KR860008277A - 프로인슐린-양 물질의 정제방법 - Google Patents

프로인슐린-양 물질의 정제방법 Download PDF

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KR860008277A
KR860008277A KR1019860002611A KR860002611A KR860008277A KR 860008277 A KR860008277 A KR 860008277A KR 1019860002611 A KR1019860002611 A KR 1019860002611A KR 860002611 A KR860002611 A KR 860002611A KR 860008277 A KR860008277 A KR 860008277A
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proinsulin
support
impurity
mixture containing
substance
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KR1019860002611A
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KR890001243B1 (ko
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데니스 디마치 리차드
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일라이 릴리 앤드 캄파니
매리 앤 터커
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
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  • Diabetes (AREA)
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  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

프로인슐린-양 물질의 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 프로인슐린-양 물질을 함유하는 불순 혼합물을 pH 약 7 내지 약 10에서 역상 대공극성 아크길레이트 에스테르 공중합체 수지 지지체에 적용시킨 다음 : 이 지지체로부터 아세톤, 아세토니트릴 및 아세톤과 아세토니트릴의 혼합물중에서 선택한 유기 희석제 약 10용적% 내지 약 30용적%를 함유하는 pH 약 8 내지 약 11의 수성 용출제를 사용하여 프로인슐린-양 물질을 용출시킴을 특징으로 하여, 프로인슐린-양 물질을 함유하는 불순 혼합물로부터 불순물을 분리시키는 방법.
  2. 제1항에 있어서, 프로인슐린-양 물질이 인체 프로인슬린의 아미노산 서열에 상용하는 서열을 갖는 방법.
  3. 제2항에 있어서, 프로인슐린-양 물질이 프로인슐린에 대한 전구체인 방법.
  4. 제3항에 있어서, 프로인슐린-양 물질이 프로인슐린 S-설포네이트인 방법.
  5. 제1 내지 4항중 어느 한 항에 있어서, 대공극성 아크릴레이트 에스테르 공중합체 지지체가 XAD-7 또는 XAD-8인 방법.
  6. 제5항에 있어서, 프로인슐린-양 물질을 함유하는 불순 혼합물을 뱃치 조건하에서 처리하는 방법.
  7. 제5항에 있어서, 프로인슐린-양 물질을 함유하는 불순 혼합물을 크로마토그래피 칼럼 조건하에서 처리하는 방법.
  8. 제5항에 있어서, 프로인슐린-양 물질을 함유하는 불순 혼합물을 pH 약 8 내지 약 9에서 대공극성 아크킬레이트 에스테르 공중합체 지지체에 적용시키는 방법.
  9. 제8항에 있어서, 불순 혼합물을 지지체에 적용시킨 후, 용출시키기 전에 지지체를 pH 약 7 내지 약 8.5의 수성 완충액으로 세척하는 방법.
  10. 제9항에 있어서, 프로인슐린-양 물질을 pH 약 10.5의 수성 용출제를 사용하여 지지체로부터 용출시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860002611A 1985-04-08 1986-04-07 프로인슐린-양 물질의 정제방법 KR890001243B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US720,641 1985-04-08
US06/720,641 US4616078A (en) 1985-04-08 1985-04-08 Process for purifying proinsulin-like materials
US720641 1985-04-08

Publications (2)

Publication Number Publication Date
KR860008277A true KR860008277A (ko) 1986-11-14
KR890001243B1 KR890001243B1 (ko) 1989-04-28

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KR1019860002611A KR890001243B1 (ko) 1985-04-08 1986-04-07 프로인슐린-양 물질의 정제방법

Country Status (12)

Country Link
US (1) US4616078A (ko)
EP (1) EP0197764B1 (ko)
JP (1) JPH0796559B2 (ko)
KR (1) KR890001243B1 (ko)
AT (1) ATE91690T1 (ko)
AU (1) AU593242B2 (ko)
CA (1) CA1284249C (ko)
DE (1) DE3688712T2 (ko)
DK (1) DK173414B1 (ko)
HU (1) HU205138B (ko)
IE (1) IE60570B1 (ko)
IL (1) IL78373A (ko)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
US4764592A (en) * 1987-04-23 1988-08-16 Eli Lilly And Company Crystalline human proinsulin and process for its production
DK340189D0 (da) * 1989-07-10 1989-07-10 Jens Villadsens Fabrikker A S Fremgangsmaade til fremstilling af en fuldklaebet belaegning paa et underlag
US5071560A (en) * 1989-07-17 1991-12-10 Uop Process for purifying phenylalanine
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
US7741536B2 (en) 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
US20030204864A1 (en) * 2001-02-28 2003-10-30 Henry Daniell Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids
KR100253916B1 (ko) * 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
CN1305589A (zh) * 1998-04-17 2001-07-25 基因创新有限公司 采用还原剂的改进型免疫诊断分析
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
DE19838097A1 (de) * 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Verfahren zur chromatographischen Reinigung von Insulinen
EP1274846A4 (en) * 2000-03-01 2004-09-29 Univ Auburn PLASTIDE TRANSFORMATION VECTOR FOR THE EXPRESSION OF HUMAN PROTEINS IN PLANTS

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649456A (en) * 1969-09-08 1972-03-14 Rohm & Haas Separation of polypeptide substances with macroreticular resins
US3907676A (en) * 1970-07-28 1975-09-23 Novo Terapeutisk Labor As Process for purifying insulin
US3876623A (en) * 1973-05-09 1975-04-08 Lilly Co Eli Process for purifying insulin
AU5397579A (en) * 1978-12-26 1980-07-03 Eli Lilly And Company Purifying insulin
US4430266A (en) * 1980-11-28 1984-02-07 Eli Lilly And Company Process for producing an insulin precursor

Also Published As

Publication number Publication date
HUT40680A (en) 1987-01-28
DK173414B1 (da) 2000-10-02
HU205138B (en) 1992-03-30
JPS61236796A (ja) 1986-10-22
IL78373A0 (en) 1986-07-31
DK150586A (da) 1986-10-09
KR890001243B1 (ko) 1989-04-28
DE3688712T2 (de) 1993-11-25
IE860887L (en) 1986-10-08
EP0197764A3 (en) 1988-08-31
DK150586D0 (da) 1986-04-03
CA1284249C (en) 1991-05-14
EP0197764B1 (en) 1993-07-21
IL78373A (en) 1990-07-12
JPH0796559B2 (ja) 1995-10-18
AU593242B2 (en) 1990-02-08
US4616078A (en) 1986-10-07
AU5565586A (en) 1986-10-16
DE3688712D1 (de) 1993-08-26
IE60570B1 (en) 1994-07-27
EP0197764A2 (en) 1986-10-15
ATE91690T1 (de) 1993-08-15

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