KR840008695A - 효모표현 시스템에 α-인자 서열을 이용하는 방법 - Google Patents

효모표현 시스템에 α-인자 서열을 이용하는 방법 Download PDF

Info

Publication number
KR840008695A
KR840008695A KR1019840002188A KR840002188A KR840008695A KR 840008695 A KR840008695 A KR 840008695A KR 1019840002188 A KR1019840002188 A KR 1019840002188A KR 840002188 A KR840002188 A KR 840002188A KR 840008695 A KR840008695 A KR 840008695A
Authority
KR
South Korea
Prior art keywords
yeast
protein
heterologous
dna sequence
microorganism
Prior art date
Application number
KR1019840002188A
Other languages
English (en)
Inventor
싱그 아전
Original Assignee
월터 에이치. 드레거
제넨테크 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 월터 에이치. 드레거, 제넨테크 인코포레이티드 filed Critical 월터 에이치. 드레거
Publication of KR840008695A publication Critical patent/KR840008695A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

효모표현 시스템에 α-인자 서열을 이용하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도:α-인자에 대한 유전자를 유리하는 데에 혼성화탐침으로서 사용된 합성 올리고뉴클레오티드 집합체의
구조.
제3도 및 4도:α-인자 유전자들의 뉴클레오티드 서열.

Claims (17)

  1. 효모 생물에 이종인 단백질을 암호화시키는 DNA 서열에 결합된 효모알파 인자용 프로모터의 DNA 서열로 되느 효모표현 매개체로 효모 미생물을 형질변이시키고, 이 형질변이시킨 미생물을 배양시키고, 이 배양체에서 단백질을 회수하는 공정으로 되는 효모 표현의 생성물로서 효모에 이종인 단백질을 얻는 방법.
  2. 번역 판독 후레임에서, 효모 미생물에 이종인 성숙 단백질을 암호화시키는 DNA 서열에 결합된 효모알파 인자의 프리-프로 펩티드의 상당 부분을 암호화 시킨 DNA 서열로 되는 표현 매개체로 효모 미생물을 형질변이시키고, 이 형질변이 시킨 미생물을 배양시키고, 이 배양체에서 단백질을 회수하는 공정으로 되는 효모 표현의 생성물로서 효모에 이종인 단백질을 얻는 방법.
  3. 번역 판독 후레임에서, 효모 미생물에 이종인 성숙단백질을 암호화시키는 DNA 서열에 결합된 효모알파 인자 유전자의 프리-프로 펩티드서열의 상단 부분에 결합된 프로모터의 DNA 서열로 되는 표현 매개체로 효모 미생물을 형질변이시키고, 이 형질변이시킨 미생물을 배양시키고, 단백질을 그의 기질배지에서 회수하는 공정으로 되는 효모 표현의 생성물로서 효모에 이종인 단백질을 얻는 방법.
  4. 번역 판독 후레임에서, 효모 미생물에 이종인 성숙 단백질을 암호화시키는 DNA 서열에 결합된 효모알파 인자의 프로-프로 펩티드의 상당 부분을 암호화 시키는 DNA 서열로 되는 표현 매개체로 효모미생물을 형질변이시키고, 이 형질변이시킨 미생물을 배양시키는 공정으로 되는 기질배지로 효모에 이종인 단백질을 분비시키는 방법.
  5. 제4항에 있어서, 상기 DNA 서열이 알파 인자 프로모터로 제어하는 방법.
  6. 효모 미생물에 이종인 단백질릉 함호화시키는 DNA 서열에 결합된 효모 알파 인자 유전자의 프로모터의 DNA 서열로 효모표현 매개체.
  7. 제6항에 있어서, 번역 판독 후레임 상류에서, 효모 미생물에 이종인 성숙 단백질을 암호화시키는 DNA 서열에 결합된 효모 알파 인자의 프리-프로 펩티드의 상당부분을 암호화시키는 DNA 서열을 포함하는 표현 매개체.
  8. 번역 판독 후레임에서, 효모 미생물에 이종인 성숙 단백질을 암호화시키는 DNA 서열에 결합된 효모알파 유전자의 프리-푸로 펩티드의 상당 부분을 암호화 시키는 DNA 서열로 되는 효모표현 매개체.
  9. 제6항 내지 제8항에 있어서, 이종 단백질 DNA를 암호화시킨 DNA가 인체 인터페론, 소인터페론,조직 플라스미노겐 활성인자 및 레닌으로 되는 군중에서 선택된 1개의 단백질을 암호화시킨 표현매개체.
  10. 제6항 내지 제8항에 있어서, 이종 단백질을 암화시키는 DNA가 인슐린과 같은 성장인자 인자인 표현매개체.
  11. 제6항 내지 제10항의 표현매개체로 형질변이시킨 효모 미생물.
  12. 제1항의 방법에 의하여 생성된 단백질.
  13. 제2항의 방법에 의하여 생성된 단백질.
  14. 제3항의 방법에 의하여 생성된 단백질.
  15. 제4항의 방법에 의하여 생성된 단백질.
  16. 효모 알파인자 DNA로 부터 유도된 N-종자 프리-서열을 가지는 표현 생성물로서 기질 배지에서 성숙이종 단백질을 생성할 수 있는 효모 미생물(여기서, 전술한 프리-서열을 처리하고, 전술한 단백질을 배지로 분비시킨다).
  17. 제16항에 있어서, 성숙 이종 단백질이 인체 인슐린과 같은 성장 인자인 미생물.
    ※ 참고사항 : 최초출원내용에 의하여 공개하는 것임.
KR1019840002188A 1983-04-25 1984-04-24 효모표현 시스템에 α-인자 서열을 이용하는 방법 KR840008695A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US48832383A 1983-04-25 1983-04-25
US06/488323 1983-04-25
US50135283A 1983-06-06 1983-06-06
US06/501352 1983-06-06
US50609883A 1983-06-20 1983-06-20
US06/506098 1983-06-20

