KR20110057188A - 바이오마커 프로파일 측정 시스템 및 방법 - Google Patents
바이오마커 프로파일 측정 시스템 및 방법 Download PDFInfo
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- KR20110057188A KR20110057188A KR1020117007024A KR20117007024A KR20110057188A KR 20110057188 A KR20110057188 A KR 20110057188A KR 1020117007024 A KR1020117007024 A KR 1020117007024A KR 20117007024 A KR20117007024 A KR 20117007024A KR 20110057188 A KR20110057188 A KR 20110057188A
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| KR101483284B1 (ko) * | 2013-01-31 | 2015-01-15 | 한국과학기술원 | 질병 관련 단일염기다형성 조합 추출 방법, 질병 발생 위험도 예측 방법, 그리고 이를 이용한 질병 발생 위험도 예측 장치 |
| KR20200086627A (ko) * | 2017-11-13 | 2020-07-17 | 더 멀티플 마이얼로머 리서치 파운데이션, 인크. | 통합된, 분자, 체학, 면역요법, 대사, 후성적, 및 임상 데이터베이스 |
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| US8655807B2 (en) * | 2010-04-05 | 2014-02-18 | Applied Research Associates, Inc. | Methods for forming recognition algorithms for laser-induced breakdown spectroscopy |
| US8676739B2 (en) | 2010-11-11 | 2014-03-18 | International Business Machines Corporation | Determining a preferred node in a classification and regression tree for use in a predictive analysis |
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| WO2015027116A1 (en) * | 2013-08-21 | 2015-02-26 | The Regents Of The University Of California | Diagnostic and predictive metabolite patterns for disorders affecting the brain and nervous system |
| EP3074525B1 (en) | 2013-11-26 | 2024-06-26 | University of North Texas Health Science Center at Fort Worth | Personalized medicine approach for treating cognitive loss |
| WO2022187670A1 (en) * | 2021-03-05 | 2022-09-09 | University Of North Texas Health Science Center At Fort Worth | Personalized medicine approach for treating cognitive loss |
| US9545227B2 (en) * | 2013-12-13 | 2017-01-17 | Vital Connect, Inc. | Sleep apnea syndrome (SAS) screening using wearable devices |
| JP6882776B2 (ja) * | 2015-11-12 | 2021-06-02 | 国立大学法人九州大学 | うつ病診断用バイオマーカー及びその使用 |
| US11205103B2 (en) | 2016-12-09 | 2021-12-21 | The Research Foundation for the State University | Semisupervised autoencoder for sentiment analysis |
| US20190354814A1 (en) * | 2017-01-08 | 2019-11-21 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Systems and methods for using supervised learning to predict subject-specific bacteremia outcomes |
| MX2019010295A (es) | 2017-03-01 | 2019-11-21 | Genentech Inc | Métodos de diagnóstico y terapéuticos para el cáncer. |
| TWI640018B (zh) * | 2017-03-15 | 2018-11-01 | 長庚醫療財團法人林口長庚紀念醫院 | Data integration method |
| JP7170250B2 (ja) * | 2017-07-07 | 2022-11-14 | パナソニックＩｐマネジメント株式会社 | 情報提供方法、情報処理システム、及び情報処理方法 |
| CA3019970A1 (en) * | 2018-10-05 | 2020-04-05 | Fang Liu | Methods for diagnosing or treating post-traumatic stress disorder, and compositions therefor |
| US11537888B2 (en) * | 2019-05-15 | 2022-12-27 | The Florida International University Board Of Trustees | Systems and methods for predicting pain level |
| CN113210264B (zh) * | 2021-05-19 | 2023-09-05 | 江苏鑫源烟草薄片有限公司 | 烟草杂物剔除方法及装置 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190857B1 (en) * | 1997-03-24 | 2001-02-20 | Urocor, Inc. | Diagnosis of disease state using MRNA profiles in peripheral leukocytes |
| US5958688A (en) * | 1997-04-28 | 1999-09-28 | The Trustees Of The University Of Pennsylvania | Characterization of mRNA patterns in neurites and single cells for medical diagnosis and therapeutics |
| NO972006D0 (no) * | 1997-04-30 | 1997-04-30 | Forskningsparken I Aas As | Ny metode for diagnose av sykdommer |
| US20070178475A1 (en) * | 1998-09-17 | 2007-08-02 | Nehls Michael C | Novel human polynucleotides and polypeptides encoded thereby |
| WO2000040749A2 (en) * | 1999-01-06 | 2000-07-13 | Genenews Inc. | Method for the detection of gene transcripts in blood and uses thereof |
| DE10019058A1 (de) * | 2000-04-06 | 2001-12-20 | Epigenomics Ag | Detektion von Variationen des DNA-Methylierungsprofils |
| US7329489B2 (en) * | 2000-04-14 | 2008-02-12 | Matabolon, Inc. | Methods for drug discovery, disease treatment, and diagnosis using metabolomics |
| ES2305234T3 (es) * | 2001-02-27 | 2008-11-01 | Blanchette Rockefeller Neurosciences Institute | Diagnostico de la enfermedad de alzheimer basado en la fosforilacion de una proteina quinosa activada por mitogeno. |
| JP3771502B2 (ja) * | 2002-02-14 | 2006-04-26 | 国立大学法人 新潟大学 | 精神分裂病により発現量が変化する遺伝子を規定する核酸を解析する方法 |
