KR950704476A - 생물학적 인자 및 신경 간세포(biological factors and neural stem cells) - Google Patents
생물학적 인자 및 신경 간세포(biological factors and neural stem cells)Info
- Publication number
- KR950704476A KR950704476A KR1019950701608A KR19950701608A KR950704476A KR 950704476 A KR950704476 A KR 950704476A KR 1019950701608 A KR1019950701608 A KR 1019950701608A KR 19950701608 A KR19950701608 A KR 19950701608A KR 950704476 A KR950704476 A KR 950704476A
- Authority
- KR
- South Korea
- Prior art keywords
- growth factor
- cells
- culture medium
- factor
- neural stem
- Prior art date
Links
- 210000001178 neural stem cell Anatomy 0.000 title claims abstract 8
- 239000003181 biological factor Substances 0.000 title 1
- 239000003102 growth factor Substances 0.000 claims abstract 18
- 210000004027 cell Anatomy 0.000 claims abstract 15
- 238000000034 method Methods 0.000 claims abstract 12
- 239000001963 growth medium Substances 0.000 claims abstract 10
- 210000002569 neuron Anatomy 0.000 claims abstract 6
- 239000002243 precursor Substances 0.000 claims abstract 6
- 210000004248 oligodendroglia Anatomy 0.000 claims abstract 3
- 230000002062 proliferating effect Effects 0.000 claims abstract 3
- 108010005939 Ciliary Neurotrophic Factor Proteins 0.000 claims 4
- 102100031614 Ciliary neurotrophic factor Human genes 0.000 claims 4
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 4
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 4
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 3
- 210000003494 hepatocyte Anatomy 0.000 claims 3
- 230000001537 neural effect Effects 0.000 claims 3
- 102000009024 Epidermal Growth Factor Human genes 0.000 claims 2
- 101800003838 Epidermal growth factor Proteins 0.000 claims 2
- 102000007072 Nerve Growth Factors Human genes 0.000 claims 2
- 210000004556 brain Anatomy 0.000 claims 2
- 229940116977 epidermal growth factor Drugs 0.000 claims 2
- 239000003900 neurotrophic factor Substances 0.000 claims 2
- 229930002330 retinoic acid Natural products 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 210000000130 stem cell Anatomy 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 230000009466 transformation Effects 0.000 claims 2
- 229960001727 tretinoin Drugs 0.000 claims 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical group C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 2
- ZHLCHIQFMOCSSC-UHFFFAOYSA-N 3-methyl-8-(2-methylpropyl)-7h-purine-2,6-dione Chemical compound CN1C(=O)NC(=O)C2=C1N=C(CC(C)C)N2 ZHLCHIQFMOCSSC-UHFFFAOYSA-N 0.000 claims 1
- 108010035532 Collagen Proteins 0.000 claims 1
- 102000008186 Collagen Human genes 0.000 claims 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 claims 1
- 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 claims 1
- 102000003971 Fibroblast Growth Factor 1 Human genes 0.000 claims 1
- 108010067306 Fibronectins Proteins 0.000 claims 1
- 102000016359 Fibronectins Human genes 0.000 claims 1
- 102000018997 Growth Hormone Human genes 0.000 claims 1
- 108010051696 Growth Hormone Proteins 0.000 claims 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims 1
- 102000015696 Interleukins Human genes 0.000 claims 1
- 108010063738 Interleukins Proteins 0.000 claims 1
- 108010085895 Laminin Proteins 0.000 claims 1
- 102000007547 Laminin Human genes 0.000 claims 1
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 1
- 108090000742 Neurotrophin 3 Proteins 0.000 claims 1
- 102000004230 Neurotrophin 3 Human genes 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 102000013275 Somatomedins Human genes 0.000 claims 1
