KR20220106535A - Breeding method of korean native cattle using feed additive enhanced omega-3 fatty acid - Google Patents

Abstract

The present invention relates to a Korean cow feeding method using omega-3 fatty acid enriched feed additives, and more specifically, to a Korean cow feeding method using omega-3 fatty acid enriched feed additives, comprising the steps of: 1) separating lactic acid bacteria from a mixture of fermented soybeans and dried radish leaves based on enzyme activity and antibacterial activity; 2) preparing a feed additive by crushing alfalfa, soft wheat, ryegrass, linseed and dried radish leaves; 3) preparing a feed by inoculating and fermenting the lactic acid bacteria separated in step 1) to the feed additive prepared in step 2); and 4) supplementarily using the feed prepared in step 4) periodically while allowing the Korean cattle to naturally graze and consume grass. The fatty acid-enhanced feed additive, according to the present invention, has a high omega-3 fatty acid content to omega-6, and shows the effects of moisture control function, mineral component enhancement, palatability increase, feed intake increase, antibiotic use reduction, feed efficiency increase and meat quality improvement effect for Korean cows. Because Korean cows can have fast growth and excellent immunity, maintaining good health, so there is no need for emergency measures in case of disease. Also, the meat quality of Korean beef is very tender and excellent.

Description

The method of breeding Korean cattle using an omega-3 fatty acid-enhanced feed additive {Breeding method of korean native cattle using feed additive enhanced omega-3 fatty acid}

The present invention relates to a method for feeding Korean cattle using an omega-3 fatty acid-enhanced feed additive.

Hanwoo is the only livestock native to Korea that remains as a native species among domestic cattle. In the past, such Korean cattle were able to be bred only with by-products such as grain feed together with rice husks and rice straw, but the cattle's constitution was acidified, causing frequent diseases during the breeding process, as well as poor quality of meat or meat quality. there has been

In particular, as domestic Korean beef farmers are increasingly dependent on imported grain feed, there are frequent cases of bankruptcy due to feed fluctuations and cattle price fluctuations. .

Moreover, it is a known fact that Korean beef livestock, which is not competitive in terms of production cost, is facing a major crisis like other local agricultural products as it is shaken from the ground up as imported meat can be sold at Hanwoo meat stores as the agricultural and livestock products are being fully opened.

If we look specifically at the reasons for this decline in international competitiveness, first, if the number of Korean cattle raised in the process of raising Korean cattle by domestic livestock farmers is high, disease infections naturally occur frequently, which increases the number of doses of preventive vaccines and antibiotics, which increases the burden on farmers. The number one factor is the increase in labor costs for disease detection and treatment of Korean beef as well as the increase.

In addition, there is a lack of awareness about feed in the process of raising Korean cattle, which is the second factor that the meat quality of Korean cattle deteriorates by feeding general hay or grain feed without selecting and administering high-nutrition and high-quality feed.

And now, as Korean cattle breeding is commercialized and large-scale, it is difficult to supply feed with only by-products from farms, so feed and feed additives have been developed and supplied in large quantities. However, it is expected that domestic cattle breeding will not only be avoided, but the current status of Korean cattle and the number of herds will decrease exponentially.

Therefore, our priority is to secure international competitiveness and differentiation of beef production through the advancement of quality of Korean beef as well as stabilization of the supply of Korean beef by reducing production costs and improving meat quality through a scientific approach to breeding and breeding management. It is a task.

Since ruminants such as Hanwoo have physiological characteristics, the availability of feed varies greatly depending on the feed feeding method, so it is possible to improve the meat quality of Korean beef by changing the feed feeding method. is a trend

However, conventional TMR feed alone cannot maximize feed efficiency, and there is a risk of lactic acid poisoning, fourth gastric dislocation, keratosis and acidosis, etc. In severe cases, feed intake may be stopped, so TMR feeds with buffers have been introduced.

The buffers currently distributed in the market are expensive and have a problem of increasing the burden on farms. In Korea, inventions related to forage such as Korean Patent Registration No. 10-0385930 (auxiliary feed composition for ruminant livestock and feeding method thereof) are introduced. became It is composed of 100 parts by weight of a mixture consisting of 20 to 80% by weight of persimmon skin and 20 to 80% by weight of malt and 0.5 to 7 parts by weight of activated carbon. However, there is a limit to improving the meat quality only with the roughage as described above, and there is a need for improvement of the TMR feed providing major nutrients.

On the other hand, the fat of Korean beef is a saturated fatty acid, which solidifies in the human body and interferes with blood flow and raises the cholesterol level, which is known to cause adult diseases. In Korean Patent No. 10-0347907 (Korean beef breeding method), brown rice fermented vinegar is mixed in a ratio of 100:1 to 150:1 with respect to water and used as drinking water. 100 parts by weight to 1400 parts by weight, 300 parts by weight to 400 parts by weight of wheat bran, 9 parts by weight to 11 parts by weight of sesame cake, 85 parts by weight to 120 parts by weight of brown rice bran, 20 parts by weight to 30 parts by weight of refined loess soil, and then mixing , The mixture is fermented in a fermentation tank for about 1 week, and 1 to 2 parts by weight of a mixture of bamboo salt and bamboo charcoal is mixed and used. The prior invention has excellent immunity to diseases and can obtain fast growth and excellent meat quality in breeding Korean beef, but it does not control the supply according to the growth period of Korean beef, and in the process of fermenting brown rice, licorice, Angelica, Cinnamon, garlic, ginger, and jujube are mixed with liquefied processing and calcium is added, so it takes too much cost to raise Korean beef, and cholesterol levels in the human body increase when Korean beef is consumed.

U.S. Patent No. 5250307 discloses a method of emulsifying fatty acid calcium to provide a feed additive with a high content of unsaturated fatty acids that maintains a liquid state even at room temperature. A, minerals, etc. are added, emulsified, dried, and then powdered and commercialized. However, the above method uses a large amount of water to accelerate the deterioration of fat due to the difficulty of drying and the high moisture content of 3 to 5% even after rancidity and drying of the lipids. In addition, since the fat content is 50%, and calories are low, there is a problem that the fattening effect is small in a short breeding period.

Feed additives for animal growth promotion and disease control, feed efficiency improvement, and product quality improvement have contributed to inhibiting the growth of pathogens and improving the productivity of livestock products. As various problems have been raised that ingested antibiotics cause resistance in the human body, research on new types of non-antibiotic feed additives such as probiotics, enzymes, yeast, and natural product additives is being actively conducted.

As part of these studies, feed or feed additives are being developed to increase the content of omega-3 fatty acids in the meat of domesticated animals.

Omega-3 fatty acids are synthesized in a very small amount in the human body, and the metabolic rate is also very fast, so there is only a very small amount in the human body. Therefore, it is essential to consume foods rich in omega-3 fatty acids. In this situation, various studies are being made on food to supply omega-3 fatty acids. Although the research results using fish oil, fish meal, perilla, rapeseed, evening primrose, etc. are known, representatively, omega-3 using other raw materials Fatty acid feed is also known to have insignificant levels of fatty acids in meat.

The reality is that livestock that have been fed corn-based feed rich in omega-6 in factory-style barns are slaughtered and brought to the household table. The beef that comes to the table today is a result of grain (corn)-oriented feed and factory-style livestock breeding, which causes adult diseases of modern people due to fatty acid imbalance. is being occupied Therefore, it can be said that the development of beef capable of providing a balanced fatty acid supply and the development of a new livestock feed with an increased content of omega-3 fatty acids are urgently needed.

Accordingly, the present inventors raised cattle in a European-style barn of a wide space rather than a factory-style barn, and tried to produce Korean beef that was good for the body and tasted good by breeding cows with grass-based feed rather than corn-based feed. We developed a method of breeding Korean cattle using feed additives with an increased content of medium and omega-3 fatty acids. Specifically, we manufactured feed additives such as alfalfa, soft wheat, ryegrass, flaxseed, vitamin E and grass vinegar in addition to natural grass for Korean cattle. Here, the present invention was completed by confirming that a large amount of omega-3 fatty acids could be ingested and accumulated in Korean beef by using a feed fermented using lactic acid bacteria isolated from a mixed solution of fermented soybeans and fermented leaves.

Korean Patent Publication No. 10-0347907 (Registration Date: 2002.07.25.) US Registered Patent Publication No. 5250307 (Registration Date: October 5, 1993)

The purpose of the present invention is to feed grass-based feed so that the ratio of omega-3 to omega-6 is 1:1 to 4, so that the growth of Korean beef is fast and it has excellent immunity, so it is not necessary to take emergency measures according to disease. It is not only necessary, but in particular, to provide a method of breeding Korean beef so that the meat quality of the Korean beef is very soft and excellent.

In order to achieve the above object, the present invention comprises the steps of: 1) isolating lactic acid bacteria from a mixture of fermented soybeans and radish leaves based on enzyme activity and antibacterial activity; 2) preparing a feed additive by crushing alfalfa, soft mackerel, ryegrass, flaxseed and leaves of Shiraegi; 3) preparing a feed by inoculating and fermenting the feed additive prepared in step 2) with the lactic acid bacteria isolated in step 1); And 4) naturally grazing Korean beef while ingesting grass, periodically using the feed prepared in step 4) as an auxiliary; provides a method of feeding Korean beef using an omega-3 fatty acid-enhanced feed additive comprising a.

Hereinafter, the present invention will be described in detail.

In the method of breeding Korean cattle using the omega-3 fatty acid-enhanced feed additive of the present invention, the lactic acid bacteria isolated in step 1) have high glucose availability and high dependence on yeast extract, and high amylase, CMCase, and protease enzyme activities. It is preferable to be selected.

In addition, in the method of feeding Korean cattle using the omega-3 fatty acid-enhanced feed additive of the present invention, it is preferable to additionally mix vitamin E and wood vinegar with the feed additive of step 2), and in this case, the feed additive is alfalfa 20 parts by weight, 20 parts by weight of soft wheat, 20 parts by weight of ryegrass, 20 parts by weight of flaxseed, and 20 parts by weight of flaxseed leaves, more preferably, 10 ^-7 to 10 ^-3 parts by weight of vitamins, and, in addition, 2) For the feed additive in the step, acorn powder (Quercus acutissima Carr), mung bean powder (Vigna radiata L. Wilcze) and grape vine leaf powder extract in the same amount were put into the extractor, respectively, and heated at 100°C. It is most preferable to additionally include 20 parts by weight of a concentrated extract prepared by extraction and filtration at a temperature for 10 hours, and concentration to 6% solids.

In addition, in the method for feeding Korean cattle using the omega-3 fatty acid-enhanced feed additive of the present invention, the feed additive preferably has a ratio of omega-6/omega-3 fatty acid in a weight ratio of 1 to 4.

Ideally, the glue can be cut to a thickness of 2-3 mm and used as is. Alfalfa is a vitamin A complex that helps the normal functioning of the lungs and heart, lowers cholesterol levels in arteries, and prevents anemia due to its rich iron content. Oats have anticancer effects and contain abundant proteases to suppress free radicals, reduce body fat and prevent diabetes. In addition, beta-glucan component prevents fat accumulation in the body and inhibits carbohydrate absorption. The alfalfa, oats and ryegrass are fibrous, and are added to improve the digestive function of Korean beef, facilitate rumination, and compensate for nutritional deficiencies caused by grains. These fibers are rich in vitamins and minerals, which promotes the growth and development of Korean beef, increases resistance to disease, and aids in protein digestion. Avoid Korean beef by using feeds that use additives that can increase the ratio of alfalfa, soft wheat, ryegrass and omega-3.

The ratio of omega-3 to omega-6 is 1:100 in the meat of Korean beef fed with general feed, resulting in an imbalance of fatty acids. This imbalance causes people to develop inflammatory diseases, including heart disease, diabetes, and cancer. Therefore, the present inventors prevented fatty acid imbalance due to meat intake by feeding flaxseed feed with an omega-3 content 44 times that of mackerel to breeding cattle.

When we say omega-3, we usually refer to omega-3 fatty acids. A double bond is formed from the third carbon in the carbon chain constituting the fatty acid molecule The unsaturated fatty acids formed are called omega-3 fatty acids. Omega-3, a type of fatty acid, is an essential fatty acid and is a drug used to treat dyslipidemia or to supply fat. Omega-3 used as a drug includes omega-3-acid triglycerides in the general form and omega-3-acid ethyl ester90 purified using ethanol. have. Omega-3-acid ethyl ester 90, in the form of an oral drug, reduces triglyceride synthesis in the liver and, together with other drugs, prevents secondary occurrence after myocardial infarction. Omega-3-acid triglycerides are in the form of injections and provide fat to patients who need nutrition along with other drugs. Omega-3 is commonly used as a health functional food in addition to pharmaceuticals. In this case, it is used for the purpose of improving blood triglyceride, improving blood circulation, improving memory, or improving eye health by improving dry eyes.

Fatty acid-enhanced feed additive according to the present invention has a high omega-3 fatty acid content for omega-6, moisture control function in Korean beef, mineral ingredient reinforcement, palatability, increase feed intake, reduction in use of antibiotics, increase in feed efficiency and improvement of meat quality As it shows the effect, Korean beef has fast growth power and excellent immunity, so it does not need emergency measures according to disease, and especially, the meat quality of Korean beef is very soft and excellent.

Hereinafter, preferred embodiments according to the present invention will be described in detail by presenting more specifically. However, the following examples are provided so that those of ordinary skill in the art can fully understand the present invention, and can be modified in various other forms, and the present invention is limited by the above examples. it is not

<Example 1> Selection of fermented strains for breeding Korean beef

The present inventors used a mixed solution of fermented soybeans and radish leaves to isolate lactic acid bacteria for raising Korean beef. The fermented leaves were pulverized, and the lactic acid bacteria culture medium, MRS (Difco ^™ , USA) broth was added, and the fermented broth was used as a separation source. Specifically, the fermented soybean suspension and the naturally fermented mixed fermented soybean leaf were homogenized in a sterilized physiological saline (0.8% NaCl solution) solution, and the suspension was sequentially diluted and each LB (Difco ^™ , USA) agar plate (agar plate). ), PD (Difco ^™ , USA) agar plate, and MRS agar plate. The plate coated with the suspension dilution was incubated at 30° C. for 24 hours, and colonies formed morphologically different from each other were separated and used for the experiment by visually observing the formed colonies.

As a result, the isolated strain was selected based on the results of analysis based on enzyme activity, pH of the culture medium, and antibacterial activity. Each specific method is as follows.

<1-1> Digestive enzyme activity analysis

The secretion of each enzyme was evaluated using a medium capable of determining the presence or absence of protein, fibrin, and starch degrading enzymes in the isolated microorganism.

Protease decomposition ability is based on NA (nutrient agar), and after spreading each strain on a medium prepared by adding 2% skim milk, insoluble skim milk block is decomposed by culturing at 30°C for 24 hours. The formed growth inhibition range (clear zone) was observed. Cellulase is plated with each strain on a medium in which 1% CMC (Carboxymethyl cellulose) is added to NA, and then cultured at 30° C. for 24 hours. CMC remaining in the medium is treated with 0.2% Congo red solution. By staining, the growth inhibition range formed due to the degradation of CMC was observed. Finally, amylase was inoculated with each strain in a medium produced by adding 1% soluble starch to NA and cultured at 30°C for 24 hours, followed by 0.2% iodine/0.2% potassium iodine. By staining non-decomposed starch with a solution, the growth inhibition range formed through starch decomposition was observed.

Direct-fed microbials (DFMs) that secrete digestive enzymes have the effect of improving the efficiency of nutrient utilization by showing the characteristics of improving the digestibility of feed. In particular, in ruminants, it is possible to improve the rumen fermentation environment by making various secondary metabolites that can be used by ruminant microorganisms.

Accordingly, all strains isolated from fermented soybeans through the present invention were evaluated to have very good activity as DFM capable of supplying digestive enzymes to livestock, and lactic acid bacteria isolated from the fermented fermented soybean leaves did not show the activity of digestive enzymes. Most of Lactic acid bacteria exhibit a fairly homotropic effect on the substrate, and similar results were also obtained in the present invention.

<1-2> Antibacterial activity analysis

The culture medium of the candidate strains was centrifuged (12,000 rpm, 10 min) to take the supernatant and used for evaluation of antibacterial activity. Antimicrobial activity was evaluated by agar well diffusion method. Using a sterile Pasteur pipette connected to a vacuum device prepared by the medium layer method, about 10 to 20 μl of wells were created on the prepared plate, and 30 μl of each culture supernatant prepared in each well was added. The medium to which the culture supernatant was added was selected as growth inhibitory rings produced after culturing for about 20 hours in a 37°C incubator.

After confirming a single colony on the LB anti-platelet plate, the test bacteria for the evaluation of antibacterial activity were cultured in LB broth for about 16 to 20 hours, and after addition of DMSO solution, -70°C deep freezer (MDF-U53V) , SANYO Electric Co., Ltd, Japan).

Probiotics are microorganisms that live in the gastrointestinal tract and have beneficial effects on humans or animals, and lactic acid bacteria are representative. Lactobacillus is a microorganism that has been widely used in the dairy industry that produces dairy products. It has various probiotic effects such as maintaining the balance of the intestinal flora, inhibiting the growth of harmful bacteria, preventing diarrhea, protecting intestinal epithelial cells, inhibiting absorption of toxic substances, and inhibiting carcinogenesis. is known to represent In order for lactic acid bacteria to act as probiotics, it must be resistant to gastric and bile acids, so it must be able to inhabit the gastrointestinal tract, and it must have antagonistic power against harmful bacteria as it settles and proliferates in the epithelial cells of the gastrointestinal tract. Therefore, according to the present invention, the isolated strain is considered to be able to give a high effect in preventing digestive diseases when feeding mixed livestock with fermented soybeans and fermented soybeans with strong antibacterial activity against pathogenic bacteria of the digestive tract that cause digestive diseases.

<1-3> Medium components for mass production of lactic acid bacteria

The selected strains were inoculated at 1% in runs in which various medium components were mixed and cultured at 30°C for 24 hours, and then the number of viable cells in the culture was evaluated using a sequential dilution method.

The number of viable cells in each run in which the strain isolated in Example 1 was cultured varied from 1.0×10 ⁸ cfu/ml to 1.0×10 ¹⁰ cfu/ml, and the availability of glucose was very high, and as a nitrogen source, yeast extract dependence was significantly high.

In the present invention, in order to isolate useful strains for fermentation of fermented soybean leaves, antibacterial activity against livestock pathogenic bacteria and enzyme activity of amylase, CMCase, and protease were analyzed to discover useful microorganisms from fermented soybeans and fermented fermented leaves, and optimal culture conditions for the strain were analyzed. established.

<Example 2> Preparation 1 of fermented grain feed and breeding of Korean cattle

In order to prepare the feed additive composition according to the present invention that can increase the ratio of omega-3 fatty acids in the feed for Korean cattle, the present inventors first found that 20 parts by weight of alfalfa, 20 parts by weight of soft wheat, and 20 parts by weight of ryegrass. , 20 parts by weight of flaxseed and 20 parts by weight of leaves of Shiraegi were crushed, respectively. After mixing 200 g of the crushed material and 200 ppm of vitamin E as an antioxidant, a feed additive with increased omega-3 fatty acids was prepared by spray-drying wood vinegar to increase antibacterial and antioxidant properties. The feed additive was put into a fermentation tank, and the lactic acid bacteria strain isolated in Example 1 was inoculated at 1% (w/w), fermented at 35° C. for 1 week, and used as an injection, and roughage such as hay was appropriately blended here. .

In the course of breeding Korean cattle, about 300 Korean cattle aged 3 months to 4 years were naturally grazing and raised, and the feed of Example 1 was additionally supplied while breeding for 5 years.

It was confirmed that the reared Korean beef did not develop any diseases compared to general Korean beef during the breeding process, and in particular, the growth rate was very fast, and the meat quality was very soft. As a result, it was possible to reduce the cost of disease outbreaks, and it is judged that the meat quality is very good compared to other Korean beef, so that it will be able to gain an edge in international competitiveness.

<Example 3> Preparation of fermented grain feed 2

Acorn powder (Quercus acutissima Carr), mung beanpowder (Vigna radiata L. Wilcze) and grape vine leaf powder extract in the same amount were put in each extractor at 100°C for 10 hours. 20 parts by weight of the concentrated extract prepared by extraction and filtration and concentration to 6% solids was prepared, and 20 parts by weight of alfalfa of Example 1, 20 parts by weight of soft wheat, 20 parts by weight of ryegrass, 20 parts by weight of flaxseed A feed additive was prepared in the same manner as in Example 1, except that it was mixed with the crushed material of 20 parts by weight of leaves and leaves of Shiraegi. This was used as a feed for Korean cattle in the same manner as in Example 2.

<Comparative Example 1> Preparation of feed 1

A feed additive was prepared in the same manner as in the feed of Example 2, except for the feed additive lacking the flaxseed component, and it was bred while being supplied to 10 Korean cattle.

<Comparative Example 2> Preparation of feed 2

A feed additive was prepared in the same manner as in the feed of Example 2, except that the lactic acid bacteria produced in Example 1 were not inoculated, and the feed additives were prepared while feeding 10 Korean cattle.

