KR20100103595A - 비-글라이코실화된 단백질의 정제 - Google Patents

비-글라이코실화된 단백질의 정제 Download PDF

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KR20100103595A
KR20100103595A KR1020107015694A KR20107015694A KR20100103595A KR 20100103595 A KR20100103595 A KR 20100103595A KR 1020107015694 A KR1020107015694 A KR 1020107015694A KR 20107015694 A KR20107015694 A KR 20107015694A KR 20100103595 A KR20100103595 A KR 20100103595A
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Prior art keywords
chromatography
hydrophobic
polypeptides
exchange chromatography
charge induction
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Korean (ko)
Inventor
로베르토 팔켄스타인
버지트 베이단즈
클라우디아 기쎌
시빌르 그라이탠너
아델베르트 그로스만
프레더리크 헤쎄
브리지테 크래머
마르크 폼피아티
안드레아스 챠우브마
니콜 푸에흘러
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에프. 호프만-라 로슈 아게
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Publication of KR20100103595A publication Critical patent/KR20100103595A/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020107015694A 2008-01-18 2009-01-15 비-글라이코실화된 단백질의 정제 Ceased KR20100103595A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08000884 2008-01-18
EP08000884.0 2008-01-18

Publications (1)

Publication Number Publication Date
KR20100103595A true KR20100103595A (ko) 2010-09-27

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KR1020107015694A Ceased KR20100103595A (ko) 2008-01-18 2009-01-15 비-글라이코실화된 단백질의 정제

Country Status (9)

Country Link
US (3) US20110034672A1 (enExample)
EP (1) EP2245044A2 (enExample)
JP (2) JP5944101B2 (enExample)
KR (1) KR20100103595A (enExample)
CN (2) CN107033233A (enExample)
AU (1) AU2009204998B2 (enExample)
CA (1) CA2711375C (enExample)
IL (1) IL206158A (enExample)
WO (1) WO2009090056A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180098541A (ko) * 2015-11-09 2018-09-04 바이오로지칼 이 리미티드 생물학적 활성 재조합 캐리어 단백질을 회수하기 위한 산업적 확장성이 있는 방법

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WO2009090056A2 (en) * 2008-01-18 2009-07-23 F. Hoffmann-La Roche Ag Purification of not-glycosylated polypeptides
DE102009032179A1 (de) * 2009-07-07 2011-01-13 Biogenerix Ag Verfahren zur Reinigung von Interferon beta
CN102219848B (zh) * 2011-05-25 2014-01-22 浙江海正药业股份有限公司 重组人干扰素β-1a的纯化方法
US20140154233A1 (en) 2012-12-05 2014-06-05 Csl Limited Method of purifying therapeutic proteins
US10188965B2 (en) 2012-12-05 2019-01-29 Csl Behring Gmbh Hydrophobic charge induction chromatographic depletion of a protein from a solution
CN104710527B (zh) * 2015-02-28 2018-08-24 苏州金盟生物技术有限公司 一种生物制品的内毒素去除方法
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
CA3179177A1 (en) * 2020-12-23 2022-06-30 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
CN113480632B (zh) * 2021-07-30 2023-02-03 宁波人健药业集团股份有限公司 一种在CHO细胞中表达的重组蛋白rhCG纯化工艺
TW202403043A (zh) 2022-03-18 2024-01-16 美商健臻公司 重組人類酸性鞘磷脂酶醫藥組合物及方法
CN117024561B (zh) * 2023-10-10 2024-02-20 哈药集团生物工程有限公司 一种聚乙二醇修饰干扰素的纯化方法

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US5696238A (en) * 1991-08-20 1997-12-09 Chiron Corporation Purified GP120 composition retaining natural conformation
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
DE4329756A1 (de) * 1993-09-03 1995-03-09 Boehringer Ingelheim Int Verfahren zur Herstellung und Reinigung von alpha-Interferon
EP1104809A1 (en) * 1994-04-09 2001-06-06 F. Hoffmann-La Roche Ag Process for producing alpha-interferon
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KR100443890B1 (ko) 2001-04-11 2004-08-09 한미약품 주식회사 재조합 대장균으로부터 인간 성장 호르몬의 정제 방법
CN1233816C (zh) * 2002-04-16 2005-12-28 海南海梁生物高科技有限公司 酵母重组株及IFNα－la干扰素的纯化方法
JP4319979B2 (ja) * 2002-04-26 2009-08-26 ジェネンテック・インコーポレーテッド タンパク質の非アフィニティ精製
ES2391457T3 (es) * 2003-08-13 2012-11-26 Sandoz Ag Procedimiento para la purificación de polipéptidos recombinantes
PT1689777E (pt) 2003-11-05 2007-05-31 Ares Trading Sa Processo para a purificação da proteína de ligação a il-18
ES2333128T3 (es) 2003-12-22 2010-02-17 Ares Trading S.A. Metodo para purificar fsh.
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US7318918B2 (en) * 2004-05-19 2008-01-15 Maxygen, Inc. Interferon-alpha polypeptides and conjugates
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CN1916024A (zh) * 2005-08-17 2007-02-21 海南国栋药物研究所有限公司 高比活人α－2b干扰素突变体序列、酵母表达质粒的构建及菌株筛选和纯化方法
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CN103864915B (zh) * 2012-12-07 2016-06-08 天津未名生物医药有限公司 纯化重组人干扰素α2b的方法和试剂盒
CN104356223B (zh) * 2014-11-26 2017-11-10 安徽安科生物工程（集团）股份有限公司 高纯度重组人干扰素α2b的制备方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180098541A (ko) * 2015-11-09 2018-09-04 바이오로지칼 이 리미티드 생물학적 활성 재조합 캐리어 단백질을 회수하기 위한 산업적 확장성이 있는 방법

Also Published As

Publication number Publication date
CN107033233A (zh) 2017-08-11
US20110034672A1 (en) 2011-02-10
WO2009090056A3 (en) 2010-01-21
US9481706B2 (en) 2016-11-01
JP2014210797A (ja) 2014-11-13
US20140220633A1 (en) 2014-08-07
CA2711375C (en) 2019-04-16
IL206158A0 (en) 2010-12-30
CN101918430A (zh) 2010-12-15
AU2009204998A1 (en) 2009-07-23
EP2245044A2 (en) 2010-11-03
CA2711375A1 (en) 2009-07-23
US20130190478A1 (en) 2013-07-25
IL206158A (en) 2016-09-29
JP2011509963A (ja) 2011-03-31
WO2009090056A2 (en) 2009-07-23
JP5944101B2 (ja) 2016-07-05
AU2009204998B2 (en) 2014-03-27

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