KR102649857B1 - Feed additive composition to improve digestive function and method for manufacturing feed additive composition - Google Patents

Abstract

The present invention is a feed additive that improves intestinal health by reducing harmful microorganisms in the intestines of livestock, promotes improved digestive function in livestock by improving intestinal villi by increasing production of fatty acids such as organic acids, and increases feed intake by improving the palatability of livestock. It relates to compositions and methods for producing them.

Description

Feed additive composition for improving digestive function and method for manufacturing the same {FEED ADDITIVE COMPOSITION TO IMPROVE DIGESTIVE FUNCTION AND METHOD FOR MANUFACTURING FEED ADDITIVE COMPOSITION}

The present invention is a feed additive that improves intestinal health by reducing harmful microorganisms in the intestines of livestock, promotes improved digestive function in livestock by improving intestinal villi by increasing production of fatty acids such as organic acids, and increases feed intake by improving the palatability of livestock. It relates to compositions and methods for producing them.

Feed is food that provides nutrition to animals such as livestock or companion animals, maintains life, and is necessary for health maintenance or growth, and includes sweetened feed, compounded feed, and supplementary feed.

Sweet feed refers to raw feed that is made of vegetable, animal, or mineral substances and is used directly as feed or as a raw material for compound feed. At this time, good feed must have a high ability to supply nutrients to livestock, be harmless and non-toxic to livestock, have a high production volume and be easily available, the nutrients must be fresh and not easily deteriorated, and the digestibility of nutrients must be high.

Blended feed is a mixture or processing of sweet feed, auxiliary feed, etc. in an appropriate ratio, and can be manufactured by varying the ratio depending on the intended use. Meanwhile, supplementary feed is added to feed to prevent feed quality deterioration or increase feed utility, and includes essential minerals and vitamins.

In addition, functional feed additives that can contribute to improving and improving the health of livestock can be mixed and fed.

Improving the digestive function of livestock is the most useful method of improving profitability in the livestock industry. Since digestive function is easily affected by livestock immunity, reproduction, stress, etc., controlling factors that affect digestive function and improving the intestinal health of livestock at the same time It is important to improve.

Republic of Korea Patent No. 1247687 showed that P5 peptide was added to the feed of weaned piglets or broiler chickens as an antibiotic substitute, and the results showed that the group fed antibacterial peptide P5 suppressed the growth of harmful microorganisms in weaned piglets compared to the control group, and improved nutrient digestibility to feed the piglets. We are launching technology to improve performance.

In that the above technology is used as a replacement for antibiotics by adding it to the feed of pigs and chickens and improves the digestibility of nutrients, like the present invention, it is a feed additive for ruminant animals such as cattle that reaches the intestines through the rumen. As such, technology for improving intestinal health by improving the intestinal environment is not disclosed.

Republic of Korea Patent No. 2548441 is a Korean beef feeding method using an omega-3 fatty acid-enhanced feed additive, comprising: 1) isolating lactic acid bacteria based on enzyme activity and antibacterial activity from a mixture of fermented soybeans and Sirae foliage; 2) manufacturing a feed additive by crushing alfalfa, ryegrass, linseed, and thyme foliage; 3) inoculating the feed additive prepared in step 2) with the lactic acid bacteria isolated in step 1) and fermenting it to produce feed; and 4) allowing Korean cattle to naturally graze and consume grass while periodically using the feed prepared in step 4) as a supplement.

The above technology relates to a method of feeding Korean beef using omega-3 fatty acid-enhanced feed additives, and does not disclose technology for improving intestinal health by suppressing and killing harmful intestinal bacteria.

Therefore, there is a need to develop feed additives that improve intestinal health by suppressing and killing harmful intestinal bacteria, promote improved digestive function in livestock by improving intestinal villi, and increase feed intake by improving livestock palatability.

Republic of Korea Patent No. 1247687 (2013.03.20) Republic of Korea Patent No. 2548441 (2023.06.22)

The purpose of the present invention is to provide a feed additive composition and a method for manufacturing the same that can improve intestinal health by reducing harmful microorganisms in the intestines of livestock.

