KR102548441B1 - Breeding method of korean native cattle using feed additive enhanced omega-3 fatty acid - Google Patents

Abstract

The present invention relates to a method for feeding Korean cattle using an omega-3 fatty acid-enhanced feed additive, and more particularly, 1) separating lactic acid bacteria from a mixture of fermented soybeans and radish leaves based on enzyme activity and antibacterial activity; 2) preparing a feed additive by crushing alfalfa, soft wheat, ryegrass, linseed and leaves of dried radish; 3) preparing feed by inoculating and fermenting the lactic acid bacteria separated in step 1) to the feed additive prepared in step 2); and 4) supplementally using the feed prepared in step 4) periodically while feeding the grass by naturally grazing Korean cattle. The fatty acid-enhanced feed additive according to the present invention has a high omega-3 fatty acid content relative to omega-6, improves moisture control function, strengthens inorganic components, increases palatability, increases feed intake, reduces use of antibiotics, increases feed efficiency, and improves meat quality in Korean beef. As the effect is shown, the growth of Korean beef is fast and the immunity is excellent, so it grows healthy, so there is no need for emergency measures for diseases, and the meat quality of Korean beef is very tender and excellent.

Description

Korean cattle feeding method using feed additive enhanced omega-3 fatty acid {Breeding method of korean native cattle using feed additive enhanced omega-3 fatty acid}

The present invention relates to a method for feeding Korean cattle using an omega-3 fatty acid enriched feed additive.

Hanwoo is the only Korean domestic animal that remains as a native breed. In the past, it was possible to raise Korean cattle with rice hulls, rice straw, etc., and by-products from farms such as grain feed. there has been

In particular, as domestic Korean beef farms increasingly depend on imported grain feed, there are frequent cases of bankruptcy due to feed fluctuations and fluctuations in cattle prices. Also, cows have various problems such as weakened intestines and poor digestion and absorption rate. .

Moreover, it is a well-known fact that as imported meat can be sold at Korean beef stores with the recent full opening of agricultural and livestock products, Korean beef livestock, which is less competitive in terms of production cost, is shaken from its foundation and is facing a major crisis like other indigenous agricultural products.

Looking at the reasons for such a drop in international competitiveness in detail, first of all, if there are a large number of Korean cattle raised in the process of raising Korean cattle, domestic livestock farms naturally have frequent disease infections, increasing the number of times of administering preventive vaccines and antibiotics, which increases the burden on farms. The first factor is the increase in labor costs for disease detection and treatment of Korean cattle.

In addition, there is a lack of awareness about feed in the process of raising Korean cattle. The second reason is that the meat quality of Korean cattle is reduced by feeding general hay or grain feed instead of selecting and administering nutritious and high-quality feed.

And now, as Korean cattle breeding is commercialized and large-scaled, it is difficult to supply feed only with by-products from farms, so feed and feed additives are developed and supplied in large quantities. However, it takes a lot of cost to raise Korean cattle, and due to the continuous increase in breeding costs and the opening of the import market, not only domestic livestock farmers avoid breeding Korean cattle, but also the status of Korean cattle breeding and the number of breeding heads are expected to decrease exponentially.

Therefore, it is our priority to secure international competitiveness and differentiation of beef production through the high quality of Korean beef along with the stabilization of the supply of Korean beef by reducing production costs and improving meat quality through a scientific approach to breeding and breeding management. It is a task.

Since ruminant animals such as Korean cattle have different feed availability depending on the feed feeding method due to their physiological characteristics, the meat quality of Korean cattle can be improved by changing the feed feeding method. trend

However, conventional TMR feed alone cannot maximize feed efficiency, and there is a risk of causing lactic acid poisoning, 4th place displacement, keratosis, and acidosis, and rumination is reduced, resulting in lower digestibility, digestive disorders, and reduced feed intake. However, in severe cases, feed intake may be stopped, so a TMR feed with buffer added was introduced.