- 102000005157 Somatostatin Human genes 0.000 claims 1
- 108010056088 Somatostatin Proteins 0.000 claims 1
- 108010055044 Tetanus Toxin Proteins 0.000 claims 1
- 230000003140 astrocytic effect Effects 0.000 claims 1
- 229920001436 collagen Polymers 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 claims 1
- 229960003957 dexamethasone Drugs 0.000 claims 1
- 239000000122 growth hormone Substances 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 208000032839 leukemia Diseases 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108010082117 matrigel Proteins 0.000 claims 1
- 229940053128 nerve growth factor Drugs 0.000 claims 1
- 229940032018 neurotrophin 3 Drugs 0.000 claims 1
- -1 neurotropin 4 Proteins 0.000 claims 1
- 108010055896 polyornithine Proteins 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 230000001902 propagating effect Effects 0.000 claims 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N retinoic acid group Chemical group C\C(=C/C(=O)O)\C=C\C=C(\C=C\C1=C(CCCC1(C)C)C)/C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims 1
- NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 claims 1
- 229960000553 somatostatin Drugs 0.000 claims 1
- 229940118376 tetanus toxin Drugs 0.000 claims 1
- 210000001130 astrocyte Anatomy 0.000 abstract 2
- 230000004069 differentiation Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/82—Translation products from oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0623—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01011—Phosphopyruvate hydratase (4.2.1.11), i.e. enolase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/46—Amines, e.g. putrescine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/148—Transforming growth factor alpha [TGF-a]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C12N2501/392—Sexual steroids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Developmental Biology & Embryology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Oncology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
본 발명에는 성상 세포, 희돌기 신경 교세포 또는 뉴론으로 분화되는 신경 간세포의 수를 증가시키는 방법이 기재되어 있다. 이 방법은 제1성장 인자를 가지고 있는 배양 배지에서 단리된 신경 간세포를 증식시킴으로써 선구 세포들을 제조하는 단계를 포함한다. 그런 다음 선구 세포들은 제1성장 인자가 없고, 제2성장 인자 또는 그것들의 조합을 함유하고 있는 제2배양 배지에서 성상 세포, 희돌기 신경 교세포 또는 뉴론으로 분화된다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (13)
- (a) 기중체의 조직으로부터 신경 간세포를 단리하는 단계; (b) 단리된 신경 간세포를 제1성장 인자를 가지고 있는 제1배양 배지에서 증식시켜서 선구 세포들을 제조하는 단계; 그리고 (C) 상기 선구세포들을, 실질적으로 상기 제1성장 인자가 없는, 최소한 제2성장 인자를 가지고 있는 제2배양 배지에서 배양함에 의해 선구 세포들을 분화시시킴으로써 분화된 세포를 제조하는 단계로 이루어지는, 신경 간세포로부터 분화된 세포를 제조하는 방법.
- 제1항에 있어서, 제1성장 인자가 표피 성장 인자인 것을 특징으로 하는 방법.
- 제1항에 있어서, 제2배양 배지가 혈청을 포함하는 것을 특징으로 하는 방법.
- 제1항에 있어서, 제2성장 인자가 산성 섬유아세포 성장 인자, 염기성 섬유아세포 성장인자, 모양체 신경 영양 인자, 신경 성장 인자, 뇌-유도 신경영양인자, 뉴로트로핀 3, 뉴로트로핀 4, 인터류킨, 백혈병 억제인자, 고리형 아데노신 모노포스페이트, 프르스콜린, 파상풍 독소, 고수준의 칼륨, 암피레굴린, 형질전환 성장인자 알파, 형질전환 성장 인자 베타, 인슐린-유사 성장 인자들, 덱사메타손, 이소부틸 3-메틸크산틴, 소마 토스타틴, 성장 호르몬, 레티노산 밋 혈소판-유도 성장 인자로 이루어지는 군으로부터 선택되는 것을 특징으로 하는 방법.
- 제1항에 있어서, 상기 분화된 세포들이 뉴론들이고 상기 제2성장 인자가 모양체 신경 영양 인자인 것을 특징으로 하는 방법.
- 제1항에 있어서, 상기 분화된 세포들이 성숙한 희돌기 신경 교세포이고 제2성장 인자가 모양체 신경 영양인자인 것을 특징으로 하는 방헙.
- 제1항에 있어서, 상기 분화된 세포들이 성상 세포이고, 상기 제2성장 인자가 모양체 신경 영양 인자이며, 상기 제2배양 배지가 실질적을 혈청을 함유하지 않는 것을 특징으로 하는 방법.
- 제1항에 있어서, 상기 분화된 세포들이 뉴론들이고 상기 제2성장 인자가 뇌-유도 신경 영양 인자인 것을 특징으로 하는 방법.
- 제1항에 있어서, 상기 분화된 세포들이 뉴론들이고 상기 제2성장 인자가 레티노산인 것을 특징으로 하는 방법.
- 제1항에 있어서, 상기 분화된 세포들이 뉴론들이고 상기 제2성장 인자가 염기성 섬유아세포 성장 인자인 것을 특징으로 하는 방법.
