KR880010123A - 신규 시토크롬 p-450 및 그의 제조방법 - Google Patents

신규 시토크롬 p-450 및 그의 제조방법 Download PDF

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KR880010123A
KR880010123A KR1019880000924A KR880000924A KR880010123A KR 880010123 A KR880010123 A KR 880010123A KR 1019880000924 A KR1019880000924 A KR 1019880000924A KR 880000924 A KR880000924 A KR 880000924A KR 880010123 A KR880010123 A KR 880010123A
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cytochrome
enzyme
sca
hydroxylation
streptomyces carbophilus
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KR1019880000924A
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KR960011917B1 (ko
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다쓰지 마쓰오까
스니찌 미야꼬시
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가와무라 요시부미
상꾜 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

신규 시토크롬 P-450 및 그의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 전기 영동적으로 순수한 시토크롬 P-450sca-1전기 영동적으로 순수한 시토크롬 P-450sca-2및 전기 영동적으로 순수한 시토크럼 P-450sca-3으로 이루어진 군으로부터 선택된 히드록실화 효소.
  2. 스트렙토미세스 카르보필루스 SANK 62585에 의해 생산 및 분리된 시토크롬 P-450 효소.
  3. 배지에서 스트렙토미세스 카르보필루스 SANK 62585를 배양하고, 상술한 배지에 상술한 효소의 생산을 유도할 수 있는 유도제를 첨가하고, 상술한 스트렙토미세스 카르보필루스 SANK 62585를 더 배양하고, 상술한 히드록실화 효소를 분리함을 특징으로 하는 히드록실화 효소의 제조방법.
  4. 효소를 사용하여 기질을 히드록실화하는 제조방법에 있어서, 스트렙토미세스 카르보필루스 SANK 62585에 의해 생산 및 분리된 시토크롬 P-450효소를 상술한 효소를 채택함을 특징으로 하는 개량방법.
  5. 제4항에 있어서, 상술한 히드록실화 효소를 시토크롬 P-450sca-1시토크롬 P-450sca-2및 시토크롬 P-450sca-3으로 이루어진 군에서 선택함을 특징으로 하는 개량방법.
  6. 시토크럼 P-450sca-2시토크롬 P-450sca-2및 시토크롬 P-450sca-3으로 이루어진 군에서 선택된 적어도 하나의 히드록실화 효소를 공급하고 ; 상술한 효소에 대한 기질을 공급하고 ; 상술한 히드록실화 효소를 사용하여 상술한 기질을 히드록실화하고 ; 및 히드록실화된 기질을 수득함을 특징으로 하는 히드록실화 방법.
  7. 제6항에 있어서, 배지에서 스트렙토미세스 카르보필루스 SANK 62585를 배양하고, 상술한 배지에 상술한 효소의 생산을 유도할 수 있는 유도체를 첨가하고, 상술한 스트렙토미세스 카르보필루스 SANK 62585를 더 배양하고, 상술한 히드록실화 효소를 분리함을 특징으로 하는 히드록실화 방법.
  8. 제7항에 있어서, 상술한 유도체 및 상술한 기질이 동일한 화합물임을 특징으로 하는 히드록실화 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880000924A 1987-02-02 1988-02-02 신규 시토크롬 p-450 및 그의 제조방법 KR960011917B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2195487 1987-02-02
JP21021787 1987-08-26
JP62-210217 1987-08-26
JP62-21954 1987-08-26

Publications (2)

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KR880010123A true KR880010123A (ko) 1988-10-07
KR960011917B1 KR960011917B1 (ko) 1996-09-04

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KR1019880000924A KR960011917B1 (ko) 1987-02-02 1988-02-02 신규 시토크롬 p-450 및 그의 제조방법

Country Status (11)

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EP (1) EP0281245B1 (ko)
KR (1) KR960011917B1 (ko)
AT (1) ATE108483T1 (ko)
AU (1) AU605543B2 (ko)
CA (1) CA1313637C (ko)
DE (1) DE3850589T2 (ko)
DK (1) DK175425B1 (ko)
ES (1) ES2059499T3 (ko)
FI (1) FI94647C (ko)
HK (1) HK1005747A1 (ko)
IE (1) IE63843B1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210136753A (ko) * 2020-05-08 2021-11-17 경북대학교 산학협력단 시토크롬 p450 활성 저해제 및 이의 용도

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212296A (en) * 1989-09-11 1993-05-18 E. I. Du Pont De Nemours And Company Expression of herbicide metabolizing cytochromes
WO1994026916A1 (en) * 1993-05-17 1994-11-24 University Of Toronto Innovations Foundation Monooxygenase-like activity of heme proteins
CA2176949A1 (en) * 1993-12-08 1995-06-15 Birgit Maria Koch Cytochrome p-450 monooxygenases
NZ299835A (en) * 1995-11-29 1997-08-22 Sankyo Co Actinomycete promoter for the expression of p-450 cytochromes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3682557D1 (de) * 1985-09-13 1992-01-02 Sankyo Co Hydroxy-ml-236b-derivate, deren herstellung und anwendung.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210136753A (ko) * 2020-05-08 2021-11-17 경북대학교 산학협력단 시토크롬 p450 활성 저해제 및 이의 용도

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Publication number Publication date
DK53488A (da) 1988-08-03
FI880458A (fi) 1988-08-03
KR960011917B1 (ko) 1996-09-04
IE63843B1 (en) 1995-06-14
EP0281245A3 (en) 1988-12-21
EP0281245B1 (en) 1994-07-13
ES2059499T3 (es) 1994-11-16
DK175425B1 (da) 2004-10-18
CA1313637C (en) 1993-02-16
IE880278L (en) 1988-08-02
FI94647B (fi) 1995-06-30
AU605543B2 (en) 1991-01-17
DK53488D0 (da) 1988-02-02
AU1117988A (en) 1988-08-04
FI94647C (fi) 1995-10-10
FI880458A0 (fi) 1988-02-02
DE3850589D1 (de) 1994-08-18
HK1005747A1 (en) 1999-01-22
EP0281245A2 (en) 1988-09-07
DE3850589T2 (de) 1995-04-27
ATE108483T1 (de) 1994-07-15

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