KR880002997A - 세포 배양방법 및 그의 기구 - Google Patents

세포 배양방법 및 그의 기구 Download PDF

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KR880002997A
KR880002997A KR870009410A KR870009410A KR880002997A KR 880002997 A KR880002997 A KR 880002997A KR 870009410 A KR870009410 A KR 870009410A KR 870009410 A KR870009410 A KR 870009410A KR 880002997 A KR880002997 A KR 880002997A
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container
semipermeable membrane
culture
cell culture
membrane container
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KR870009410A
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KR910007820B1 (ko
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가쯔도 와다나베
요시히꼬 나까무라
다까시 노도
마사이찌 야마무라
히도시 나까시마
가즈노리 이찌노헤
유끼다까 미노
가즈히로 니시지마
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가와노 다꾸미
가와스미 가가꾸 고오교오 가부시끼가이샤
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Priority claimed from JP61198898A external-priority patent/JPS6356275A/ja
Priority claimed from JP4674587U external-priority patent/JPH0353679Y2/ja
Priority claimed from JP62080991A external-priority patent/JPS63248382A/ja
Priority claimed from JP62172101A external-priority patent/JPH0620524B2/ja
Application filed by 가와노 다꾸미, 가와스미 가가꾸 고오교오 가부시끼가이샤 filed Critical 가와노 다꾸미
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    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/10—Mixers with rotating receptacles with receptacles rotated about two different axes, e.g. receptacles having planetary motion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/50—Mixers with shaking, oscillating, or vibrating mechanisms with a receptacle submitted to a combination of movements, i.e. at least one vibratory or oscillatory movement
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S604/00—Surgery
    • Y10S604/903—Medical container with material agitation means

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

내용 없음

Description

세포 배양방법 및 그의 기구
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 배양용기의 개략도,
제2도는 본 발명의 배양용기의 구체적인 실시예를 표시한 상세도,
제3도는 본 발명의 배양용기의 다른 실시예를 표시한 것으로, (A)는 조립분해도, (B)는 반단면 사시도.

Claims (10)