<Experimental Example 1> Component Analysis of Meat Produced from Raised Korean Beef

Before shipment, 9 Korean cattle per treatment group were repeatedly and completely randomized, and for 40 days each, experimental livestock feed (1.5% of body weight) and roughage (3.5% of body weight, such as rice straw and hay) were fed daily. After that, sirloin (beef) (9 points per treatment) was collected by sacrificing cattle, and the n-6/n-3 ratio was analyzed and statistically processed (p<0.05). The results are shown in Table 1 below.

fatty acid Example 2 Example 3 Comparative Example 1 Comparative Example 2 Omega-6 2.84 2.64 3.24 3.17 Omega-3 0.76 1.18 0.47 0.65 Omega-6/Omega-3 3.73 2.23 6.89 4.88

As shown in Table 1 above, the ratio of omega-6/omega-3 in the livestock feed group according to the present invention was 2.23 to 3.73, which was found to be in the ideal range, and was significant when compared with the control group (CO) fed the general feed and the comparative example. It can be seen that it is significantly lowered. In particular, a new fact shown in the present invention was that when a feed containing 6 to 10% of the composition for feed addition according to the present invention in Korean cattle feed was prepared and supplied, the omega-6/omega-3 ratio could be significantly lowered. .

In another experiment, when the flaxseed content was increased, cows refused to eat feed at levels above 10%. Therefore, when the feed composition according to the present invention is applied to Korean cattle, 6-8% of flaxseed in the feed is added and fed for 40 days before shipment, so that new high-functionality Korean beef with a lower omega-6/omega-3 ratio can be produced. is fed

<Experimental Example 2> Daily weight gain and feed requirement

12 Korean cattle with an average age of 6.8±1.5 years old were divided into 4 groups of 3 each, and each group was tested for 6 months while providing Examples 2 and 3 and commercially available compound feed and rice straw as controls. At this time, feed was allowed to be freely ingested, and water was allowed to be consumed at all times.

At this time, the general ingredients (% dry matter) of the commercially available compounded feed were 12.07±0.20% moisture, 14.02±0.24% crude protein, 4.82±0.06% crude fat, 50.64±0.45% crude fiber, and 9.84±0.04% crude flour.

And the daily weight gain and feed requirement of each test group were calculated and shown in Table 2 below. The daily weight gain was obtained by measuring the body weight before the start of the test and before the end of the test, and the average weight gain for each treatment was divided by the number of test days. The feed requirement was obtained by dividing the total feed intake by the total weight gain. At this time, the feed intake was calculated from the total amount of feed from the start of the test to the end of the test, excluding the remaining amount before weight measurement at the end of the test. At this time, the starting weight of each group was 477.4±23.2kg, 481.6±24.2kg, 478.1±21.8kg, and 480.2±20.9kg, respectively, which were similar levels.

division Weight per day (kg) Feed demand rate (feed/gain) Example 2 0.71±0.05 11.6±0.43 Example 3 0.74±0.06 11.0±0.42 control 0.64±0.08 10.8±0.46

As shown in Table 2, in Examples 2 and 3 according to the present invention, it was confirmed that the daily gain was higher and the feed requirement was lower than that of the control group.

<Experimental Example 3> Cholesterol content and fatty acid content

12 Korean cattle with an average age of 6.8±1.5 years old were divided into 4 groups of 3 animals each, and each group was tested for 6 months while providing Examples 2 and 3 and commercially available compound feed and rice straw as controls. At this time, feed was allowed to be freely ingested, and water was allowed to be consumed at all times.

After the test type, 2 heads were shipped from each group, and 2 kg each of the sirloin between the 12th and 13th ribs was sampled from the slaughtered carcass, and the cholesterol content and fatty acid content were analyzed.

At this time, for the analysis of the cholesterol content, after adding a standard material (5α-cholestane) to 1 g of the sample, 5 mL of 50% KOH (aq) and 22 mL of ethanol were added, saponified at 23 ° C. for 6 hours, extracted repeatedly, and this was extracted by gas chromatography (GC, DS 6200, Donam Co, Korea). And the results are shown in Table 3 below.

Item Cholesterol, mg/100g Example 2 62.88 Example 3 61.18 control 74.94

As shown in Table 3, it was confirmed that Examples 2 and 3 according to the present invention had a lower cholesterol content than the control group.

