CN100582230C - 通过载体构建体与细胞dna的非同源重组来表达内源基因 - Google Patents

通过载体构建体与细胞dna的非同源重组来表达内源基因 Download PDF

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CN100582230C
CN100582230C CN98811571A CN98811571A CN100582230C CN 100582230 C CN100582230 C CN 100582230C CN 98811571 A CN98811571 A CN 98811571A CN 98811571 A CN98811571 A CN 98811571A CN 100582230 C CN100582230 C CN 100582230C
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cell
gene
sequence
construct
expression
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CN1280615A (zh
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约翰·J·哈林顿
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Athersys Inc
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Athersys Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
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  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN98811571A 1997-09-26 1998-09-25 通过载体构建体与细胞dna的非同源重组来表达内源基因 Expired - Lifetime CN100582230C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US94122397A 1997-09-26 1997-09-26
US08/941,223 1997-09-26
US15964398A 1998-09-24 1998-09-24
US09/159,643 1998-09-24

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CN1280615A CN1280615A (zh) 2001-01-17
CN100582230C true CN100582230C (zh) 2010-01-20

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CN98811571A Expired - Lifetime CN100582230C (zh) 1997-09-26 1998-09-25 通过载体构建体与细胞dna的非同源重组来表达内源基因

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EP (1) EP1017803B1 (enExample)
JP (1) JP5377806B2 (enExample)
KR (1) KR20010024296A (enExample)
CN (1) CN100582230C (enExample)
AT (1) ATE471373T1 (enExample)
AU (1) AU750025B2 (enExample)
BR (1) BR9812540A (enExample)
CA (1) CA2304642C (enExample)
DE (1) DE69841723D1 (enExample)
DK (1) DK1017803T3 (enExample)
IL (2) IL135242A0 (enExample)
NO (1) NO20001497L (enExample)
NZ (1) NZ504185A (enExample)
WO (1) WO1999015650A1 (enExample)

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US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
JP2004501601A (ja) * 1999-02-19 2004-01-22 アサーシス，インコーポレイテッド 標的化によらない内在性遺伝子の活性化組成物および活性化方法
AU783767B2 (en) 1999-10-14 2005-12-01 Takara Bio Usa, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
US7964713B2 (en) 2000-06-28 2011-06-21 Amgen Inc. Nucleic acid molecules encoding thymic stromal lympopoietin receptor proteins and a process for producing the encoded polypeptides thereof
GB0018876D0 (en) * 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
WO2002036760A2 (en) * 2000-10-30 2002-05-10 Novozymes Biotech, Inc. Methods for expressing endogenous genes by restriction enzyme mediated integration
WO2003040324A2 (en) * 2001-11-02 2003-05-15 Athersys, Inc. Methods for mutating genes in cells and animals using insertional mutagenesis
WO2003054158A2 (en) 2001-12-19 2003-07-03 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
US7662924B2 (en) 2002-02-22 2010-02-16 The Board Of Trustees Of The University Of Illinois Beta chain-associated regulator of apoptosis
ES2609292T3 (es) 2002-08-21 2017-04-19 Revivicor, Inc. Animales porcinos que carecen de cualquier expresión de alfa 1,3 galactosiltransferasa funcional
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2004071462A2 (en) 2003-02-12 2004-08-26 Johns Hopkins University Methods and compositions for treatment of viral infections based on tsg101-vps28 interaction
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
JP2007529278A (ja) 2004-03-17 2007-10-25 レビビコア， インコーポレイテッド 機能的α１，３ガラクトシルトランスフェラーゼを欠く動物に由来する組織生成物
EP1732944B1 (en) 2004-04-07 2012-09-05 The University of Chicago Monomeric red fluorescent proteins
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
CA2585098C (en) 2004-10-22 2018-12-18 Revivicor, Inc. Porcine genomic kappa and lambda light chain sequences
PA8660701A1 (es) 2005-02-04 2006-09-22 Pfizer Prod Inc Agonistas de pyy y sus usos
EP3683230A1 (en) 2005-05-12 2020-07-22 ZymoGenetics, Inc. Compositions and methods for modulating immune responses
AU2006292827B2 (en) 2005-08-09 2013-02-14 Revivicor, Inc. Transgenic ungulates expressing CTLA4-IG and uses thereof
WO2007053732A2 (en) 2005-11-01 2007-05-10 Mayo Foundation For Medical Education And Research Promoter polymorphisms of the blys gene and use in diagnostic methods
US8014957B2 (en) 2005-12-15 2011-09-06 Fred Hutchinson Cancer Research Center Genes associated with progression and response in chronic myeloid leukemia and uses thereof
DK2046826T3 (da) 2006-07-24 2011-10-24 Biorexis Pharmaceutical Corp Exendin-fusionsproteiner
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US8679749B2 (en) 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
US8003325B2 (en) 2007-11-30 2011-08-23 Mayo Foundation For Medical Education And Research Polymorphisms of the BLyS gene and use in diagnostic methods
EP2525808A2 (en) 2010-01-19 2012-11-28 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for male reproductive disorders
CN102669057B (zh) * 2011-03-18 2014-11-26 上海南方模式生物科技发展有限公司 一种调控动物内源基因表达的遗传修饰方法
KR101948248B1 (ko) * 2011-11-18 2019-02-14 한국생명공학연구원 단백질 발현 미세 조절방법
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
US10052364B2 (en) 2013-03-15 2018-08-21 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for cognitive disorders
CA3208184A1 (en) * 2014-03-14 2015-09-17 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
WO2016081728A1 (en) 2014-11-19 2016-05-26 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for frailty associated with aging
CN112384624A (zh) * 2018-05-15 2021-02-19 杜克大学 用于对阿克曼菌进行基因操纵的系统和方法