The purpose of the present invention is to provide a feed additive composition and a method for producing the same that promote the improvement of digestive function in livestock by improving intestinal villi by increasing the production of fatty acids such as organic acids.

Another object of the present invention is to provide a feed additive composition that increases feed intake by improving the palatability of livestock and a method for producing the same.

In order to solve the above problems, the feed additive composition according to the present invention contains 5 to 10% by weight of glucose oxidase, 8 to 12% by weight of bromelain, and 30 to 35% by weight of glucose. % and the remaining amount of puffed grains.

The glucose oxidase is an enzyme component that oxidizes glucose to gluconic acid to produce gluconic acid and hydrogen peroxide. The feed additive composition according to the present invention reacts with glucose in the intestine and undergoes an enzymatic reaction to produce hydrogen peroxide, killing salmonella, It has the effect of suppressing E. coli.

If it is added in excess of the above content, there is a problem that the difference in the sterilizing effect in the intestines compared to the amount added is not large, and if it is added in a smaller amount than the above content, it does not have a sufficient sterilizing effect in the intestines, reducing the effectiveness of the feed additive. There is a problem of deterioration.

Bromelain is a proteolytic enzyme (sulfhydryl protease) extracted from pineapple stems. It inhibits the action of proteins that cause various inflammatory diseases and promotes protein decomposition to break down fiber and impurities in the blood. It is a substance widely known to reduce inflammation and swelling and to be effective in relieving pain.

Bromelain can be used by mixing with puffed grains, and preferably, it can be pulverized while mixed with puffed grains so that bromelain and the components of the puffed grains are included in one particle.

In particular, compared to simply mixing and using, when pulverized at once to form a mixed powder within one particle, it can have the advantage of further increasing the feed preference of livestock.

In the present invention, the feed additive composition may further include 1 to 3% by weight of d-lauric acid.

Through this, the sterilizing effect in the intestines can be maximized. However, if it is added in excess of the above amount, there is a problem that the difference in effect compared to the amount added is minimal.

The present invention also provides a method for producing the feed additive composition, comprising the steps of (a) washing grains, drying them, and mixing them with a glucose solution;

(b) drying the grains that have undergone step (a) with cold air and then puffing them;

(c) uniformly mixing the grains that have undergone step (b) with bromelain powder and then pulverizing the mixture;

(d) mixing the pulverized product of step (c) with glucose oxidase and maintaining it at a temperature of 5 to 10° C. for 1 to 5 hours; and

(e) re-grinding the pulverized product that has passed step (d);

It provides a method for producing a feed additive composition comprising.

Step (a) is a step of washing grains and mixing them with a glucose solution, and prepares them so that they can be dissolved in distilled water in a state close to saturated solubility. Prepare to reach a saturated solubility level by minimizing the amount of distilled water compared to the amount of glucose.

Step (b) is a cold air drying step, and can be dried for 1 hour by applying air at a temperature of about 5°C at a speed of 1 to 2 m/s. In this process, in order for the glucose to be absorbed or remain on the surface of the grain, only the moisture is removed with cold air and then placed in a puffing machine to puff. Puffing conditions can be adjusted differently depending on the type of grain.

The step (c) is a step of mixing with bromelain. In this step, the puffed grains are pulverized and then mixed with bromelain powder, or the puffed grains are mixed with bromelain powder and then pulverized to powder. You can.

Then, in step (d), the mixture is mixed with glucose oxidase and maintained at a temperature of 5 to 10°C. In this step, the temperature is maintained at a low temperature to prevent the enzyme reaction from occurring in advance.

Step (e) is a step of re-grinding the pulverized product again, and is intended to mix the glucose oxidase and the previous pulverized product more uniformly.

Specifically, the grain may be one or more selected from the group consisting of white rice, brown rice, black rice, and fenugreek seeds.

More specifically, in step (e), di-lauric acid is added to the pulverized product after step (d), mixed while maintaining a temperature of 50 to 55°C, and mixed at a temperature of -10 to -5°C. After rapid cooling, it may be ground to 150 to 180 mesh.

By further including di-lauric acid as described above, it has the advantage of further improving the sterilizing effect in the intestine after ingestion by livestock.

