) 골 발육 촉진성 단백질의 돌연변이체(mutants of bone morphogenetic proteins)
KR960701997A (ko) Bmp-10 조성물[bmp-10 compositions]
KR960701996A (ko) Bmp-11 조성물[bmp-11 compositions]
KR960706559A (ko) Bmp-12, bmp-13 및 이의 건(腱)-유도 조성물(bmp-12, bmp-13 andtendon-inducing compositions thereof)
CN101990546B (zh) 优化的重组生长因子蛋白纯化方法
EE9900581A (xx) LAG-3 pleissimisteisendid
KR960705850A (ko) 사람 쿠니즈형 프로테아제 저해인자(human kunitz-type protease inhibitors)
RU99114325A (ru) Днк, кодирующая мутантную изопропилмалат синтазу, микроорганизм-продуцент l-лейцина и способ получения l-лейцина
ES2063278T3 (es) Proteina morfogenetica de hueso.
CA2344974A1 (en) Chimaeric proteins between members of tgf-beta superfamily
KR940703914A (ko) 재조합 골 형태 형성 단백질 헤테로다이머, 그 조성물 및 사용방법(recombinant bone morphogenetic protein heterodimers, compositions and methods of use)
PT670898E (pt) Polipeptidos de multiplas mutacoes de interleucina-3 (il-3)
EA199700329A1 (ru) Новый белок и способ его получения
DE60110564D1 (en) Rna polymerase mutanten mit erhöhter thermostabilität
ATE380867T1 (de) Lactoferrinrezeptorgen von moraxella
DE69732631D1 (de) Metabotropische gaba(b) rezeptoren, rezeptoren-spezifische liganden und deren anwendungen
PE20231439A1 (es) Proteinas f de hmpv estabilizadas por prefusion
ATE255638T1 (de) Nukleotidsequenzen und gegen cycloheximid resistente proteine
HUP0001969A2 (hu) Az NNT-1 neurotróf faktor
Schwemmle et al. Binding of mRNA by an oligopeptide containing an evolutionarily conserved sequence from RNA binding proteins
ATE139570T1 (de) Expression signalpeptid-freier staphylokinasen
DK1117689T3 (da) Knoglemorfogenese-proteinantagonist baseret på det modne protein
Sieber et al. Monomeric and dimeric GDF-5 show equal type I receptor binding and oligomerization capability and have the same biological activity
EA200101066A1 (ru) Фрагменты вирусного белка 2 или 3 полиомавируса, используемые в качестве носителей для активных веществ
DE59207008D1 (de) Klonierung eines neuen mitgliedes der familie der fibroblasten-wachstumsfaktoren (fgf)-rezeptoren

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20051007

Year of fee payment: 4

LAPS Lapse due to unpaid annual fee