CA1264148C - Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies - Google Patents

Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies

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Publication number
CA1264148C
CA1264148C CA488273A CA488273A CA1264148C CA 1264148 C CA1264148 C CA 1264148C CA 488273 A CA488273 A CA 488273A CA 488273 A CA488273 A CA 488273A CA 1264148 C CA1264148 C CA 1264148C
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CA
Canada
Prior art keywords
monoclonal antibodies
hydroxylapatite
analysis
preparation
tion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA488273A
Other languages
French (fr)
Other versions
CA1264148A (en
Inventor
Sheldon Craig Engelhorn
Hector Juarez-Salinas
Gary Steven Ott
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Publication date
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Priority to CA000488273A priority Critical patent/CA1264148A/en
Application granted granted Critical
Publication of CA1264148C publication Critical patent/CA1264148C/en
Publication of CA1264148A publication Critical patent/CA1264148A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

THE USE OF HYDROXYLAPATITE FOR THE ANALYSIS AND PREPARATION OF PURIFIED MONOCLONAL ANTIBODIES ABSTRACT OF THE DISCLOSURE A novel method is disclosed for the purifica-tion of monoclonal antibodies from antibodies of the same isotype but different light chain composition which are frequently present in the fluids in which such anti-bodies are generated. According to the method, the mixture is placed in a hydroxylapatite chromatography column and eluted with a buffer solution in a concentra-tion gradient elution. With the appropriate optimiza-tion of system parameters, a separation of antibodies effective for both analytical and preparative purposes is obtained.
CA000488273A 1985-08-07 1985-08-07 Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies Expired - Lifetime CA1264148A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA000488273A CA1264148A (en) 1985-08-07 1985-08-07 Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA000488273A CA1264148A (en) 1985-08-07 1985-08-07 Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies

Publications (2)

Publication Number Publication Date
CA1264148C true CA1264148C (en) 1990-01-02
CA1264148A CA1264148A (en) 1990-01-02

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ID=4131139

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000488273A Expired - Lifetime CA1264148A (en) 1985-08-07 1985-08-07 Hydroxylapatite for the analysis and preparation of purified monoclonal antibodies

Country Status (1)

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CA (1) CA1264148A (en)

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Publication number Publication date
CA1264148A (en) 1990-01-02

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