KR860008277A - Method for Purifying Proinsulin-Like Substances - Google Patents

Method for Purifying Proinsulin-Like Substances Download PDF

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Publication number
KR860008277A
KR860008277A KR1019860002611A KR860002611A KR860008277A KR 860008277 A KR860008277 A KR 860008277A KR 1019860002611 A KR1019860002611 A KR 1019860002611A KR 860002611 A KR860002611 A KR 860002611A KR 860008277 A KR860008277 A KR 860008277A
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South Korea
Prior art keywords
proinsulin
support
mixture containing
impurity
ester copolymer
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KR1019860002611A
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Korean (ko)
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KR890001243B1 (en
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데니스 디마치 리차드
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일라이 릴리 앤드 캄파니
매리 앤 터커
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

A process is provided for separating impurities from an impure mixture containing proinsulin-like material, which comprises: (1) applying said mixture to a reverse phase macroporous acrylate ester copolymer resin support at a pH of from about 7 to about 10; and (2) eluting said proinsulin-like material from said support with an aqueous eluant having a pH of from about 8 to about 11 and containing from about 10% to about 30% by volume of an organic diluent selected from the group consisting of acetone, scetonitrile, and a combination of acetone and acetonitrile.

Description

프로인슐린-양 물질의 정제방법Method for Purifying Proinsulin-Like Substances

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

프로인슐린-양 물질을 함유하는 불순 혼합물을 pH 약 7 내지 약 10에서 역상 대공극성 아크길레이트 에스테르 공중합체 수지 지지체에 적용시킨 다음 : 이 지지체로부터 아세톤, 아세토니트릴 및 아세톤과 아세토니트릴의 혼합물중에서 선택한 유기 희석제 약 10용적% 내지 약 30용적%를 함유하는 pH 약 8 내지 약 11의 수성 용출제를 사용하여 프로인슐린-양 물질을 용출시킴을 특징으로 하여, 프로인슐린-양 물질을 함유하는 불순 혼합물로부터 불순물을 분리시키는 방법.An impurity mixture containing a proinsulin-positive material is applied to a reverse phase macroporous acrylate ester copolymer resin support at pH about 7 to about 10 and then selected from acetone, acetonitrile and a mixture of acetone and acetonitrile from this support. Impurity mixtures containing a proinsulin-positive material, characterized in that the proinsulin-positive material is eluted with an aqueous eluent having a pH of about 8 to about 11 containing an organic diluent of about 10% to about 30% by volume. Method for separating impurities from 제1항에 있어서, 프로인슐린-양 물질이 인체 프로인슬린의 아미노산 서열에 상용하는 서열을 갖는 방법.The method of claim 1, wherein the proinsulin-like substance has a sequence compatible with the amino acid sequence of human proinsulin. 제2항에 있어서, 프로인슐린-양 물질이 프로인슐린에 대한 전구체인 방법.The method of claim 2, wherein the proinsulin-like substance is a precursor to proinsulin. 제3항에 있어서, 프로인슐린-양 물질이 프로인슐린 S-설포네이트인 방법.The method of claim 3, wherein the proinsulin-like substance is proinsulin S-sulfonate. 제1 내지 4항중 어느 한 항에 있어서, 대공극성 아크릴레이트 에스테르 공중합체 지지체가 XAD-7 또는 XAD-8인 방법.The method according to claim 1, wherein the macroporous acrylate ester copolymer support is XAD-7 or XAD-8. 제5항에 있어서, 프로인슐린-양 물질을 함유하는 불순 혼합물을 뱃치 조건하에서 처리하는 방법.The method of claim 5 wherein the impurity mixture containing proinsulin-like material is treated under batch conditions. 제5항에 있어서, 프로인슐린-양 물질을 함유하는 불순 혼합물을 크로마토그래피 칼럼 조건하에서 처리하는 방법.The method of claim 5 wherein the impure mixture containing proinsulin-like material is treated under chromatography column conditions. 제5항에 있어서, 프로인슐린-양 물질을 함유하는 불순 혼합물을 pH 약 8 내지 약 9에서 대공극성 아크킬레이트 에스테르 공중합체 지지체에 적용시키는 방법.The method of claim 5, wherein the impurity mixture containing proinsulin-positive material is applied to the macroporous acrylate ester copolymer support at a pH of about 8 to about 9. 7. 제8항에 있어서, 불순 혼합물을 지지체에 적용시킨 후, 용출시키기 전에 지지체를 pH 약 7 내지 약 8.5의 수성 완충액으로 세척하는 방법.The method of claim 8, wherein after applying the impure mixture to the support, the support is washed with an aqueous buffer of pH about 7 to about 8.5 before eluting. 제9항에 있어서, 프로인슐린-양 물질을 pH 약 10.5의 수성 용출제를 사용하여 지지체로부터 용출시키는 방법.The method of claim 9, wherein the proinsulin-like material is eluted from the support using an aqueous eluent having a pH of about 10.5. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019860002611A 1985-04-08 1986-04-07 Process for purifying proinsulin-like materials KR890001243B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US06/720,641 US4616078A (en) 1985-04-08 1985-04-08 Process for purifying proinsulin-like materials
US720641 1985-04-08
US720,641 1985-04-08

