KR20210149734A - Rna-가이드된 엔도뉴클레아제를 사용한 dna 작제물의 통합을 위한 개선된 방법 - Google Patents

Rna-가이드된 엔도뉴클레아제를 사용한 dna 작제물의 통합을 위한 개선된 방법 Download PDF

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KR20210149734A
KR20210149734A KR1020217032629A KR20217032629A KR20210149734A KR 20210149734 A KR20210149734 A KR 20210149734A KR 1020217032629 A KR1020217032629 A KR 1020217032629A KR 20217032629 A KR20217032629 A KR 20217032629A KR 20210149734 A KR20210149734 A KR 20210149734A
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nucleotides
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donor dna
cells
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베이베이 딩
웬중 궈
옌량 장
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소렌토 쎄라퓨틱스, 인코포레이티드
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KR1020217032629A 2019-03-11 2020-03-11 Rna-가이드된 엔도뉴클레아제를 사용한 dna 작제물의 통합을 위한 개선된 방법 Withdrawn KR20210149734A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962816836P 2019-03-11 2019-03-11
US62/816,836 2019-03-11
US201962901735P 2019-09-17 2019-09-17
US62/901,735 2019-09-17
PCT/US2020/022056 WO2020185867A1 (en) 2019-03-11 2020-03-11 Improved process for integration of dna constructs using rna-guided endonucleases

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US (1) US20220145333A1 (https=)
EP (1) EP3938510A1 (https=)
JP (2) JP2022524435A (https=)
KR (1) KR20210149734A (https=)
CN (1) CN113825834A (https=)
AU (1) AU2020239050A1 (https=)
CA (1) CA3133226A1 (https=)
IL (1) IL286244A (https=)
MX (1) MX2021010938A (https=)
SG (1) SG11202109972QA (https=)
WO (1) WO2020185867A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4139345A1 (en) 2020-04-24 2023-03-01 Sorrento Therapeutics, Inc. Memory dimeric antigen receptors
WO2022015956A1 (en) 2020-07-15 2022-01-20 Sorrento Therapeutics, Inc. Improved process for dna integration using rna-guided endonucleases
WO2022061115A1 (en) * 2020-09-18 2022-03-24 Vor Biopharma Inc. Compositions and methods for cd7 modification
BR112023018948A2 (pt) * 2021-03-19 2023-10-17 Metagenomi Inc Edição multiplex com enzimas cas
JP2024513087A (ja) * 2021-04-07 2024-03-21 アストラゼネカ・アクチエボラーグ 部位特異的改変のための組成物及び方法
EP4370676A2 (en) * 2021-06-18 2024-05-22 Artisan Development Labs, Inc. Compositions and methods for targeting, editing or modifying human genes
WO2023097236A1 (en) * 2021-11-24 2023-06-01 The Rockefeller University Compositions and methods for generating immunoglobulin knock-in mice
CN115851591A (zh) * 2022-11-30 2023-03-28 山东博群生物技术有限公司 细胞因子刺激剂及诱导淋巴细胞产生细胞因子的方法
WO2026020171A2 (en) 2024-07-19 2026-01-22 Aera Therapeutics, Inc. Chimeric antigen receptor constructs

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5489677A (en) 1990-07-27 1996-02-06 Isis Pharmaceuticals, Inc. Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US6555674B2 (en) 2000-08-09 2003-04-29 Nsgene A/S JeT promoter
EP2281050B1 (en) * 2008-04-14 2014-04-02 Sangamo BioSciences, Inc. Linear donor constructs for targeted integration
EP3241902B1 (en) 2012-05-25 2018-02-28 The Regents of The University of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
AU2014361834B2 (en) 2013-12-12 2020-10-22 Massachusetts Institute Of Technology CRISPR-Cas systems and methods for altering expression of gene products, structural information and inducible modular Cas enzymes
WO2016057951A2 (en) * 2014-10-09 2016-04-14 Life Technologies Corporation Crispr oligonucleotides and gene editing
AU2016261358B2 (en) * 2015-05-11 2021-09-16 Editas Medicine, Inc. Optimized CRISPR/Cas9 systems and methods for gene editing in stem cells
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
AU2016338785B2 (en) * 2015-10-12 2022-07-14 E. I. Du Pont De Nemours And Company Protected DNA templates for gene modification and increased homologous recombination in cells and methods of use
CA3038960A1 (en) 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
WO2018175636A2 (en) * 2017-03-22 2018-09-27 Novartis Ag Compositions and methods for immunooncology
CA3067382A1 (en) * 2017-06-15 2018-12-20 The Regents Of The University Of California Targeted non-viral dna insertions
CA3071683A1 (en) * 2017-08-08 2019-02-14 Sangamo Therapeutics, Inc. Chimeric antigen receptor mediated cell targeting
SG11202008568WA (en) 2018-03-09 2020-10-29 Sorrento Therapeutics Inc Dimeric antigen receptors (dar)

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SG11202109972QA (en) 2021-10-28
EP3938510A1 (en) 2022-01-19
CN113825834A (zh) 2021-12-21
WO2020185867A1 (en) 2020-09-17
CA3133226A1 (en) 2020-09-17
JP2022524435A (ja) 2022-05-02
JP2024133347A (ja) 2024-10-01
AU2020239050A1 (en) 2021-11-04
MX2021010938A (es) 2022-01-06
US20220145333A1 (en) 2022-05-12
IL286244A (en) 2021-10-31

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P13-X000 Application amended

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PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

PC1203 Withdrawal of no request for examination

St.27 status event code: N-1-6-B10-B12-nap-PC1203

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

P22-X000 Classification modified

St.27 status event code: A-2-2-P10-P22-nap-X000

P22-X000 Classification modified

St.27 status event code: A-2-2-P10-P22-nap-X000