Publications (1)

Publication Number Publication Date
KR840008695A true KR840008695A (ko) 1984-12-17

Family

ID=27413799

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840002188A KR840008695A (ko) 1983-04-25 1984-04-24 효모표현 시스템에 α-인자 서열을 이용하는 방법

Country Status (6)

Country Link
EP (1) EP0123544A3 (ko)
KR (1) KR840008695A (ko)
DK (1) DK204784A (ko)
ES (1) ES8603957A1 (ko)
IL (1) IL71621A0 (ko)
NZ (1) NZ207926A (ko)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK172738B1 (da) * 1983-04-07 1999-06-21 Chiron Corp Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden
DE3486216T2 (de) * 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
GB2177095B (en) * 1984-07-20 1988-05-25 Celltech Ltd Eucaryotic expression vectors
CA1281672C (en) * 1984-07-20 1991-03-19 Kim A. Nasmyth Vectors
EP0170266B1 (en) * 1984-07-30 1993-03-24 Wakunaga Seiyaku Kabushiki Kaisha Process for producing protein, and vector, recombinant dna and transformant used therefor
US5187263A (en) * 1984-10-12 1993-02-16 Zymogenetics, Inc. Expression of biologically active PDGE analogs in eucaryotic cells
US5498600A (en) * 1984-10-12 1996-03-12 Zymogenetics, Inc. Biologically active mosaic proteins
EP0487116B1 (en) * 1984-10-12 1999-12-29 ZymoGenetics, Inc. Biologically active PDGF analogs in eucaryotic cells
US5045633A (en) * 1985-02-25 1991-09-03 Zymogenetics, Inc. Expression of biologically active PDGF analogs in eucaryotic cells
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5821079A (en) * 1985-05-02 1998-10-13 Transgene S.A. Vectors for the expression and secretion of hirudin by transformed yeasts
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
DE3540075A1 (de) * 1985-11-12 1987-05-14 Boehringer Ingelheim Int Human-lysozym
US5024941A (en) * 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
US5013652A (en) * 1986-10-14 1991-05-07 Genex Corporation Composite yeast vectors
JP2777132B2 (ja) 1986-10-22 1998-07-16 カーレ・エンム・ガウトビク 微生物によるヒト副甲状腺ホルモンの生産
US5420242A (en) * 1986-10-22 1995-05-30 Kaare M. Gautvik Production of human parathyroid hormone from microorganisms
US5010010A (en) * 1986-10-22 1991-04-23 Selmer-Sande, A.S. Production of human parathyroid hormone from microorganisms
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
WO1988010308A1 (en) * 1987-06-24 1988-12-29 Whitehead Institute For Biomedical Research Pheromone-inducible yeast promoter
US5266474A (en) * 1987-06-24 1993-11-30 Genentech, Inc. Balanced inducible transcription system
US5063154A (en) * 1987-06-24 1991-11-05 Whitehead Institute For Biomedical Research Pheromone - inducible yeast promoter
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
SE459586B (sv) * 1987-09-14 1989-07-17 Mta Szegedi Biolog Koezponti Strukturgen som kodar foer autentiskt humant serum albumin och foerfarande foer dess framstaellning
US5879907A (en) * 1987-09-14 1999-03-09 Skandigen Ab Artificial gene coding for authentic human serum albumin, use thereof and method
ZA889415B (en) * 1987-12-18 1989-09-27 Chiron Corp Compositions and method for recombinant production of crotalidus venum fibrolase
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
JP3092811B2 (ja) * 1988-07-23 2000-09-25 デルタ バイオテクノロジー リミテッド ペプチドおよびｄｎａ配列
JPH0671434B2 (ja) * 1989-09-18 1994-09-14 株式会社ミドリ十字 ヒト血清アルブミンの製造方法
WO1992005244A1 (en) 1990-09-13 1992-04-02 Duke University Expression of g protein coupled receptors in yeast
FR2669931B1 (fr) * 1990-11-29 1995-03-31 Transgene Nouveaux variants derives des interferons-alpha et leur procede de production.
