KR20170055791A - 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법 - Google Patents

인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법 Download PDF

Info

Publication number
KR20170055791A
KR20170055791A KR1020150159011A KR20150159011A KR20170055791A KR 20170055791 A KR20170055791 A KR 20170055791A KR 1020150159011 A KR1020150159011 A KR 1020150159011A KR 20150159011 A KR20150159011 A KR 20150159011A KR 20170055791 A KR20170055791 A KR 20170055791A
Authority
KR
South Korea
Prior art keywords
activity
expression
fibroblast
fibroblasts
mmp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020150159011A
Other languages
English (en)
Korean (ko)
Inventor
김상헌
최종훈
박귀덕
Original Assignee
한국과학기술연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국과학기술연구원 filed Critical 한국과학기술연구원
Priority to KR1020150159011A priority Critical patent/KR20170055791A/ko
Priority to JP2018503548A priority patent/JP6933378B2/ja
Priority to CN202110909867.9A priority patent/CN113583946B/zh
Priority to US15/560,482 priority patent/US10725043B2/en
Priority to PCT/KR2016/004043 priority patent/WO2017082491A1/ko
Priority to EP16864417.7A priority patent/EP3375865A4/en
Priority to CN201680003627.XA priority patent/CN107002032B/zh
Publication of KR20170055791A publication Critical patent/KR20170055791A/ko
Priority to JP2021131745A priority patent/JP7235350B2/ja
Priority to JP2023022415A priority patent/JP7525939B2/ja
Priority to JP2024111522A priority patent/JP7727339B2/ja
Ceased legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • G01N33/5743—
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5751—Immunoassay; Biospecific binding assay; Materials therefor for cancer of the skin, e.g. melanoma

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Pain & Pain Management (AREA)
  • Physical Education & Sports Medicine (AREA)
KR1020150159011A 2015-11-12 2015-11-12 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법 Ceased KR20170055791A (ko)

Priority Applications (10)

Application Number Priority Date Filing Date Title
KR1020150159011A KR20170055791A (ko) 2015-11-12 2015-11-12 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
EP16864417.7A EP3375865A4 (en) 2015-11-12 2016-04-19 THREE DIMENSIONAL FIBROBLAST AGGREGATE AND IN VITRO 3D SKIN MODEL THEREOF
CN202110909867.9A CN113583946B (zh) 2015-11-12 2016-04-19 三维成纤维细胞集合体及包含此的体外三维皮肤真皮模型
US15/560,482 US10725043B2 (en) 2015-11-12 2016-04-19 Three-dimensional fibroblast aggregate and in vitro 3D skin dermis model comprising same
PCT/KR2016/004043 WO2017082491A1 (ko) 2015-11-12 2016-04-19 3차원 섬유아세포집합체 및 그를 포함하는 인 비트로 3d 피부 진피 모델
JP2018503548A JP6933378B2 (ja) 2015-11-12 2016-04-19 三次元線維芽細胞集合体、及びそれを含むインビトロ３ｄ皮膚真皮モデル
CN201680003627.XA CN107002032B (zh) 2015-11-12 2016-04-19 三维成纤维细胞集合体及包含此的体外三维皮肤真皮模型
JP2021131745A JP7235350B2 (ja) 2015-11-12 2021-08-12 マルトース結合タンパク質リンカを用いた三次元線維芽細胞集合体、及びそれを含むインビトロ３ｄ皮膚真皮モデル
JP2023022415A JP7525939B2 (ja) 2015-11-12 2023-02-16 Ｍｂｐ－ｆｇｆ組み換えタンパク質を用いた三次元線維芽細胞集合体の調製、及び当該線維芽細胞集合体を含むインビトロ３ｄ皮膚真皮モデル
JP2024111522A JP7727339B2 (ja) 2015-11-12 2024-07-11 コラーゲン発現量が制御された三次元線維芽細胞集合体を生成する方法、及び当該線維芽細胞集合体を含むインビトロ３ｄ皮膚真皮モデル

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150159011A KR20170055791A (ko) 2015-11-12 2015-11-12 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020170156513A Division KR20170132121A (ko) 2017-11-22 2017-11-22 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법

Publications (1)

Publication Number Publication Date
KR20170055791A true KR20170055791A (ko) 2017-05-22

Family

ID=58695687

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020150159011A Ceased KR20170055791A (ko) 2015-11-12 2015-11-12 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법

Country Status (6)

