DE3688712D1 - PROINSULIN CLEANING. - Google Patents

PROINSULIN CLEANING.

Info

Publication number
DE3688712D1
DE3688712D1 DE8686302461T DE3688712T DE3688712D1 DE 3688712 D1 DE3688712 D1 DE 3688712D1 DE 8686302461 T DE8686302461 T DE 8686302461T DE 3688712 T DE3688712 T DE 3688712T DE 3688712 D1 DE3688712 D1 DE 3688712D1
Authority
DE
Germany
Prior art keywords
proinsulin
acetone
cleaning
support
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686302461T
Other languages
German (de)
Other versions
DE3688712T2 (en
Inventor
Richard Dennis Dimarchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of DE3688712D1 publication Critical patent/DE3688712D1/en
Application granted granted Critical
Publication of DE3688712T2 publication Critical patent/DE3688712T2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A process is provided for separating impurities from an impure mixture containing proinsulin-like material, which comprises: (1) applying said mixture to a reverse phase macroporous acrylate ester copolymer resin support at a pH of from about 7 to about 10; and (2) eluting said proinsulin-like material from said support with an aqueous eluant having a pH of from about 8 to about 11 and containing from about 10% to about 30% by volume of an organic diluent selected from the group consisting of acetone, scetonitrile, and a combination of acetone and acetonitrile.
DE86302461T 1985-04-08 1986-04-03 Purification of proinsulin substances. Expired - Fee Related DE3688712T2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/720,641 US4616078A (en) 1985-04-08 1985-04-08 Process for purifying proinsulin-like materials

Publications (2)

Publication Number Publication Date
DE3688712D1 true DE3688712D1 (en) 1993-08-26
DE3688712T2 DE3688712T2 (en) 1993-11-25

Family

ID=24894751

Family Applications (1)

Application Number Title Priority Date Filing Date
DE86302461T Expired - Fee Related DE3688712T2 (en) 1985-04-08 1986-04-03 Purification of proinsulin substances.

Country Status (12)

Country Link
US (1) US4616078A (en)
EP (1) EP0197764B1 (en)
JP (1) JPH0796559B2 (en)
KR (1) KR890001243B1 (en)
AT (1) ATE91690T1 (en)
AU (1) AU593242B2 (en)
CA (1) CA1284249C (en)
DE (1) DE3688712T2 (en)
DK (1) DK173414B1 (en)
HU (1) HU205138B (en)
IE (1) IE60570B1 (en)
IL (1) IL78373A (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (en) * 1987-11-13 1989-05-24 Hoechst Ag METHOD FOR INSULATING AND CLEANING HIRUDINE
US4764592A (en) * 1987-04-23 1988-08-16 Eli Lilly And Company Crystalline human proinsulin and process for its production
DK340189D0 (en) * 1989-07-10 1989-07-10 Jens Villadsens Fabrikker A S PROCEDURE FOR PREPARING A COMPLETE COATING ON A SUBSTRATE
US5071560A (en) * 1989-07-17 1991-12-10 Uop Process for purifying phenylalanine
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
US20030204864A1 (en) * 2001-02-28 2003-10-30 Henry Daniell Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids
US7741536B2 (en) 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
KR100253916B1 (en) * 1997-12-29 2000-05-01 김충환 A process for preparing human proinsulin
DE69920895T3 (en) * 1998-04-17 2009-09-03 N.V. Innogenetics S.A. IMPROVED IMMUNDIAGNOSTIC TESTS BY USING REDUCING AGENTS
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
DE19838097A1 (en) * 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Process for the chromatographic purification of insulins
EP1274846A4 (en) * 2000-03-01 2004-09-29 Univ Auburn Plastid transformation vectors for expressing human proteins in plants

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649456A (en) * 1969-09-08 1972-03-14 Rohm & Haas Separation of polypeptide substances with macroreticular resins
US3907676A (en) * 1970-07-28 1975-09-23 Novo Terapeutisk Labor As Process for purifying insulin
US3876623A (en) * 1973-05-09 1975-04-08 Lilly Co Eli Process for purifying insulin
AU5397579A (en) * 1978-12-26 1980-07-03 Eli Lilly And Company Purifying insulin
US4430266A (en) * 1980-11-28 1984-02-07 Eli Lilly And Company Process for producing an insulin precursor

Also Published As

Publication number Publication date
HUT40680A (en) 1987-01-28
IE860887L (en) 1986-10-08
ATE91690T1 (en) 1993-08-15
CA1284249C (en) 1991-05-14
EP0197764A3 (en) 1988-08-31
AU5565586A (en) 1986-10-16
EP0197764B1 (en) 1993-07-21
HU205138B (en) 1992-03-30
EP0197764A2 (en) 1986-10-15
IL78373A (en) 1990-07-12
IE60570B1 (en) 1994-07-27
DE3688712T2 (en) 1993-11-25
DK173414B1 (en) 2000-10-02
KR860008277A (en) 1986-11-14
DK150586A (en) 1986-10-09
US4616078A (en) 1986-10-07
IL78373A0 (en) 1986-07-31
JPS61236796A (en) 1986-10-22
JPH0796559B2 (en) 1995-10-18
DK150586D0 (en) 1986-04-03
KR890001243B1 (en) 1989-04-28
AU593242B2 (en) 1990-02-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee