JP6810742B2 - 細胞培養 - Google Patents

細胞培養 Download PDF

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JP6810742B2
JP6810742B2 JP2018519751A JP2018519751A JP6810742B2 JP 6810742 B2 JP6810742 B2 JP 6810742B2 JP 2018519751 A JP2018519751 A JP 2018519751A JP 2018519751 A JP2018519751 A JP 2018519751A JP 6810742 B2 JP6810742 B2 JP 6810742B2
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complete
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william
pneumacult
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JP2019506134A5 (enExample
JP2019506134A (ja
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ダヴィド ボヴァール
ダヴィド ボヴァール
カルスタ リュティヒ
カルスタ リュティヒ
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フィリップ・モーリス・プロダクツ・ソシエテ・アノニム
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/27—Lung cells, respiratory tract cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Sustainable Development (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2018519751A 2016-08-25 2017-08-17 細胞培養 Active JP6810742B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16185725.5A EP3287521B1 (en) 2016-08-25 2016-08-25 Cell culture
EP16185725.5 2016-08-25
PCT/EP2017/070881 WO2018036910A1 (en) 2016-08-25 2017-08-17 Cell culture

Related Child Applications (1)

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JP2020205752A Division JP7097942B2 (ja) 2016-08-25 2020-12-11 細胞培養

Publications (3)

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JP2019506134A JP2019506134A (ja) 2019-03-07
JP2019506134A5 JP2019506134A5 (enExample) 2020-07-16
JP6810742B2 true JP6810742B2 (ja) 2021-01-06

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JP2018519751A Active JP6810742B2 (ja) 2016-08-25 2017-08-17 細胞培養
JP2020205752A Active JP7097942B2 (ja) 2016-08-25 2020-12-11 細胞培養

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US (2) US11041145B2 (enExample)
EP (2) EP3940059A1 (enExample)
JP (2) JP6810742B2 (enExample)
KR (1) KR20180049000A (enExample)
CN (1) CN108138135A (enExample)
PL (1) PL3287521T3 (enExample)
RU (1) RU2018111219A (enExample)
WO (1) WO2018036910A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3794107A1 (en) * 2018-05-16 2021-03-24 Institut National de la Santé et de la Recherche Médicale (INSERM) Method of culturing proliferative hepatocytes
EP3927417A4 (en) * 2019-02-19 2022-11-16 Iontox LLC METHODS FOR PREDICTING REPEATED DOSE TOXICITY USING AN INTEGRATED ORGAN PLATFORM
CN113699202A (zh) * 2020-05-21 2021-11-26 华子昂 一种用混合细胞与人工细胞培养巢制备胶原蛋白的方法
EP4366873A4 (en) * 2021-07-08 2025-08-06 Ourotech Inc SYSTEMS AND METHODS FOR PREDICTING TREATMENT EFFECTIVENESS
CN120005810A (zh) * 2025-04-18 2025-05-16 立沃生物科技（深圳）有限公司 肝祖细胞、应用及3d动态培养扩增方法

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
GB2313377B (en) 1996-05-20 1998-07-01 Sumitomo Chemical Co Kit for diagnosis of hepatitis C and alcoholic cirrhosis
GB9913979D0 (en) 1999-06-17 1999-08-18 Univ Wales Medicine Spheroid preparation
US7351584B2 (en) 2002-02-26 2008-04-01 In Vitro Technologies, Inc. Hepatocyte bioreactor system for long term culture of functional hepatocyte spheroids
WO2003080821A1 (en) 2002-03-25 2003-10-02 Japan Science And Technology Agency Method of proliferating human hepatocytes and method of obtaining human hepatocytes
US7753192B2 (en) 2005-12-23 2010-07-13 The Coca-Cola Company Apparatus and method for orienting spheroidal containers and packaging beverages in spheroidal containers
JP5217220B2 (ja) 2007-04-12 2013-06-19 株式会社日立製作所 細胞分離装置
SG183067A1 (en) * 2007-07-20 2012-08-30 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
US20110262956A1 (en) 2008-10-07 2011-10-27 Guillermo Munoz Elias Co-culture compositions and methods
JP5337169B2 (ja) 2009-01-08 2013-11-06 株式会社日立製作所 動物肝細胞の培養方法
GB201104511D0 (en) * 2011-03-17 2011-05-04 Univ Cardiff Cell based screening assay
JP5812816B2 (ja) * 2011-11-15 2015-11-17 コバレントマテリアル株式会社 細胞培養担体及びその製造方法
DE102011121317A1 (de) 2011-12-16 2013-06-20 Universitätsklinikum Hamburg-Eppendorf Gerüstfreie Leberzellaggregate zur Verwendung bei der Transplantation
CN102559581B (zh) * 2012-01-20 2014-09-10 四川大学华西医院 一种无血清肝细胞培养基
US10677783B2 (en) 2012-09-27 2020-06-09 Corning Incorporated Method for evaluating effect of cytokine on metabolic activity of cytochrome P450, and drug screening method
US20140106356A1 (en) 2012-10-12 2014-04-17 National Taiwan University Kit for drug metabolism determination and toxicity prediction
US9442105B2 (en) * 2013-03-15 2016-09-13 Organovo, Inc. Engineered liver tissues, arrays thereof, and methods of making the same
WO2015163043A1 (ja) 2014-04-22 2015-10-29 株式会社日本触媒 フッ素含有ポリマーを表面に含む細胞培養用基材
JP6681664B2 (ja) * 2014-09-05 2020-04-15 株式会社日本触媒 含フッ素ポリイミドを含む酸素ガス透過性の細胞培養用基材、該基材を備えた細胞培養用容器、及び、該基材を用いた細胞培養方法

Also Published As

Publication number Publication date
CN108138135A (zh) 2018-06-08
JP7097942B2 (ja) 2022-07-08
US20190119647A1 (en) 2019-04-25
WO2018036910A1 (en) 2018-03-01
JP2021048865A (ja) 2021-04-01
US20210269774A1 (en) 2021-09-02
EP3940059A1 (en) 2022-01-19
KR20180049000A (ko) 2018-05-10
US11041145B2 (en) 2021-06-22
PL3287521T3 (pl) 2021-12-20
RU2018111219A (ru) 2019-09-30
EP3287521A1 (en) 2018-02-28
EP3287521B1 (en) 2021-06-23
RU2018111219A3 (enExample) 2019-09-30
US11981926B2 (en) 2024-05-14
JP2019506134A (ja) 2019-03-07

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