Country Link
US (1) US10725043B2 (https=)
EP (1) EP3375865A4 (https=)
JP (4) JP6933378B2 (https=)
KR (1) KR20170055791A (https=)
CN (2) CN113583946B (https=)
WO (1) WO2017082491A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019004793A3 (ko) * 2017-06-30 2019-03-28 (주)아모레퍼시픽 인공피부 제조방법 및 인공피부
KR102365125B1 (ko) * 2020-08-12 2022-02-18 주식회사 티앤알바이오팹 인공 피부 모델을 사용한 피부 탄성 측정 방법 및 피부 첨가제의 스크리닝 혹은 선별 방법

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170055791A (ko) * 2015-11-12 2017-05-22 한국과학기술연구원 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
EP3708653A4 (en) * 2017-11-10 2021-08-18 Toppan Printing Co., Ltd. ARTIFICIAL ADAPTIVE TISSUE AND METHOD FOR MANUFACTURING THEREOF, METHOD FOR MANUFACTURING ARTIFICIAL SKIN AND AGENTS FOR FAT CELLS
CN108795867A (zh) * 2018-06-05 2018-11-13 华东理工大学 用于构建结肠癌细胞腹膜转移体外三维模型的方法
WO2022024886A1 (ja) * 2020-07-27 2022-02-03 株式会社コーセー 全分泌調節剤の評価及び／又は選択方法
CN114107429A (zh) * 2021-12-02 2022-03-01 云南贝泰妮生物科技集团股份有限公司 一种化妆品原料体外3d皮肤模型屏障修复功效的筛选方法
CN114292894B (zh) * 2022-01-14 2023-08-11 广东悠质检测技术有限公司 一种用体外细胞评价化妆品及原料紧致功效的方法
CN119286771A (zh) * 2024-10-28 2025-01-10 深圳锦时生物科技有限公司 一种高效分离自体皮肤来源的成纤维细胞培养方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03266980A (ja) 1989-03-16 1991-11-27 W R Grace & Co 細胞培養用基材およびそれを用いた細胞集合体の製造方法
JPH0489000A (ja) * 1990-05-02 1992-03-23 W R Grace & Co 細胞培養キットおよび細胞傷害試験法
JP4751005B2 (ja) * 2000-05-31 2011-08-17 フラウンホファー ゲセルシャフト ツール フェールデルンク ダー アンゲヴァンテン フォルシュンク エー．ファオ． 三次元皮膚モデル
JP3709369B2 (ja) 2001-11-13 2005-10-26 丸善製薬株式会社 皮膚化粧料及び美容用飲食品
DE102004039537A1 (de) * 2004-08-13 2006-02-23 Phenion Gmbh & Co. Kg Vernetzte Kollagenmatrix zur Herstellung eines Hautäquivalentes
JP4792566B2 (ja) 2005-06-01 2011-10-12 財団法人ヒューマンサイエンス振興財団 樹状細胞を含む三次元培養ヒト皮膚モデル
JP2010172240A (ja) * 2009-01-28 2010-08-12 Shiseido Co Ltd エラスチン発現低下を回復する薬剤のスクリーニング方法
JP2010193822A (ja) 2009-02-26 2010-09-09 Toyobo Co Ltd ３次元培養細胞により作成した皮膚同等物およびそれを用いる紫外線の影響の評価方法
KR101094445B1 (ko) 2009-05-13 2011-12-15 한국과학기술연구원 줄기세포에 대한 접착활성을 갖는 섬유아세포 성장인자의 재조합 단백질 및 이를 이용한 줄기세포의 배양방법
JP2012050406A (ja) 2010-09-03 2012-03-15 Genesis Healthcare Kk 皮膚関連遺伝子の検出方法、並びに該遺伝子を用いた肌情報の提供方法、化粧品の販売システム、化粧品の販売方法及び化粧品成分のスクリーニング方法
CN104263698B (zh) * 2014-09-19 2016-05-18 江苏华亿细胞组织工程有限公司 临床治疗级细胞治疗用成纤维细胞规模化制备人类细胞外基质筛选培养方法
KR20170055791A (ko) 2015-11-12 2017-05-22 한국과학기술연구원 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019004793A3 (ko) * 2017-06-30 2019-03-28 (주)아모레퍼시픽 인공피부 제조방법 및 인공피부
KR102365125B1 (ko) * 2020-08-12 2022-02-18 주식회사 티앤알바이오팹 인공 피부 모델을 사용한 피부 탄성 측정 방법 및 피부 첨가제의 스크리닝 혹은 선별 방법

