EP0197764A3 - Purifying proinsulin-like materials - Google Patents

Purifying proinsulin-like materials Download PDF

Info

Publication number
EP0197764A3
EP0197764A3 EP86302461A EP86302461A EP0197764A3 EP 0197764 A3 EP0197764 A3 EP 0197764A3 EP 86302461 A EP86302461 A EP 86302461A EP 86302461 A EP86302461 A EP 86302461A EP 0197764 A3 EP0197764 A3 EP 0197764A3
Authority
EP
European Patent Office
Prior art keywords
proinsulin
purifying
materials
acetone
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP86302461A
Other versions
EP0197764B1 (en
EP0197764A2 (en
Inventor
Richard Dennis Dimarchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Priority to AT86302461T priority Critical patent/ATE91690T1/en
Publication of EP0197764A2 publication Critical patent/EP0197764A2/en
Publication of EP0197764A3 publication Critical patent/EP0197764A3/en
Application granted granted Critical
Publication of EP0197764B1 publication Critical patent/EP0197764B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A process is provided for separating impurities from an impure mixture containing proinsulin-like material, which comprises: (1) applying said mixture to a reverse phase macroporous acrylate ester copolymer resin support at a pH of from about 7 to about 10; and (2) eluting said proinsulin-like material from said support with an aqueous eluant having a pH of from about 8 to about 11 and containing from about 10% to about 30% by volume of an organic diluent selected from the group consisting of acetone, scetonitrile, and a combination of acetone and acetonitrile.
EP86302461A 1985-04-08 1986-04-03 Purifying proinsulin-like materials Expired - Lifetime EP0197764B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT86302461T ATE91690T1 (en) 1985-04-08 1986-04-03 PURIFICATION OF PROINSULIN SUBSTANCES.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/720,641 US4616078A (en) 1985-04-08 1985-04-08 Process for purifying proinsulin-like materials
US720641 1985-04-08

Publications (3)

Publication Number Publication Date
EP0197764A2 EP0197764A2 (en) 1986-10-15
EP0197764A3 true EP0197764A3 (en) 1988-08-31
EP0197764B1 EP0197764B1 (en) 1993-07-21

Family

ID=24894751

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86302461A Expired - Lifetime EP0197764B1 (en) 1985-04-08 1986-04-03 Purifying proinsulin-like materials

Country Status (12)

Country Link
US (1) US4616078A (en)
EP (1) EP0197764B1 (en)
JP (1) JPH0796559B2 (en)
KR (1) KR890001243B1 (en)
AT (1) ATE91690T1 (en)
AU (1) AU593242B2 (en)
CA (1) CA1284249C (en)
DE (1) DE3688712T2 (en)
DK (1) DK173414B1 (en)
HU (1) HU205138B (en)
IE (1) IE60570B1 (en)
IL (1) IL78373A (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (en) * 1987-11-13 1989-05-24 Hoechst Ag METHOD FOR INSULATING AND CLEANING HIRUDINE
US4764592A (en) * 1987-04-23 1988-08-16 Eli Lilly And Company Crystalline human proinsulin and process for its production
DK340189D0 (en) * 1989-07-10 1989-07-10 Jens Villadsens Fabrikker A S PROCEDURE FOR PREPARING A COMPLETE COATING ON A SUBSTRATE
US5071560A (en) * 1989-07-17 1991-12-10 Uop Process for purifying phenylalanine
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
US7741536B2 (en) 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
US20030204864A1 (en) * 2001-02-28 2003-10-30 Henry Daniell Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids
KR100253916B1 (en) * 1997-12-29 2000-05-01 김충환 A process for preparing human proinsulin
DK1071955T4 (en) * 1998-04-17 2009-06-15 Innogenetics Nv Improved immuno-diagnostic assays using reducing agents
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
DE19838097A1 (en) * 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Process for the chromatographic purification of insulins
CA2402066C (en) * 2000-03-01 2015-09-29 Auburn University Plastid transformation vectors for expressing human proteins in plants

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649456A (en) * 1969-09-08 1972-03-14 Rohm & Haas Separation of polypeptide substances with macroreticular resins
US3907676A (en) * 1970-07-28 1975-09-23 Novo Terapeutisk Labor As Process for purifying insulin
US3876623A (en) * 1973-05-09 1975-04-08 Lilly Co Eli Process for purifying insulin
AU5397579A (en) * 1978-12-26 1980-07-03 Eli Lilly And Company Purifying insulin
US4430266A (en) * 1980-11-28 1984-02-07 Eli Lilly And Company Process for producing an insulin precursor

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 100, no. 13, 26th March 1984, page 82, abstract no. 96830c, Columbus, Ohio, US; J.E. BREWER et al.: "Fast systems for in-process and end-product quality control", & BIOTECH. 83 (Eighty-Three), PROC. INT. CONF. COMMER. APPL. IMPLIC. BIOTECHNOL., 1st 1983, 843-52 *
CHEMICAL ABSTRACTS, vol. 102, no. 1, 7th January 1985, page 64, abstract no. 665v, Columbus, Ohio, US; M. KNIP: "Analysis of pancreatic peptide hormones by reversed-phase high-performance liquid chromatography", & HORM. METAB. RES. 1984, 16(9), 487-91 *
CHEMICAL ABSTRACTS, vol. 88, no. 17, 24th April 1978, page 216, abstract no. 117165r, Columbus, Ohio, US; E.P. KROEFF et al.: "Investigation of the retention and separation of amino acids, peptides, and derivatives on porous copolymers by high performance liquid chromatography", & ANAL. CHEM. 1978, 50(3), 502-11 *
CHEMICAL ABSTRACTS, vol. 98, no. 11, 14th March 1983, page 67, abstract no. 83716k, Columbus, Ohio, US; A.U. PARMAN et al.: "Purification of porcine proinsulin by high-performance liquid chromatography", & J. CHROMATOGR. 1983, 256(2), 283-91 *