WO1992009691A1 (fr) * 1990-11-29 1992-06-11 Institut National De La Recherche Agronomique - I.N.R.A. Nouveaux variants derives des interferons de type i, leur procede de production, et leurs applications
JPH0671432B2 (ja) * 1991-03-20 1994-09-14 株式会社ミドリ十字 ヒト血清アルブミンの製造方法
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide
JPH07308199A (ja) 1994-05-18 1995-11-28 Green Cross Corp:The ヒト血清アルブミンの製造方法
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
CN1182451A (zh) 1995-03-17 1998-05-20 诺沃挪第克公司 新的内切葡聚糖酶
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
DK1975177T3 (da) 1996-03-01 2011-07-25 Novo Nordisk As Appetitundertrykkende peptid, formuleringer dermed og anvendelse deraf
JP2001501097A (ja) * 1996-09-24 2001-01-30 ケイダス ファーマスーティカル コーポレイション レセプターエフェクターを同定する方法及び組成物
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
SE9702401D0 (sv) 1997-06-19 1997-06-19 Astra Ab Pharmaceutical use
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
EP2267026A1 (en) 2000-04-12 2010-12-29 Human Genome Sciences, Inc. Albumin fusion proteins
PL371800A1 (en) 2001-03-22 2005-06-27 Novo Nordisk Health Care Ag Coagulation factor vii derivatives
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
ES2376694T3 (es) 2001-09-27 2012-03-16 Novo Nordisk Health Care Ag Polipã‰ptidos del factor vii de coagulaciã“n humano.
PT1463751E (pt) 2001-12-21 2013-08-26 Human Genome Sciences Inc Proteínas de fusão de albumina
GB0217033D0 (en) 2002-07-23 2002-08-28 Delta Biotechnology Ltd Gene and polypeptide sequences
CA2925807C (en) 2002-09-13 2020-09-15 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
CN101870729A (zh) 2003-09-09 2010-10-27 诺和诺德医疗保健公司 凝固因子ⅶ多肽
US8034326B2 (en) 2005-04-18 2011-10-11 Novo Nordisk A/S IL-21 variants
US20090055942A1 (en) 2005-09-14 2009-02-26 Novo Nordisk Healthcare A/G Human Coagulation Factor VII Polypeptides
DK2615108T3 (en) 2006-09-08 2017-01-30 Ambrx Inc Modified human plasma polypeptide or fc scaffolds and their applications
CA2724052A1 (en) 2008-05-15 2009-11-19 R-Tech Ueno, Ltd. Pharmaceutical composition for the treatment of dry eye and/or corneal and conjunctival lesion
US20120107267A1 (en) 2009-03-11 2012-05-03 Novo Nordisk A/S Interleukin-21 variants having antagonistic binding to the il-21 receptor
EP3395828A1 (en) 2009-07-17 2018-10-31 Rigshospitalet Inhibitors of complement activation
WO2011067283A1 (en) 2009-12-01 2011-06-09 Novo Nordisk A/S Novel peptidyl alpha-hydroxyglycine alpha-amidating lyases
WO2011107591A1 (en) 2010-03-05 2011-09-09 Rigshospitalet Chimeric inhibitor molecules of complement activation
JP6517018B2 (ja) 2012-02-09 2019-05-22 ヴァー２・ファーマシューティカルズ・アンパルトセルスカブＶＡＲ２ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ ＡｐＳ コンドロイチン硫酸グリカンのターゲティング
CN104704118A (zh) 2012-10-15 2015-06-10 诺和诺德保健Ag（股份有限公司） 凝血因子vii多肽
EP3253779A1 (en) * 2015-02-06 2017-12-13 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
CA2979410A1 (en) 2015-03-27 2016-10-06 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
EA037848B1 (ru) 2016-07-14 2021-05-27 Общество С Ограниченной Ответственностью "Биохимический Агент" Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
CA3032736A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
EP3821244A1 (en) 2018-07-13 2021-05-19 Varct Diagnostics ApS Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa
WO2022049409A1 (en) 2020-09-02 2022-03-10 Sia Terragen Express diagnosticum for sars-cov-2

