CA1264148C - Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
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Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
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Publication number
CA1264148C
CA1264148CCA488273ACA488273ACA1264148CCA 1264148 CCA1264148 CCA 1264148CCA 488273 ACA488273 ACA 488273ACA 488273 ACA488273 ACA 488273ACA 1264148 CCA1264148 CCA 1264148C
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Canada
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monoclonal antibodies
hydroxylapatite
analysis
preparation
tion
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THE USE OF HYDROXYLAPATITE FOR THE ANALYSIS AND PREPARATION OF PURIFIED MONOCLONAL ANTIBODIES ABSTRACT OF THE DISCLOSURE A novel method is disclosed for the purifica-tion of monoclonal antibodies from antibodies of the same isotype but different light chain composition which are frequently present in the fluids in which such anti-bodies are generated. According to the method, the mixture is placed in a hydroxylapatite chromatography column and eluted with a buffer solution in a concentra-tion gradient elution. With the appropriate optimiza-tion of system parameters, a separation of antibodies effective for both analytical and preparative purposes is obtained.
CA000488273A1985-08-071985-08-07Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
Expired - LifetimeCA1264148A
(en)
Protein material, inhibin, purification method thereof, antibody to inhibin, non-therapeutic inhibin / antibody preparations and use for diagnostic and analytical purposes
Carrier for binding anti-phospholipid antibodies, its use in immunoassays and kits and blood protein which enhances binding ability of antiphospholipid antibody