KR830007820A - 미생물 배양제어방법 및 그 장치 - Google Patents

미생물 배양제어방법 및 그 장치 Download PDF

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KR830007820A
KR830007820A KR1019810004438A KR810004438A KR830007820A KR 830007820 A KR830007820 A KR 830007820A KR 1019810004438 A KR1019810004438 A KR 1019810004438A KR 810004438 A KR810004438 A KR 810004438A KR 830007820 A KR830007820 A KR 830007820A
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South Korea
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carbon dioxide
partial pressure
gas
culture
oxygen
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KR1019810004438A
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English (en)
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KR870001649B1 (ko
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노리오 시미즈
데쓰오 야마구찌
세쓰오 사이또오
마사오 우에노
요오지 오다와라
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미다 가쓰시게
가부시기가이샤 히다찌 세이사꾸쇼
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Priority claimed from JP16524080A external-priority patent/JPS6018389B2/ja
Priority claimed from JP56006332A external-priority patent/JPS6018390B2/ja
Priority claimed from JP2993181A external-priority patent/JPS6015306B2/ja
Application filed by 미다 가쓰시게, 가부시기가이샤 히다찌 세이사꾸쇼 filed Critical 미다 가쓰시게
Publication of KR830007820A publication Critical patent/KR830007820A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/813—Continuous fermentation

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  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
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  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

내용 없음

Description

미생물 배양제어방법 및 그장치
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 탄산가스 생성량과 균체증식량과의 관계를 나타낸 그래프.
제4도는 본원 발명장치의 일실시 예양태를 나타낸 모식도.
제6도는 본원 발명에 사용하는 배양제어장치의 일구체예를 나타낸 모식도.

Claims (8)

  1. 배양조내의 압력, 배기가스유량 및 배기가스중의 탄산가스농도를 측정함으로써 배양조내의 탄산가스분압과, 미생물이 생성하는 탄산가스량을 계산하고, 이탄산가스분압과 탄산가스생성량에서 균체증식량을 산출하여 배양액중의 균체량을 산출하며, 이 산출된 균체량에 응해서 기질공급량을 제어하는 것을 특징으로하는 미생물배양 제어방법.
  2. 미생물을 공기 또는 산소부화가스를 사용하여 배양할 경우에 용존산소농도의 제어도 조합(組合)해서 하는 특허청구의 범위 1기재의 미생물 배양 제어방법.
  3. 수율과 탄산가스분압과의 관계에 있어서의 탄산가스 분압의 임계치와 용존산소량과의 상대관계를 산소부화가스의 통기량 그 산소농도 및 교반기 회전수의 조합에 의해 제어하는 것을 특징으로하는 특허청구의 범위 2기재의 미생물 배양제어방법.
  4. 탄산가스분압의 임계치와 용존산소량과의 상대관계를 산소부화의 통기량, 그 산소농도, 교반기회전수 및 조내압력의 조합에 의해 제어하는 특허청구의 범위 3기재의 미생물 배양제어방법.
  5. 미생물이 빵효모인 특허청구의 범위 1 또는 2기재의 미생물 배양 제어방법.
  6. 빵 효모 배양시에 탄산가스분압을 0.2기압 이하, 용존산소량을 0.2~5ppm으로 유지하는 것을 특징으로하는 특허청구의 범위 5기재의 미생물 배양제어방법.
  7. 미생물을 호기적(好氣的)으로 배양할 때에 교반기회전수, 통기 가스중의 산소분압 및 통기량을 사용하여 용존산소농도를 제어하는 미생물배양제어방법에 있어서 배양액의 용존산소농도가 설정치보다 저하했을 경우는 제1단계로서 교반기회전수를 올리며, 교반기회전수가 설정상한치로 되었을 경우는 제2단계로서 통기가스중의 산소분압을 올리고, 통기가스중의 산소분압이 설정상한치로 되었을 경우는 제3단계로서 통기량을 올리며, 반대로 배양액의 용존산소농도가 설정치보다 높을 경우는 제1단계로서 교반기 회전수를 내리며, 교반기회전수가 설정하한치로 되었을 경우는 제2단계로서 통기가스중의 산소분압을 내리고, 통기가스중의 산소분압이 설정하한치로 되었을 경우는 제 3단계로서 통기량을 내리는 것을 특징으로하는 미생물 배양제어방법.
  8. 배양조의 압력측정수단, 배가스유량측정수단 및 배가스중의 탄산가스농도측정수단, 이들 각 수치에서, 배양조내의 탄산가스분압 및 미생물에 의한 탄산가스 생성량을 산출하며, 그것으로부터 균체량을 산출하는 수단, 그 수치로부터의 기질공급량의 결정수단, 상기 산출수단을 위한 수치설정수단 및 결정된 결과를 근거로 작동하는 기질공급량 조절수단의 조합으로 이루어진 것을 특징으로하는 미생물 배양제어장치.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019810004438A 1980-11-26 1981-11-17 미생물 배양제어방법 및 장치 KR870001649B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP165240 1980-11-26
JP16524080A JPS6018389B2 (ja) 1980-11-26 1980-11-26 微生物培養制御方法
JP80-165240 1980-11-26
JP56006332A JPS6018390B2 (ja) 1981-01-21 1981-01-21 微生物の好気的培養制御方法
JP81-6332 1981-01-21
JP2993181A JPS6015306B2 (ja) 1981-03-04 1981-03-04 微生物培養制御方法及びその装置
JP81-29931 1981-03-04