Citations (5)

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US5204939A (en) * 1989-12-14 1993-04-20 Fujitsu Limited Rule base processing system and rule evaluation control method therein
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
WO1995031560A1 (en) * 1994-05-13 1995-11-23 Transkaryotic Therapies, Inc. Dna construct for effecting homologous recombination and uses thereof
WO1996029411A1 (en) * 1995-03-17 1996-09-26 Transkaryotic Therapies, Inc. Protein production and delivery
US5578461A (en) * 1989-11-06 1996-11-26 Cell Genesys, Inc. Gene manipulation and expression using genomic elements

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US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
US5561053A (en) * 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
JP2004501601A (ja) * 1999-02-19 2004-01-22 アサーシス，インコーポレイテッド 標的化によらない内在性遺伝子の活性化組成物および活性化方法

Patent Citations (6)

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US5578461A (en) * 1989-11-06 1996-11-26 Cell Genesys, Inc. Gene manipulation and expression using genomic elements
US5204939A (en) * 1989-12-14 1993-04-20 Fujitsu Limited Rule base processing system and rule evaluation control method therein
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
WO1995031560A1 (en) * 1994-05-13 1995-11-23 Transkaryotic Therapies, Inc. Dna construct for effecting homologous recombination and uses thereof
WO1996029411A1 (en) * 1995-03-17 1996-09-26 Transkaryotic Therapies, Inc. Protein production and delivery

Non-Patent Citations (1)

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Publication number Publication date
NO20001497L (no) 2000-05-25
DK1017803T3 (da) 2010-08-23
EP1017803B1 (en) 2010-06-16
EP1017803A4 (en) 2002-01-09
BR9812540A (pt) 2001-12-26
CN1280615A (zh) 2001-01-17
EP1017803A1 (en) 2000-07-12
JP2003535564A (ja) 2003-12-02
ATE471373T1 (de) 2010-07-15
AU750025B2 (en) 2002-07-11
CA2304642C (en) 2014-11-18
AU9581698A (en) 1999-04-12
CA2304642A1 (en) 1999-04-01
HK1027375A1 (en) 2001-01-12
IL135242A0 (en) 2001-05-20
WO1999015650A1 (en) 1999-04-01
DE69841723D1 (de) 2010-07-29
NO20001497D0 (no) 2000-03-22
KR20010024296A (ko) 2001-03-26
WO1999015650A9 (en) 2000-09-08
NZ504185A (en) 2003-02-28
JP5377806B2 (ja) 2013-12-25
IL135242A (en) 2011-06-30

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