Currently, the buffers on the market are expensive, so there is a problem of increasing the burden on farmers. In Korea, an invention related to forages such as Korean Patent Registration No. 10-0385930 (Supplemental feed composition for ruminant livestock and its feeding method) is introduced. It became. It is composed of 100 parts by weight of a mixture consisting of 20 to 80% by weight of persimmon skin and 20 to 80% by weight of wheat bran and 0.5 to 7 parts by weight of activated carbon. However, there is a limit to improving the meat quality only with the roughage, so there is a need for improvement of the TMR feed that provides major nutrients.

On the other hand, the fat of Korean beef is a saturated fatty acid, which is solidified in the human body to interfere with blood flow and raises cholesterol levels, which are known to cause adult diseases. In Korean Registered Patent No. 10-0347907 (Korean beef breeding method), fermented brown rice vinegar is mixed at a ratio of 100:1 to 150:1 to water and used as drinking water, and after boiling 100 parts by weight of beans as feed, draining the water, 100 to 1400 parts by weight, 300 to 400 parts by weight of wheat bran, 9 to 11 parts by weight of seed meal, 85 to 120 parts by weight of brown rice bran, and 20 to 30 parts by weight of refined ocher are added and mixed. , The mixture is fermented in a fermentation tank for about one week, and 1 to 2 parts by weight of a mixture of bamboo salt and bamboo charcoal is mixed and used. In the prior invention, in breeding Korean cattle, immunity to diseases is excellent, rapid growth and excellent meat quality can be obtained, but the amount of supply is not adjusted according to the growth period of Korean cattle, and in the process of fermenting brown rice, By liquefying and mixing cinnamon, garlic, ginger, and jujube, and adding calcium preparations, it takes too much cost to raise Korean cattle, and the cholesterol level in the human body still increases when eating Korean beef.

U.S. Patent No. 5250307 is a method of emulsifying fatty acid calcium to provide a feed additive with a high content of unsaturated fatty acids that maintains a liquid state even at room temperature. It describes a method of adding A, minerals, etc., emulsifying, drying, and then making a product into a powder. However, the above method uses a large amount of water to accelerate the deterioration of fat due to the difficulty of drying, lipid rancidity, and moisture as high as 3 to 5% even after drying. In addition, the fat content is 50% and the calories are low, so there is a problem in that the fattening effect is low in a short breeding period.

Feed additives for promoting animal growth and suppressing disease, improving feed efficiency, and improving product quality have contributed to inhibiting the growth of pathogens and improving productivity of livestock products. As various problems that ingested antibiotics cause resistance in the human body have been raised, studies on new types of non-antibiotic feed additives such as probiotics, enzymes, yeasts, and natural additives are being actively conducted.

As part of these studies, feed or feed additives are being developed to increase the content of omega-3 fatty acids in the meat of raised animals.

Omega-3 fatty acids are synthesized in a very small amount in the human body, and the metabolism rate is also very fast, and only a very small amount exists in the human body. Therefore, it is essential to consume foods rich in omega-3 fatty acids. In this situation, various studies on food for supplying omega-3 fatty acids are being conducted. Representatively, research results using fish oil, fish meal, perilla, rapeseed, evening primrose, etc. are known, but omega-3 using other raw materials Fatty acid feeding is also known to have an insignificant level of fatty acid content in meat.

It is a reality that livestock that ate corn-based feed, which is high in omega-6, are slaughtered in factory farms and brought to the table at home. Beef, which is currently on the table, is a result of grain (corn)-oriented feed and factory-style livestock breeding, which causes adult diseases of modern people due to fatty acid imbalance. are being occupied Therefore, it can be said that the development of beef capable of providing a balanced fatty acid supply and the development of a new livestock feed with an increased content of omega-3 fatty acids are urgently needed.