- (a) 기중체의 조직으로부터 신경 간세포를 단리하는 단계; (b) 단리된 신경 간세포를 염기성 섬유아세포 성장 인자를 함유하는 제1배양 배지에서 유지시키는 단계; 그리고 (C) 단리된 신경 간세포를 표피 성장 인자 및 염기성 섬유아세포 성장인자를 함유하는 제2배양 배지에서 증식시킴으로써 선구 세포들을 제조하는 단계로 이루어지는, 선구세포들의 제조방법.
- (a) 기증체의 조직으로부터 신경 간세포를 단리하는 단계; (b) 단리된 신경 간세포를 성장 인자를 가지고 있는 제1배양 배지에서 증식시킴으로써 선구 세포들을 제조하는 단계; 그리고 (C) 선구 세포들을 실질적으로 상기 제1성장 인자가 없는 제2배양 배지에서 기질과 접촉시키는 단계로 이루어지는, 신경 간세포로부터 분화된 세포를 제조하는 방법.
- 제12항에 있어서, 기질이 폴리-L-오르니틴, 콜라겐, 피브로넥틴, 라미닌 및 마트리겔로 이루어지는 군으로부터 선택되는 것을 특징으로 하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US96762292A | 1992-10-28 | 1992-10-28 | |
| US07/967622 | 1992-10-28 | ||
| PCT/CA1993/000456 WO1994010292A1 (en) | 1992-10-28 | 1993-10-27 | Biological factors and neural stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR950704476A true KR950704476A (ko) | 1995-11-20 |
Family
ID=25513066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019950701608A KR950704476A (ko) | 1992-10-28 | 1993-10-27 | 생물학적 인자 및 신경 간세포(biological factors and neural stem cells) |
Country Status (14)
| Country | Link |
|---|---|
| EP (2) | EP0669973B9 (ko) |
| JP (2) | JPH08502652A (ko) |
| KR (1) | KR950704476A (ko) |
| AT (2) | ATE234353T1 (ko) |
| AU (2) | AU5367694A (ko) |
| CA (1) | CA2148138C (ko) |
| DE (2) | DE69334344D1 (ko) |
| DK (1) | DK0669973T4 (ko) |
| ES (1) | ES2194016T5 (ko) |
| FI (1) | FI952022A (ko) |
| NO (1) | NO324412B1 (ko) |
| PT (1) | PT669973E (ko) |
| RU (1) | RU95115822A (ko) |
| WO (1) | WO1994010292A1 (ko) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5981165A (en) * | 1991-07-08 | 1999-11-09 | Neurospheres Holdings Ltd. | In vitro induction of dopaminergic cells |
| US5928947A (en) * | 1992-07-27 | 1999-07-27 | California Institute Of Technology | Mammalian multipotent neural stem cells |
| US5849553A (en) * | 1992-07-27 | 1998-12-15 | California Institute Of Technology | Mammalian multipotent neural stem cells |
| US6872699B2 (en) | 1992-11-13 | 2005-03-29 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften, E.V. | Truncated Flk-1 receptor protein, methods of use and a recombinant vector containing a nucleotide encoding the truncated Flk-1 protein |
| US6001654A (en) * | 1994-01-28 | 1999-12-14 | California Institute Of Technology | Methods for differentiating neural stem cells to neurons or smooth muscle cells using TGT-β super family growth factors |
| WO1996004368A1 (en) * | 1994-07-29 | 1996-02-15 | Dalhousie University | Propagation and inducible differentiation of human fetal central nervous system progenitor cells |
| KR970706490A (ko) * | 1994-09-23 | 1997-11-03 | 스코트 디. 코르맥 | 중추신경계 기능 및 기능부전의 실험실 모델(in vitro models of cns function and dysfunction) |
| KR970707272A (ko) * | 1994-11-14 | 1997-12-01 | 스코트 디. 코르맥 | 신경 간세포 증식 조절 방법(regulation of neural stem cell proliferation) |
| HUP9900243A2 (hu) * | 1995-05-24 | 1999-05-28 | Cellfactors Plc | Sejtdifferenciálódást befolyásoló hatóanyagok |
| FR2746109B1 (fr) | 1996-03-12 | 1998-04-17 | Rhone Poulenc Rorer Sa | Milieu pour la conservation de materiel biologique |
| US7544511B2 (en) | 1996-09-25 | 2009-06-09 | Neuralstem Biopharmaceuticals Ltd. | Stable neural stem cell line methods |
| US5753506A (en) * | 1996-05-23 | 1998-05-19 | Cns Stem Cell Technology, Inc. | Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals |
| US6969608B1 (en) | 1996-08-26 | 2005-11-29 | Mcgill University | Pharmaceuticals containing multipotential precursor cells from tissues containing sensory receptors |
| US6787355B1 (en) | 1996-08-26 | 2004-09-07 | Mcgill University | Multipotent neural stem cells from peripheral tissues and uses thereof |
| US6713247B1 (en) | 1996-09-03 | 2004-03-30 | Signal Pharmaceuticials, Inc. | Human CNS cell lines and methods of use therefor |
| CA2216439A1 (en) * | 1996-09-25 | 1998-03-25 | Derek Van Der Kooy | Pharmaceuticals containing retinal stem cells |
| KR20060002033A (ko) * | 1997-07-04 | 2006-01-06 | 유니버시티 오브 유타 리서치 파운데이션 | 계통이 예정된 신경세포 전구체 |
| US20020123143A1 (en) | 1997-08-22 | 2002-09-05 | Jean Toma | Multipotent stem cells from peripheral tissues and uses thereof |
| US5968829A (en) * | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
| US6165783A (en) | 1997-10-24 | 2000-12-26 | Neuro Spheres Holdings Ltd. | Erythropoietin-mediated neurogenesis |
| JP3291698B2 (ja) * | 1998-07-24 | 2002-06-10 | スティーブン・カーティン | 可変眼鏡用レンズ |
| US6787356B1 (en) | 1998-07-24 | 2004-09-07 | The United States Of America As Represented By The Department Of Health And Human Services | Cell expansion system for use in neural transplantation |
| WO2000006700A1 (en) * | 1998-07-29 | 2000-02-10 | Layton Bioscience, Inc. | Production and use of dopaminergic cells to treat dopaminergic deficiencies |
| SE9804064D0 (sv) * | 1998-11-25 | 1998-11-25 | A & Science Invest Ab | Medicinal product and method for treatment of conditions affecting neural stem cells or progenitor cells |
| US6291516B1 (en) | 1999-01-13 | 2001-09-18 | Curis, Inc. | Regulators of the hedgehog pathway, compositions and uses related thereto |
| EP1220681A2 (en) * | 1999-09-16 | 2002-07-10 | Prescient Neuropharma Inc. | Opaminergic neuronal survival-promoting factors and uses thereof |
| EP1226239A4 (en) | 1999-10-15 | 2003-02-12 | Advanced Cell Tech Inc | Process for the preparation of differentiated progenitor cells and embryonic stem cells with line defects |
| US7544509B2 (en) | 2000-01-24 | 2009-06-09 | Mcgill University | Method for preparing stem cell preparations |
| DE60142553D1 (de) | 2000-02-11 | 2010-08-26 | Children S Hospital Of Orange | Isolierung und transplantation von retinalen stammzellen |
| US7115653B2 (en) | 2000-03-30 | 2006-10-03 | Curis, Inc. | Small organic molecule regulators of cell proliferation |
| US8852937B2 (en) | 2000-03-30 | 2014-10-07 | Curis, Inc. | Small organic molecule regulators of cell proliferation |
| US6683108B1 (en) | 2000-03-30 | 2004-01-27 | Curis, Inc. | Agonists of hedgehog signaling pathways and uses related thereto |
| US6613798B1 (en) | 2000-03-30 | 2003-09-02 | Curis, Inc. | Small organic molecule regulators of cell proliferation |
| EP1318822A4 (en) * | 2000-07-27 | 2006-01-18 | Mclean Hospital Corp | CELL-IMPLANTATION THERAPY FOR NEUROLOGICAL DISORDERS OR DISEASES |
| US6908732B2 (en) | 2000-10-13 | 2005-06-21 | President & Fellows Of Harvard College | Compounds and methods for regulating cell differentiation |
| AU2002325712C1 (en) | 2001-08-30 | 2008-07-31 | Stem Cell Therapeutics Inc. | Differentiation of neural stem cells and therapeutic use theeof |
| CA2460184A1 (en) | 2001-09-14 | 2003-03-27 | Stem Cell Therapeutics Inc. | Prolactin induced increase in neural stem cell numbers and therapeutical use thereof |
