KR20180049000A - 세포 배양 - Google Patents

세포 배양 Download PDF

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KR20180049000A
KR20180049000A KR1020187009401A KR20187009401A KR20180049000A KR 20180049000 A KR20180049000 A KR 20180049000A KR 1020187009401 A KR1020187009401 A KR 1020187009401A KR 20187009401 A KR20187009401 A KR 20187009401A KR 20180049000 A KR20180049000 A KR 20180049000A
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South Korea
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medium
complete
dimensional
ali
williams
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English (en)
Korean (ko)
Inventor
다비드 보바르드
칼스타 뤼티히
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필립모리스 프로덕츠 에스.에이.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
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    • C12N2500/00—Specific components of cell culture medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/27—Lung cells, respiratory tract cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Sustainable Development (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020187009401A 2016-08-25 2017-08-17 세포 배양 Ceased KR20180049000A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16185725.5 2016-08-25
EP16185725.5A EP3287521B1 (en) 2016-08-25 2016-08-25 Cell culture
PCT/EP2017/070881 WO2018036910A1 (en) 2016-08-25 2017-08-17 Cell culture

Publications (1)

Publication Number Publication Date
KR20180049000A true KR20180049000A (ko) 2018-05-10

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Family Applications (1)

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KR1020187009401A Ceased KR20180049000A (ko) 2016-08-25 2017-08-17 세포 배양

Country Status (8)

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US (2) US11041145B2 (enExample)
EP (2) EP3940059A1 (enExample)
JP (2) JP6810742B2 (enExample)
KR (1) KR20180049000A (enExample)
CN (1) CN108138135A (enExample)
PL (1) PL3287521T3 (enExample)
RU (1) RU2018111219A (enExample)
WO (1) WO2018036910A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES3070913T3 (en) * 2018-05-16 2026-06-18 Inst Nat Sante Rech Med Method of culturing proliferative hepatocytes
EP3927417A4 (en) * 2019-02-19 2022-11-16 Iontox LLC METHOD FOR PREDICTING REPEATED-DOSE TOXICITY USING AN INTEGRATED ORGAN PLATFORM
CN113699202A (zh) * 2020-05-21 2021-11-26 华子昂 一种用混合细胞与人工细胞培养巢制备胶原蛋白的方法
WO2023282925A1 (en) * 2021-07-08 2023-01-12 Ourotech, Inc. Treatment efficacy prediction systems and methods
CN120005810A (zh) * 2025-04-18 2025-05-16 立沃生物科技（深圳）有限公司 肝祖细胞、应用及3d动态培养扩增方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2313377B (en) 1996-05-20 1998-07-01 Sumitomo Chemical Co Kit for diagnosis of hepatitis C and alcoholic cirrhosis
GB9913979D0 (en) 1999-06-17 1999-08-18 Univ Wales Medicine Spheroid preparation
US7351584B2 (en) 2002-02-26 2008-04-01 In Vitro Technologies, Inc. Hepatocyte bioreactor system for long term culture of functional hepatocyte spheroids
EP1496066A4 (en) 2002-03-25 2006-09-13 Japan Science & Tech Agency ANTIBODY RECOGNIZING PROLIFERATIVE HUMAN LIVER CELLS, PROLIFERATIVE HUMAN LIVER CELLS, AND FUNCTIONAL HUMAN LIVER CELLS
US7753192B2 (en) 2005-12-23 2010-07-13 The Coca-Cola Company Apparatus and method for orienting spheroidal containers and packaging beverages in spheroidal containers
JP5217220B2 (ja) 2007-04-12 2013-06-19 株式会社日立製作所 細胞分離装置
CA2693481A1 (en) * 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
EP2334804B1 (en) 2008-10-07 2013-12-04 True North Therapeutics, Inc. Co-culture compositions and methods
WO2010079602A1 (ja) 2009-01-08 2010-07-15 株式会社日立製作所 動物肝細胞の培養方法
GB201104511D0 (en) * 2011-03-17 2011-05-04 Univ Cardiff Cell based screening assay
JP5812816B2 (ja) * 2011-11-15 2015-11-17 コバレントマテリアル株式会社 細胞培養担体及びその製造方法
DE102011121317A1 (de) 2011-12-16 2013-06-20 Universitätsklinikum Hamburg-Eppendorf Gerüstfreie Leberzellaggregate zur Verwendung bei der Transplantation
CN102559581B (zh) * 2012-01-20 2014-09-10 四川大学华西医院 一种无血清肝细胞培养基
WO2014050139A1 (ja) 2012-09-27 2014-04-03 株式会社クラレ サイトカインがチトクロムｐ４５０の代謝能に及ぼす影響を評価する方法および薬剤のスクリーニング方法
US20140106356A1 (en) 2012-10-12 2014-04-17 National Taiwan University Kit for drug metabolism determination and toxicity prediction
US9442105B2 (en) * 2013-03-15 2016-09-13 Organovo, Inc. Engineered liver tissues, arrays thereof, and methods of making the same
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JP6681664B2 (ja) * 2014-09-05 2020-04-15 株式会社日本触媒 含フッ素ポリイミドを含む酸素ガス透過性の細胞培養用基材、該基材を備えた細胞培養用容器、及び、該基材を用いた細胞培養方法

Also Published As

Publication number Publication date
JP6810742B2 (ja) 2021-01-06
US20210269774A1 (en) 2021-09-02
JP2019506134A (ja) 2019-03-07
US11981926B2 (en) 2024-05-14
EP3287521B1 (en) 2021-06-23
JP7097942B2 (ja) 2022-07-08
EP3287521A1 (en) 2018-02-28
US20190119647A1 (en) 2019-04-25
RU2018111219A3 (enExample) 2019-09-30
WO2018036910A1 (en) 2018-03-01
RU2018111219A (ru) 2019-09-30
US11041145B2 (en) 2021-06-22
EP3940059A1 (en) 2022-01-19
JP2021048865A (ja) 2021-04-01
PL3287521T3 (pl) 2021-12-20
CN108138135A (zh) 2018-06-08

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