KR950704497A - 글리코실트랜스퍼라제 활성을 가진 단백질(Proteins Having Glycosyltransferase Activity) - Google Patents

글리코실트랜스퍼라제 활성을 가진 단백질(Proteins Having Glycosyltransferase Activity) Download PDF

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KR950704497A
KR950704497A KR1019950702127A KR19950702127A KR950704497A KR 950704497 A KR950704497 A KR 950704497A KR 1019950702127 A KR1019950702127 A KR 1019950702127A KR 19950702127 A KR19950702127 A KR 19950702127A KR 950704497 A KR950704497 A KR 950704497A
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protein
glycosyltransferase
dna molecule
glycosyltransferase activity
monarch
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KR1019950702127A
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에릭 지. 베르거
만프레트 바쩰레
스베토슬라브 엑스. 이와노우
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베르너 발데크
시바-가이기 아게
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Publication of KR950704497A publication Critical patent/KR950704497A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 글리코실트랜스퍼라제 활성을 가진 단백질, 글리코실트랜스퍼라제 활성을 가진 단백질을 코딩하는 재조합 DNA분자, 그러한 재조합 DNA분자로 이루어진 하이브리드 벡터, 재조합 DNA분자의 증식 및(또는)발현에 적합한 형질전환된 숙주, 및 그 단백질, DNA분자 및 숙주의 제조 방법에 관한 것이다.

Description

글리코실트랜스퍼라제 활성을 가진 단백질(Proteins Having Glycosyltransferase Activity)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 글리코실트랜스퍼라제의 동일하거나 또는 상이한 촉매 활성 도메인으로 이루어진 글리코실트랜스퍼라제 활성을 가진 단백질.
  2. 제1항에 있어서, 하이브리드 단백질인 단백질.
  3. 제2항에 있어서, 가용성 글리코실트랜스퍼라제에 연결된 막결합 또는 가용성 글리코실트랜스퍼라제로 이루어진 단백질.
  4. 제2항에 있어서, 유전학적으로 코딩된 아미노산으로 구성된 적합한 링커로 이루어진 단백질.
  5. 제2항에 있어서, 서열 동정 번호 5에 기재된 아미노산 서열을 가진 단백질 및 서열 동정 번호 7에 기재된 아미노산 서열을 가진 단백질로 이루어진 군으로부터 선택된 단백질.
  6. 상기 단백질이 발현을 가능케 하는 조건하에서 적합한 형질전환된 이스트 군주를 배양시키는 것으로 이루어지는 제2항에 따른 단백질의 제조 방법.
  7. 제2항에 따른 단백질을 코딩하는 DNA분자.
  8. 제7항에 따른 DNA분자로 이루어진 하이브리드 벡터.
  9. 제8항에 따른 하이브리드 벡터로 이루어진 형질전환된 이스트 군주.
  10. 글리코실화를 위한 제1항에 따른 단백질의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950702127A 1992-11-27 1993-11-15 글리코실트랜스퍼라제 활성을 가진 단백질(Proteins Having Glycosyltransferase Activity) KR950704497A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP92810924.8 1992-11-27
EP92810924 1992-11-27
PCT/EP1993/003194 WO1994012646A1 (en) 1992-11-27 1993-11-15 Proteins having glycosyltransferase activity

Publications (1)

Publication Number Publication Date
KR950704497A true KR950704497A (ko) 1995-11-20

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Application Number Title Priority Date Filing Date
KR1019950702127A KR950704497A (ko) 1992-11-27 1993-11-15 글리코실트랜스퍼라제 활성을 가진 단백질(Proteins Having Glycosyltransferase Activity)

Country Status (11)

Country Link
US (1) US5641668A (ko)
EP (1) EP0788548A1 (ko)
JP (1) JPH08503373A (ko)
KR (1) KR950704497A (ko)
AU (1) AU682837B2 (ko)
CA (1) CA2148929A1 (ko)
FI (1) FI952574A (ko)
MX (1) MX9307465A (ko)
NO (1) NO952039D0 (ko)
NZ (1) NZ258525A (ko)
WO (1) WO1994012646A1 (ko)