The feed additive composition according to the present invention has the effect of improving intestinal health by reducing harmful microorganisms in the intestines of livestock.

The feed additive composition according to the present invention has the effect of promoting the improvement of digestive function in livestock by improving intestinal villi by increasing the production of fatty acids such as organic acids.

The feed additive composition according to the present invention has the effect of increasing feed intake by improving the palatability of livestock.

Hereinafter, each configuration of the present invention will be described in more detail so that those skilled in the art can easily implement it. However, this is only an example, and the scope of rights of the present invention is determined by the following contents. Not limited.

As used herein, “preferred” or “preferably” refers to an embodiment of the invention that has certain advantages under certain conditions. However, other embodiments may also be preferred under the same or different conditions. Additionally, the identification of one or more preferred embodiments does not mean that other embodiments are not useful, nor does it exclude other embodiments that are within the scope of the invention.

As used herein, the term “comprising” is used to list materials, compositions, devices, and methods useful in the present invention and is not limited to the listed examples.

In particular, in the present invention, the term "comprising" refers to various states, such as a state in which the component is included as is, a state in which the component is processed as a precursor, a state in which the component is satisfied after processing, etc. It is not limited to the examples listed.

Hereinafter, the present invention will be described in more detail based on examples, but this is only an exemplary description for understanding the present invention, and the scope of the present invention is not limited or restricted to the following examples.

<Example 1>

Fenugreek seeds sold commercially are washed, placed in glucose solution (distilled water), kept for 30 minutes, taken out, and dried for 1 hour by applying air at a temperature of about 5°C at a speed of 1 to 2 m/s.

Dried fenugreek seeds were placed in a puffing machine and puffed under the conditions of a drying temperature of 40°C, a moisture content of 10%, and a puffing temperature of 180°C. After sufficiently cooling, it was uniformly mixed with bromelain powder, and the mixture was pulverized into particles of 100 mesh. This ground product was mixed with glucose oxidase and placed in a constant temperature chamber maintained at a temperature of 35-40°C for 4 hours.

Transfer this to a glass container, add di-lauric acid, mix while maintaining a temperature of 50 to 55°C, rapidly cool to a temperature of -10 to -5°C, and grind to 150 mesh to prepare a feed additive composition. did.

In Example 1, glucose oxidase (30,000 iu/g product) 7% by weight, bromelain (60,000 CDU/g product) 10% by weight, glucose 33% by weight, di-lauric acid 1.5%. It was composed to include weight percentage and balance of fenugreek seeds.

<Example 2>

It was prepared in the same manner as Example 1, except that di-lauric acid was not added.

<Example 3>

It was prepared in the same manner as in Example 1, except that the puffed grains in Example 1 were not mixed with bromelain powder, but were ground into 100 mesh particles and then uniformly mixed with bromelain.

<Comparative Example 1>

It was prepared in the same manner as Example 1, except that black beans were used instead of fenugreek seeds.

<Comparative Example 2>

It was prepared in the same manner as Example 1, except that 5% by weight of bromelain was used.

<Comparative Example 3>

It was prepared in the same manner as Example 1, except that 15% by weight of bromelain was used.

<Comparative Example 4>

It was prepared in the same manner as Example 1, except that 3% by weight of glucose oxidase was used in Example 1.

<Comparative Example 5>

It was prepared in the same manner as Example 1, except that 13% by weight of glucose oxidase was used in Example 1.

[Experimental method]

This experiment was conducted on 20 beef cattle (weighing 620-630 kg) from Ranch A in Gangwon-do. All were prepared as females, and the feed additives of Examples and Comparative Examples were mixed with commercially available H feed at a ratio of 10:1.

1. Feed preference evaluation

The feed additives of each example and comparative example were mixed, and then the feed consumption rate was measured over a certain period of time. The daily feed consumption rate for 14 days was calculated for each individual, and the average value is shown below.

compound feed Weight (Kg) Daily feed consumption rate (%) Example 1 621 45 Example 2 622 39 Example 3 630 25 Comparative Example 1 629 19 Comparative example 2 626 12 Comparative Example 3 621 15 Comparative Example 4 622 17 Comparative Example 5 624 21

2. Evaluation of the effect of sterilizing harmful intestinal microorganisms

Examples and Comparative Examples To evaluate the sterilizing effect of the feed additive composition, the sterilizing effect was evaluated against E scherichia coli K88 and Salmonella typhimurium .