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0116201B1 (en) * 1983-01-12 1992-04-22 Chiron Corporation Secretory expression in eukaryotes
DK172738B1 (da) * 1983-04-07 1999-06-21 Chiron Corp Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden
WO1984004330A1 (en) * 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
DE3486216T2 (de) * 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
US4588684A (en) * 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
JPS59227899A (ja) * 1983-05-25 1984-12-21 チロン・コ−ポレイシヨン 成長ホルモン放出因子の製造方法
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
WO1985002200A1 (en) * 1983-11-14 1985-05-23 Chiron Corporation Interleukin-2 production using cloned genes for interleukin-2 and yeast alpha-factor
WO1985004870A1 (en) * 1984-04-19 1985-11-07 Biotechnology Research Partners, Ltd. Novel atrial natriuretic/vasodilator polypeptides

Also Published As

Publication number Publication date
ES8603957A1 (es) 1986-01-16
ES531898A0 (es) 1986-01-16
IL71621A0 (en) 1984-07-31
DK204784D0 (da) 1984-04-24
EP0123544A3 (en) 1986-07-16
DK204784A (da) 1984-10-26
EP0123544A2 (en) 1984-10-31
NZ207926A (en) 1988-04-29

Similar Documents

Publication Publication Date Title
KR840008695A (ko) 효모표현 시스템에 α-인자 서열을 이용하는 방법
ATE105858T1 (de) Dna-moleküle, die für fmdh-kontrollabschnitte und strukturgene für ein protein mit fmdh-aktivität kodieren, sowie deren anwendung.
KR830005350A (ko) 표면 결합체용 융합단백질 및 진핵 단백질 제조방법
KR880014110A (ko) 인체 프로아포지방단백질 a-i의 발현
KR840002031A (ko) Dna, 재조합 dna, 이들을 갖는 숙주 및 폴리펩타이드를 제조하는 방법
ATE145065T1 (de) Verfahren zur bestimmung der anwesenheit von diabetes assoziierten- oder inselzellen assoziierten antikörpers
ATE189264T1 (de) Chimärisches oligonukleotid und dessen verwendung zur herstellung von nukleinsäure-transkripten
ATE414162T1 (de) Verfahren und materialien zur herstellung von glucosamin
KR840008697A (ko) 상동 효모 신호를 이용한 이종단백질의 합성, 분비 및 표현매개체 제조방법
KR920702417A (ko) Tcf-ii의 아미노산 배열을 코드하는 dna를 함유한 플라스미드, 형질전환세포, 및 이것을 사용하여 생리 활성물질을 생산하는 방법
KR950703642A (ko) 만누로난 씨(c)-5-에피머라제를 암호화하는 서열을 포함하는 디 엔 에이(dna) 화합물(dna compounds comprising sequences encoding mannuronan c-5-epimerase)
KR870700095A (ko) AraB 프로모터 및 미생물학적 방법에 의해 세크로핀을 포함한 폴리펩타이드를 제조하는 방법
ATE542913T1 (de) Promotorvarianten zur expression von genen in einer pilzzelle
KR890700675A (ko) 새로운 단백질 분해 효소
PT80542A (de) Gentechnologisches verfahren zur herstellung von human-interleukin-2 und mittel zur durchfuehrung dieses verfahrens
ES2003314A4 (es) Enzimas amiloliticas que producen microorganismos construidos por tecnologia adn recombinante y su uso para procedimientos de fermentacion.
ATE218162T1 (de) Ketogruppe-einführendes enzym, dafür kodierende dna und verfahren zur herstellung von ketocarotenoid
DE68922790D1 (de) Verfahren zur Herstellung eines Proteins durch Hefe mit Hilfe eines induzierbaren Systems, Vektoren und entsprechende transformierte Stämme.
DE59109155D1 (de) Verfahren zur herstellung von vakzinen und ihre verwendung
Kilian et al. Cell wall ultrastructure of strains of Haemophilus ducreyi and Haemophilus piscium
CN113564195B (zh) 一种果糖胺脱糖酶毕赤酵母表达载体、基因工程菌及构建方法和蛋白表达方法
JP2002176979A (ja) セルロース結合ドメインを細胞表層に提示する形質転換酵母
Jenkins Recombinant paper plasmids
Mizrahi Biologicals produced from animal cells in culture—an overview
DE69427403T2 (de) Peptide-familie, genannt xenoxine

Legal Events

Date Code Title Description
A201 Request for examination
SUBM Surrender of laid-open application requested