Accordingly, the inventors of the present invention tried to produce Korean beef that is good for the body and tastes good by breeding cows in a large European-style barn instead of a factory-style barn, and breeding cows with grass-based feed rather than corn-based feed. A method for raising Korean cattle using feed additives with increased content of omega-3 fatty acids was developed. Here, the present invention was completed by confirming that a large amount of omega-3 fatty acids could be ingested and accumulated in Korean cattle by using feed fermented using lactic acid bacteria isolated from a mixture of fermented soybeans and dried leaves.

Korean Registered Patent Publication No. 10-0347907 (registration date: 2002.07.25.) US Patent Registration No. 5250307 (registration date: 1993.10.05.)

The purpose of the present invention is to feed grass-based feed so that the ratio of omega-3 and omega-6 can be 1: 1 to 4, so that Korean cattle grow quickly and have excellent immunity, so that there is no need for emergency measures according to disease. Not only is it not necessary, but it is to provide a method of raising Korean beef so that the meat quality of Korean beef is very tender and excellent.

In order to achieve the above object, the present invention is 1) separating lactic acid bacteria from fermented soybeans and dried leaves mixed solution based on enzyme activity and antibacterial activity; 2) preparing a feed additive by crushing alfalfa, soft wheat, ryegrass, linseed and leaves of dried radish; 3) preparing feed by inoculating and fermenting the lactic acid bacteria separated in step 1) to the feed additive prepared in step 2); and 4) supplementally using the feed prepared in the above step 4) periodically while feeding the grass by natural grazing of the Korean cattle.

Hereinafter, the present invention will be described in detail.

In the Korean beef feeding method using the omega-3 fatty acid-enhanced feed additive of the present invention, the lactic acid bacteria isolated in step 1) have high glucose utilization and dependence on yeast extract, and high amylase, CMCase, and protease enzyme activities. It is desirable to be selected.

In addition, in the method for feeding Korean cattle using the omega-3 fatty acid-enhanced feed additive of the present invention, it is preferable to additionally mix vitamin E and wood vinegar with the feed additive in step 2), wherein the feed additive is alfalfa 20 parts by weight, 20 parts by weight of soft malt, 20 parts by weight of ryegrass, 20 parts by weight of linseed, and 20 parts by weight of leaves of Shiraegi, more preferably 10 ^-7 to 10 ^-3 parts by weight of vitamins, and also the above 2) The feed additives in the step are acorn powder (Quercus acutissima Carr), mung bean powder (mung beanpowder; Vigna radiata L. Wilcze) and grape vine leaf extract (grape vine leaf powder extract) in the same amount, respectively, and put them in an extractor at 100 ° C. It is most preferable to further include 20 parts by weight of a concentrated extract prepared by extracting and filtering for 10 hours at a temperature and concentrating to a solid content of 6%.

In addition, in the method for feeding Korean cattle using the omega-3 fatty acid-enhanced feed additive of the present invention, the feed additive preferably has a weight ratio of omega-6/omega-3 fatty acids of 1 to 4.

Ideally, the glue can be cut to a thickness of 2 to 3 mm and used as is. Alfalfa is a complex of vitamin A, which helps normal lung and heart functions, lowers arterial cholesterol levels, and prevents anemia with its rich iron content. Oats (yeonmaek) have anti-cancer effects and contain abundant proteases to suppress active oxygen, reduce body fat and prevent diabetes. In addition, the beta glucan component prevents fat accumulation in the body and suppresses carbohydrate absorption. The alfalfa, oats, and ryegrass are fibers, which are added to improve the digestive function of Korean cattle, facilitate rumination, and compensate for nutritional deficiencies caused by grains. These fibers are rich in vitamins and minerals, so they promote the growth and development of Korean beef, increase resistance to diseases, and help digest proteins. Feed Korean cattle using feeds using alfalfa, soft malt, ryegrass and additives that can increase the ratio of omega-3.