Also Published As

Publication number Publication date
JPH0796559B2 (en) 1995-10-18
AU593242B2 (en) 1990-02-08
HU205138B (en) 1992-03-30
KR890001243B1 (en) 1989-04-28
JPS61236796A (en) 1986-10-22
DE3688712T2 (en) 1993-11-25
KR860008277A (en) 1986-11-14
IE860887L (en) 1986-10-08
CA1284249C (en) 1991-05-14
DK150586D0 (en) 1986-04-03
EP0197764B1 (en) 1993-07-21
IL78373A0 (en) 1986-07-31
EP0197764A2 (en) 1986-10-15
ATE91690T1 (en) 1993-08-15
AU5565586A (en) 1986-10-16
DK173414B1 (en) 2000-10-02
US4616078A (en) 1986-10-07
IL78373A (en) 1990-07-12
DE3688712D1 (en) 1993-08-26
DK150586A (en) 1986-10-09
IE60570B1 (en) 1994-07-27
HUT40680A (en) 1987-01-28

Similar Documents

Publication Publication Date Title
EP0197764A3 (en) Purifying proinsulin-like materials
AU5151485A (en) A process for removal of hcn from gaseous streams
JPS5645483A (en) C-15003phm and its preparation
DE3064133D1 (en) Purification of human growth hormone
EP0197765A3 (en) Purifying growth hormone-like materials
ES8506462A1 (en) Process for separating a carboxylic acid of 1-8 carbon atoms from a mixture thereof with water and/or one or more other oxygenated aliphatic compounds.
JPS5223017A (en) Process for purification of (meth) acrylic acid or its esters
FR2575669B1 (en) PROCESS FOR THE SEPARATION OF URANIUM ISOTOPES BY OXIDOREDUCTION CHROMATOGRAPHY
FR2557872B1 (en) PROCESS FOR ISOLATING L-AMINO ACIDS FROM A REACTION MIXTURE
HU895637D0 (en) Process for adsorptive separation of para-xylene employing tetraline desorbent
DE3664039D1 (en) Process for the preparation of unsaturated compounds by elimination reaction
AU568694B2 (en) N-benzyl-n-phenyl-b-pyrrolidinyl-f-isobutoxy-propylamine
ES8607343A1 (en) Process for the separation of mixtures from insulin, derivatives of insulin and, if required, impurities.
AU7955687A (en) Method for purification of antibodies
JPS5747485A (en) Production of l-2-amino-4-methylphosphinobutyric acid
AU581446B2 (en) A process for obtaining uranium-bearing concentrate of high purity from liquors contaminated with zirconium
IL125414A (en) PROCESS FOR THE PURIFICATION OF 11beta-21-DIHYDROXY-2'-METHYL-5'betaH-PREGNA-1,4-DIENO [17,16-D] OXAZOLINE-3,20-DIONE
YU155789A (en) Process for adsorptive separation of para-xylene by using diethyltoluole
JPS54144389A (en) Purification of 7-aminocephem compound
DE69008369D1 (en) Separation of 1,2-dihydroxycyclohexa-3,5-diene compounds.
OA08918A (en) Process for the enrichment of canallite by flotation.
JPS5791739A (en) Purification and separation of vitamin b12 and adsorbing agent thereof
CS134184A1 (en) Method of organic substances' mixture adsorbing separation
CS557987A1 (en) Process for the purification of emulsions stabilized by nonionogened tenzides

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19860408

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH DE FR GB IT LI NL SE

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AT BE CH DE FR GB IT LI NL SE

17Q First examination report despatched

Effective date: 19910710

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE CH DE FR GB IT LI NL SE

REF Corresponds to:

Ref document number: 91690

Country of ref document: AT

Date of ref document: 19930815

Kind code of ref document: T

ET Fr: translation filed
REF Corresponds to:

Ref document number: 3688712

Country of ref document: DE

Date of ref document: 19930826

ITF It: translation for a ep patent filed

Owner name: MODIANO & ASSOCIATI S.R

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed
EAL Se: european patent in force in sweden

Ref document number: 86302461.8

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: AT

Payment date: 20010314

Year of fee payment: 16

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: SE

Payment date: 20010405

Year of fee payment: 16

Ref country code: FR

Payment date: 20010405

Year of fee payment: 16

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: BE

Payment date: 20010516

Year of fee payment: 16

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20010627

Year of fee payment: 16

REG Reference to a national code

Ref country code: GB

Ref legal event code: IF02

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20020403

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20020404

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20020430

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20020430

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20020430

EUG Se: european patent has lapsed

Ref document number: 86302461.8

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20021231

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20040312

Year of fee payment: 19

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20040316

Year of fee payment: 19

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20040430

Year of fee payment: 19

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES;WARNING: LAPSES OF ITALIAN PATENTS WITH EFFECTIVE DATE BEFORE 2007 MAY HAVE OCCURRED AT ANY TIME BEFORE 2007. THE CORRECT EFFECTIVE DATE MAY BE DIFFERENT FROM THE ONE RECORDED.

Effective date: 20050403

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20050403

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20051101

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20051101

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20050403

NLV4 Nl: lapsed or anulled due to non-payment of the annual fee

Effective date: 20051101