| EP1435779A4 (en) | 2001-09-24 | 2005-06-01 | Sangamo Biosciences Inc | MODULATION OF STEM CELLS USING ZINC FINGER PROTEINS (ZFP) |
| CA2470853A1 (en) * | 2001-12-13 | 2003-06-19 | Japan Science And Technology Agency | Human cell culture medium and culture method |
| WO2003059276A2 (en) | 2002-01-14 | 2003-07-24 | The Board Of Trustees Of The University Of Illinois | Novel mammalian multipotent stem cells and compositions, methods of preparation and methods of administration thereof |
| EP2532241B1 (en) | 2002-02-15 | 2016-05-11 | Cornell Research Foundation, Inc. | Myelination of congenitally dysmyelinated forebrains using oligodendrocyte progenitor cells |
| CA2483311A1 (en) | 2002-04-22 | 2003-10-30 | Philip A. Beachy | Modulators of hedgehog signaling pathways, compositions and uses related thereto |
| JP4783013B2 (ja) * | 2002-07-30 | 2011-09-28 | ステム セル セラピューティクス インコーポレイテッド | 多能性神経幹細胞からの希突起神経膠細胞産生 |
| EP1546198A1 (en) * | 2002-07-31 | 2005-06-29 | Stem Cell Therapeutics Inc. | Method of enhancing neural stem cell proliferation, differentiation, and survival using pituitary adenylate cyclase activating polypeptide (pacap) |
| US20040214324A1 (en) | 2002-12-09 | 2004-10-28 | Ole Isacson | Dopaminergic neurons differentiated from embryonic cells for treating neurodegenerative diseases |
| US8293488B2 (en) | 2002-12-09 | 2012-10-23 | Neuralstem, Inc. | Method for screening neurogenic agents |
| EP1576134B1 (en) | 2002-12-09 | 2013-03-06 | Judith Kelleher-Andersson | Method for discovering neurogenic agents |
| US7459152B2 (en) | 2003-04-23 | 2008-12-02 | Rush University Medical Center | Erythropoietin administration to improve graft survival |
| JP4620052B2 (ja) | 2003-09-12 | 2011-01-26 | ステムセル テクノロジーズ インコーポレーティッド | 神経コロニー形成アッセイ法 |
| US20070269412A1 (en) | 2003-12-02 | 2007-11-22 | Celavie Biosciences, Llc | Pluripotent cells |
| CA2547827C (en) | 2003-12-02 | 2015-02-03 | Catholic Healthcare West | Compositions and methods for propagation of neural progenitor cells |
| WO2005077404A1 (en) | 2004-02-13 | 2005-08-25 | Stem Cell Therapeutics Corp. | Use of luteinizing hormone (lh) and chorionic gonadotropin (hcg) for proliferation of neural stem cells and neurogenesis |
| US7691629B2 (en) | 2004-11-17 | 2010-04-06 | Neuralstem, Inc. | Transplantation of human neural cells for treatment of neurodegenerative conditions |
| JP2009509943A (ja) | 2005-09-27 | 2009-03-12 | ステム セル セラピューティクス コーポレイション | プロラクチンにより制御される乏突起膠細胞前駆体細胞の増殖 |
| EP2004211A4 (en) | 2006-03-17 | 2010-07-07 | Stem Cell Therapeutics Corp | DOSEERSCHEMATA FOR LH OR HCG AND EPO FOR THE TREATMENT OF NEUROLOGICAL DISEASES |
| US20110218176A1 (en) | 2006-11-01 | 2011-09-08 | Barbara Brooke Jennings-Spring | Compounds, methods, and treatments for abnormal signaling pathways for prenatal and postnatal development |
| JP2008220205A (ja) * | 2007-03-09 | 2008-09-25 | Sumitomo Bakelite Co Ltd | 神経幹細胞凝集塊形成用容器、その製造方法、及び神経幹細胞凝集塊の作成方法。 |
| US8945919B2 (en) * | 2007-08-15 | 2015-02-03 | San Bio, Inc. | Methods and composition for treating neural degeneration |
| WO2009137674A2 (en) | 2008-05-08 | 2009-11-12 | University Of Rochester | Treating myelin diseases with optimized cell preparations |