The ratio of omega-3 to omega-6 is 1:100 in the meat of Hanwoo cattle fed on general feed, resulting in an imbalance of fatty acids. This imbalance causes people to develop inflammatory diseases, including heart disease, diabetes and cancer. Therefore, the present inventors prevented fatty acid imbalance due to meat intake by feeding cattle with a feed supplemented with flaxseed having an omega-3 content 44 times that of mackerel.

When called omega-3, it usually refers to omega-3 fatty acids. The double bond is formed from the carbon at the third end of the carbon chain constituting the fatty acid molecule (the carbon that is farthest from the carboxyl group, and the name was determined by taking advantage of the meaning of the last letter in Greek, omega (ω)). The unsaturated fatty acids formed are called omega-3 fatty acids. Omega-3, a type of fatty acid, is an essential fatty acid, and is a drug used to treat dyslipidemia or to supply lipids. Omega-3 used as a drug consists of omega-3-acid triglycerides in the normal form and omega-3-acid ethyl ester 90 purified using ethanol. there is. Omega-3-acid ethyl ester 90, in the form of an oral drug, reduces triglyceride synthesis in the liver and, together with other drugs, prevents secondary occurrence after myocardial infarction. Omega-3-acid triglycerides are in the form of injections, along with other drugs, to provide fat to patients in need of nutrition. In addition to medicines, omega-3 is commonly used as a health functional food. In this case, it is used to help improve blood neutral lipids, improve blood circulation, improve memory, or improve dry eyes to help improve eye health.

The fatty acid-enhanced feed additive according to the present invention has a high omega-3 fatty acid content relative to omega-6, improves moisture control function, strengthens inorganic components, increases palatability, increases feed intake, reduces use of antibiotics, increases feed efficiency, and improves meat quality in Korean beef. As the effect is shown, the growth of Korean beef is fast and the immunity is excellent, so it grows healthy, so there is no need for emergency measures for diseases, and the meat quality of Korean beef is very tender and excellent.

Hereinafter, preferred embodiments according to the present invention will be described in detail by presenting them in more detail. However, the following examples are provided to those skilled in the art to sufficiently understand the present invention, and can be modified in various forms, and the present invention is limited by the above examples. It is not.

<Example 1> Selection of fermented strains for breeding Korean cattle

The present inventors used a mixture of fermented soybeans and radish leaves in order to isolate lactic acid bacteria for breeding Korean cattle. Siraegi foliage was pulverized, and after adding MRS (Difco ^™ , USA) broth, which is a culture medium for lactic acid bacteria, the fermentation broth was used as a separation source. Specifically, the fermented soybean suspension and the naturally fermented radish leaf mixed fermented broth were homogenized in a sterilized physiological saline (0.8% NaCl solution) solution, and the suspension was serially diluted to LB (Difco ^™ , USA) agar plate (Agar plate). ), PD (Difco ^™ , USA) agar plates and MRS agar plates were smeared. The plate on which the suspension diluent was smeared was incubated at 30° C. for 24 hours, and colonies formed were visually observed to separate morphologically different colonies from each other and used in the experiment.

As a result, the isolated strain was selected based on the results of analysis based on enzyme activity, pH of the culture medium, and antibacterial activity ability. Each specific method is as follows.

<1-1> Analysis of digestive enzyme activity

The separated microorganisms were evaluated for secretion of each enzyme using a medium capable of determining the presence or absence of protein, fiber and starch degrading enzymes.

The decomposition ability of the protease is determined by plating each strain on a medium prepared by adding 2% skim milk based on NA (Nutrient Agar) and incubating at 30 ° C for 24 hours to decompose insoluble skim milk blocks. The formed growth inhibition range (clear zone) was observed. For cellulase, each strain was smeared on a medium containing 1% CMC (Carboxymethyl cellulose) in NA, incubated at 30 ° C for 24 hours, and then CMC remaining in the medium was removed with 0.2% Congo red solution. By staining, the extent of growth inhibition formed due to the decomposition of CMC was observed. Finally, amylase was inoculated with each strain in a medium created by adding 1% soluble starch to NA, incubated at 30 ° C for 24 hours, and then 0.2% iodine / 0.2% potassium iodine Starch that was not degraded with the solution was stained to observe the range of growth inhibition formed through starch decomposition.