30 Holstein cattle (body weight 605 kg) were tested, and the test animals were fed commercial feed mixed with the feed additive of Example 1 twice a day (7:30 a.m., 3 p.m.) for 14 days.

Before the experiment, after feeding commercial feed without prior mixing of feed additives for 7 days, a quantitative test was conducted for the total bacterial count of E. coli (E scherichia coli ) K88 and Salmonella ( Salmonella typhimurium ) in feces, and the total bacteria count after feeding the feed for 14 days was performed. The rate of change in bacterial counts is shown in the table below.

Five horses were assigned to each experimental group, and the experimental results were expressed as the average value of the rate of change.

(1) Preparation of test solution

Add 200 mL of 0.1% peptone solution to 200 g of the sample from which the shells were removed, grind it, mix 20 mL of the grinding liquid with 80 mL of the same diluent, and use the final 10-fold dilution as the test solution. Use the same dilution without adding the sample as a control test solution to check whether the test operation is sterile.

(2) Estimation test

Inoculate 10 mL of the test solution according to the manufacturing method into five test tubes containing double-concentration MMGM medium, and inoculate 1 mL and 0.1 mL of the test solution into five test tubes containing MMGM medium, respectively, and incubate at 37 ± 1°C for 24 hours. Incubate ±2 hours. As a result of the culture, when the color of the medium in the test tube turns yellow, the presumptive test is considered positive and a confirmatory test is conducted.

(3) Confirmatory test

The MMGM test tube culture that was confirmed positive in the presumptive test is separated and cultured on BCIG agar medium (medium 73). After incubation at 44 ± 1°C for 24 ± 2 hours, if blue-green typical colonies are generated, it is determined to be positive for E. coli, and the number of E. coli in 100 g of sample is calculated according to the most probable number method.

Total bacterial count change rate (%) E. coli Salmonella Example 1 -21 -33 Comparative Example 1 -5 -2 Comparative example 2 +11 +15 Comparative Example 3 +4 +9 Comparative Example 4 -8 -2 Comparative Example 5 -4 0

Referring to Tables 1 and 2, it can be seen that the feed additive composition of the Example is superior to the feed additive composition of the Comparative Example in improving the preference of the feed.

In addition, it can be seen that the feed additive composition of Example 1 has an excellent sterilizing effect on intestinal bacteria compared to other comparative examples. The feed additive composition according to the present invention improves the preference of feed and has a sterilizing effect on intestinal harmful bacteria. It can be confirmed that it is excellent.

Claims (5)

(a) washing the grains, removing them and placing them in a glucose solution;
(b) removing the grains that have undergone step (a) above, drying them with cold air, and then puffing them;
(c) uniformly mixing the grains that have undergone step (b) with bromelain powder and then pulverizing the mixture;
(d) mixing the pulverized product of step (c) with glucose oxidase and maintaining it at a temperature of 5 to 10° C. for 1 to 5 hours; and
(e) re-grinding the pulverized product that has passed step (d);
As a method for producing a feed additive composition comprising,
The feed additive composition contains 5 to 10% by weight of glucose oxidase, 8 to 12% by weight of bromelain, 30 to 35% by weight of glucose, and the remaining amount of swelling, based on the total weight of the composition. A method for producing a feed additive composition comprising grain.
According to paragraph 1,
The feed additive composition is a method of producing a feed additive composition further comprising 1 to 3% by weight of di-lauric acid (d-lauric acid).
delete According to paragraph 1,
The grain in step (a) is a method of producing a feed additive composition wherein the grain is at least one selected from the group consisting of white rice, brown rice, black rice, and fenugreek seeds.
According to paragraph 1,
In step (e), di-lauric acid is added to the pulverized product that has gone through step (d), mixed while maintaining a temperature of 50 to 55°C, and rapidly cooled to a temperature of -10 to -5°C. , a method for producing a feed additive composition, which is ground to 150 to 180 mesh.