| WO2010075500A1 (en) | 2008-12-23 | 2010-07-01 | Stemcells California, Inc | Target populations of oligodendrocyte precursor cells and methods of making and using same |
| EP2380972B1 (en) | 2010-04-19 | 2012-09-19 | Technische Universität Dresden | Methods and compositions for the expansion of somatic stem cells and progenitor cells |
| MY168084A (en) | 2010-07-28 | 2018-10-11 | Neuralstem Inc | Methods for treating and/or reversing neurodegenerative diseases and/or disorders |
| JP5867797B2 (ja) * | 2011-05-27 | 2016-02-24 | 国立大学法人京都大学 | ドーパミン産生神経の分化誘導用の細胞培養基材 |
| US10865383B2 (en) | 2011-07-12 | 2020-12-15 | Lineage Cell Therapeutics, Inc. | Methods and formulations for orthopedic cell therapy |
| CN104136601A (zh) | 2012-02-17 | 2014-11-05 | 斯格本斯眼科研究所 | 人视网膜祖细胞的表型谱 |
| JP6541577B2 (ja) | 2013-02-06 | 2019-07-10 | ユニバーシティー オブ ロチェスター | ミエリン障害の治療のための誘導多能性細胞由来オリゴデンドロサイト前駆細胞 |
| JP6983069B2 (ja) | 2014-10-20 | 2021-12-17 | ニューラルステム， インコーポレイテッド | 成長因子をコードする外因性ポリヌクレオチドを含む安定な神経幹細胞およびその使用の方法 |
| US9724432B2 (en) | 2015-04-30 | 2017-08-08 | University Of Rochester | Non-human mammal model of human degenerative disorder, uses thereof, and method of treating human degenerative disorder |
| US20180306780A1 (en) * | 2015-10-29 | 2018-10-25 | The Regents Of The University Of California | Astrocyte differentiation protocol |
| US11690876B2 (en) | 2017-05-10 | 2023-07-04 | University Of Rochester | Methods of treating neuropsychiatric disorders |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ226750A (en) * | 1987-10-29 | 1990-09-26 | Amrad Corp Ltd | Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene |
| AU6174490A (en) * | 1989-08-04 | 1991-03-11 | Board Of Regents, The University Of Texas System | Methods and compositions; purified preparation of neural progenitor regulatory factor |
| JP4416837B2 (ja) * | 1991-07-08 | 2010-02-17 | ニューロスフィアーズ ホウルディングス リミテッド | 試験管内で増殖し得る新規な成長因子応答性の前駆細胞 |
-
1993
- 1993-10-27 KR KR1019950701608A patent/KR950704476A/ko not_active Application Discontinuation
- 1993-10-27 AT AT93923994T patent/ATE234353T1/de active
- 1993-10-27 EP EP93923994A patent/EP0669973B9/en not_active Expired - Lifetime
- 1993-10-27 CA CA002148138A patent/CA2148138C/en not_active Expired - Fee Related
- 1993-10-27 DE DE69334344T patent/DE69334344D1/de not_active Expired - Lifetime
- 1993-10-27 ES ES93923994T patent/ES2194016T5/es not_active Expired - Lifetime
- 1993-10-27 DK DK93923994T patent/DK0669973T4/da active
- 1993-10-27 AT AT02018736T patent/ATE483795T1/de not_active IP Right Cessation
- 1993-10-27 EP EP02018736A patent/EP1298202B1/en not_active Expired - Lifetime
- 1993-10-27 DE DE69332759T patent/DE69332759T3/de not_active Expired - Lifetime
- 1993-10-27 JP JP6510503A patent/JPH08502652A/ja not_active Withdrawn
- 1993-10-27 WO PCT/CA1993/000456 patent/WO1994010292A1/en active IP Right Grant
- 1993-10-27 AU AU53676/94A patent/AU5367694A/en not_active Abandoned
- 1993-10-27 PT PT93923994T patent/PT669973E/pt unknown
-
1995
- 1995-04-27 FI FI952022A patent/FI952022A/fi unknown
- 1995-04-27 NO NO19951617A patent/NO324412B1/no unknown
- 1995-05-26 RU RU95115822/13A patent/RU95115822A/ru unknown
-
1997
- 1997-12-24 AU AU49241/97A patent/AU703729B2/en not_active Ceased
-
2006
- 2006-10-10 JP JP2006276639A patent/JP2007014352A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69334344D1 (de) | 2010-11-18 |