DFM (direct-fed microbials) that secrete digestive enzymes have the effect of improving the efficiency of nutrient utilization by exhibiting the characteristics of improving the digestibility of feed. In particular, in ruminants, it is possible to improve the rumen fermentation environment by making various secondary metabolites available to rumen microorganisms.

Therefore, all the strains isolated from fermented soybeans through the present invention were evaluated to have very good activity as DFM capable of supplying digestive enzymes to livestock, and the lactic acid bacteria isolated from fermented leaves and leaves of Siraegi did not show the activity of digestive enzymes. Lactic acid bacteria show quite homotropic effect characteristics with respect to the substrate, and similar results were shown in the present invention.

<1-2> Analysis of antibacterial activity

The culture solution of the candidate strains was centrifuged (12,000 rpm, 10 min), and the supernatant was taken and used for antibacterial activity evaluation. Antibacterial activity was evaluated using an agar well diffusion method. Using a sterile Pasteur pipette connected to a vacuum device prepared by the multilayer medium method, wells of about 10 to 20 μl were created on the prepared plate to be tested, and 30 μl of the culture supernatant prepared for each well was added to each well. The medium to which the culture supernatant was added was selected as a growth-deteriorating ring produced after culturing for about 20 hours in a 37° C. incubator.

Specimens for antibacterial activity evaluation were distributed, and after confirming a single colony on the LB antiperspirant plate, they were cultured in LB broth for about 16 to 20 hours, and after adding DMSO solution, -70 ° C deep freezer (MDF-U53V , SANYO Electric Co., Ltd, Japan).

Probiotics are microorganisms that inhabit the gastrointestinal tract and exhibit beneficial effects on humans or animals, and lactic acid bacteria are representative examples. Lactic acid bacteria are microorganisms that have been widely used in the dairy industry that produces dairy products, and have various probiotic effects such as maintaining the balance of intestinal flora, inhibiting the growth of harmful bacteria, preventing diarrhea, protecting intestinal epithelial cells, inhibiting absorption of toxic substances, and inhibiting carcinogenesis. It is known to represent In order for lactic acid bacteria to act as probiotics, they must be resistant to gastric acid and bile acids, so they can inhabit the gastrointestinal tract, and must have antagonistic power against harmful bacteria while colonizing and proliferating in the epithelial cells of the gastrointestinal tract. Therefore, according to the present invention, the isolated strain is considered to be highly effective in preventing digestive diseases when feeding livestock with a mixture of fermented soybeans and leaves of Siraegi due to its strong antibacterial activity against pathogenic bacteria that cause digestive diseases.

<1-3> Media components for mass production of lactic acid bacteria

The selected strain was inoculated at 1% in runs mixed with various medium components and cultured at 30 ° C for 24 hours, and then the number of viable cells in the culture medium was evaluated using a sequential dilution method.

The number of viable cells in each run in which the strain isolated in Example 1 was cultured varied from 1.0×10 ⁸ cfu/ml to 1.0×10 ¹⁰ cfu/ml, and the utilization of glucose was very high, and yeast extract was used as a nitrogen source. The dependence on was found to be significantly high.

In the present invention, in order to isolate useful strains for fermenting Sirae foliar leaves, antibacterial activity against livestock pathogenic bacteria and activities of amylase, CMCase, and protease enzymes were analyzed to discover useful microorganisms from fermented soybeans and sagebrush leaves. established.

<Example 2> Production of Fermented Grain Feed 1 and Korean Cattle Breeding

In order to prepare a feed additive composition according to the present invention capable of increasing the ratio of omega-3 fatty acids in feed for Korean cattle, the present inventors first added 20 parts by weight of alfalfa, 20 parts by weight of soft wheat, and 20 parts by weight of ryegrass. , 20 parts by weight of linseed and 20 parts by weight of leaves of Siraegi were all crushed, respectively. A feed additive with increased omega-3 fatty acids was prepared by mixing 200 g of the lysate with 200 ppm of vitamin E as an antioxidant and then spray-drying wood vinegar to increase antibacterial and antioxidant properties. The feed additive was put in a fermentation tank and the lactic acid bacteria strain isolated in Example 1 was inoculated at 1% (w/w) and fermented at 35 ° C for 1 week to be used as an injection feed, where a forage such as hay was appropriately mixed. .

In the process of breeding Korean cattle, about 300 Korean cattle aged 3 months to 4 years were raised by natural grazing, and were reared for 5 years while additionally supplying the feed of Example 1 above.

It was confirmed that the raised Korean cattle had no disease at all during the breeding process compared to normal Korean cattle, and in particular, the growth rate was very fast and the meat quality was very tender. As a result, it was possible to reduce the cost of disease occurrence, and it is judged that it will be able to gain an upper hand in international competitiveness because of its excellent meat quality compared to other Korean beef.

<Example 3> Production of fermented grain feed 2

Acorn powder (Quercus acutissima Carr), mung beanpowder (Vigna radiata L. Wilcze), and grape vine leaf extract (grape vine leaf powder extract) in equal amounts were put into an extractor, respectively, and kept at 100°C for 10 hours. 20 parts by weight of the concentrated extract prepared by extracting, filtering, and concentrating to a solid content of 6% was prepared, and 20 parts by weight of alfalfa, 20 parts by weight of soft malt, 20 parts by weight of ryegrass, and 20 parts by weight of flaxseed were prepared. A feed additive was prepared in the same manner as in Example 1, except that 20 parts by weight of the leaves and leaves of Siraegi were mixed with the crushed material. This was used as feed for Korean cattle in the same manner as in Example 2.

<Comparative Example 1> Production of feed 1

A feed additive was prepared in the same manner as in the feed of Example 2, except for the feed additive lacking the linseed component, and fed to 10 Korean cattle while breeding.

<Comparative Example 2> Production of feed 2

A feed additive was prepared in the same manner as in the feed of Example 2, except that the lactic acid bacteria produced in Example 1 was not inoculated, and it was fed to 10 Korean cattle and raised.

<Experimental Example 1> Component analysis of meat produced from domesticated cattle

Each treatment group was repeatedly completely randomized to 9 heads of Korean cattle before shipment, and feed (1.5% of body weight) and roughage (3.5% of body weight, such as rice straw and hay) were fed daily for 40 days. Then, by sacrificing the cows, the sirloin (beef) (9 points per treatment group) was collected, and the n-6/n-3 ratio was analyzed and statistically processed (p<0.05). The results are shown in Table 1 below.

fatty acid Example 2 Example 3 Comparative Example 1 Comparative Example 2 Omega-6 2.84 2.64 3.24 3.17 Omega-3 0.76 1.18 0.47 0.65 Omega-6/Omega-3 3.73 2.23 6.89 4.88

As shown in Table 1, the omega-6/omega-3 ratio in the livestock feed group according to the present invention was in the ideal range of 2.23 to 3.73, and was significant when compared to the control group (CO) and comparative example consuming general feed. It can be seen that the lower In particular, a new fact revealed in the present invention is that the omega-6/omega-3 ratio can be significantly lowered when a feed containing 6 to 10% of the feed additive composition according to the present invention is prepared and supplied in Korean cattle feed .