Also Published As

Publication number Publication date
JP2024133695A (ja) 2024-10-02
CN107002032B (zh) 2021-08-27
CN107002032A (zh) 2017-08-01
JP7525939B2 (ja) 2024-07-31
JP7727339B2 (ja) 2025-08-21
JP2023058684A (ja) 2023-04-25
US10725043B2 (en) 2020-07-28
US20180252715A1 (en) 2018-09-06
JP2018535646A (ja) 2018-12-06
JP7235350B2 (ja) 2023-03-08
EP3375865A1 (en) 2018-09-19
CN113583946B (zh) 2024-11-08
WO2017082491A1 (ko) 2017-05-18
JP6933378B2 (ja) 2021-09-08
EP3375865A4 (en) 2019-07-10
JP2021180681A (ja) 2021-11-25
CN113583946A (zh) 2021-11-02

Similar Documents

Publication Publication Date Title
KR20170055791A (ko) 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
Chin et al. A hydrogel-integrated culture device to interrogate T cell activation with physicochemical cues
Missirlis et al. Combined effects of PEG hydrogel elasticity and cell-adhesive coating on fibroblast adhesion and persistent migration
Wang et al. Efficient capture of cancer cells by their replicated surfaces reveals multiscale topographic interactions coupled with molecular recognition
Shroff et al. Enhanced integrin mediated signaling and cell cycle progression on fibronectin mimetic peptide amphiphile monolayers
Hu et al. Protein–substrate adhesion in microcontact printing regulates cell behavior
Cai et al. Biomaterial stiffness guides cross-talk between chondrocytes: implications for a novel cellular response in cartilage tissue engineering
Menon et al. Microfluidic assay to study the combinatorial impact of substrate properties on mesenchymal stem cell migration
Yeung et al. Reversible self-assembled monolayers with tunable surface dynamics for controlling cell adhesion behavior
Mnatsakanyan et al. Controlled assembly of fibronectin nanofibrils triggered by random copolymer chemistry
Seras-Franzoso et al. Bioadhesiveness and efficient mechanotransduction stimuli synergistically provided by bacterial inclusion bodies as scaffolds for tissue engineering
Noiri et al. Exogenous cell surface modification with cell penetrating peptide-conjugated lipids causes spontaneous cell adhesion
KR101919930B1 (ko) 인 비트로 3차원 세포 배양 모델, 이의 제조 방법 및 이를 이용한 스크리닝 방법
KR102066670B1 (ko) 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
KR20170132121A (ko) 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
Araújo et al. Surfaces mimicking glycosaminoglycans trigger different response of stem cells via distinct fibronectin adsorption and reorganization
Thomas et al. Layer-by-layer assembly of heparin and peptide-polyethylene glycol conjugates to form hybrid nanothin films of biomatrices
Morimoto et al. Latent nature of collagen in promoting three-dimensional adherent spheroid formation of fibroblasts
Chen et al. Decoupling of density-dependent migration/proliferation dichotomy on surface potential gradient
Ahmed et al. Poly (vinylmethylsiloxane) elastomer networks as functional materials for cell adhesion and migration studies
HK40056234B (zh) 三维成纤维细胞集合体及包含此的体外三维皮肤真皮模型
Pulsipher et al. In situ modulation of cell behavior via smart dual-ligand surfaces
HK40056234A (en) Three-dimensional fibroblast aggregate and in vitro 3d skin dermis model comprising same
KR102261934B1 (ko) Mbp-fgf2를 이용한 3차원 섬유아세포집합체를 제조하는 방법
Patkunarajah Mechanoelectrical signalling via ELKIN1 and PIEZO1 in cell lines derived from metastatic melanoma

Legal Events

Date Code Title Description
A201 Request for examination
PA0109 Patent application

St.27 status event code: A-0-1-A10-A12-nap-PA0109

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000

D14-X000 Search report completed

St.27 status event code: A-1-2-D10-D14-srh-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E601 Decision to refuse application
PE0601 Decision on rejection of patent

St.27 status event code: N-2-6-B10-B15-exm-PE0601

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

E601 Decision to refuse application
E801 Decision on dismissal of amendment
PE0601 Decision on rejection of patent

St.27 status event code: N-2-6-B10-B15-exm-PE0601

PE0801 Dismissal of amendment

St.27 status event code: A-2-2-P10-P12-nap-PE0801

A107 Divisional application of patent
PA0107 Divisional application

St.27 status event code: A-0-1-A10-A18-div-PA0107

St.27 status event code: A-0-1-A10-A16-div-PA0107

P22-X000 Classification modified

St.27 status event code: A-2-2-P10-P22-nap-X000

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

P22-X000 Classification modified

St.27 status event code: A-2-2-P10-P22-nap-X000