| AU4924197A (en) | 1998-03-12 |
| RU95115822A (ru) | 1997-03-20 |
| DK0669973T4 (da) | 2007-08-27 |
| NO324412B1 (no) | 2007-10-08 |
| EP0669973B2 (en) | 2007-04-18 |
| ATE234353T1 (de) | 2003-03-15 |
| EP0669973B1 (en) | 2003-03-12 |
| PT669973E (pt) | 2003-06-30 |
| NO951617L (no) | 1995-04-27 |
| CA2148138A1 (en) | 1994-05-11 |
| EP1298202A3 (en) | 2003-12-03 |
| FI952022A0 (fi) | 1995-04-27 |
| ES2194016T3 (es) | 2003-11-16 |
| NO951617D0 (no) | 1995-04-27 |
| JPH08502652A (ja) | 1996-03-26 |
| AU5367694A (en) | 1994-05-24 |
| DK0669973T3 (da) | 2003-07-14 |
| ES2194016T5 (es) | 2007-11-16 |
| EP1298202A2 (en) | 2003-04-02 |
| DE69332759D1 (de) | 2003-04-17 |
| DE69332759T3 (de) | 2008-01-24 |
| EP1298202B1 (en) | 2010-10-06 |
| DE69332759T2 (de) | 2003-10-16 |
| FI952022A (fi) | 1995-04-27 |
| ATE483795T1 (de) | 2010-10-15 |
| WO1994010292A1 (en) | 1994-05-11 |
| AU703729B2 (en) | 1999-04-01 |
| CA2148138C (en) | 2002-01-08 |
| JP2007014352A (ja) | 2007-01-25 |
| EP0669973A1 (en) | 1995-09-06 |
| EP0669973B9 (en) | 2007-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR950704476A (ko) | 생물학적 인자 및 신경 간세포(biological factors and neural stem cells) | |
| Sun et al. | Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture | |
| Heidemann et al. | Synergistic effects of cyclic AMP and nerve growth factor on neurite outgrowth and microtubule stability of PC12 cells. | |
| FI951677A (fi) | Remyelinaatio käyttäen neuraalisia kantasoluja | |
| CA2315538A1 (en) | Neural precursor cells, method for the production and use thereof in neural defect therapy | |
| Manthorpe et al. | Purification of mouse Schwann cells using neurite-induced proliferation in serum-free monolayer culture | |
| WO2000052143A2 (en) | The isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression | |
| SI2086332T1 (en) | From the bone marrow, mesenchymal stem cells are obtained as a source of neural precursors | |
| CN112553147A (zh) | 一种促进肌肉干细胞增殖的生长因子组合物及其应用 | |
| CN101294146A (zh) | 诱导神经干细胞分化的系统及诱导方法 | |
| CN101186900B (zh) | 一种由源于虹彩组织的神经干细胞生产网膜神经细胞的方法 | |
| CN104789531A (zh) | 一种将脐带间充质干细胞诱导分化成多巴胺能神经元的方法 | |
| Yang et al. | Effects of the CNTF-collagen gel-controlled delivery system on rat neural stem/progenitor cells behavior | |
| Schwarz et al. | Requirements for maintaining the embryonic state of avian tendon cells in culture | |
| Itoh | Culture of oligodendrocyte precursor cells (NG2+/O1−) and oligodendrocytes (NG2−/O1+) from embryonic rat cerebrum | |
| CN107164325B (zh) | MSCs来源的少突胶质细胞的制备方法及试剂盒 | |
| Barakat et al. | Carbonic anhydrase activity in primary sensory neurons: II. Influence of environmental factors on the phenotypic expression of the enzyme in dissociated cultures of chicken dorsal root ganglion cells | |
| CN104774807A (zh) | 将脐带间充质干细胞诱导分化成少突胶质细胞的方法 | |
| CN111334474A (zh) | 一种用于神经干细胞的体外移植培养方法 | |
| CN110684735A (zh) | 一种快速增殖神经干细胞培养基 | |
| CN115612667B (zh) | 一种诱导多能干细胞来源的前脑少突胶质细胞的制备方法 | |
| Yoshida et al. | Long-term effect of 2-deoxy-2-fluoroglucose on maintenance in culture of the neonatal B cell of rat | |
| CN107217036A (zh) | 一种神经细胞无血清培养基及其应用 | |
| CN113913383A (zh) | 一种胚胎神经干细胞体外培养基及培养方法 | |
| Hauschka et al. | Trophic Influences on Developing Muscle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E601 | Decision to refuse application |