In another experiment, cows refused to eat feed at levels higher than 10% when the flaxseed content was increased. Therefore, when the feed composition according to the present invention is applied to Korean cattle, if 6-8% of flaxseed is added in the feed and fed for 40 days before shipment, it is expected that new highly functional Korean beef meat with a lowered omega-6/omega-3 ratio can be produced. It is foreseeable.

<Experimental Example 2> Daily weight gain and feed conversion rate

Twelve Korean cattle with an average age of 6.8 ± 1.5 years were divided into 4 groups of 3 animals, and each group was tested for 6 months while providing Example 2 and Example 3 and commercially available feed and rice straw as controls. At this time, feed was allowed to be freely consumed, and water was allowed to be consumed at all times.

At this time, the general ingredients (% dry matter) of the commercially available feed were 12.07 ± 0.20% moisture, 14.02 ± 0.24% crude protein, 4.82 ± 0.06% crude fat, 50.64 ± 0.45% crude fiber, and 9.84 ± 0.04% crude ash.

In addition, the daily weight gain and feed conversion rate of each test group were obtained and shown in Table 2 below. The daily weight gain was obtained by measuring the weight before the start of the test and before the end of the test to obtain the weight gain, and the average weight gain for each treatment was obtained by dividing the test days, and the feed conversion rate was obtained by dividing the total feed intake by the total weight gain. At this time, the feed intake was calculated by excluding the remaining amount before weight measurement at the end of the test from the total amount of feed provided from the start of the test to the end of the test. At this time, the starting weights for each group were 477.4±23.2kg, 481.6±24.2kg, 478.1±21.8kg, and 480.2±20.9kg, respectively, which were similar.

division Weight per day (kg) Feed conversion rate (feed/gain) Example 2 0.71±0.05 11.6±0.43 Example 3 0.74±0.06 11.0±0.42 control 0.64±0.08 10.8±0.46

As shown in Table 2, it was confirmed that Examples 2 and 3 according to the present invention had higher daily weight gain and lower feed conversion rate than the control group.

<Experimental Example 3> Cholesterol content and fatty acid content

Twelve Korean cattle with an average age of 6.8 ± 1.5 years were divided into 4 groups of 3 animals, and each group was tested for 6 months while providing Example 2 and Example 3 and commercially available feed and rice straw as controls. At this time, feed was allowed to be freely consumed, and water was allowed to be consumed at all times.

After the test, 2 heads of each group were shipped, and 2 kg of each sample was taken from the sirloin between the 12th and 13th ribs from the slaughtered carcass, and then the cholesterol content and fatty acid content were analyzed.

At this time, the analysis of the cholesterol content was performed by adding a standard substance (5α-cholestane) to 1 g of the sample, adding 5 mL of 50% KOH (aq) and 22 mL of ethanol, saponifying at 23 ° C. for 6 hours, repeatedly extracting, and performing gas chromatography. (GC, DS 6200, Donam Co, Korea). And the results are shown in Table 3 below.

item Cholesterol, mg/100g Example 2 62.88 Example 3 61.18 control 74.94

As shown in Table 3, it was confirmed that Examples 2 and 3 according to the present invention had a lower cholesterol content than the control.

In addition, for the analysis of the fatty acid content, 0.7 ml of 10N KOH in water and 6.3 ml of methanol were mixed with 1 g of the sample, and the water was placed in a constant temperature water bath at 55 ° C and heated. While heating for 1 hour and 30 minutes, shake vigorously once every 30 minutes, cool in cold water prepared in advance for 1 to 2 minutes, add 0.58ml of 24N H ₂ SO ₄ in water, and then incubate in a constant temperature water bath at 55℃ for 1 hour. It was heated for 30 minutes, again vigorously shaken once every 30 minutes. After the heating was completed, 3 ml of hexane was added after cooling in the prepared cold water and centrifuged at 3,000 rpm for 5 minutes (HANIL, Combi-514R, KOR). After putting it in a vial using a Pasteur pipette, fatty acid analysis was analyzed using a Gas chromatograph-flame ionization detector (Agilent, 7890 series, USA) under the following conditions. The injector was a split mode with a split ratio of 25:1 and the temperature was 250℃, and the detector was a flame ionization detector (FID) and the temperature was 250℃.