And for the analysis of fatty acid content, 0.7 ml of 10N KOH in water and 6.3 ml of methanol were mixed with 1 g of the sample and placed in a constant temperature water bath having a water temperature of 55° C. and then heated. After heating for 1 hour and 30 minutes, shake vigorously once every 30 minutes, cool in cold water prepared in advance for 1 to 2 minutes, add 0.58 ml of 24N H ₂ SO ₄ in water, and then place in a constant temperature water bath at 55 ° C for 1 hour. It was heated for 30 minutes and again vigorously shaken once every 30 minutes. After heating, 3 ml of hexane was added after cooling in prepared cold water, and centrifugation was performed at 3,000 rpm for 5 minutes (HANIL, Combi-514R, KOR). After putting it in a vial using a Pasteur pipette, the fatty acid was analyzed using a gas chromatograph-flame ionization detector (Agilent, 7890 series, USA) under the following conditions. The injector is a split mode with a split ratio of 25:1, and the temperature is 250℃, and the detector is a flame ionization detector (FID), and the temperature is 250℃.

High-purity air, high-purity H ₂ , and high-purity He were used as carrier gas, and the flow rate was 40 mL/min for H ₂ and 400 mL/min for air. The column for analysis was DB-WAX (30 m × 0.25 um × 0.25 mm). And the results are shown in Table 4 below.

Item Example 2 Example 3 control Palmitic acid (g/100g)
(palmitic acid, C _16:0 ) 23.74 23.54 27.17 Stearic Acid (g/100g)
(stearic acid, C _18:0 ) 7.08 7.04 12.25 Oleic acid (g/100g)
(oleic acid, C _18:1 ) 48.54 48.67 45.59 Linoleic acid (g/100g)
(linoleic acid, C _16:2n6 ) 1.99 2.01 1.81 Saturated fatty acid ratio (%) 37.89 37.63 45.40 Unsaturated fatty acid ratio (%) 62.11 62.37 55.60

As shown in Table 4, it was confirmed that the contents of palmitic acid and stearic acid, which are saturated fatty acids, were lower in Examples 2 and 3 compared to the control group. On the other hand, it was confirmed that Examples 2 and 3 showed higher oleic acid and linoleic acid, which are unsaturated fatty acids, compared to the control. In addition, in Examples 2 and 3 of the present invention, it was confirmed that the ratio of unsaturated fatty acids was significantly higher than that of saturated fatty acids.

Above, the present invention has been described in detail with reference to preferred embodiments, but the present invention is not limited to the above embodiments, and various modifications are made by those skilled in the art within the scope of the technical spirit of the present invention. This is possible.

Claims (6)

1) isolating lactic acid bacteria from the mixed solution of fermented soybeans and radish leaves based on enzyme activity and antibacterial activity;
2) preparing a feed additive by crushing alfalfa, soft mackerel, ryegrass, flaxseed, and leaves of shiraegi;
3) preparing a feed by inoculating and fermenting the feed additive prepared in step 2) with the lactic acid bacteria isolated in step 1); and
4) Naturally grazing Korean beef while ingesting grass, periodically using the feed prepared in step 4) as auxiliary;
The omega-3 fatty acid-enhanced feed according to claim 1, wherein the lactic acid bacteria isolated in step 1) have high glucose availability and high dependence on yeast extract, and are selected based on high amylase, CMCase, and protease enzyme activity. A method of breeding Korean beef using additives.
The method according to claim 1, wherein vitamin E and wood vinegar are additionally mixed with the feed additive of step 2).
According to claim 1 or 3, wherein the feed additive is alfalfa (alfalfa) 20 parts by weight, soft wheat 20 parts by weight, ryegrass (ryegrass) 20 parts by weight, flaxseed 20 parts by weight, and 20 parts by weight of flaxseed leaves 20 parts by weight of vitamin 10 ^- Korean beef specification method using omega-3 fatty acid-enhanced feed additive, characterized in that it consists of ⁷ to 10 ^-3 parts by weight.
The method of claim 4, wherein the feed additive in step 2) is acorn powder (Quercus acutissima Carr), mung bean powder (Vigna radiata L. Wilcze) and grape vine leaf powder extract (grape vine leaf powder extract) in the same Korean beef specification using omega-3 fatty acid-enhanced feed additive, characterized in that it additionally comprises 20 parts by weight of a concentrated extract prepared by putting it in an extractor as a content, extracting and filtering at a temperature of 100° C. for 10 hours, and concentrating to a solid content of 6%. Way.
The method according to claim 1, wherein the feed additive has a ratio of omega-6/omega-3 fatty acids in a weight ratio of 1-4.