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KR830007820A true KR830007820A (ko) 1983-11-07
KR870001649B1 KR870001649B1 (ko) 1987-09-18

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US (1) US4444882A (ko)
EP (1) EP0052890B1 (ko)
KR (1) KR870001649B1 (ko)
DE (1) DE3176062D1 (ko)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100425779B1 (ko) * 2002-01-10 2004-04-01 설동근 가압식 세포 배양기
KR20160080543A (ko) * 2014-12-30 2016-07-08 (주)씨엔에스 세포 배양장치

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58187184A (ja) * 1982-04-23 1983-11-01 Hitachi Ltd 酸素富化ガスによる微生物の培養方法および装置
JPH078231B2 (ja) * 1985-03-25 1995-02-01 株式会社日立製作所 培養制御方法及び培養制御装置
US4720462A (en) * 1985-11-05 1988-01-19 Robert Rosenson Culture system for the culture of solid tissue masses and method of using the same
WO1988001643A1 (en) * 1986-08-29 1988-03-10 Endotronics, Inc. Method of culturing cells
CA2054860A1 (en) * 1990-11-09 1992-05-10 Hiroshi Kuriyama Process for continuously fermenting saccharides to produce alcohol using a flocculating microorganism
US5595905A (en) * 1992-03-12 1997-01-21 G.D. Searle & Co. Process control system for fed-batch fermentation using a computer to predict nutrient consumption
NL9402111A (nl) * 1994-12-13 1996-07-01 Inst Voor Agrotech Onderzoek Stelsel voor het regelen van de luchtsamenstelling binnen een bewaarruimte voor ademende plantaardige produkten.
US6280996B1 (en) 1999-03-22 2001-08-28 Praxair Technology, Inc. Method of using oxygen to eliminate carbon dioxide poisoning in aerobic fermentation
AU2001228322A1 (en) * 2000-01-28 2001-08-07 Alfa Laval Copenhagen A/S Method and apparatus for controlling growth of an organism in a nutrient medium
US7730761B2 (en) * 2006-06-16 2010-06-08 Rohm And Haas Company Producing stress on surfaces
EP1967545A1 (en) * 2007-03-09 2008-09-10 Rohm and Haas France SAS Cationic polymer latex
CN102399690B (zh) * 2011-09-18 2013-01-09 淮北市三和诺生物工程有限责任公司 高细胞密度发酵过程中溶氧控制系统
WO2017004236A1 (en) * 2015-06-29 2017-01-05 The Arizona Board Of Regents On Behalf Of The University Of Arizona Optical device for in-line and real-time monitoring of microorganisms
JP6138390B1 (ja) * 2017-01-25 2017-05-31 三菱化学エンジニアリング株式会社 マイクロナノバブルを用いた生物反応装置およびこの生物反応装置を用いた生物反応方法
WO2018206746A1 (en) * 2017-05-10 2018-11-15 Luxcel Biosciences Limited Real-time cellular or pericellular microenvironmental oxygen control
WO2020152017A1 (de) 2019-01-26 2020-07-30 Kazda Marian Verfahren zur automatischen überwachung von biogasreaktoren
DE102020103333B3 (de) * 2020-02-10 2021-05-06 Krinner Drucklufttechnik Gmbh Verfahren zum Vermehren von Hefezellen
CN112495273B (zh) * 2020-11-09 2023-01-10 内蒙古正建建设工程有限公司 一种建筑用水泥制备机

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE739021C (de) * 1937-11-17 1943-09-24 Max Seidel Verfahren und Einrichtung zum Regeln und UEberwachen des Prozessverlaufes bei der biologischen Verarbeitung von Naehrloesungen zum Zuechten von Mikroorganismen
DE2128744C3 (de) * 1971-06-09 1979-03-29 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V., 3400 Goettingen Verfahren zur Massenkultivation von Zellen und Geweben
DE2308087A1 (de) * 1973-02-19 1974-08-22 Kellner Maximilian Dipl Braur Fermentationsverfahren
US3887436A (en) * 1973-05-31 1975-06-03 Instrumentation Labor Inc Cell culturing system
CH618736A5 (en) * 1974-12-03 1980-08-15 Sick Peter Continuous process for the cultivation of microorganisms and apparatus for carrying it out.
US4064015A (en) * 1975-05-12 1977-12-20 New Brunswick Scientific Co., Inc. Control of biosynthesis in submerged culture
SU775122A1 (ru) * 1978-07-18 1980-10-30 Киевский Институт Автоматики Им. Хху Съезда Кпсс Способ автоматического управлени процессом культивировани микроорганизмов

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100425779B1 (ko) * 2002-01-10 2004-04-01 설동근 가압식 세포 배양기
KR20160080543A (ko) * 2014-12-30 2016-07-08 (주)씨엔에스 세포 배양장치

Also Published As

Publication number Publication date
EP0052890A3 (en) 1983-04-20
EP0052890A2 (en) 1982-06-02
DE3176062D1 (en) 1987-05-07
KR870001649B1 (ko) 1987-09-18
US4444882A (en) 1984-04-24
EP0052890B1 (en) 1987-04-01

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