RU2018111219A - Культура клеток - Google Patents

Культура клеток Download PDF

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Publication number
RU2018111219A
RU2018111219A RU2018111219A RU2018111219A RU2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A
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RU
Russia
Prior art keywords
dimensional
complete medium
spheroid
ali
culture
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RU2018111219A
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English (en)
Russian (ru)
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RU2018111219A3 (enExample
Inventor
Давид БОВАР
Карста ЛЮТТИХ
Original Assignee
Филип Моррис Продактс С.А.
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Application filed by Филип Моррис Продактс С.А. filed Critical Филип Моррис Продактс С.А.
Publication of RU2018111219A3 publication Critical patent/RU2018111219A3/ru
Publication of RU2018111219A publication Critical patent/RU2018111219A/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/27—Lung cells, respiratory tract cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Sustainable Development (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
RU2018111219A 2016-08-25 2017-08-17 Культура клеток RU2018111219A (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16185725.5 2016-08-25
EP16185725.5A EP3287521B1 (en) 2016-08-25 2016-08-25 Cell culture
PCT/EP2017/070881 WO2018036910A1 (en) 2016-08-25 2017-08-17 Cell culture

Publications (2)

Publication Number Publication Date
RU2018111219A3 RU2018111219A3 (enExample) 2019-09-30
RU2018111219A true RU2018111219A (ru) 2019-09-30

Family

ID=56842661

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2018111219A RU2018111219A (ru) 2016-08-25 2017-08-17 Культура клеток

Country Status (8)

Country Link
US (2) US11041145B2 (enExample)
EP (2) EP3940059A1 (enExample)
JP (2) JP6810742B2 (enExample)
KR (1) KR20180049000A (enExample)
CN (1) CN108138135A (enExample)
PL (1) PL3287521T3 (enExample)
RU (1) RU2018111219A (enExample)
WO (1) WO2018036910A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES3070913T3 (en) * 2018-05-16 2026-06-18 Inst Nat Sante Rech Med Method of culturing proliferative hepatocytes
EP3927417A4 (en) * 2019-02-19 2022-11-16 Iontox LLC METHOD FOR PREDICTING REPEATED-DOSE TOXICITY USING AN INTEGRATED ORGAN PLATFORM
CN113699202A (zh) * 2020-05-21 2021-11-26 华子昂 一种用混合细胞与人工细胞培养巢制备胶原蛋白的方法
WO2023282925A1 (en) * 2021-07-08 2023-01-12 Ourotech, Inc. Treatment efficacy prediction systems and methods
CN120005810A (zh) * 2025-04-18 2025-05-16 立沃生物科技（深圳）有限公司 肝祖细胞、应用及3d动态培养扩增方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2313377B (en) 1996-05-20 1998-07-01 Sumitomo Chemical Co Kit for diagnosis of hepatitis C and alcoholic cirrhosis
GB9913979D0 (en) 1999-06-17 1999-08-18 Univ Wales Medicine Spheroid preparation
US7351584B2 (en) 2002-02-26 2008-04-01 In Vitro Technologies, Inc. Hepatocyte bioreactor system for long term culture of functional hepatocyte spheroids
EP1496066A4 (en) 2002-03-25 2006-09-13 Japan Science & Tech Agency ANTIBODY RECOGNIZING PROLIFERATIVE HUMAN LIVER CELLS, PROLIFERATIVE HUMAN LIVER CELLS, AND FUNCTIONAL HUMAN LIVER CELLS
US7753192B2 (en) 2005-12-23 2010-07-13 The Coca-Cola Company Apparatus and method for orienting spheroidal containers and packaging beverages in spheroidal containers
JP5217220B2 (ja) 2007-04-12 2013-06-19 株式会社日立製作所 細胞分離装置
CA2693481A1 (en) * 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
EP2334804B1 (en) 2008-10-07 2013-12-04 True North Therapeutics, Inc. Co-culture compositions and methods
WO2010079602A1 (ja) 2009-01-08 2010-07-15 株式会社日立製作所 動物肝細胞の培養方法
GB201104511D0 (en) * 2011-03-17 2011-05-04 Univ Cardiff Cell based screening assay
JP5812816B2 (ja) * 2011-11-15 2015-11-17 コバレントマテリアル株式会社 細胞培養担体及びその製造方法
DE102011121317A1 (de) 2011-12-16 2013-06-20 Universitätsklinikum Hamburg-Eppendorf Gerüstfreie Leberzellaggregate zur Verwendung bei der Transplantation
CN102559581B (zh) * 2012-01-20 2014-09-10 四川大学华西医院 一种无血清肝细胞培养基
WO2014050139A1 (ja) 2012-09-27 2014-04-03 株式会社クラレ サイトカインがチトクロムｐ４５０の代謝能に及ぼす影響を評価する方法および薬剤のスクリーニング方法
US20140106356A1 (en) 2012-10-12 2014-04-17 National Taiwan University Kit for drug metabolism determination and toxicity prediction
US9442105B2 (en) * 2013-03-15 2016-09-13 Organovo, Inc. Engineered liver tissues, arrays thereof, and methods of making the same
US11447743B2 (en) 2014-04-22 2022-09-20 Nippon Shokubai Co., Ltd. Cell culture substrate comprising fluorine-containing polymer on its surface
JP6681664B2 (ja) * 2014-09-05 2020-04-15 株式会社日本触媒 含フッ素ポリイミドを含む酸素ガス透過性の細胞培養用基材、該基材を備えた細胞培養用容器、及び、該基材を用いた細胞培養方法

Also Published As

Publication number Publication date
JP6810742B2 (ja) 2021-01-06
US20210269774A1 (en) 2021-09-02
KR20180049000A (ko) 2018-05-10
JP2019506134A (ja) 2019-03-07
US11981926B2 (en) 2024-05-14
EP3287521B1 (en) 2021-06-23
JP7097942B2 (ja) 2022-07-08
EP3287521A1 (en) 2018-02-28
US20190119647A1 (en) 2019-04-25
RU2018111219A3 (enExample) 2019-09-30
WO2018036910A1 (en) 2018-03-01
US11041145B2 (en) 2021-06-22
EP3940059A1 (en) 2022-01-19
JP2021048865A (ja) 2021-04-01
PL3287521T3 (pl) 2021-12-20
CN108138135A (zh) 2018-06-08

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