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US5770420A (en) * 1995-09-08 1998-06-23 The Regents Of The University Of Michigan Methods and products for the synthesis of oligosaccharide structures on glycoproteins, glycolipids, or as free molecules, and for the isolation of cloned genetic sequences that determine these structures
DE69738177T2 (de) 1996-08-02 2008-07-03 The Austin Research Institute, Heidelberg Verbesserte nukleinsäuren, welche für eine chimäre glycosyltransferase kodieren
US5945314A (en) * 1997-03-31 1999-08-31 Abbott Laboratories Process for synthesizing oligosaccharides
AU759792B2 (en) 1998-03-25 2003-05-01 Forschungszentrum Borstel Processive glycosyltransferase
US6586175B1 (en) 1998-07-28 2003-07-01 Dna Sciences Laboratories, Inc. Genotyping the human UDP-glucuronosyltransferase 2B7 (UGT2B7) gene
NZ512185A (en) 1998-12-09 2004-02-27 Dow Chemical Co A method for manufacturing glycoproteins having human- type glycosylation in plant cells
ATE478150T1 (de) 1999-10-26 2010-09-15 Stichting Dienst Landbouwkundi Säugetierartige glykosylierung in pflanzen
EP1356068B1 (en) 2001-01-19 2014-10-29 Phyton Holdings, LLC Method for secretory production of glycoprotein having human-type sugar chain using plant cell
ZA200700168B (en) 2001-10-10 2010-02-24 Novo Nordisk As Remodeling and glycoconjugation of peptides
ES2561985T3 (es) 2001-10-10 2016-03-01 Ratiopharm Gmbh Remodelación y glicoconjugación de anticuerpos
WO2003052088A2 (en) * 2001-12-18 2003-06-26 Bayer Healthcare Ag Regulation of human sialyltransferase
CA2478294C (en) 2002-03-19 2013-09-03 Plant Research International B.V. Gntiii (udp-n-acetylglucosamine:beta-d mannoside beta (1,4)-n-acetylglucosaminyltransferase iii) expression in plants
CN101613705A (zh) * 2002-03-19 2009-12-30 国际植物研究所 在植物中优化聚糖生成
WO2003093448A2 (en) * 2002-05-03 2003-11-13 Neose Technologies, Inc. Recombinant glycosyltransferase fusion proteins
WO2004009793A2 (en) 2002-07-23 2004-01-29 Neose Technologies, Inc. Synthesis of glycoproteins using bacterial gycosyltransferases
AR042145A1 (es) 2002-11-27 2005-06-08 Dow Agrociences Llc Produccion de inmunoglobulinas en plantas con una fucocilacion reducida
CA2522345A1 (en) 2003-04-09 2004-11-18 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
JP4892358B2 (ja) 2004-02-04 2012-03-07 ノヴォ ノルディスク アー／エス 哺乳動物グリコシルトランスフェラーゼをリフォールディングする方法
US8829276B2 (en) 2007-04-17 2014-09-09 Stichting Dienst Landbouwkundig Onderzoek Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
EP2824176A1 (en) * 2013-07-11 2015-01-14 Siamed'xpress Methods for producing sialylated therapeutic proteins
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN107429237B (zh) * 2014-12-22 2021-09-28 豪夫迈·罗氏有限公司 Cmp依赖性的唾液酸酶活性
EP3237607B1 (en) * 2014-12-22 2020-05-06 Veterinærinstituttet Salmon gill poxvirus
CN109312379B (zh) 2016-04-05 2022-04-12 科学与工业研究委员会 多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法

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AU4751990A (en) * 1988-12-13 1990-07-10 La Jolla Cancer Research Foundation Nucleotides encoding human b1, 4-galactosyltransferase and uses thereof
US5541083A (en) * 1989-10-24 1996-07-30 The Regents Of The University Of California Method for producing secretable glycosyltransferases and other golgi processing enzymes
US5032519A (en) * 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
CA2075949A1 (en) * 1990-02-14 1991-08-15 John B. Lowe Methods and products for the synthesis of oligosaccharide structures on glycoproteins, glycolipids, or as free molecules
EP0485532A1 (en) * 1990-04-27 1992-05-20 Biogen, Inc. Fucosyl transferases involved in adhesion molecule expression
DE4028800A1 (de) * 1990-09-11 1992-03-12 Behringwerke Ag Gentechnische sialylierung von glykoproteinen
GB2249096A (en) * 1990-10-11 1992-04-29 Ciba Geigy Saccharomyces cerevisiae strains lacking carboxypeptidase yscY activity for expression of proteins at high yields
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Also Published As

Publication number Publication date
NZ258525A (en) 1997-05-26
WO1994012646A1 (en) 1994-06-09
CA2148929A1 (en) 1994-06-09
US5641668A (en) 1997-06-24
NO952039L (no) 1995-05-23
EP0788548A1 (en) 1997-08-13
FI952574A0 (fi) 1995-05-26
AU5624294A (en) 1994-06-22
FI952574A (fi) 1995-05-26
JPH08503373A (ja) 1996-04-16
NO952039D0 (no) 1995-05-23
MX9307465A (es) 1994-08-31
AU682837B2 (en) 1997-10-23

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