JPS5625199A - Purification of coenzyme a - Google Patents
Purification of coenzyme aInfo
- Publication number
- JPS5625199A JPS5625199A JP9944479A JP9944479A JPS5625199A JP S5625199 A JPS5625199 A JP S5625199A JP 9944479 A JP9944479 A JP 9944479A JP 9944479 A JP9944479 A JP 9944479A JP S5625199 A JPS5625199 A JP S5625199A
- Authority
- JP
- Japan
- Prior art keywords
- coenzyme
- water
- unexchangeable
- ion
- porous resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Saccharide Compounds (AREA)
Abstract
PURPOSE: To obtain the titled compound in high purity and yield simply without the desalting step in a short operation time, by bringing crude coenzyme A into contact with an ion unexchangeable porous resin, and eluting the coezyme A with water.
CONSTITUTION: A culture fluid of Brevibacterium ammoniagenes IAM1641 (FERM-P No.1471) as a starting material is heated, treated with active carbon and an organic solvent, and vacuum-dried to give crude coenzyme A, which is dissolved in distilled water, reduced with 2-mercaptoethanol, passed through a column of an ion unexchangeable porous resin, e.g. Amberlite XAD-2, and then eluated with water of pH4. The components of the respective eluate fractions are separated and determined by paper chromatography to give an elution curve. Pure coenzyme A (CoA) is then obtained from the respective fractions, and concentrated to afford the aimed compound.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9944479A JPS5625199A (en) | 1979-08-06 | 1979-08-06 | Purification of coenzyme a |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9944479A JPS5625199A (en) | 1979-08-06 | 1979-08-06 | Purification of coenzyme a |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5625199A true JPS5625199A (en) | 1981-03-10 |
Family
ID=14247547
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9944479A Pending JPS5625199A (en) | 1979-08-06 | 1979-08-06 | Purification of coenzyme a |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5625199A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5929700A (en) * | 1982-08-13 | 1984-02-16 | Nippon Zeon Co Ltd | Purification of s-adenosyl-l-methionine |
-
1979
- 1979-08-06 JP JP9944479A patent/JPS5625199A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5929700A (en) * | 1982-08-13 | 1984-02-16 | Nippon Zeon Co Ltd | Purification of s-adenosyl-l-methionine |
JPS635039B2 (en) * | 1982-08-13 | 1988-02-01 | Nippon Zeon Co |
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