Publications (2)

Publication Number Publication Date
KR860008277A true KR860008277A (en) 1986-11-14
KR890001243B1 KR890001243B1 (en) 1989-04-28

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US (1) US4616078A (en)
EP (1) EP0197764B1 (en)
JP (1) JPH0796559B2 (en)
KR (1) KR890001243B1 (en)
AT (1) ATE91690T1 (en)
AU (1) AU593242B2 (en)
CA (1) CA1284249C (en)
DE (1) DE3688712T2 (en)
DK (1) DK173414B1 (en)
HU (1) HU205138B (en)
IE (1) IE60570B1 (en)
IL (1) IL78373A (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (en) * 1987-11-13 1989-05-24 Hoechst Ag METHOD FOR INSULATING AND CLEANING HIRUDINE
US4764592A (en) * 1987-04-23 1988-08-16 Eli Lilly And Company Crystalline human proinsulin and process for its production
DK340189D0 (en) * 1989-07-10 1989-07-10 Jens Villadsens Fabrikker A S PROCEDURE FOR PREPARING A COMPLETE COATING ON A SUBSTRATE
US5071560A (en) * 1989-07-17 1991-12-10 Uop Process for purifying phenylalanine
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
US7741536B2 (en) 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
US20030204864A1 (en) * 2001-02-28 2003-10-30 Henry Daniell Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids
KR100253916B1 (en) * 1997-12-29 2000-05-01 김충환 A process for preparing human proinsulin
TR200003024T2 (en) * 1998-04-17 2000-12-21 Innogenetics N.V. Immunodiagnostic assays developed using reducing agents.
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
DE19838097A1 (en) * 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Process for the chromatographic purification of insulins
AU2001276813A1 (en) * 2000-03-01 2001-10-08 Auburn University Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenics plastids

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649456A (en) * 1969-09-08 1972-03-14 Rohm & Haas Separation of polypeptide substances with macroreticular resins
US3907676A (en) * 1970-07-28 1975-09-23 Novo Terapeutisk Labor As Process for purifying insulin
US3876623A (en) * 1973-05-09 1975-04-08 Lilly Co Eli Process for purifying insulin
AU5397579A (en) * 1978-12-26 1980-07-03 Eli Lilly And Company Purifying insulin
US4430266A (en) * 1980-11-28 1984-02-07 Eli Lilly And Company Process for producing an insulin precursor

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AU593242B2 (en) 1990-02-08
IL78373A (en) 1990-07-12
IL78373A0 (en) 1986-07-31
HUT40680A (en) 1987-01-28
KR890001243B1 (en) 1989-04-28
JPH0796559B2 (en) 1995-10-18
DE3688712T2 (en) 1993-11-25
EP0197764A3 (en) 1988-08-31
DK150586A (en) 1986-10-09
DK150586D0 (en) 1986-04-03
IE60570B1 (en) 1994-07-27
IE860887L (en) 1986-10-08
AU5565586A (en) 1986-10-16
HU205138B (en) 1992-03-30
JPS61236796A (en) 1986-10-22
EP0197764A2 (en) 1986-10-15
DK173414B1 (en) 2000-10-02
ATE91690T1 (en) 1993-08-15
US4616078A (en) 1986-10-07
CA1284249C (en) 1991-05-14
EP0197764B1 (en) 1993-07-21
DE3688712D1 (en) 1993-08-26

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