KR20190003939A - 유전자 조작된 미생물로부터 개선된 뮤콘산 생산 - Google Patents

유전자 조작된 미생물로부터 개선된 뮤콘산 생산 Download PDF

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KR20190003939A
KR20190003939A KR1020187028248A KR20187028248A KR20190003939A KR 20190003939 A KR20190003939 A KR 20190003939A KR 1020187028248 A KR1020187028248 A KR 1020187028248A KR 20187028248 A KR20187028248 A KR 20187028248A KR 20190003939 A KR20190003939 A KR 20190003939A
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gene
protein
genetically engineered
arog
muconic acid
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Korean (ko)
Inventor
라이언 실러스
미셸 스펜서
알. 로저스 요쿰
테론 헤르만
유다니 러셀
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피티티지씨 이노베이션 아메리카 코포레이션
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Application filed by 피티티지씨 이노베이션 아메리카 코포레이션 filed Critical 피티티지씨 이노베이션 아메리카 코포레이션
Priority to KR1020237044654A priority Critical patent/KR20240005196A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01025—Shikimate dehydrogenase (1.1.1.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11001—Catechol 1,2-dioxygenase (1.13.11.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01001—Transketolase (2.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01054—3-Deoxy-7-phosphoheptulonate synthase (2.5.1.54)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01002—Glucokinase (2.7.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01063—Protocatechuate decarboxylase (4.1.1.63)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01118—3-Dehydroshikimate dehydratase (4.2.1.118)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03004—3-Dehydroquinate synthase (4.2.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01001—Pyruvate carboxylase (6.4.1.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020187028248A 2016-03-02 2017-03-01 유전자 조작된 미생물로부터 개선된 뮤콘산 생산 Ceased KR20190003939A (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020237044654A KR20240005196A (ko) 2016-03-02 2017-03-01 유전자 조작된 미생물로부터 개선된 뮤콘산 생산

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662302558P 2016-03-02 2016-03-02
US62/302,558 2016-03-02
PCT/US2017/020263 WO2017151811A1 (en) 2016-03-02 2017-03-01 Improved muconic acid production from genetically engineered microorganisms

Related Child Applications (1)

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KR1020237044654A Division KR20240005196A (ko) 2016-03-02 2017-03-01 유전자 조작된 미생물로부터 개선된 뮤콘산 생산

Publications (1)

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KR20190003939A true KR20190003939A (ko) 2019-01-10

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ID=59744431

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KR1020187028248A Ceased KR20190003939A (ko) 2016-03-02 2017-03-01 유전자 조작된 미생물로부터 개선된 뮤콘산 생산
KR1020237044654A Ceased KR20240005196A (ko) 2016-03-02 2017-03-01 유전자 조작된 미생물로부터 개선된 뮤콘산 생산

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Country Status (8)

Country Link
US (2) US20200181658A1 (https=)
EP (1) EP3423563B1 (https=)
JP (1) JP7437116B2 (https=)
KR (2) KR20190003939A (https=)
CN (1) CN109415684B (https=)
ES (1) ES2947938T3 (https=)
SG (2) SG11201807544WA (https=)
WO (1) WO2017151811A1 (https=)

Cited By (1)

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WO2025193050A1 (ko) * 2024-03-14 2025-09-18 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 l-라이신의 생산 방법

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US11518975B2 (en) 2018-04-30 2022-12-06 Alliance For Sustainable Energy, Llc Engineered microorganisms for the production of intermediates and final products
CN118638832A (zh) * 2018-05-15 2024-09-13 杜克大学 用于对阿克曼菌进行基因操纵的系统和方法
WO2020080467A1 (ja) * 2018-10-17 2020-04-23 国立大学法人弘前大学 ムコン酸産生形質転換微生物及びその利用
CN112300972B (zh) * 2019-08-02 2023-06-30 南京理工大学 以木质素为原料生产粘糠酸的基因工程菌
CN112300973B (zh) * 2019-08-02 2022-06-24 南京理工大学 以苯丙氨酰-tRNA合成酶基因突变体为反向筛选标记的红球菌基因编辑方法
CN111004761A (zh) * 2019-12-02 2020-04-14 天津科技大学 一种l-酪氨酸基因工程菌及其生产l-酪氨酸的方法和应用
WO2021110993A1 (en) * 2019-12-04 2021-06-10 Synbionik Gmbh An efficient shuttle vector system for the expression of heterologous and homologous proteins for the genus zymomonas
CN112266892B (zh) * 2020-10-21 2021-10-08 中国科学院天津工业生物技术研究所 AroG的突变体及其在产氨基酸基因工程菌中的应用
CN112961878B (zh) * 2021-03-08 2023-04-25 昆明理工大学 一种植物乳杆菌的基因在叶酸生物生成中的应用
CN114540396A (zh) * 2022-02-24 2022-05-27 天津大学 希瓦氏菌株中葡萄糖代谢通路的构建方法
WO2025142411A1 (ja) * 2023-12-28 2025-07-03 株式会社Ｃｏ２資源化研究所 ｃｉｓ，ｃｉｓ－ムコン酸又はカテコールの生成能を有するヒドロゲノフィラス属細菌形質転換体

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WO2025193050A1 (ko) * 2024-03-14 2025-09-18 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 l-라이신의 생산 방법

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JP2019506886A (ja) 2019-03-14
CN109415684B (zh) 2022-06-07
EP3423563B1 (en) 2023-05-24
EP3423563A1 (en) 2019-01-09
WO2017151811A1 (en) 2017-09-08
EP3423563A4 (en) 2019-10-16
US20210277429A1 (en) 2021-09-09
KR20240005196A (ko) 2024-01-11
US11685938B2 (en) 2023-06-27
SG10202008244TA (en) 2020-10-29
US20200181658A1 (en) 2020-06-11
CN109415684A (zh) 2019-03-01
SG11201807544WA (en) 2018-09-27
JP7437116B2 (ja) 2024-02-22
ES2947938T3 (es) 2023-08-24

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