GB1285024A - Process of purifying insulin - Google Patents

Process of purifying insulin

Info

Publication number
GB1285024A
GB1285024A GB1015772A GB1015772A GB1285024A GB 1285024 A GB1285024 A GB 1285024A GB 1015772 A GB1015772 A GB 1015772A GB 1015772 A GB1015772 A GB 1015772A GB 1285024 A GB1285024 A GB 1285024A
Authority
GB
United Kingdom
Prior art keywords
water
insulin
column
miscible organic
miscible
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1015772A
Inventor
Klaus Holger Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Terapeutisk Laboratorium AS
Original Assignee
Novo Terapeutisk Laboratorium AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Terapeutisk Laboratorium AS filed Critical Novo Terapeutisk Laboratorium AS
Priority to GB1015772A priority Critical patent/GB1285024A/en
Publication of GB1285024A publication Critical patent/GB1285024A/en
Priority to MY7700303A priority patent/MY7700303A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1285024 Insulin purification NOVO TERAPEUTISK LABORATORIUM AS 29 July 1969 [9 Aug 1968] 10157/72 Divided out of 1285023 Heading A5B Commercial or crude insulin is purified by applying a water-containing solution thereof having a pH of from 6-10 to a column of an anion exchanger and eluting the column with a water/water-miscible organic solvent mixture containing 20-70% by vol. of water and a pH of 6-10, collecting the eluate fractions which show essentially a single component when analysed by polyacrylamide gel electrophoresis and recovering the insulin therefrom. Suitable water-miscible organic solvents are: dioxane, tetramethyl urea, dimethyl formamide, water-miscible ketones and alkanols (the last being preferred). The eluent also contains a buffer maintaining the pH in the range 6-10, preferably 6.5-8.5.
GB1015772A 1968-08-09 1968-08-09 Process of purifying insulin Expired GB1285024A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB1015772A GB1285024A (en) 1968-08-09 1968-08-09 Process of purifying insulin
MY7700303A MY7700303A (en) 1968-08-09 1977-12-31 Process of purifying insulin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1015772A GB1285024A (en) 1968-08-09 1968-08-09 Process of purifying insulin

Publications (1)

Publication Number Publication Date
GB1285024A true GB1285024A (en) 1972-08-09

Family

ID=9962587

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1015772A Expired GB1285024A (en) 1968-08-09 1968-08-09 Process of purifying insulin

Country Status (2)

Country Link
GB (1) GB1285024A (en)
MY (1) MY7700303A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2173503A (en) * 1985-04-12 1986-10-15 Berlin Chemie Veb Purification of insulin
WO2000011030A2 (en) * 1998-08-24 2000-03-02 Aventis Pharma Deutschland Gmbh Method for chromatographically purifying insulins
WO2013048330A1 (en) * 2011-09-30 2013-04-04 Ge Healthcare Bio-Sciences Ab Method for purification of cleaved pro-insulin

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2173503A (en) * 1985-04-12 1986-10-15 Berlin Chemie Veb Purification of insulin
WO2000011030A2 (en) * 1998-08-24 2000-03-02 Aventis Pharma Deutschland Gmbh Method for chromatographically purifying insulins
WO2000011030A3 (en) * 1998-08-24 2000-06-02 Aventis Pharma Gmbh Method for chromatographically purifying insulins
AU756105B2 (en) * 1998-08-24 2003-01-02 Sanofi-Aventis Deutschland Gmbh Method for chromatographically purifying insulins
US6710167B1 (en) 1998-08-24 2004-03-23 Aventis Pharma Deutschland Gmbh Procedure for the chromatographic purification of insulins
KR100703578B1 (en) * 1998-08-24 2007-04-05 사노피-아벤티스 도이칠란트 게엠베하 Method for chromatographically purifying insulins
CZ300604B6 (en) * 1998-08-24 2009-06-24 Sanofi - Aventis Deutschland GmbH Chromatographic purification process of insulins
WO2013048330A1 (en) * 2011-09-30 2013-04-04 Ge Healthcare Bio-Sciences Ab Method for purification of cleaved pro-insulin
CN103827136A (en) * 2011-09-30 2014-05-28 通用电气健康护理生物科学股份公司 Method for purification of cleaved pro-insulin
CN103827136B (en) * 2011-09-30 2016-07-06 通用电气健康护理生物科学股份公司 Purify the insulinogenic method of cracking
US9409967B2 (en) 2011-09-30 2016-08-09 Ge Healthcare Bioprocess R&D Ab Method for purification of cleaved pro-insulin

Also Published As

Publication number Publication date
MY7700303A (en) 1977-12-31

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Legal Events

Date Code Title Description
416 Proceeding under section 16 patents act 1949
414F Notice of opposition given (sect. 14/1949)
414B Case decided by the comptroller ** grants allowed (sect. 14/1949)
4145 Application made to the patents appeal tribunal (sect. 14/1949)
414L Case decided on appeal ** grant allowed (sect. 14/1949)
PS Patent sealed
429A Application made for amendment of specification (sect. 29/1949)
429H Application (made) for amendment of specification now open to opposition (sect. 29/1949)
429D Case decided by the comptroller ** specification amended (sect. 29/1949)
SP Amendment (slips) printed
PCNP Patent ceased through non-payment of renewal fee