  1. 동물세포를 배양함에 있어서, 피배양물을 양분과 동시에 반투막 용기내에 봉입하여, 이 반투막 용기를 수납용기에 수납하는 동시에 반투막 용기의 막밖에 양분과 가스를 존재시켜 상기 반투막을 통하여 반투막 용기내의 피배양물 부유액과 막밖의 양분과의 농도구배에 의한 확산현상에 의하여 반투막 용기내의 피배양물을 고밀도 배양하는 것을 특징으로 하는 배양방법.
  2. 제1항에 있어서, 배양중 반투막과 그의 수납용기로 된 세포 배양용기에 회전 또는 요동을 주는 것을 특징으로 하는 세포 배양방법.
  3. 제2항에 있어서, 반투막 용기와 그의 수납용기로 된 세포 배양용기를 배양중에 배양시키는데 있어서, 이 세표 배양용기를 요동판에 장착한 상태로 요동판을 ti시에 요동판의 X축을 중심으로 하여 n회 상하로 움직인 후, ti+N시에 Y축을 중심으로 하여 n회 상하로 움직여서, 이 요동판의 중심점(O)의 높이(hO)를 기준으로 하여 요동판의 X축상에 있는 점(A, C)과 Y축상에 있는 점(B, D)의 높이 (hA, hC) 및 (hB, hD)를 임의로 상하로 움직임을 반복하면서 요동시키는 것을 특징으로 하는 세포 배양방법.
  4. 용기내에 피배양물을 수납하는 반투막 용기를 설치, 이 반투막 용기의 외축에 배양액과 가스의 수납부를 형성하는 동시에 이 수납부와 상기 반투막 용기에 각각 수납물의 출입가능한 연통구를 설치한 것을 특징으로 하는 세포 배양용기.
  5. 제4항에 있어서, 상기 반투막 용기가 망사상의 프로텍터로 덮혀져 있는 것을 특징으로 하는 세포 배양용기.
  6. 제4항에 있어서, 플라스틱제의 외백의 내부에, 반투막제의 내백이 수납되어 있는 것을 특징으로 하는 세포 배양용기.
  7. 제4항에 있어서, 상기 반투막의 공경은 반투막내의 세포가 투과하지 않고 배양액이나 가스가 투과하는 크기인 것을 특징으로 하는 세포 배양용기.
  8. 용기 내에 피배양물을 수납하는 반투막 용기를 설치, 이 반투막 용기의 외측에 배양액과 가스의 수납부를 형성하는 동시에, 이 수납부와 상기 반투막 용기에 각각 수납물의 출입가능한 연통구를 설치한 세포 배양용기와, 상기 외측의 용기에 배양액을 공급하기 위한 백과, 상기 반투막 용기내에 세포부유 배양액을 주입하는 기구와, 상기 반투막 용기내의 배양 후의 세포를 회수하기 위한 백과 상기 세포 배양용기와 상기 배양액 공급백 또는 상기 세포 회수백을 연결하는 튜우브와, 상기 외측의 용기 또는 반투막용기에 무균공기를 주입하는 기구로 된 것을 특징으로 하는 세포 배양장치.
  9. 용기 내에 피배양물을 수납하는 반투막 용기를 설치, 이 반투막 용기의 외측에 배양액과 가스의 수납부를 형성하는 동시에, 이 수납부와 상기 반투막 용기에 각각 수납물의 출입가능한 연통구를 설치, 세포 배양용기를 배양중에 회전시키기 위한 장치로서, 상기 세포 배양용기를 장착하는 회전판과, 이 회전판의 회전장치로 되고 상기 회전판에는 상기 배양용기의 고정구가 설치되어 있으며, 또한 이 회전판은 수평면에 대하여 경사되어 설치되어 있는 것을 특징으로 하는 회전 배양장치
  10. 용기 내에 피배양물을 수납하는 반투막 용기를 설치, 이 반투막 용기의 외측에 배양액과 가스의 수납부를 형성하는 동시에, 이 수납부와 상기 반투막 용기에 각각 수납물의 출입가능한 연통구를 설치, 세포 배양용기를 배양중에 요동시키기 위한 장치로서, 중심부가 회동가능하게 지지되어, 또한 상기 세포 배양용기를 장착하기 위한 요동판과 이 요동판의 X축 상으로 일단부가 회동가능하게 지지된 압압봉과 Y축 상에 일단부가 회동가능하게 지지된 압압봉과 이것들의 압압봉을 교대로 또는 동시에 상하로 시켜서 상기 요동판을 전후 좌우방향으로 요동시키기 위한 요동기구로 되는 요동 배양장치
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870009410A 1986-08-27 1987-08-27 세포 배양방법 및 그의 기구 KR910007820B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
JP61198898A JPS6356275A (ja) 1986-08-27 1986-08-27 細胞培養器具
JP46745 1987-03-31
JP4674587U JPH0353679Y2 (ko) 1987-03-31 1987-03-31
JP62080991A JPS63248382A (ja) 1987-04-03 1987-04-03 細胞培養装置
JP80991 1987-04-03
JP172101 1987-07-11
JP62172101A JPH0620524B2 (ja) 1987-07-11 1987-07-11 液体及び／又は固体入り容器の揺動装置
JP198898 1988-08-10

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KR880002997A true KR880002997A (ko) 1988-05-13
KR910007820B1 KR910007820B1 (ko) 1991-10-02

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KR1019870009410A KR910007820B1 (ko) 1986-08-27 1987-08-27 세포 배양방법 및 그의 기구

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US (2) US5057429A (ko)
EP (1) EP0258795B1 (ko)
KR (1) KR910007820B1 (ko)
AU (1) AU600968B2 (ko)
CA (1) CA1305934C (ko)
DE (1) DE3788026T2 (ko)

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EP0258795A2 (en) 1988-03-09
DE3788026T2 (de) 1994-04-21
KR910007820B1 (ko) 1991-10-02
EP0258795B1 (en) 1993-11-03
CA1305934C (en) 1992-08-04
AU7748287A (en) 1988-03-03
US5057429A (en) 1991-10-15
DE3788026D1 (de) 1993-12-09
US5071760A (en) 1991-12-10
EP0258795A3 (en) 1988-12-07

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