KR20010024296A - 벡터 구조체와 세포 ｄｎａ의 비상동 재조합법에 의한내생 유전자 발현 - Google Patents
벡터 구조체와 세포 ｄｎａ의 비상동 재조합법에 의한내생 유전자 발현 Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
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- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US94122397A | 1997-09-26 | 1997-09-26 | |
| US08/941,223 | 1997-09-26 | ||
| US15964398A | 1998-09-24 | 1998-09-24 | |
| US09/159,643 | 1998-09-24 | ||
| PCT/US1998/020094 WO1999015650A1 (en) | 1997-09-26 | 1998-09-25 | Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20010024296A true KR20010024296A (ko) | 2001-03-26 |
Family
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Family Applications (1)
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|---|---|---|---|
| KR1020007003195A Ceased KR20010024296A (ko) | 1997-09-26 | 1998-09-25 | 벡터 구조체와 세포 ｄｎａ의 비상동 재조합법에 의한내생 유전자 발현 |
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|---|---|
| EP (1) | EP1017803B1 (enExample) |
| JP (1) | JP5377806B2 (enExample) |
| KR (1) | KR20010024296A (enExample) |
| CN (1) | CN100582230C (enExample) |
| AT (1) | ATE471373T1 (enExample) |
| AU (1) | AU750025B2 (enExample) |
| BR (1) | BR9812540A (enExample) |
| CA (1) | CA2304642C (enExample) |
| DE (1) | DE69841723D1 (enExample) |
| DK (1) | DK1017803T3 (enExample) |
| IL (2) | IL135242A0 (enExample) |
| NO (1) | NO20001497L (enExample) |
| NZ (1) | NZ504185A (enExample) |
| WO (1) | WO1999015650A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130055481A (ko) * | 2011-11-18 | 2013-05-28 | 한국생명공학연구원 | 단백질 발현 미세 조절방법 |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6740503B1 (en) | 1997-09-26 | 2004-05-25 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
| WO2001027150A2 (en) | 1999-10-14 | 2001-04-19 | Clontech Laboratories Inc. | Anthozoa derived chromo/fluoroproteins and methods for using the same |
| CN1384881A (zh) * | 1999-02-19 | 2002-12-11 | 阿瑟西斯公司 | 非靶向活化内源基因的组合物和方法 |
| PT2077279E (pt) | 2000-06-28 | 2012-09-14 | Amgen Inc | Moléculas do receptor de linfopoietina estromal tímica e suas utilizações |
| GB0018876D0 (en) | 2000-08-01 | 2000-09-20 | Applied Research Systems | Method of producing polypeptides |
| EP1352060A2 (en) * | 2000-10-30 | 2003-10-15 | Novozymes Biotech, Inc. | Methods for expressing endogenous genes by restriction enzyme mediated integration |
| US7767454B2 (en) | 2001-11-02 | 2010-08-03 | Abt Holding Company | Methods for mutating genes in cells using insertional mutagenesis |
| WO2003054158A2 (en) | 2001-12-19 | 2003-07-03 | The University Of Chicago | Rapidly maturing fluorescent proteins and methods for using the same |
| US7662924B2 (en) | 2002-02-22 | 2010-02-16 | The Board Of Trustees Of The University Of Illinois | Beta chain-associated regulator of apoptosis |
| EP1534819B1 (en) | 2002-08-21 | 2009-12-09 | Revivicor, Inc. | Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| WO2004071462A2 (en) | 2003-02-12 | 2004-08-26 | Johns Hopkins University | Methods and compositions for treatment of viral infections based on tsg101-vps28 interaction |
| US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
| US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| CA2857051A1 (en) | 2004-03-17 | 2005-09-29 | David Ayares | Tissue products derived from animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| JP4755174B2 (ja) | 2004-04-07 | 2011-08-24 | ザ ユニヴァーシティー オヴ シカゴ | 単量体赤色蛍光タンパク質 |
| JP2008507294A (ja) | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | 菌株遺伝子操作による改善されたタンパク質発現のための方法 |
| ES2679282T3 (es) | 2004-10-22 | 2018-08-23 | Revivicor Inc. | Porcinos transgénicos que carecen de cadena ligera de inmunoglobulina endógena |
| PA8660701A1 (es) | 2005-02-04 | 2006-09-22 | Pfizer Prod Inc | Agonistas de pyy y sus usos |
| US20070054360A1 (en) | 2005-05-12 | 2007-03-08 | Zeren Gao | Compositions and methods for modulating immune responses |
| CA2617930A1 (en) | 2005-08-09 | 2007-03-29 | Revivicor, Inc. | Transgenic ungulates expressing ctla4-ig and uses thereof |
| US7749704B2 (en) | 2005-11-01 | 2010-07-06 | Mayo Foundation For Medical Education And Research | Promoter polymorphisms of the BLyS gene and use in diagnostic methods |
| US8014957B2 (en) | 2005-12-15 | 2011-09-06 | Fred Hutchinson Cancer Research Center | Genes associated with progression and response in chronic myeloid leukemia and uses thereof |