High-purity Air, high-purity H ₂ , and high-purity He were used as carrier gases, and the flow rate was 40 mL/min for H ₂ and 400 mL/min for air. A column for analysis was DB-WAX (30 m × 0.25 um × 0.25 mm). And the results are shown in Table 4 below.

item Example 2 Example 3 control Palmitic acid (g/100g)
(palmitic acid, C _16:0 ) 23.74 23.54 27.17 Stearic acid (g/100g)
(stearic acid, C _18:0 ) 7.08 7.04 12.25 Oleic acid (g/100g)
(oleic acid, C _18:1 ) 48.54 48.67 45.59 Linoleic Acid (g/100g)
(linoleic acid, C _16:2n6 ) 1.99 2.01 1.81 Saturated Fatty Acid Ratio (%) 37.89 37.63 45.40 Unsaturated Fatty Acid Ratio (%) 62.11 62.37 55.60

As shown in Table 4, it was confirmed that the contents of palmitic acid and stearic acid, which are saturated fatty acids, were lower in Examples 2 and 3 than in the control. On the other hand, it was confirmed that oleic acid and linoleic acid, which are unsaturated fatty acids, were higher in Examples 2 and 3 than in the control group. In addition, it was confirmed that in Examples 2 and 3 of the present invention, the ratio of unsaturated fatty acids was significantly higher than that of saturated fatty acids.

Although the present invention has been described in detail with preferred embodiments, the present invention is not limited to the above embodiments, and various modifications are made by those skilled in the art within the scope of the technical idea of the present invention. this is possible

Claims (6)

1) Separating lactic acid bacteria from a mixture of fermented soybeans and dried leaves based on enzyme activity and antibacterial activity;
2) preparing a feed additive by crushing alfalfa, soft wheat, ryegrass, linseed and leaves of dried radish;
3) preparing feed by inoculating and fermenting the lactic acid bacteria separated in step 1) to the feed additive prepared in step 2); and
4) A method for feeding Korean cattle using an omega-3 fatty acid-enhanced feed additive, comprising the step of periodically supplementally using the feed prepared in step 3) while allowing the cattle to naturally graze and consume grass.
The omega-3 fatty acid enriched feed according to claim 1, wherein the lactic acid bacteria isolated in step 1) are selected based on their high glucose availability, high dependence on yeast extract, and high amylase, CMCase, and protease enzyme activities. Korean beef breeding method using additives.
[Claim 2] The method for feeding Korean beef using an omega-3 fatty acid enriched feed additive according to claim 1, wherein the feed additive in step 2) is additionally mixed with vitamin E and wood vinegar.
The method of claim 1, wherein the feed additive in step 2) comprises 20 parts by weight of alfalfa, 20 parts by weight of soft wheat, 20 parts by weight of ryegrass, 20 parts by weight of linseed, and 20 parts by weight of leaves of dried radish, wherein Korean beef feeding method using an omega-3 fatty acid enriched feed additive further comprising vitamin E 10 ^-7 to 10 ^-3 parts by weight
The method of claim 4, wherein the feed additive in step 2) is acorn powder (Quercus acutissima Carr), mung bean powder (Vigna radiata L. Wilcze) and grape leaf extract (grape vine leaf powder extract). Hanwoo beef specification using an omega-3 fatty acid-enhanced feed additive, characterized in that it further includes 20 parts by weight of the concentrated extract prepared by putting each extractor into an extractor at a temperature of 100 ° C. for 10 hours, and concentrating to a solid content of 6%. method.
The method according to claim 1, wherein the feed additive has a weight ratio of omega-6/omega-3 fatty acids of 1 to 4.