| AU2003250033A1 (en) * | 2002-07-11 | 2004-02-02 | Novartis Ag | Genes associated with schizophrenia, adhd and bipolar disorders |
| JP2004135667A (ja) * | 2002-09-27 | 2004-05-13 | Japan Science & Technology Agency | 血液を用いた統合失調症の診断方法 |
| JP2004208547A (ja) * | 2002-12-27 | 2004-07-29 | Hitachi Ltd | うつ病の評価方法 |
| US20040248286A1 (en) * | 2003-03-21 | 2004-12-09 | Christine Konradi | Nucleic acid molecules that are differentially regulated in a bipolar disorder and uses thereof |
| US20070105105A1 (en) * | 2003-05-23 | 2007-05-10 | Mount Sinai School Of Medicine Of New York University | Surrogate cell gene expression signatures for evaluating the physical state of a subject |
| US20060150264A1 (en) * | 2003-06-13 | 2006-07-06 | Sabine Bahn | Differential gene expression in schizophrenia |
| US20050069936A1 (en) * | 2003-09-26 | 2005-03-31 | Cornelius Diamond | Diagnostic markers of depression treatment and methods of use thereof |
| US20050209181A1 (en) * | 2003-11-05 | 2005-09-22 | Huda Akil | Compositions and methods for diagnosing and treating mental disorders |
| US20050208519A1 (en) * | 2004-03-12 | 2005-09-22 | Genenews Inc. | Biomarkers for diagnosing schizophrenia and bipolar disorder |
| JP2005312435A (ja) * | 2004-03-29 | 2005-11-10 | Kazuhito Rokutan | うつ病の評価方法 |
| EP1766014A4 (en) * | 2004-06-03 | 2008-12-10 | Athlomics Pty Ltd | AGENTS AND METHODS FOR THE DIAGNOSIS OF STRESS |
| ES2660765T3 (es) * | 2004-06-21 | 2018-03-26 | The Board Of Trustees Of The Leland Stanford Junior University | Genes y rutas expresadas de forma diferencial en trastorno bipolar y/o trastorno depresivo mayor |
| US7736621B2 (en) * | 2004-08-03 | 2010-06-15 | Hypatia Ltd. | Methods for gauging the effect of a depression treatment by determining the levels of beta-arrestin 1 and G-protein coupled receptor kinase 2 |
| WO2006097244A2 (en) * | 2005-03-17 | 2006-09-21 | F. Hoffman-La Roche Ag | Methods for assessing emphysema |
| US20070122395A1 (en) * | 2005-10-06 | 2007-05-31 | Vanderbilt University | Genetic and pharmacological regulation of antidepressant-sensitive biogenic amine transporters through PKG/p38 map kinase |
| US7906281B2 (en) * | 2005-10-07 | 2011-03-15 | The Regents Of The University Of California | Method to predict the response to lithium treatment |
| WO2007044094A1 (en) * | 2005-10-11 | 2007-04-19 | Blanchette Rockefeller Neurosciences Institute | Alzheimer's disease-specific alterations of the erk1/erk2 phosphorylation ratio as alzheimer's disease-specific molecular biomarkers (adsmb) |
| US20080033598A1 (en) * | 2006-03-23 | 2008-02-07 | Hollingsworth Stephen A | Materials management system and method |
| US20070292880A1 (en) * | 2006-05-05 | 2007-12-20 | Robert Philibert | Compositions and methods for detecting predisposition to a substance use disorder or to a mental illness or syndrome |
| US8163475B2 (en) * | 2006-05-18 | 2012-04-24 | The Mclean Hospital Corporation | Methods for diagnosis and prognosis of psychotic disorders |
| WO2008011046A2 (en) * | 2006-07-17 | 2008-01-24 | The H.Lee Moffitt Cancer And Research Institute, Inc. | Computer systems and methods for selecting subjects for clinical trials |
| WO2008079269A2 (en) * | 2006-12-19 | 2008-07-03 | Genego, Inc. | Novel methods for functional analysis of high-throughput experimental data and gene groups identified therfrom |
| WO2008124428A1 (en) * | 2007-04-03 | 2008-10-16 | Indiana University Research And Technology Corporation | Blood biomarkers for mood disorders |
| WO2008144371A1 (en) * | 2007-05-16 | 2008-11-27 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for diagnosing suicidal tendencies |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101483284B1 (ko) * | 2013-01-31 | 2015-01-15 | 한국과학기술원 | 질병 관련 단일염기다형성 조합 추출 방법, 질병 발생 위험도 예측 방법, 그리고 이를 이용한 질병 발생 위험도 예측 장치 |
| KR20200086627A (ko) * | 2017-11-13 | 2020-07-17 | 더 멀티플 마이얼로머 리서치 파운데이션, 인크. | 통합된, 분자, 체학, 면역요법, 대사, 후성적, 및 임상 데이터베이스 |
Also Published As
| Publication number | Publication date |
|---|---|
| EA201071324A1 (ru) | 2011-12-30 |
| CA2728171A1 (en) | 2010-03-04 |
| WO2010025216A1 (en) | 2010-03-04 |
| JP2012501181A (ja) | 2012-01-19 |
| BRPI0914859A2 (pt) | 2015-11-03 |
| WO2010025216A9 (en) | 2010-11-18 |
| CN102224256A (zh) | 2011-10-19 |
| EP2318551A1 (en) | 2011-05-11 |
| AU2009285766A1 (en) | 2010-03-04 |
| EP2318551A4 (en) | 2012-10-24 |
| US20110172501A1 (en) | 2011-07-14 |
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