| ES2371495T3 (es) | 2006-07-24 | 2012-01-03 | Biorexis Pharmaceutical Corporation | Proteínas de fusión de exendina. |
| US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| EP2615172A1 (en) | 2007-04-27 | 2013-07-17 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| US8679749B2 (en) | 2007-11-01 | 2014-03-25 | The University Of Chicago | Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation |
| WO2009073511A2 (en) | 2007-11-30 | 2009-06-11 | Mayo Foundation For Medical Education And Research | Polymorphisms of the blys gene and use in diagnostic methods |
| WO2011090971A2 (en) | 2010-01-19 | 2011-07-28 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for male reproductive disorders |
| CN102669057B (zh) * | 2011-03-18 | 2014-11-26 | 上海南方模式生物科技发展有限公司 | 一种调控动物内源基因表达的遗传修饰方法 |
| US9957515B2 (en) | 2013-03-15 | 2018-05-01 | Cibus Us Llc | Methods and compositions for targeted gene modification |
| US10052364B2 (en) | 2013-03-15 | 2018-08-21 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for cognitive disorders |
| CA2942407C (en) * | 2014-03-14 | 2023-09-26 | Cibus Us Llc | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
| WO2016081728A1 (en) | 2014-11-19 | 2016-05-26 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for frailty associated with aging |
| EP3802788B1 (en) | 2018-05-15 | 2025-07-02 | Duke University | Systems and methods for genetic manipulation of akkermansia species |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5024939A (en) * | 1987-07-09 | 1991-06-18 | Genentech, Inc. | Transient expression system for producing recombinant protein |
| WO1991006667A1 (en) * | 1989-11-06 | 1991-05-16 | Cell Genesys, Inc. | Production of proteins using homologous recombination |
| US5204939A (en) * | 1989-12-14 | 1993-04-20 | Fujitsu Limited | Rule base processing system and rule evaluation control method therein |
| US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| US6270989B1 (en) * | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
| US5641670A (en) * | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| US5561053A (en) * | 1994-08-05 | 1996-10-01 | Genentech, Inc. | Method for selecting high-expressing host cells |
| CN1384881A (zh) * | 1999-02-19 | 2002-12-11 | 阿瑟西斯公司 | 非靶向活化内源基因的组合物和方法 |
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1998
- 1998-09-25 IL IL13524298A patent/IL135242A0/xx unknown
- 1998-09-25 KR KR1020007003195A patent/KR20010024296A/ko not_active Ceased
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- 1998-09-25 DE DE69841723T patent/DE69841723D1/de not_active Expired - Lifetime
- 1998-09-25 AU AU95816/98A patent/AU750025B2/en not_active Expired
- 1998-09-25 JP JP2000512942A patent/JP5377806B2/ja not_active Expired - Lifetime
- 1998-09-25 WO PCT/US1998/020094 patent/WO1999015650A1/en not_active Ceased
- 1998-09-25 AT AT98949508T patent/ATE471373T1/de not_active IP Right Cessation
- 1998-09-25 BR BR9812540-0A patent/BR9812540A/pt not_active IP Right Cessation
- 1998-09-25 CN CN98811571A patent/CN100582230C/zh not_active Expired - Lifetime
- 1998-09-25 NZ NZ504185A patent/NZ504185A/xx unknown
- 1998-09-25 DK DK98949508.0T patent/DK1017803T3/da active
- 1998-09-25 EP EP98949508A patent/EP1017803B1/en not_active Expired - Lifetime
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2000
- 2000-03-22 NO NO20001497A patent/NO20001497L/no not_active Application Discontinuation
- 2000-03-23 IL IL135242A patent/IL135242A/en not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130055481A (ko) * | 2011-11-18 | 2013-05-28 | 한국생명공학연구원 | 단백질 발현 미세 조절방법 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999015650A9 (en) | 2000-09-08 |
| ATE471373T1 (de) | 2010-07-15 |
| CA2304642A1 (en) | 1999-04-01 |
| CN100582230C (zh) | 2010-01-20 |
| AU750025B2 (en) | 2002-07-11 |
| JP5377806B2 (ja) | 2013-12-25 |
| HK1027375A1 (en) | 2001-01-12 |
| EP1017803B1 (en) | 2010-06-16 |
| BR9812540A (pt) | 2001-12-26 |
| IL135242A (en) | 2011-06-30 |
| CA2304642C (en) | 2014-11-18 |
| JP2003535564A (ja) | 2003-12-02 |
| NO20001497D0 (no) | 2000-03-22 |
| CN1280615A (zh) | 2001-01-17 |
| EP1017803A4 (en) | 2002-01-09 |
| EP1017803A1 (en) | 2000-07-12 |
| NZ504185A (en) | 2003-02-28 |
| DK1017803T3 (da) | 2010-08-23 |
| WO1999015650A1 (en) | 1999-04-01 |
| NO20001497L (no) | 2000-05-25 |
| IL135242A0 (en) | 2001-05-20 |
| DE69841723D1 (de) | 2010-07-29 |
| AU9581698A (en) | 1999-04-12 |
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